LOCUS       sequence-240827-        5749 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transfected into cell of interest for membrane expression. The 
            encoded mgSrtA could enable labeling of the interacting cells which 
            is useful for recording cell-cell interactions..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5749)
  AUTHORS   Ge Y, Chen L, Liu S, Zhao J, Zhang H, Chen PR
  TITLE     Enzyme-Mediated Intercellular Proximity Labeling for Detecting 
            Cell-Cell Interactions.
  JOURNAL   J Am Chem Soc. 2019 Feb 6;141(5):1833-1837. doi: 
            10.1021/jacs.8b10286. Epub 2019 Jan 29.
  PUBMED    30676735
REFERENCE   2  (bases 1 to 5749)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/jacs.8b10286"; journalName: "J Am Chem Soc"; date: 
            "2019-02-6- 6"; volume: "141"; issue: "5"; pages: "1833-1837"
COMMENT     Sequence Label: pDisplay-mgSrtA
FEATURES             Location/Qualifiers
     source          1..5749
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        10..389
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        390..593
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     544..564
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     638..657
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        638..656
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     sig_peptide     737..796
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTG"
     CDS             797..820
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1331..1477
                     /codon_start=1
                     /product="transmembrane domain from platelet derived growth
                     factor receptor beta"
                     /label=PDGFR-beta TM domain
                     /translation="AVGQDTQEVIVVPHSLPFKVVVISAILALVVLTIISLIILIMLWQ
                     KKPR"
     primer_bind     complement(1499..1516)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1505..1729
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(1861..2449)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1941..1960)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    2778..2825
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     2850..2869
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(3057..3845)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDQEHQGL
                     APAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIALA
                     TRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3168..3187)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     3772..3791
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(3880..4240)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3894..4032
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        4049..4240
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     4197..4217
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     CDS             complement(join(4300..5091,5092..5160))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4923..4942
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5161..5265)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      5292..5747
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5379..5398)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5589..5610
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gcgcgcgttg acattgatta ttgactagtt attaatagta atcaattacg gggtcattag
       61 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
      121 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
      181 caatagggac tttccattga cgtcaatggg tggactattt acggtaaact gcccacttgg
      241 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
      301 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
      361 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
      421 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
      481 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
      541 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctctctggc
      601 taactagaga acccactgct tactggctta tcgaaattaa tacgactcac tatagggaga
      661 cccaagcttg gtaccgagct cggatccact agtaacggcc gccagtgtgc tggaattcgg
      721 cttggggata tccaccatgg agacagacac actcctgcta tgggtactgc tgctctgggt
      781 tccaggttcc actggtgact acaaggacga cgacgacaag ggaagcggac tggagcaggc
      841 aaagccacag atacccaagg acaagagcaa ggtggcagga tacatagaga taccagacgc
      901 tgacataaaa gagcccgtgt accccggacc cgccacaaga gaacaactga acagaggagt
      961 gtccttcgcc aaagaaaacc agagcctgga cgaccaaaac atcagcatcg ccggccacac
     1021 cttcatcgga agacccaact accagttcac caacctgaag gccgccaaaa agggaagcat
     1081 ggtgtacttc aaggtgggca acgaaaccag aaaatacaag atgacctcca taagaaacgt
     1141 gaagcccacc gccgtgggcg tcctggacga acaaaaggga aaggacaagc agctgaccct
     1201 gatcacctgc gatgatctga acagagaaac cggcgtgtgg gagaccagaa aaatcctggt
     1261 ggccacagaa gtgaagggat ctacaagcgg gagcgggaag cccggcagcg gagaaggaag
     1321 cacaaaggga gctgtgggcc aggacacgca ggaggtcatc gtggtgccac actccttgcc
     1381 ctttaaggtg gtggtgatct cagccatcct ggccctggtg gtgctcacca tcatctccct
     1441 tatcatcctc atcatgcttt ggcagaagaa gccacgttag gcggccgctc gagatcagcc
     1501 tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg
     1561 accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat
     1621 tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag
     1681 gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg
     1741 gaaagaacca gtggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     1801 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     1861 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     1921 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     1981 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     2041 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     2101 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     2161 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     2221 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     2281 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
     2341 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     2401 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     2461 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     2521 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     2581 atcaatctaa agtatatatg agtaacctga ggctatggca gggcctgccg ccccgacgtt
     2641 ggctgcgagc cctgggcctt cacccgaact tggggggtgg ggtggggaaa aggaagaaac
     2701 gcgggcgtat tggccccaat ggggtctcgg tggggtatcg acagagtgcc agccctggga
     2761 ccgaaccccg cgtttatgaa caaacgaccc aacaccgtgc gttttattct gtctttttat
     2821 tgccgtcata gcgcgggttc cttccggtat tgtctccttc cgtgtttcag ttagcctccc
     2881 cctagggtgg gcgaagaact ccagcatgag atccccgcgc tggaggatca tccagccggc
     2941 gtcccggaaa acgattccga agcccaacct ttcatagaag gcggcggtgg aatcgaaatc
     3001 tcgtgatggc aggttgggcg tcgcttggtc ggtcatttcg aaccccagag tcccgctcag
     3061 aagaactcgt caagaaggcg atagaaggcg atgcgctgcg aatcgggagc ggcgataccg
     3121 taaagcacga ggaagcggtc agcccattcg ccgccaagct cttcagcaat atcacgggta
     3181 gccaacgcta tgtcctgata gcggtccgcc acacccagcc ggccacagtc gatgaatcca
     3241 gaaaagcggc cattttccac catgatattc ggcaagcagg catcgccatg ggtcacgacg
     3301 agatcctcgc cgtcgggcat gctcgccttg agcctggcga acagttcggc tggcgcgagc
     3361 ccctgatgct cttgatcatc ctgatcgaca agaccggctt ccatccgagt acgtgctcgc
     3421 tcgatgcgat gtttcgcttg gtggtcgaat gggcaggtag ccggatcaag cgtatgcagc
     3481 cgccgcattg catcagccat gatggatact ttctcggcag gagcaaggtg agatgacagg
     3541 agatcctgcc ccggcacttc gcccaatagc agccagtccc ttcccgcttc agtgacaacg
     3601 tcgagcacag ctgcgcaagg aacgcccgtc gtggccagcc acgatagccg cgctgcctcg
     3661 tcttgcagtt cattcagggc accggacagg tcggtcttga caaaaagaac cgggcgcccc
     3721 tgcgctgaca gccggaacac ggcggcatca gagcagccga ttgtctgttg tgcccagtca
     3781 tagccgaata gcctctccac ccaagcggcc ggagaacctg cgtgcaatcc atcttgttca
     3841 atcatgcgaa acgatcctca tcctgtctct tgatcgatct ttgcaaaagc ctaggcctcc
     3901 aaaaaagcct cctcactact tctggaatag ctcagaggcc gaggaggcgg cctcggcctc
     3961 tgcataaata aaaaaaatta gtcagccatg gggcggagaa tgggcggaac tgggcggagt
     4021 taggggcggg atgggcggag ttaggggcgg gactatggtt gctgactaat tgagatgcat
     4081 gctttgcata cttctgcctg ctggggagcc tggggacttt ccacacctgg ttgctgacta
     4141 attgagatgc atgctttgca tacttctgcc tgctggggag cctggggact ttccacaccc
     4201 taactgacac acattccaca gctggttctt tccgcctcag gactcttcct ttttcaataa
     4261 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     4321 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     4381 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     4441 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     4501 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     4561 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     4621 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     4681 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     4741 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     4801 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     4861 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     4921 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     4981 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     5041 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     5101 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     5161 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     5221 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     5281 agtgccacct gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     5341 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     5401 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     5461 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     5521 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     5581 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     5641 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     5701 acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttac
//
