LOCUS       sequence-240775-       10472 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral Expression Vektor with CSF2RB-A455D mutant cDNA and IRES 
            eGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10472)
  AUTHORS   Labuhn M, Perkins K, Matzk S, Varghese L, Garnett C, Papaemmanuil E,
            Metzner M, Kennedy A, Amstislavskiy V, Risch T, Bhayadia R, 
            Samulowski D, Hernandez DC, Stoilova B, Iotchkova V, Oppermann U, 
            Scheer C, Yoshida K, Schwarzer A, Taub JW, Crispino JD, Weiss MJ, 
            Hayashi Y, Taga T, Ito E, Ogawa S, Reinhardt D, Yaspo ML, Campbell 
            PJ, Roberts I, Constantinescu SN, Vyas P, Heckl D, Klusmann JH
  TITLE     Mechanisms of Progression of Myeloid Preleukemia to Transformed 
            Myeloid Leukemia in Children with Down Syndrome.
  JOURNAL   Cancer Cell. 2019 Aug 12;36(2):123-138.e10. doi: 
            10.1016/j.ccell.2019.06.007. Epub 2019 Jul 11.
  PUBMED    31303423
REFERENCE   2  (bases 1 to 10472)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2019.06.007"; journalName: "Cancer Cell"; date: 
            "2019-08-12- 12"; volume: "36"; issue: "2"; pages: "123-138.e10"
COMMENT     Sequence Label: LeGO-iG-CSF2RB(A455D)
FEATURES             Location/Qualifiers
     source          1..10472
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1686..1919
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2104..2148
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2446..2563
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     2622..2638
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2680..2713)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        2784..3190
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             5508..5519
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     misc_feature    join(5919..6493,6494..6496,6497..6505)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6086..6103)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6313..6332
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(6506..6508,6509..6511,6512..7225)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6506..6508,6509..6511,6512..7222)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6551..6572)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6812..6831)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7159..7180
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(7272..7305)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    7361..7949
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7414..7434)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7832..7843)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7952..7968)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     LTR             8478..8658
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(8720..9308)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8800..8819)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9479..10270,10271..10339))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10102..10121
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10340..10444)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg gccgcgctga tcttcagacc tggaggagga
     1561 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
     1621 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
     1681 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
     1741 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
     1801 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     1861 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     1921 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     1981 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     2041 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     2101 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     2161 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     2221 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     2281 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
     2341 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatcggca
     2401 ctgcgtgcgc caattctgca gacaaatggc agtattcatc cacaatttta aaagaaaagg
     2461 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     2521 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
     2581 cagcagagat ccagtttggt tagtaccggg cccgctctag tcgaggtcga cggtatcgat
     2641 aagctcgctt cacgagattc cagcaggtcg agggacctaa taacttcgta tagcatacat
     2701 tatacgaagt tatattaagg gttccaagct taagcggccg gccgctgaaa gaccccacct
     2761 gtaggtttgg caagctagct gcagtaacgc cattttgcaa ggcatggaaa aataccaaac
     2821 caagaataga gaagttcaga tcaagggcgg gtacatgaaa atagctaacg ttgggccaaa
     2881 caggatatct gcggtgagca gtttcggccc cggcccgggg ccaagaacag atggtcaccg
     2941 cagtttcggc cccggcccga ggccaagaac agatggtccc cagatatggc ccaaccctca
     3001 gcagtttctt aagacccatc agatgtttcc aggctccccc aaggacctga aatgaccctg
     3061 cgccttattt gaattaacca atcagcctgc ttctcgcttc tgttcgcgcg cttctgcttc
     3121 ccgagctcta taaaagagct cacaacccct cactcggcgc gccagtcctc cgattgactg
     3181 agtcgcccgg atccatggtg ctggcccagg gcctgctgtc tatggccctg ctggccctgt
     3241 gctgggagag aagcctcgca ggcgccgagg aaaccatccc cctgcagacc ctgcggtgct
     3301 acaacgacta caccagccac atcacctgta gatgggccga cacccaggac gcccagcggc
     3361 tggtcaacgt gaccctgatc agaagagtga acgaggacct gctggaaccc gtgtcctgcg
     3421 acctgagcga cgacatgcct tggagcgcct gcccccaccc tagatgtgtg cctcggagat
     3481 gcgtgatccc ctgccagagc ttcgtggtca ccgacgtgga ctacttcagc ttccagcccg
     3541 accggcctct gggcaccaga ctgaccgtga cactgaccca gcacgtgcag ccccccgagc
     3601 ccagggatct ccagatctct accgaccagg accacttcct gctgacttgg agcgtggccc
     3661 tcggcagccc tcagagccat tggctgagcc ctggcgacct ggaattcgag gtggtgtaca
     3721 agcggctgca ggacagctgg gaggacgctg ccatcctgct gagcaacacc agccaggcca
     3781 ccctgggccc cgagcacctg atgcctagca gcacctacgt ggccagagtg cggaccagac
     3841 tggcccctgg cagcaggctg agcggcagac ctagcaagtg gtcccccgaa gtgtgctggg
     3901 acagccagcc aggcgacgag gcccagcccc agaacctgga atgcttcttc gacggcgctg
     3961 ccgtgctgag ctgcagctgg gaagtgcgga aagaggtggc cagcagcgtc agcttcggcc
     4021 tgttctacaa gcccagcccc gacgccggcg aagaggaatg cagcccagtg ctgagagagg
     4081 gcctgggctc cctgcacacc cggcaccact gtcagatccc cgtgcccgat cctgccaccc
     4141 acggccagta catcgtgtcc gtgcagccca gacgggccga gaagcacatc aagagcagcg
     4201 tgaacatcca gatggcccct cccagcctga acgtgaccaa ggacggagac agctacagcc
     4261 tgagatggga gacaatgaag atgcgctacg agcacatcga ccacaccttc gagatccagt
     4321 accggaagga caccgccacc tggaaggaca gcaagaccga gacactgcag aacgcccaca
     4381 gcatggccct gcccgccctg gaacccagca ccagatactg ggccagagtc cgcgtgcgga
     4441 cctccagaac cggctacaac ggcatttgga gcgagtggtc tgaggccaga agctgggaca
     4501 ccgagagcgt gctgcctatg tgggtgctgg ctctgatcgt gatcttcctg acaatcgatg
     4561 tgctgctggc actgcggttc tgcggcatct acggctaccg gctgcggcgg aagtgggagg
     4621 aaaagatccc caaccccagc aagagccacc tgttccagaa cggctccgcc gagctgtggc
     4681 ctcctggctc catgagcgcc ttcaccagcg gctctccacc ccaccagggc ccttggggca
     4741 gcagatttcc tgagctggaa ggcgtgttcc ccgtgggctt cggcgacagc gaggtgtccc
     4801 ctctgaccat cgaggacccc aagcacgtgt gcgacccccc cagcggacct gatacaaccc
     4861 ctgccgccag cgacctgcct accgagcagc ctccatctcc ccagcctgga cctccagccg
     4921 ccagccacac accagagaag caggccagca gcttcgactt caacggcccc tacctgggcc
     4981 ctccccacag cagaagcctg cccgatatcc tcggccagcc cgagcctcct caggaaggcg
     5041 gcagccagaa gtcccctcca cccggcagcc tggaatacct gtgtctgcct gccggcggac
     5101 aggtgcagct ggtgcctctc gctcaggcca tgggacctgg acaggctgtg gaagtggaaa
     5161 gaaggcctag ccagggcgct gccggctccc catctctgga atctggcgga ggacctgccc
     5221 ctcctgccct gggacctaga gtgggcggcc aggaccagaa agacagcccc gtggccatcc
     5281 ccatgagcag cggcgataca gaggaccctg gcgtggccag cggctacgtg tccagcgccg
     5341 atctggtgtt cacccccaac agcggcgcca gctccgtgtc cctggtgcct tctctgggcc
     5401 tgcccagcga ccagacccct agcctgtgtc ctggactggc ctctggacct cctggcgccc
     5461 ctggacctgt gaagtccggc ttcgagggct acgtggaact gccccccatc gagggcagaa
     5521 gcccccggtc ccccagaaac aaccctgtgc cccctgaggc caagagcccc gtgctgaatc
     5581 ccggcgaaag acccgccgac gtgtccccca caagccctca gccagaggga ctgctggtgc
     5641 tgcagcaagt gggagactac tgcttcctgc ctggcctcgg accagggcca ctgtccctga
     5701 gaagcaagcc tagctctccc ggcccaggcc ccgagatcaa gaacctggac caggccttcc
     5761 aggtcaaaaa gcctcccgga caggccgtgc cccaggtgcc cgtgatccag ctgttcaagg
     5821 ccctgaagca gcaggactac ctgagcctgc ccccctggga agtgaacaag cccggcgaag
     5881 tctgctgagc ggccgctacg taaattccgc cccccccccc cctctccctc ccccccccct
     5941 aacgttactg gccgaagccg cttggaataa ggccggtgtg cgtttgtcta tatgttattt
     6001 tccaccatat tgccgtcttt tggcaatgtg agggcccgga aacctggccc tgtcttcttg
     6061 acgagcattc ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc
     6121 gtgaaggaag cagttcctct ggaagcttct tgaagacaaa caacgtctgt agcgaccctt
     6181 tgcaggcagc ggaacccccc acctggcgac aggtgcctct gcggccaaaa gccacgtgta
     6241 taagatacac ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg
     6301 gaaagagtca aatggctctc ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag
     6361 gtaccccatt gtatgggatc tgatctgggg cctcggtgca catgctttac atgtgtttag
     6421 tcgaggttaa aaaaacgtct aggccccccg aaccacgggg acgtggtttt cctttgaaaa
     6481 acacgatgat aatatggcca caaccatggt gagcaagggc gaggagctgt tcaccggggt
     6541 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg
     6601 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg
     6661 caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt
     6721 cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg
     6781 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga
     6841 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa
     6901 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta
     6961 tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat
     7021 cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg
     7081 ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc
     7141 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct
     7201 cggcatggac gagctgtaca agtaaagcgg ccggccgcca gcacagtggt cgaaattcgt
     7261 cgagggacct aataacttcg tatagcatac attatacgaa gttatacatg tttaagggtt
     7321 ccggttccac taggtacaat tcgatatcaa gcttatcgat aatcaacctc tggattacaa
     7381 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
     7441 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc
     7501 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg
     7561 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac
     7621 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat
     7681 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt
     7741 ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat
     7801 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc
     7861 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag
     7921 tcggatctcc ctttgggccg cctccccgca tcgataccgt cgacctcgat cgagacctag
     7981 aaaaacatgg agcaatcaca agtagcaata cagcagctac caatgctgat tgtgcctggc
     8041 tagaagcaca agaggaggag gaggtgggtt ttccagtcac acctcaggta cctttaagac
     8101 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg
     8161 aagggctaat tcactcccaa cgaagacaag atatccttga tctgtggatc taccacacac
     8221 aaggctactt ccctgattgg cagaactaca caccagggcc agggatcaga tatccactga
     8281 cctttggatg gtgctacaag ctagtaccag ttgagcaaga gaaggtagaa gaagccaatg
     8341 aaggagagaa cacccgcttg ttacaccctg tgagcctgca tgggatggat gacccggaga
     8401 gagaagtatt agagtggagg tttgacagcc gcctagcatt tcatcacatg gcccgagagc
     8461 tgcatccgga ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     8521 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     8581 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     8641 tgtggaaaat ctctagcagc atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     8701 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     8761 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     8821 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     8881 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     8941 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     9001 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     9061 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     9121 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     9181 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     9241 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     9301 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     9361 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     9421 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     9481 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     9541 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     9601 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     9661 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     9721 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     9781 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     9841 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     9901 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     9961 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
    10021 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
    10081 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
    10141 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
    10201 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
    10261 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
    10321 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
    10381 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
    10441 cgcgcacatt tccccgaaaa gtgccacctg ac
//
