LOCUS       sequence-24089-e        1105 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1105)
  TITLE     T4 Lysozyme Plasmids - Tray 2
REFERENCE   2  (bases 1 to 1105)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: T4 Lysozyme N40-[SLD]WT*
FEATURES             Location/Qualifiers
     source          1..1105
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    731..747
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(755..761,762..777,778..783))
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    826..842
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(850..856,857..872,873..878))
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     promoter        complement(join(962..968,969..986,987..992))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
ORIGIN
        1 ctangaaaag cccgctcatt aggcgggctc agatctgctc atgtttgaca gcttatcatc
       61 gataagcttc ggcgataaat tcttgatatg ttttcatatg tttcctttaa atattttaat
      121 tagtaattgt ctattcaagt aattgtgaat atactatcac aattccaaga gaaagtaaac
      181 agctttatag atttttatac gcgtcccaag tgccagttct aaacgttgta atgactcgtt
      241 ttgcgcgatt aggtgtttga ttataccatc tacttttagc taagttaact gctgcttcat
      301 cccagcgttt ttgttgaagc atacgtaaag agttagtaaa tcctgccaca ccggtttctc
      361 ccatttggaa aaccatatta atcaatgcag cgcgacgaac cgcatcaaga gaatcataaa
      421 ccggttttaa tttagcattt ctcagaattc cgcgaacagc agcatcaaca tcctgattaa
      481 agagtttttc agcctcatct tttgtaatta caccattagt attacgccca atagctttat
      541 ctaattcaga tttagcagcg tccaagctat taagtgatgg actttttgta agcaaatgac
      601 cgatgccaat agtgtaatag ccttctgtgt ctttatagat tttaagtcta agaccttcat
      661 ctatacgtaa catttcaaat atattcataa tacctcctaa gcatcgatgg atcctgtttc
      721 ctgtgtgaaa ttgttatccg ctcacaattc cacacattat acgagccgat gattaattgt
      781 caacaggggg atggggagta agctgatcct gtttcctgtg tgaaattgtt atccgctcac
      841 aattccacac attatacgag ccgatgatta attgtcaaca gggggatggg gagtaagctc
      901 atcgatggat cgacccgggg nttnccgggg gggaaantgg ttatccgctc acaattcccc
      961 acattatacg agccggaagc ataaagtgta aagccngggg ggncctaang agngagctaa
     1021 cttacnntta attgcgttgc nctcncngnc ccncttncca gtcgggaaac cnggncgggc
     1081 cggganccnn cnaagggggc aaanc
//
