LOCUS       sequence-24090-e        1087 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1087)
  TITLE     T4 Lysozyme Plasmids - Tray 2
REFERENCE   2  (bases 1 to 1087)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: T4 Lysozyme N40-[SLD] L46A WT*
FEATURES             Location/Qualifiers
     source          1..1087
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    733..749
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(757..763,764..779,780..785))
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    828..844
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(852..858,859..874,875..879))
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
ORIGIN
        1 ctangaaaaa agcccgctca ttaggcgggc tcagatctgc tcatgtttga cagcttatca
       61 tcgataagct tcggcgataa attcttgata tgttttcata tgtttccttt aaatatttta
      121 attagtaatt gtctattcaa gtaattgtga atatactatc acaattccaa gagaaagtaa
      181 acagctttat agatttttat acgcgtccca agtgccagtt ctaaacgttg taatgactcg
      241 ttttgcgcga ttaggtgttt gattatacca tctactttta gctaagttaa ctgctgcttc
      301 atcccagcgt ttttgttgaa gcatacgtaa agagttagta aatcctgcca caccggtttc
      361 tcccatttgg aaaaccatat taatcaatgc agcgcgacga accgcatcaa gagaatcata
      421 aaccggtttt aatttagcat ttctcagaat tccgcgaaca gcagcatcaa catcctgatt
      481 aaagagtttt tcagcctcat cttttgtaat tacaccatta gtattacgcc caatagcttt
      541 atctgcttca gatttagcag cgtccaagct attaagtgat ggactttttg taagcaaatg
      601 accgatgcca atagtgtaat agccttctgt gtctttatag attttaagtc taagaccttc
      661 atctatacgt aacatttcaa atatattcat aatacctcct aagcatcgat ggatcctgtt
      721 tcctgtgtga aattgttatc cgctcacaat tccacacatt atacgagccg atgattaatt
      781 gtcaacaggg ggatggggag taagctgatc ctgtttcctg tgtgaaattg ttatccgctc
      841 acaattccac acattatacg agccgatgat taattgtcan cagggggatg gggagtaagc
      901 tcatcgatgg atcgaccggg nttnccgggg ggaaatggta anccgcncnc aatncnnnca
      961 ttanacgagc cggaagcana aagngtaagc cggggngcct angagngact acttacataa
     1021 ttgcgtggcg cnccggccnc ttncngtcgg gaacctgncg gncnggacnc ntcnangggg
     1081 naaacct
//
