LOCUS       sequence-240905-        6027 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BglFusion compatible IPTG inducible integration vector for 
            Streptococcus pneumoniae (pPEPZ Backbone).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6027)
  AUTHORS   Keller LE, Rueff AS, Kurushima J, Veening JW
  TITLE     Three New Integration Vectors and Fluorescent Proteins for Use in 
            the Opportunistic Human Pathogen Streptococcus pneumoniae.
  JOURNAL   Genes (Basel). 2019 May 22;10(5). pii: genes10050394. doi: 
            10.3390/genes10050394.
  PUBMED    31121970
REFERENCE   2  (bases 1 to 6027)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes 
            (Basel)."; date: "2019-05-22"; volume: "10(5). pii: genes10050394. 
            doi"; pages: " 10.3390/genes10050394"
COMMENT     Sequence Label: pASR103
FEATURES             Location/Qualifiers
     source          1..6027
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      13..84
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      100..127
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     protein_bind    224..243
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     rep_origin      1374..1962
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1863..1882
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2050..2121
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2122..2190,2191..2982)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2340..2359)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     mobile_element  5000..5767
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
ORIGIN
        1 ctagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc
       61 tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct
      121 ttctgcgttt atagaattca ctagtaattg tgaggcggat aacaattctc actcattcta
      181 cagtttattc ttgacattgc actgtccccc tggtataata actaattgtg agcgctcaca
      241 attaagatct aaataaataa ggaggaaaaa taatgccgga tccctccagt aactcgagaa
      301 aaaaaaaccg cgcccctgac agggcgcggt ttttttttcg taatttatta ctgaacaaga
      361 gggcaatcat atctggaata atatgcttaa aaagagaagg aaaattggtg caaattcatt
      421 ttcagactat cttcgtggaa gtattcatca aaatagacaa aaataaattt ggataaatcg
      481 aactcactat tcaggaggca tatgagcaat tcgaaaaaga aaagtgtcaa attgagccta
      541 taggagtaga agtgaaatag taagtcctgc atagtggatg agagaaaagt tctccttgaa
      601 gttttcctga actatcagtc gcatgtcaaa cgatatgtag ggtaatgtga gaggggatag
      661 cgagtagttt ttggttattt tatcaaaaaa cttatatttt attataccga atgataaaat
      721 ataataaaaa tgatagaata aggaaaaaac atgaatgtca aaaagataat gtcaattttt
      781 caatcctttt atgttgatgt cagtattgag gaactgactt tgactttacc aatcagtttt
      841 gtaaaaaggt ttgagtatac tcaaatgact tttcataagg aatcattttt attgattaaa
      901 gaaaagagaa gggggagttt gagttcattt gttactcagg ctcgcactat gggtgaaaaa
      961 gccaatatgg atgttgtttt ggtgttttcg aagttatcag acagtgaaaa aaagcaatta
     1021 cttcaagcta gagttccgtt tgtagacttt aagggaaacc tcttcttccc tccattggga
     1081 ctagtactca atgcgaatga tactgaagtc cctaaggaat taacacctag cgaacaatta
     1141 acgtggattg cctttttatt gacaaaaggt caaaaagtag tagatgttga tttgctttca
     1201 caagtcactg gacttccaaa ctcaacaatt tataggtgtt tgaggacttt taaagcttta
     1261 tattggttaa acaagcaaaa taagctttac acatatacgg tgtcaaagaa agaattattc
     1321 ttaaaatccg tgtcatgttg gtaccacccc gtagaaaaga tcaaaggatc ttcttgagat
     1381 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct ggtagcggtg
     1441 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     1501 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac
     1561 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     1621 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     1681 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     1741 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     1801 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     1861 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     1921 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     1981 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     2041 cgcggccgcc attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     2101 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     2161 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     2221 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     2281 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     2341 ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata
     2401 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     2461 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     2521 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     2581 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     2641 gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact
     2701 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     2761 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     2821 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     2881 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     2941 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     3001 tcatagcggc cgccaataaa ttgcttcctt gttttgtcat aattttttta tttaaaaaat
     3061 tatgacaaaa gtgtgctatt ctttttatga gaggtgtatg aatatgataa atgtatgtga
     3121 taaatgtatg tgatgttgga aaaagaataa aagaacttag aatatcttca aatcttactc
     3181 aagataagat tgctgaatat ttgtctttat atttgtcttt gaatcaaagc atgattgcca
     3241 aaatggaaaa aggtgaaagg aatatcacga atggatttaa gtaataaagc ttcaaatctt
     3301 agaaaaaagt tgggagctga tggtgaagcg ccgatagata tttttaaatt ggtacaaaag
     3361 atagaaaatt tgacgctggt attttatgga ctcggaaaga atattagcgg agtctgttat
     3421 aaaggaactc agttcagtct cattgcagtc aattcagaca tgtcattagg aaggtaaaga
     3481 ttttctttag cacatgaact gtatcatctt tattatgatg aggtgaagaa gagttcagtc
     3541 agtcttatct tgattggtga aggagatgaa actgaaagaa aagcggatca gcttgcttct
     3601 tattttttaa ttttcccatc ttcactgtat aggatggttg aggaaatcag agaaaatgcc
     3661 aatagaactc atcttgaagt agaagatatt ataaaattgg gtcagtttta tggtatcagt
     3721 cataaagcta tgttatatag attgaggaat gatggatacc ttgatgcaga agaaattaaa
     3781 aatatggata ttagtgttat agagacagct tcaagattag gctatgatac aagtttatat
     3841 cgtcctttgt cagaaagtaa aaaggaaatg gtattaggat aatatattaa ttcaactgaa
     3901 caacttttag aaaataacag aatttcgcaa gggaagtatg aggaactgtt actagatgct
     3961 ttcagatatg atattgtata tgggctagat gaagaggggg gagttgtcgt tgaaaagctt
     4021 ctagcaaaaa actggacgga gtcccgtcca gaaaagtaag cacttgttat tgcaataaaa
     4081 ttagcttatt tcctcccgtt aaataataga taactattaa aaatagacaa tacttgctca
     4141 taagtaacgg tacttaaatt gtttactttg gcgtgtttca ttgcttgatg aaactgattt
     4201 ttagtaaaca gttgacgata ttctcgattg acccattttg aaacaaagta cgtatatagc
     4261 ttccaatatt tatctggaac atctgtggta tggcgggtaa gttttattaa gacactgttt
     4321 acttttggtt taggatgaaa gcattccgct ggcagcttaa gcaattgctg aatcgagact
     4381 tgagtgtgca agagcaaccc tagtgttcgg tgaatatcca aggtacgctt gtagaatcct
     4441 tcttcaacaa tcagatagat gtcagacgca tggctttcaa aaaccacttt tttaataatt
     4501 tgtgtgctta aatggtaagg aatattccca acaattttat acctctgttt gttagggaat
     4561 tgaaactgta gaatatcttg gtgaattaaa gtgacacgaa tgttcagttt taatttttct
     4621 gacgataagt tgaatagatg actgtctaat tcaatagacg ttacctgttt acttatttta
     4681 gccagtttcg tcgttaaatg ccctttacct gttccaattt cgtaaacggt atcggtttct
     4741 tttaaattca attgttttat tatttggttg agtacttttt cactcgttaa aaagttttga
     4801 gaatatttta tatttttgtt cattaatttt cctcctacat gatttcacct cgttgattat
     4861 gttcatataa agtttatatt gatactcaat ttacttaccc tagattggac atatacttaa
     4921 attactgttc aataaagctg accgttagcg tttaagtaca tcctttcaca atttgtctac
     4981 agaacagtac tggctattag gtaatgactc caacttattg atagtgtttt atgttcagat
     5041 aatgcccgat gactttgtca tgcagctcca ccgattttga gaacgacagc gacttccgtc
     5101 ccagccgtgc caggtgctgc ctcagattca ggttatgccg ctcaattcgc tgcgtatatc
     5161 gcttgctgat tacgtgcagc tttcccttca ggcgggattc atacagcggc cagccatccg
     5221 tcatccatat caccacgtca aagggtgaca gcaggctcat aagacgcccc agcgtcgcca
     5281 tagtgcgttc accgaatacg tgcgcaacaa ccgtcttccg gagactgtca tacgcgtaaa
     5341 acagccagcg ctggcgcgat ttagccccga catagcccca ctgttcgtcc atttccgcgc
     5401 agacgatgac gtcactgccc ggctgtatgc gcgaggttac cgactgcggc ctgagttttt
     5461 taagtgacgt aaaatcgtgt tgaggccaac gcccataatg cgggctgttg cccggcatcc
     5521 aacgccattc atggccatat caatgatttt ctggtgcgta ccgggttgag aagcggtgta
     5581 agtgaactgc agttgccatg ttttacggca gtgagagcag agatagcgct gatgtccggc
     5641 ggtgcttttg ccgttacgca ccaccccgtc agtagctgaa caggagggac agctgataga
     5701 aacagaagcc actggagcac ctcaaaaaca ccatcataca ctaaatcagt aagttggcag
     5761 catcacctgg ctattatatc agccttctta ttcaatgtca agaataaact gttctatgta
     5821 aacctattca ttgttttgca tgcggcaaag cactcaaaag tttaccttat gggtgctttt
     5881 ttcgtgcttt tttgaaaaga caaaaaaaag aaccttgcca agcaagattc ttttgatagg
     5941 gtggtctccg atcgtgtact gcagaaaact agtcgcaaaa aaccccgctt cggcggggtt
     6001 ttttcgctct agacggtgat caacacg
//
