LOCUS       sequence-240862-        1029 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses GST-RPN13 (19-132) R104E in E.coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1029)
  AUTHORS   Hemmis CW, Heard SC, Hill CP
  TITLE     Phosphorylation of Tyr950 in the proteasome scaffolding protein RPN2
            modulates its interaction with the ubiquitin receptor RPN13.
  JOURNAL   J Biol Chem. 2019 May 7. pii: AC119.008881. doi: 
            10.1074/jbc.AC119.008881.
  PUBMED    31064842
REFERENCE   2  (bases 1 to 1029)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2019 May 7. pii: AC119.008881. doi: 10.1074/jbc.AC119.008881."
COMMENT     Sequence Label: pET151-GST-PP-RPN13 (19-132) R104E
FEATURES             Location/Qualifiers
     source          1..1029
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..654
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     612..634
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     CDS             661..684
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
ORIGIN
        1 atgtccccta tactaggtta ttggaaaatt aagggccttg tgcaacccac tcgacttctt
       61 ttggaatatc ttgaagaaaa atatgaagag catttgtatg agcgcgatga aggtgataaa
      121 tggcgaaaca aaaagtttga attgggtttg gagtttccca atcttcctta ttatattgat
      181 ggtgatgtta aattaacaca gtctatggcc atcatacgtt atatagctga caagcacaac
      241 atgttgggtg gttgtccaaa agagcgtgca gagatttcaa tgcttgaagg agcggttttg
      301 gatattagat acggtgtttc gagaattgca tatagtaaag actttgaaac tctcaaagtt
      361 gattttctta gcaagctacc tgaaatgctg aaaatgttcg aagatcgttt atgtcataaa
      421 acatatttaa atggtgatca tgtaacccat cctgacttca tgttgtatga cgctcttgat
      481 gttgttttat acatggaccc aatgtgcctg gatgcgttcc caaaattagt ttgttttaaa
      541 aaacgtattg aagctatccc acaaattgat aagtacttga aatccagcaa gtatatagca
      601 tggcctttgc agggctggca agccacgttt ggtggtggcg accatcctcc aaaatcggat
      661 ctggaagttc tgttccaggg gccgtccaac aagtacttgg tggagtttcg ggcgggaaag
      721 atgtccctga aggggaccac cgtgactccg gataagcgga aagggctggt gtacattcag
      781 cagacggacg actcgcttat tcacttctgc tggaaggaca ggacgtccgg gaacgtggaa
      841 gacgacttga tcatcttccc tgacgactgt gagttcaagc gggtgccgca gtgccccagc
      901 gggagggtct acgtgctgaa gttcaaggca gggtccaagg agcttttctt ctggatgcag
      961 gaacccaaga cagaccagga tgaggagcat tgccggaaag tcaacgagta tctgaacaac
     1021 cccccgtaa
//
