LOCUS       sequence-240885-        1100 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  KO BAP1 gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1100)
  AUTHORS   Zhang Y, Shi J, Liu X, Feng L, Gong Z, Koppula P, Sirohi K, Li X, 
            Wei Y, Lee H, Zhuang L, Chen G, Xiao ZD, Hung MC, Chen J, Huang P, 
            Li W, Gan B
  TITLE     BAP1 links metabolic regulation of ferroptosis to tumour 
            suppression.
  JOURNAL   Nat Cell Biol. 2018 Oct;20(10):1181-1192. doi: 
            10.1038/s41556-018-0178-0. Epub 2018 Sep 10.
  PUBMED    30202049
REFERENCE   2  (bases 1 to 1100)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41556-018-0178-0"; journalName: "Nat Cell Biol"; date: 
            "2018-10"; volume: "20"; issue: "10"; pages: "1181-1192"
COMMENT     Sequence Label: lentiCRISPR v2-sgBAP1-1
FEATURES             Location/Qualifiers
     source          1..1100
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     131..150
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        230..305
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        367..578
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      597..606
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1085..1096
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 tacaaaggct gttagagaga taattagaat taatttgact gtaaacacaa agatattagt
       61 acaaaatacg tgacgtagaa agtaataatt tcttgggtag tttgcagttt taaaattatg
      121 ttttaaaatg gactatcata tgcttaccgt aacttgaaag tatttcgatt tcttggcttt
      181 atatatcttg tggaaaggac gaaacaccga cgagcagggt gaagaggccg ttttagagct
      241 agaaatagca agttaaaata aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc
      301 ggtgcttttt tgaattcgct agctaggtct tgaaaggagt gggaattggc tccggtgccc
      361 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
      421 attgatccgg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
      481 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
      541 acgttctttt tcgcaacggg tttgccgcca gaacacagga ccggttctag agcgctgcca
      601 ccatggacaa gaagtacagc atcggcctgg acatcggcac caactctgtg ggctgggccg
      661 tgatcaccga cgagtacaag gtgcccagca agaaattcaa ggtgctgggc aacaccgacc
      721 ggcacagcat caagaagaac ctgatcggag ccctgctgtt cgacagcggc gaaacagccg
      781 aggccacccg gctgaagaga accgccagaa gaagatacac cagacggaag aaccggatct
      841 gctatctgca agagatcttc agcaacgaga tggccaagtg gacgacagct tcttccacag
      901 actggaagag tccttcctgg tggaagagga taagaagcac gagcggcacc ccatcttcgg
      961 caacatcgtg gacgagtgct accacgagag taccccacat ctacactgag aaagaactgt
     1021 gacagcacga cagcgactgc gctgatctat tctgccctgc cacatgatca gtcgggccac
     1081 tctgatcgag gggcgactga
//
