LOCUS       sequence-24081-e        1092 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1092)
  TITLE     T4 Lysozyme Plasmids - Tray 2
REFERENCE   2  (bases 1 to 1092)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: T4 Lysozyme R52V N53A T54S N55G V57T WT*
FEATURES             Location/Qualifiers
     source          1..1092
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    725..741
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(749..755,756..771,772..777))
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    820..836
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(844..850,851..866,867..872))
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
ORIGIN
        1 ctantggaaa aagcccgctc attaggcggg ctcagatctg ctcatgtttg acagcttatc
       61 atcgataagc ttcggcaata aattcttgat atgttttcat atgtttcctt taaatatttt
      121 aattagtaat tgtctattca agtaattgtg aatatactat cacaattcca agagaaagta
      181 aacagcttta tagattttta tacgcgtccc aagtgccagt tctaaacgtt gtaatgactc
      241 gttttgcgcg attaggtgtt tgattatacc atctactttt agctaagtta actgctgctt
      301 catcccagcg tttttgttga agcatacgta aagagttagt aaatcctgcc acaccggttt
      361 ctcccatttg gaaaaccata ttaatcaatg cagcgcgacg aaccgcatca agagaatcat
      421 aaaccggttt taatttagca tttctcagaa ttccgcgaac agcagcatca acatcctgat
      481 taaagagttt ttcagcctca tcttttgtaa ttacaccatt agtattacgc ccaatagctt
      541 tatctaattc agatttagca gcattagaat ttccatctac tttaagcgta tgaccgatgc
      601 caatagtgta atagccttct gtgtctttat agattttaag tctaagacct tcatctatac
      661 gtaacatttc aaatatattc ataatacctc ctaagcatcg atggatcctg tttcctgtgt
      721 gaaattgtta tccgctcaca attccacaca ttatacgagc cgatgattaa ttgtcaacag
      781 ggggatgggg agtaagctga tcctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
      841 acacattata cgagccgatg attaattgtc aacaggggga tggggagtaa gctcatcgat
      901 ggatcgancn gnttcccgng ggnaaatggt anccgcncnc nantnccncn cnttancnag
      961 ccggaagcnn aangggnaan gccggggggg gccnaangng ggnncnanct tanntnantg
     1021 ggnntgcncn cnnggcccgc tttccnnncg ggaaancngn nnggccggnn nccnnnnaag
     1081 ggggnaaacn tt
//
