LOCUS       sequence-240879-       10791 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of BRAF A404Cfs*9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10791)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 10791)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-BRAF-A404Cfs*9
FEATURES             Location/Qualifiers
     source          1..10791
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2223..3401
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2454..3392
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3349..3369
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    3421..3445
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      3446..3455
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(5751..5775)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        5783..5801
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5826..5845)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5827..5845)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(5851..5871)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             5925..5966
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    5987..6554
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    6004..6556
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6148..6165)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6375..6394
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6568..7167
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6568..6587)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7064..7084
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7181..7769
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7234..7254)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7652..7663)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7844..8077
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(8196..8214)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8282..8386
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8387..8455,8456..9247)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8605..8624)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9418..10006
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9907..9926
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10160..10177
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10417..10438
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10453..10458,10459..10476,10477..10483)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10491..10507
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10496..10518
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10515..10531
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10515..10531
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10581..10600
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccct gcagatatca
     3421 acaagtttgt acaaaaaagc aggcaccatg gcggcgctga gcggtggcgg tggtggcggc
     3481 gcggagccgg gccaggctct gttcaacggg gacatggagc ccgaggccgg cgccggcgcc
     3541 ggcgccgcgg cctcttcggc tgcggaccct gccattccgg aggaggtgtg gaatatcaaa
     3601 caaatgatta agttgacaca ggaacatata gaggccctat tggacaaatt tggtggggag
     3661 cataatccac catcaatata tctggaggcc tatgaagaat acaccagcaa gctagatgca
     3721 ctccaacaaa gagaacaaca gttattggaa tctctgggga acggaactga tttttctgtt
     3781 tctagctctg catcaatgga taccgttaca tcttcttcct cttctagcct ttcagtgcta
     3841 ccttcatctc tttcagtttt tcaaaatccc acagatgtgg cacggagcaa ccccaagtca
     3901 ccacaaaaac ctatcgttag agtcttcctg cccaacaaac agaggacagt ggtacctgca
     3961 aggtgtggag ttacagtccg agacagtcta aagaaagcac tgatgatgag aggtctaatc
     4021 ccagagtgct gtgctgttta cagaattcag gatggagaga agaaaccaat tggttgggac
     4081 actgatattt cctggcttac tggagaagaa ttgcatgtgg aagtgttgga gaatgttcca
     4141 cttacaacac acaactttgt acgaaaaacg tttttcacct tagcattttg tgacttttgt
     4201 cgaaagctgc ttttccaggg tttccgctgt caaacatgtg gttataaatt tcaccagcgt
     4261 tgtagtacag aagttccact gatgtgtgtt aattatgacc aacttgattt gctgtttgtc
     4321 tccaagttct ttgaacacca cccaatacca caggaagagg cgtccttagc agagactgcc
     4381 ctaacatctg gatcatcccc ttccgcaccc gcctcggact ctattgggcc ccaaattctc
     4441 accagtccgt ctccttcaaa atccattcca attccacagc ccttccgacc agcagatgaa
     4501 gatcatcgaa atcaatttgg gcaacgagac cgatcctcat cagctcccaa tgtgcatata
     4561 aacacaatag aacctgtcaa tattgatgac ttgattagag accaaggatt tcgtggtgat
     4621 ggaggatcaa ccacaggttt gtctgctacc cccccctgcc tcattacctg gctcactaac
     4681 taacgtgaaa gccttacaga aatctccagg acctcagcga gaaaggaagt catcttcatc
     4741 ctcagaagac aggaatcgaa tgaaaacact tggtagacgg gactcgagtg atgattggga
     4801 gattcctgat gggcagatta cagtgggaca aagaattgga tctggatcat ttggaacagt
     4861 ctacaaggga aagtggcatg gtgatgtggc agtgaaaatg ttgaatgtga cagcacctac
     4921 acctcagcag ttacaagcct tcaaaaatga agtaggagta ctcaggaaaa cacgacatgt
     4981 gaatatccta ctcttcatgg gctattccac aaagccacaa ctggctattg ttacccagtg
     5041 gtgtgagggc tccagcttgt atcaccatct ccatatcatt gagaccaaat ttgagatgat
     5101 caaacttata gatattgcac gacagactgc acagggcatg gattacttac acgccaagtc
     5161 aatcatccac agagacctca agagtaataa tatatttctt catgaagacc tcacagtaaa
     5221 aataggtgat tttggtctag ctacagtgaa atctcgatgg agtgggtccc atcagtttga
     5281 acagttgtct ggatccattt tgtggatggc accagaagtc atcagaatgc aagataaaaa
     5341 tccatacagc tttcagtcag atgtatatgc atttggaatt gttctgtatg aattgatgac
     5401 tggacagtta ccttattcaa acatcaacaa cagggaccag ataattttta tggtgggacg
     5461 aggatacctg tctccagatc tcagtaaggt acggagtaac tgtccaaaag ccatgaagag
     5521 attaatggca gagtgcctca aaaagaaaag agatgagaga ccactctttc cccaaattct
     5581 cgcctctatt gagctgctgg cccgctcatt gccaaaaatt caccgcagtg catcagaacc
     5641 ctccttgaat cgggctggtt tccaaacaga ggattttagt ctatatgctt gtgcttctcc
     5701 aaaaacaccc atccaggcag ggggatatgg tgcgtttcct gtccactaga acccagcttt
     5761 cttgtacaaa gtggtgatat ccaattaacc ctcactaaag ggtaagatta gaagttaatg
     5821 aagaacccta tagtgagtcg tattaccacc caacttttct atacaaagtg gttgatatcc
     5881 agcacagtgg cggccgctcg agtctagagg gcccgcggtt cgaaggtaag cctatcccta
     5941 accctctcct cggtctcgat tctacgcgta ccggttagta atgagatccc tccccccccc
     6001 ctaacgttac tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc tatatgttat
     6061 tttccaccat attgccgtct tttggcaatg tgagggcccg gaaacctggc cctgtcttct
     6121 tgacgagcat tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg
     6181 tcgtgaagga agcagttcct ctggaagctt cttgaagaca aacaacgtct gtagcgaccc
     6241 tttgcaggca gcggaacccc ccacctggcg acaggtgcct ctgcggccaa aagccacgtg
     6301 tataagatac acctgcaaag gcggcacaac cccagtgcca cgttgtgagt tggatagttg
     6361 tggaaagagt caaatggctc tcctcaagcg tattcaacaa ggggctgaag gatgcccaga
     6421 aggtacccca ttgtatggga tctgatctgg ggcctcggtg cacatgcttt acatgtgttt
     6481 agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa
     6541 aaacacgatg ataatatggc cacacatatg accgagtaca agcccacggt gcgcctcgcc
     6601 acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc
     6661 gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa
     6721 ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc
     6781 gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc
     6841 ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc
     6901 ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg
     6961 cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc
     7021 gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac
     7081 gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg
     7141 tgcatgaccc gcaagcccgg tgcctaaatc gatagatcct aatcaacctc tggattacaa
     7201 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
     7261 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc
     7321 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg
     7381 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac
     7441 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat
     7501 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt
     7561 ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat
     7621 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc
     7681 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag
     7741 tcggatctcc ctttgggccg cctccccgcc tgagatcctt taagaccaat gacttacaag
     7801 gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac
     7861 tcccaacgaa gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc
     7921 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     7981 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     8041 ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt
     8101 attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggcc cgggttaatt
     8161 aaggaaaggg ctagatcatt cttgaagacg aaagggcctc gtgatacgcc tatttttata
     8221 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
     8281 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     8341 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     8401 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     8461 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     8521 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     8581 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg
     8641 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     8701 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     8761 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     8821 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     8881 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     8941 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     9001 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     9061 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     9121 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     9181 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     9241 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     9301 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     9361 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     9421 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     9481 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     9541 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     9601 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     9661 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     9721 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     9781 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     9841 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     9901 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     9961 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
    10021 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
    10081 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
    10141 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
    10201 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agcaagctca tggctgacta
    10261 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
    10321 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tccccgtggc acgacaggtt
    10381 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
    10441 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
    10501 ataacaattt cacacaggaa acagctatga catgattacg aatttcacaa ataaagcatt
    10561 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
    10621 gatcaactgg ataactcaag ctaaccaaaa tcatcccaaa cttcccaccc cataccctat
    10681 taccactgcc aattacctgt ggtttcattt actctaaacc tgtgattcct ctgaattatt
    10741 ttcattttaa agaaattgta tttgttaaat atgtactaca aacttagtag t
//
