LOCUS       sequence-24087-e         990 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 990)
  TITLE     T4 Lysozyme Plasmids - Tray 2
REFERENCE   2  (bases 1 to 990)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: T4 Lysozyme N40-[AAAA] WT*
FEATURES             Location/Qualifiers
     source          1..990
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(725..747)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    736..752
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(760..766,767..782,783..788))
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
ORIGIN
        1 gctangnaaa agcccgctca ttaggcgggc tcagatctgc tcatgtttga cagcttatca
       61 tcgataagct tcggcaataa attcttgata tgttttcata tgtttccttt aaatatttta
      121 attagtaatt gtctattcaa gtaattgtga atatactatc acaattccaa gagaaagtaa
      181 acagctttat agatttttat acgcgtccca agtgccagtt ctaaacgttg taatgactcg
      241 ttttgcgcga ttaggtgttt gattatacca tctactttta gctaagttaa ctgctgcttc
      301 atcccagcgt ttttgttgaa gcatacgtaa agagttagta aatcctgcca caccggtttc
      361 tcccatttgg aaaaccatat taatcaatgc agcgcgacga accgcatcaa gagaatcata
      421 aaccggtttt aatttagcat ttctcagaat tccgcgaaca gcagcatcaa catcctgatt
      481 aaagagtttt tcagcctcat cttttgtaat tacaccatta gtattacgcc caatagcttt
      541 atctaattca gatttagcag ctgcagctgc agcattaagt gatggacttt ttgtaagcaa
      601 atgaccgatg ccaatagtgt aatagccttc tgtgtcttta tagattttaa gtctaagacc
      661 ttcatctata cgtaacattt caaatatatt cataatacct cctaagcatc gatggatcct
      721 gtttcctgtg tgaaattgtt atccgctcac aattccacac attatacgag ccgatgatta
      781 attgtcaaca ggggggatgg gggagtaagc tgancctgtt tcctgggggg aaattgttat
      841 ccnctcacaa ttccncacat tatacgagcc gatgnattaa ntngtcnacn gggggggang
      901 ggggaggnaa gctcnncgan ggancgancc ngnttnccng gggggaaatt gntnanccnc
      961 cccnantccc cncnttnnac nagccggaag
//
