LOCUS       sequence-24085-e        1105 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1105)
  TITLE     T4 Lysozyme Plasmids - Tray 2
REFERENCE   2  (bases 1 to 1105)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: T4 Lysozyme N40-[AA] WT*
FEATURES             Location/Qualifiers
     source          1..1105
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    728..744
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(752..758,759..774,775..780))
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    823..839
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(847..853,854..869,870..875))
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
ORIGIN
        1 ctangaaaag cccgctcatt aggcgggctc agatctgctc atgtttgaca gcttatcatc
       61 gataagcttc ggcaataaat tcttgatatg ttttcatatg tttcctttaa atattttaat
      121 tagtaattgt ctattcaagt aattgtgaat atactatcac aattccaaga gaaagtaaac
      181 agctttatag atttttatac gcgtcccaag tgccagttct aaacgttgta atgactcgtt
      241 ttgcgcgatt aggtgtttga ttataccatc tacttttagc taagttaact gctgcttcat
      301 cccagcgttt ttgttgaagc atacgtaaag agttagtaaa tcctgccaca ccggtttctc
      361 ccatttggaa aaccatatta atcaatgcag cgcgacgaac cgcatcaaga gaatcataaa
      421 ccggttttaa tttagcattt ctcagaattc cgcgaacagc agcatcaaca tcctgattaa
      481 agagtttttc agcctcatct tttgtaatta caccattagt attacgccca atagctttat
      541 ctaattcaga tttagcagct gcagcattaa gtgatggact ttttgtaagc aaatgaccga
      601 tgccaatagt gtaatagcct tctgtgtctt tatagatttt aagtctaaga ccttcatcta
      661 tacgtaacat ttcaaatata ttcataatac ctcctaagca tcgatggatc ctgtttcctg
      721 tgtgaaattg ttatccgctc acaattccac acattatacg agccgatgat taattgtcaa
      781 cagggggatg gggagtaagc tgatcctgtt tcctgtgtga aattgttatc cgctcacaat
      841 tccacacatt atacgagccg atgattaatt gtcaacaggg ggatggggag taagctcatc
      901 gatggatcga ccgggnntnn ccggggggna aaatngnnnn ccnncncnna attccccncn
      961 tttanacnan nccggnannc nnaangnggn aaagcccggg ggggnccnaa ngngnggagc
     1021 naacttacnn ttaattgcgn tggcncncnc nggcccnctt tcccngncgg gaaanccngn
     1081 nngggccngg annccnncnn aaggg
//
