LOCUS       sequence-240881-       10638 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Ezh2 from the TRE3G promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10638)
  AUTHORS   Liu Y, Yu C, Daley TP, Wang F, Cao WS, Bhate S, Lin X, Still C 2nd, 
            Liu H, Zhao D, Wang H, Xie XS, Ding S, Wong WH, Wernig M, Qi LS
  TITLE     CRISPR Activation Screens Systematically Identify Factors that Drive
            Neuronal Fate and Reprogramming.
  JOURNAL   Cell Stem Cell. 2018 Sep 28. pii: S1934-5909(18)30440-5. doi: 
            10.1016/j.stem.2018.09.003.
  PUBMED    30318302
REFERENCE   2  (bases 1 to 10638)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2018 Sep 28. pii: S1934-5909(18)30440-5. doi: 
            10.1016/j.stem.2018.09.003."
COMMENT     Sequence Label: TetO-FUW-Ezh2
FEATURES             Location/Qualifiers
     source          1..10638
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        388..586
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             604..784
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    831..956
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1449..1682
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1867..1911
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2209..2326
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(2383..2401,2402..2424,2425..2443,2444..2466,2467..2485,
                     2486..2508,2509..2527,2528..2550,2551..2569,2570..2592,
                     2593..2611,2612..2634,2635..2653)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2686..2724
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2721..2745
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2828..2837
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    5099..5687
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5152..5172)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5570..5581)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5690..5706)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5691..5707)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6212..6392
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6418..6435)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6424..6648
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6694..7122
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6781..6800)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6991..7012
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7131..7151)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7136..7465
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7316..7451
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7378..7397
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7513..7560
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7579..7953
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8083..8204
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8120..8139)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8174..8193
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8253..8269)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8253..8269)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8266..8288)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8277..8293
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8301..8307,8308..8325,8326..8331))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8346..8367
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8484..8501)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8655..9243)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8735..8754)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9414..10205,10206..10274))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10037..10056
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10275..10379)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10454..10473)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagcgcg ttttgcctgt
      601 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
      661 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
      721 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
      781 agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac
      841 gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta
      901 cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta
      961 ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga
     1021 aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta
     1081 atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat
     1141 cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca accctctatt
     1201 gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag
     1261 agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga
     1321 ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa
     1381 ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca
     1441 gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca
     1501 gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag
     1561 aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc
     1621 atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc
     1681 ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct
     1741 agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac
     1801 agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag
     1861 caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg
     1921 tttaacataa caaattggct gtggtatata aaattattca taatgatagt aggaggcttg
     1981 gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat
     2041 tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga
     2101 atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatcg
     2161 gcactgcgtg cgccaattct gcagacaaat ggcagtattc atccacaatt ttaaaagaaa
     2221 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat
     2281 acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag
     2341 ggacagcaga gatccagttt ggttagatct cgagtttacc actccctatc agtgatagag
     2401 aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt
     2461 taccactccc tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg
     2521 atagagaaaa gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt
     2581 cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta
     2641 tcagtgatag agaaaagtga aagtcgagct cggtacccgg gtcgaggtag gcgtgtacgg
     2701 tgggaggcct atataagcag agctcgttta gtgaaccgtc agatcgcctg gagacgccat
     2761 ccacgctgtt ttgacctcca tagaagacac cgggaccgat ccagcctccg cggccccgaa
     2821 ttcgctagcc accatgggcc agactgggaa gaaatctgag aagggaccgg tttgttggcg
     2881 gaagcgtgta aaatcagagt acatgagact gagacagctc aagaggttca gaagagctga
     2941 tgaagtaaag actatgttta gttccaatcg tcagaaaatt ttggaaagaa ctgaaacctt
     3001 aaaccaagag tggaagcagc ggaggataca gcctgtgcac atcatgactt ctgtgagctc
     3061 attgcgcggg actagggagt gttcagtcac cagtgacttg gattttccag cacaagtcat
     3121 cccgttaaag accctgaatg cagtcgcctc ggtgcctata atgtactctt ggtcgccctt
     3181 acaacagaat tttatggtgg aagacgaaac tgtcttacat aacattcctt acatggggga
     3241 tgaagttctg gatcaggacg gcactttcat tgaagaacta ataaaaaatt atgatggaaa
     3301 agtgcatggt gacagagaat gtggatttat aaatgatgaa atttttgtgg agttggtaaa
     3361 tgctcttggt caatataatg atgatgatga tgacgatgat ggagatgatc cagatgaaag
     3421 agaagaaaaa cagaaagatc tagaggataa tcgagatgat aaagaaactt gcccacctcg
     3481 gaaatttcct gctgataaaa tatttgaagc catttcctca atgtttccag ataagggcac
     3541 cgcagaagaa ctgaaggaaa aatataaaga actcacggag cagcagctcc caggtgctct
     3601 gcctcctgaa tgtactccaa acatcgatgg accaaatgcc aaatctgttc agagggagca
     3661 aagcttgcat tcatttcata cgctcttctg tcgacgatgt tttaagtatg actgcttcct
     3721 acatcccttc catgcaacac ccaacacata taagaggaag aacacagaaa cagctttgga
     3781 caacaagcct tgtggaccac agtgttacca gcatctggag ggagctaagg agtttgctgc
     3841 tgctcttact gctgagcgga taaagacacc acctaaacgc ccagggggcc gcagaagagg
     3901 aagacttccg aataacagta gcagacccag cacccccacc atcagtgtgc tggagtcaaa
     3961 ggatacagac agtgacagag aagcagggac tgaaactggg ggagagaaca acgataaaga
     4021 agaagaagag aaaaaagatg agacgtccag ctcctctgaa gcaaattctc ggtgtcaaac
     4081 accaataaag atgaagccaa atattgaacc tcctgagaat gtggagtgga gtggtgctga
     4141 agcctccatg tttagagtcc tcattggtac ttactacgat aacttttgtg ccattgctag
     4201 gctaattggg accaaaacat gtagacaggt gtatgagttt agagtcaagg agtccagtat
     4261 catagcacct gttcccactg aggatgtaga cactcctcca agaaagaaga aaaggaaaca
     4321 tcggttgtgg gctgcacact gcagaaagat acaactgaaa aaggacggct cctctaacca
     4381 tgtttacaac tatcaaccct gtgaccatcc acggcagcct tgtgacagtt cgtgcccttg
     4441 tgtgatagca caaaattttt gtgaaaagtt ttgtcaatgt agttcagagt gtcaaaaccg
     4501 ctttcctgga tgtcggtgca aagcacaatg caacaccaaa cagtgtccat gctacctggc
     4561 tgtccgagag tgtgaccctg acctctgtct cacgtgtgga gctgctgacc attgggacag
     4621 taaaaatgta tcctgtaaga actgtagcat tcagcggggc tctaaaaagc acttactgct
     4681 ggcaccgtct gatgtggcag gctggggcat ctttatcaaa gatcctgtac aaaaaaatga
     4741 attcatctca gaatactgtg gggagattat ttctcaggat gaagcagaca gaagaggaaa
     4801 agtgtatgac aaatacatgt gcagctttct gttcaacttg aacaatgatt ttgtggtgga
     4861 tgcaacccga aagggcaaca aaattcgttt tgctaatcat tcagtaaatc caaactgcta
     4921 tgcaaaagtt atgatggtta atggtgacca caggataggc atctttgcta agagggctat
     4981 ccagactggt gaagagttgt tttttgatta cagatacagc caggctgatg ccctgaagta
     5041 tgtgggcatc gaacgagaaa tggaaatccc ttaggaattc gatatcaagc ttatcgataa
     5101 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
     5161 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
     5221 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
     5281 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
     5341 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
     5401 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
     5461 gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc
     5521 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
     5581 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
     5641 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcatc gataccgtcg
     5701 acctcgagac ctagaaaaac atggagcaat cacaagtagc aatacagcag ctaccaatgc
     5761 tgattgtgcc tggctagaag cacaagagga ggaggaggtg ggttttccag tcacacctca
     5821 ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga
     5881 aaagggggga ctggaagggc taattcactc ccaacgaaga caagatatcc ttgatctgtg
     5941 gatctaccac acacaaggct acttccctga ttggcagaac tacacaccag ggccagggat
     6001 cagatatcca ctgacctttg gatggtgcta caagctagta ccagttgagc aagagaaggt
     6061 agaagaagcc aatgaaggag agaacacccg cttgttacac cctgtgagcc tgcatgggat
     6121 ggatgacccg gagagagaag tattagagtg gaggtttgac agccgcctag catttcatca
     6181 catggcccga gagctgcatc cggactgtac tgggtctctc tggttagacc agatctgagc
     6241 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     6301 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     6361 acccttttag tcagtgtgga aaatctctag cagggcccgt ttaaacccgc tgatcagcct
     6421 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga
     6481 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt
     6541 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg
     6601 attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg
     6661 aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg
     6721 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
     6781 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     6841 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     6901 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     6961 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     7021 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
     7081 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg
     7141 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     7201 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     7261 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
     7321 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     7381 ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt
     7441 ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc
     7501 tgatcagcac gtgttgacaa ttaatcatcg gcatagtata tcggcatagt ataatacgac
     7561 aaggtgagga actaaaccat ggccaagttg accagtgccg ttccggtgct caccgcgcgc
     7621 gacgtcgccg gagcggtcga gttctggacc gaccggctcg ggttctcccg ggacttcgtg
     7681 gaggacgact tcgccggtgt ggtccgggac gacgtgaccc tgttcatcag cgcggtccag
     7741 gaccaggtgg tgccggacaa caccctggcc tgggtgtggg tgcgcggcct ggacgagctg
     7801 tacgccgagt ggtcggaggt cgtgtccacg aacttccggg acgcctccgg gccggccatg
     7861 accgagatcg gcgagcagcc gtgggggcgg gagttcgccc tgcgcgaccc ggccggcaac
     7921 tgcgtgcact tcgtggccga ggagcaggac tgacacgtgc tacgagattt cgattccacc
     7981 gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc
     8041 ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct
     8101 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     8161 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg
     8221 tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     8281 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     8341 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     8401 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     8461 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     8521 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     8581 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     8641 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     8701 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     8761 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     8821 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     8881 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     8941 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     9001 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     9061 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     9121 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     9181 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     9241 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     9301 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     9361 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     9421 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     9481 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     9541 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     9601 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     9661 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     9721 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     9781 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     9841 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     9901 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     9961 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
    10021 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
    10081 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
    10141 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
    10201 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
    10261 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
    10321 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
    10381 acatttcccc gaaaagtgcc acctgacgtc gacggatcgg gagatctccc gatcccctat
    10441 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat ctgctccctg
    10501 cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag
    10561 gcttgaccga caattgcatg aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg
    10621 atgtacgggc cagatata
//
