LOCUS       sequence-240884-        1100 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  KO control .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1100)
  AUTHORS   Zhang Y, Shi J, Liu X, Feng L, Gong Z, Koppula P, Sirohi K, Li X, 
            Wei Y, Lee H, Zhuang L, Chen G, Xiao ZD, Hung MC, Chen J, Huang P, 
            Li W, Gan B
  TITLE     BAP1 links metabolic regulation of ferroptosis to tumour 
            suppression.
  JOURNAL   Nat Cell Biol. 2018 Oct;20(10):1181-1192. doi: 
            10.1038/s41556-018-0178-0. Epub 2018 Sep 10.
  PUBMED    30202049
REFERENCE   2  (bases 1 to 1100)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41556-018-0178-0"; journalName: "Nat Cell Biol"; date: 
            "2018-10"; volume: "20"; issue: "10"; pages: "1181-1192"
COMMENT     Sequence Label: lentiCRISPR v2-sgControl
FEATURES             Location/Qualifiers
     source          1..1100
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     127..146
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        226..301
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        363..574
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      593..602
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 aggactgtta gagagataat tagaattaat ttgactgtaa acacaaagat attagtacaa
       61 aatacgtgac gtagaaagta ataatttctt gggtagtttg cagttttaaa attatgtttt
      121 aaaatggact atcatatgct taccgtaact tgaaagtatt tcgatttctt ggctttatat
      181 atcttgtgga aaggacgaaa caccggcact accagagcta actcagtttt agagctagaa
      241 atagcaagtt aaaataaggc tagtccgtta tcaacttgaa aaagtggcac cgagtcggtg
      301 cttttttgaa ttcgctagct aggtcttgaa aggagtggga attggctccg gtgcccgtca
      361 gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg
      421 atccggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct
      481 ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt
      541 tctttttcgc aacgggtttg ccgccagaac acaggaccgg ttctagagcg ctgccaccat
      601 ggacaagaag tacagcatcg gcctggacat cggcaccaac tctgtgggct gggccgtgat
      661 caccgacgag tacaaggtgc ccagcaagaa attcaaggtg ctgggcaaca ccgaccggca
      721 cagcatcaag aagaacctga tcggagccct gctgttcgac agcggcgaaa cagccgaggc
      781 cacccggctg aagagaaccg ccagaagaag atacaccaga cggaagaacc ggatctgcta
      841 tctgcaagag atcttcagca acgagatggc caagtggacg acagcttctt ccacagactg
      901 gaagagtcct tcctggtgga agaggataga agcacgagcg gcaccccatc tcggcaacat
      961 cgtgacgagt gctacacgag agtaccccac atctacactg agaagaactg tgacagcacg
     1021 acagcgactg cgctgatcta tctgcctgcc acatgatcag tcgggcactc tgatcgagcg
     1081 actgacccga caacgcgact
//
