LOCUS       sequence-24082-e        1086 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1086)
  TITLE     T4 Lysozyme Plasmids - Tray 2
REFERENCE   2  (bases 1 to 1086)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: T4 Lysozyme 1001A
FEATURES             Location/Qualifiers
     source          1..1086
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    721..737
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(745..751,752..767,768..773))
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    816..832
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(840..846,847..862,863..868))
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    924..940
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(948..954,955..972,973..978))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
ORIGIN
        1 ttngaaaagc ccgctcatta ggcgggctca gatctgctca tgtttgacag cttatcatcg
       61 ataagcttcg gcaataaatt cttgatatgt tttcatatgt ttcctttaaa tattttaatt
      121 agtaattgtc tattcaagta attgtgaata tactatcaca attccaagag aaagtaaaca
      181 gctttataga tttttatacg cgtcccaagt gccagttcta aacgttgtaa tgactcgttt
      241 tgcgcgatta ggtgtttgat tataccatct acttttagct aaggcagctg ctgccgcagc
      301 ccagcgtttt tgttgaagca tacgtaaaga gttagtaaat cctgccacac cggtttctcc
      361 catttggaaa accatattaa tcaatgcagc gcgacgaacc gcatcaagag aatcataaac
      421 cggttttaat ttagcatttc tcagaattcc gcgaacagca gcatcaacat cctgattaaa
      481 gagtttttca gcctcatctt ttgtaattac accattagta ttacgcccaa tagctgcagc
      541 taatgcagct ttagcagcag caagtgatgg actttttgta agcaaatgac cgatgccaat
      601 agtgtaatag ccttctgtgt ctttatagat tttaagtcta agaccttcat ctatacgtaa
      661 catttcaaat atattcataa tacctcctaa gcatcgatgg atcctgtttc ctgtgtgaaa
      721 ttgttatccg ctcacaattc cacacattat acgagccgat gattaattgt caacaggggg
      781 atggggagta agctgatcct gtttcctgtg tgaaattgtt atccgctcac aattccacac
      841 attatacgag ccgatgatta attgtcaaca gggggatggg gagtaagctc atcgatggat
      901 cgatcnggtt tccngggggg aaattgttat ccgctcacaa ttccncacat tatacgagcc
      961 ggaagcataa agngtaaagc ccnggggggg cctaaagnng ggagctaacn ttacnttaat
     1021 tgggttgcgc cccccggccc gcntttccag tcggggaaac ctgntcgngn ccaggacacc
     1081 atcgaa
//
