LOCUS       sequence-240886-        1100 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  KO BAP1 gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1100)
  AUTHORS   Zhang Y, Shi J, Liu X, Feng L, Gong Z, Koppula P, Sirohi K, Li X, 
            Wei Y, Lee H, Zhuang L, Chen G, Xiao ZD, Hung MC, Chen J, Huang P, 
            Li W, Gan B
  TITLE     BAP1 links metabolic regulation of ferroptosis to tumour 
            suppression.
  JOURNAL   Nat Cell Biol. 2018 Oct;20(10):1181-1192. doi: 
            10.1038/s41556-018-0178-0. Epub 2018 Sep 10.
  PUBMED    30202049
REFERENCE   2  (bases 1 to 1100)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41556-018-0178-0"; journalName: "Nat Cell Biol"; date: 
            "2018-10"; volume: "20"; issue: "10"; pages: "1181-1192"
COMMENT     Sequence Label: lentiCRISPR v2-sgBAP1-2
FEATURES             Location/Qualifiers
     source          1..1100
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     130..149
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        229..304
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        366..577
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      596..605
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 aaaaccgctg ttagagagat aattagaatt aatttgactg taaacacaaa gatattagta
       61 caaaatacgt gacgtagaaa gtaataattt cttgggtagt ttgcagtttt aaaattatgt
      121 tttaaaatgg actatcatat gcttaccgta acttgaaagt atttcgattt cttggcttta
      181 tatatcttgt ggaaaggacg aaacaccgta ccgaaatctt ccacgagcgt tttagagcta
      241 gaaatagcaa gttaaaataa ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg
      301 gtgctttttt gaattcgcta gctaggtctt gaaaggagtg ggaattggct ccggtgcccg
      361 tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag gggtcggcaa
      421 ttgatccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg
      481 gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa
      541 cgttcttttt cgcaacgggt ttgccgccag aacacaggac cggttctaga gcgctgccac
      601 catggacaag aagtacagca tcggcctgga catcggcacc aactctgtgg gctgggccgt
      661 gatcaccgac gagtacaagg tgcccagcaa gaaattcaag gtgctgggca acaccgaccg
      721 gcacagcatc aagaagaacc tgatcggagc cctgctgttc gacagcggcg aaacagccga
      781 ggccacccgg ctgaagagaa ccgccagaag aagatacacc agacggaaga accggatctg
      841 ctatctgcaa gagatcttca gcaacgagat ggccaagtgg acgacagctt cttccacaga
      901 ctggaagagt ccttcctggt ggaagaggat agaagcacga gcggcacccc atcttcggca
      961 catcgtggac gagtgcctac cacgagagta ccccaccatc tacactgaga agaactgtga
     1021 cagcacgaca gcgactgcgc tgatctatct gcctgccaca tgatcagttc ggggccactc
     1081 tgatcgagcg actgacccga
//
