LOCUS       sequence-240876-        9292 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect cell expression vector for His6, Spy tagged hsDDB1∆B.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9292)
  AUTHORS   Nowak RP, DeAngelo SL, Buckley D, He Z, Donovan KA, An J, Safaee N, 
            Jedrychowski MP, Ponthier CM, Ishoey M, Zhang T, Mancias JD, Gray 
            NS, Bradner JE, Fischer ES
  TITLE     Plasticity in binding confers selectivity in ligand-induced protein 
            degradation.
  JOURNAL   Nat Chem Biol. 2018 Jul;14(7):706-714. doi: 
            10.1038/s41589-018-0055-y. Epub 2018 Jun 11.
  PUBMED    29892083
REFERENCE   2  (bases 1 to 9292)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41589-018-0055-y"; journalName: "Nat Chem Biol"; date: 
            "2018-07"; volume: "14"; issue: "7"; pages: "706-714"
COMMENT     Sequence Label: pESF140_HisSpy_DDB1∆B
FEATURES             Location/Qualifiers
     source          1..9292
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1342..1797
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1429..1448)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1639..1660
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(1814..1833)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1933..1955
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1993..2011)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2079..2183
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2184..2252,2253..3044)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2402..2421)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3215..3803
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3704..3723
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3957..3974
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_recomb     4366..5193
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     CDS             4366..4490
                     /codon_start=3
                     /product="baculovirus late expression factor 2"
                     /label=lef2
                     /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
     CDS             complement(4528..5133)
                     /codon_start=1
                     /product="baculovirus ORF603 protein"
                     /label=ORF603
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV
                     IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI
                     KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ
                     INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR"
     protein_bind    complement(5197..5230)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    5420..5468
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     5493..5512
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(5546..6223)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Strongin et al., 2007)"
                     /label=DsRed-Monomer
                     /note="mammalian codon-optimized"
                     /translation="MDNTEDVIKEFMQFKVRMEGSVNGHYFEIEGEGEGKPYEGTQTAK
                     LQVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYMKLSFPEGFTWERSMNFEDGGV
                     VEVQQDSSLQDGTFIYKVKFKGVNFPADGPVMQKKTAGWEPSTEKLYPQDGVLKGEISH
                     ALKLKDGGHYTCDFKTVYKAKKPVQLPGNHYVDSKLDITNHNEDYTVVEQYEHAEARHS
                     GSQ"
     primer_bind     complement(5806..5825)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     promoter        complement(6232..6341)
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     promoter        6396..6487
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     6412..6429
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             6511..6528
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             6535..6573
                     /codon_start=1
                     /product="peptide derived from the CnaB2 adhesin domain of 
                     Streptococcus pyogenes FbaB"
                     /label=SpyTag
                     /note="forms a covalent isopeptide bond with the SpyCatcher
                     protein (Zakeri et al., 2012)"
                     /translation="AHIVMVDAYKPTK"
     misc_recomb     join(9130..9292,1..1195)
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     CDS             complement(join(9146..9292,1..1195))
                     /codon_start=2
                     /product="baculovirus capsid-associated protein"
                     /label=ORF1629
                     /note="required for viral replication"
                     /translation="PSLRQSLYNTIAYIERLLNIGTVNDSEITMLIADFYDLYSNYNIE
                     LPPPQALPRSRRPSVVQPAAPAPVPTIVREQTKPEQIIPAAPPPPPSPVPNIPAPPPPP
                     PPSMSELPPAPPMPTEPQPAAPLDDRQQLLEAIRNEKNRTRLRPVKPKTAPETSTIVEV
                     PTVLPKETFEPKPPSASPPPPPPPPPPPAPPAPPPMVDLSSAPPPPPLVDLPSEMLPPP
                     APSLSNVLSELKSGTVRLKPAQKRPQSEIIPKSSTTNLIADVLADTINRRRVAMAKSSS
                     EATSNDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVE
                     PLLQNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDL
                     IFKFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA"
ORIGIN
        1 atcctcaata gatttgtaaa ataggtttcg attagtttca aacaagggtt gtttttccga
       61 accgatggct ggactatcta atggattttc gctcaacgcc acaaaacttg ccaaatcttg
      121 tagcagcaat ctagctttgt cgatattcgt ttgtgttttg ttttgtaata aaggttcgac
      181 gtcgttcaaa atattatgcg cttttgtatt tctttcatca ctgtcgttag tgtacaattg
      241 actcgacgta aacacgttaa ataaagcttg gacatattta acatcgggcg tgttagcttt
      301 attaggccga ttatcgtcgt cgtcccaacc ctcgtcgtta gaagttgctt ccgaagacga
      361 ttttgccata gccacacgac gcctattaat tgtgtcggct aacacgtccg cgatcaaatt
      421 tgtagttgag ctttttggaa ttatttctga ttgcgggcgt ttttgggcgg gtttcaatct
      481 aactgtgccc gattttaatt cagacaacac gttagaaagc gatggtgcag gcggtggtaa
      541 catttcagac ggcaaatcta ctaatggcgg cggtggtgga gctgatgata aatctaccat
      601 cggtggaggc gcaggcgggg ctggcggcgg aggcggaggc ggaggtggtg gcggtgatgc
      661 agacggcggt ttaggctcaa atgtctcttt aggcaacaca gtcggcacct caactattgt
      721 actggtttcg ggcgccgttt ttggtttgac cggtctgaga cgagtgcgat ttttttcgtt
      781 tctaatagct tccaacaatt gttgtctgtc gtctaaaggt gcagcgggtt gaggttccgt
      841 cggcattggt ggagcgggcg gcaattcaga catcgatggt ggtggtggtg gtggaggcgc
      901 tggaatgtta ggcacgggag aaggtggtgg cggcggtgcc gccggtataa tttgttctgg
      961 tttagtttgt tcgcgcacga ttgtgggcac cggcgcaggc gccgctggct gcacaacgga
     1021 aggtcgtctg cttcgaggca gcgcttgggg tggtggcaat tcaatattat aattggaata
     1081 caaatcgtaa aaatctgcta taagcattgt aatttcgcta tcgtttaccg tgccgatatt
     1141 taacaaccgc tcaatgtaag caattgtatt gtaaagagat tgtctcaagc tcggatcgat
     1201 cccgcacgcc gataacaagc cttttcattt ttactacagc attgtagtgg cgagacactt
     1261 cgctgtcgtc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc
     1321 atacgtcaaa gcaaccatag tacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     1381 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
     1441 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
     1501 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgatttgg
     1561 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
     1621 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
     1681 cgggctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
     1741 agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaattttat
     1801 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc
     1861 caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag
     1921 ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg
     1981 cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg
     2041 tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     2101 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     2161 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     2221 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     2281 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     2341 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     2401 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     2461 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     2521 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     2581 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     2641 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     2701 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     2761 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     2821 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     2881 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     2941 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     3001 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     3061 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     3121 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     3181 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     3241 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     3301 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     3361 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     3421 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     3481 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     3541 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     3601 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     3661 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     3721 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     3781 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     3841 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     3901 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     3961 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
     4021 ggccgattca ttaatgcaga acggctccgc ccactattaa tgaaattaaa aattccaatt
     4081 ttaaaaaacg cagcaagaga aacatttgta tgaaagaatg cgtagaagga aagaaaaatg
     4141 tcgtcgacat gctgaacaac aagattaata tgcctccgtg tataaaaaaa atattgaacg
     4201 atttgaaaga aaacaatgta ccgcgcggcg gtatgtacag gaagaggttt atactaaact
     4261 gttacattgc aaacgtggtt tcgtgtgcca agtgtgaaaa ccgatgttta atcaaggctc
     4321 tgacgcattt ctacaaccac gactccaagt gtgtgggtga agtcatgcat cttttaatca
     4381 aatcccaaga tgtgtataaa ccaccaaact gccaaaaaat gaaaactgtc gacaagctct
     4441 gtccgtttgc tggcaactgc aagggtctca atcctatttg taattattga ataataaaac
     4501 aattataaat gtcaaatttg ttttttatta acgatacaaa ccaaacgcaa caagaacatt
     4561 tgtagtatta tctataattg aaaacgcgta gttataatcg ctgaggtaat atttaaaatc
     4621 attttcaaat gattcacagt taatttgcga caatataatt ttattttcac ataaactaga
     4681 cgccttgtcg tcttcttctt cgtattcctt ctctttttca tttttctcct cataaaaatt
     4741 aacatagtta ttatcgtatc catatatgta tctatcgtat agagtaaatt ttttgttgtc
     4801 ataaatatat atgtcttttt taatggggtg tatagtaccg ctgcgcatag tttttctgta
     4861 atttacaaca gtgctatttt ctggtagttc ttcggagtgt gttgctttaa ttattaaatt
     4921 tatataatca atgaatttgg gatcgtcggt tttgtacaat atgttgccgg catagtacgc
     4981 agcttcttct agttcaatta caccattttt tagcagcacc ggattaacat aactttccaa
     5041 aatgttgtac gaaccgttaa acaaaaacag ttcacctccc ttttctatac tattgtctgc
     5101 gagcagttgt ttgttgttaa aaataacagc cattgtaatg agacgcacaa actaatatca
     5161 caaactggaa atgtctatca atatatagtt gctgatataa cttcgtatag catacattat
     5221 acgaagttat ctggttgaaa cgtacccgta gtggctatgg cagggcttgc cgccccgacg
     5281 ttggctgcga gccctgggcc ttcacccgaa cttgggggtt ggggtgggga aaaggaagaa
     5341 acgcgggcgt attggtccca atggggtctc ggtggggtat cgacagagtg ccagccctgg
     5401 gaccgaaccc cgcgtttatg aacaaacgac ccaacacccg tgcgttttat tctgtctttt
     5461 tattgccgtc atagcgcggg ttccttccgg tattgtctcc ttccgtgttt cagttagcct
     5521 cccccatctc ccgctattac tcgagtcact gggagccgga gtggcgggcc tcggcgtgct
     5581 cgtactgctc caccacggtg tagtcctcgt tgtggttggt gatgtccagc ttggagtcca
     5641 cgtagtggtt gccgggcagc tgcacgggct tcttggcctt gtacacggtc ttgaagtcgc
     5701 aggtgtagtg gccgccgtcc ttcagcttca gggcgtggga gatctcgccc ttcagcacgc
     5761 cgtcctgggg gtacagcttc tcggtggagg gctcccagcc ggcagtcttc ttctgcatta
     5821 cggggccgtc ggcggggaag ttcacgccct tgaacttcac cttgtagatg aaggtgccgt
     5881 cctgcaggga ggagtcctgc tgcacctcca ccacgccgcc gtcctcgaag ttcatggagc
     5941 gctcccaggt gaagccctcg gggaaggaca gcttcatgta gtcggggatg tcggcggggt
     6001 gcttcacgta ggccttggag ccgtactgga actgggggga caggatgtcc caggcgaagg
     6061 gcagggggcc gcccttggtc acctgcagct tggcggtctg ggtgccctcg tagggcttgc
     6121 cctcgccctc gccctcgatc tcgaagtagt ggccgttcac ggagccctcc atgcgcacct
     6181 tgaactgcat gaactccttg atgacgtcct cggtgttgtc catggtagag tgattgtaaa
     6241 taaaatgtaa tttacagtat agtattttaa ttaatataca aatgatttga taataattct
     6301 tatttaacta taatatattg tgttgggttg aattaaaggt ccgtatacta gtatcgattc
     6361 gcgacctact ccggaatatt aatagagttg ctgatatcat ggagataatt aaaatgataa
     6421 ccatctcgca aataaataag tattttactg ttttcgtaac agttttgtaa taaaaaaacc
     6481 tataaatacg gatctaccat gggcagcagc catcatcatc atcatcactc cgcggcccac
     6541 atagttatgg tagatgcgta caagcctact aaaggcggcc gcatgtcgta caactacgtg
     6601 gtaacggccc agaagcccac cgccgtgaac ggctgcgtga ccggacactt tacttcggcc
     6661 gaagacttaa acctgttgat tgccaaaaac acgagattag agatctatgt ggtcaccgcc
     6721 gaggggcttc ggcccgtcaa agaggtgggc atgtatggga agattgcggt catggagctt
     6781 ttcaggccca agggggagag caaggacctg ctgtttatct tgacagcgaa gtacaatgcc
     6841 tgcatcctgg agtataaaca gagtggcgag agcattgaca tcattacgcg agcccatggc
     6901 aatgtccagg accgcattgg ccgcccctca gagaccggca ttattggcat cattgaccct
     6961 gagtgccgga tgattggcct gcgtctctat gatggccttt tcaaggttat tccactagat
     7021 cgcgataata aagaactcaa ggccttcaac atccgcctgg aggagctgca tgtcattgat
     7081 gtcaagttcc tatatggttg ccaagcacct actatttgct ttgtctacca ggaccctcag
     7141 gggcggcacg taaaaaccta tgaggtgtct ctccgagaaa aggaattcaa taagggccct
     7201 tggaaacagg aaaatgtcga agctgaagct tccatggtga tcgcagtccc agagcccttt
     7261 gggggggcca tcatcattgg acaggagtca atcacctatc acaatggtga caaatacctg
     7321 gctattgccc ctcctatcat caagcaaagc acgattgtgt gccacaatcg agtggaccct
     7381 aatggctcaa gatacctgct gggagacatg gaaggccggc tcttcatgct gcttttggag
     7441 aaggaggaac agatggatgg caccgtcact ctcaaggatc tccgtgtaga actccttgga
     7501 gagacctcta ttgctgagtg cttgacatac cttgataatg gtgttgtgtt tgtcgggtct
     7561 cgcctgggtg actcccagct tgtgaagctc aacgttgaca gtaatgaaca aggctcctat
     7621 gtagtggcca tggaaacctt taccaactta ggacccattg tcgatatgtg cgtggtggac
     7681 ctggagaggc aggggcaggg gcagctggtc acttgctctg gggctttcaa ggaaggttct
     7741 ttgcggatca tccggaatgg aattggagga aatggcaact cgggcgagat ccagaagctg
     7801 cacattcgca cagttcccct ctatgagtct ccaaggaaga tctgctacca ggaagtgtcc
     7861 cagtgtttcg gggtcctctc cagccgcatt gaagtccaag acacgagtgg gggcacgaca
     7921 gccttgaggc ccagcgctag cacccaggct ctgtccagca gtgtaagctc cagcaagctg
     7981 ttctccagca gcactgctcc tcatgagacc tcctttggag aagaggtgga ggtgcacaac
     8041 ctacttatca ttgaccaaca cacctttgaa gtgcttcatg cccaccagtt tctgcagaat
     8101 gaatatgccc tcagtctggt ttcctgcaag ctgggcaaag accccaacac ttacttcatt
     8161 gtgggcacag caatggtgta tcctgaagag gcagagccca agcagggtcg cattgtggtc
     8221 tttcagtatt cggatggaaa actacagact gtggctgaaa aggaagtgaa aggggccgtg
     8281 tactctatgg tggaatttaa cgggaagctg ttagccagca tcaatagcac ggtgcggctc
     8341 tatgagtgga caacagagaa ggagctgcgc actgagtgca accactacaa caacatcatg
     8401 gccctctacc tgaagaccaa gggcgacttc atcctggtgg gcgaccttat gcgctcagtg
     8461 ctgctgcttg cctacaagcc catggaagga aactttgaag agattgctcg agactttaat
     8521 cccaactgga tgagtgctgt ggaaatcttg gatgatgaca attttctggg ggctgaaaat
     8581 gcctttaact tgtttgtgtg tcaaaaggat agcgctgcca ccactgacga ggagcggcag
     8641 cacctccagg aggttggtct tttccacctg ggcgagtttg tcaatgtctt ttgccacggc
     8701 tctctggtaa tgcagaatct gggtgagact tccaccccca cacaaggctc ggtgctcttc
     8761 ggcacggtca acggcatgat agggctggtg acctcactgt cagagagctg gtacaacctc
     8821 ctgctggaca tgcagaatcg actcaataaa gtcatcaaaa gtgtggggaa gatcgagcac
     8881 tccttctgga gatcctttca caccgagcgg aagacagaac cagccacagg tttcatcgac
     8941 ggtgacttga ttgagagttt cctggatatt agccgcccca agatgcagga ggtggtggca
     9001 aacctacagt atgacgatgg cagcggtatg aagcgagagg ccactgcaga cgacctcatc
     9061 aaggttgtgg aggagctaac tcggatccat tagggtaccc ccggacggcc gcttaattaa
     9121 ttgatccggg ttattagtac atttattaag cgctagattc tgtgcgttgt tgatttacag
     9181 acaattgttg tacgtatttt aataattcat taaatttata atctttaggg tggtatgtta
     9241 gagcgaaaat caaatgattt tcagcgtctt tatatctgaa tttaaatatt aa
//
