LOCUS       sequence-24091-e        1092 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1092)
  TITLE     T4 Lysozyme Plasmids - Tray 2
REFERENCE   2  (bases 1 to 1092)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: T4 Lysozyme N40L-[A] WT*
FEATURES             Location/Qualifiers
     source          1..1092
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    727..743
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(751..757,758..773,774..779))
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    823..839
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(847..853,854..869,870..875))
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
ORIGIN
        1 tangaaaaaa gcccgcctca ttaggcgggc tcagatctgc tcatgtttga cagcttatca
       61 tcgataagct tcggcaataa attcttgata tgttttcata tgtttccttt aaatatttta
      121 attagtaatt gtctattcaa gtaattgtga atatactatc acaattccaa gagaaagtaa
      181 acagctttat agatttttat acgcgtccca agtgccagtt ctaaacgttg taatgactcg
      241 ttttgcgcga ttaggtgttt gattatacca tctactttta gctaagttaa ctgctgcttc
      301 atcccagcgt ttttgttgaa gcatacgtaa agagttagta aatcctgcca caccggtttc
      361 tcccatttgg aaaaccatat taatcaatgc agcgcgacga accgcatcaa gagaatcata
      421 aaccggtttt aatttagcat ttctcagaat tccgcgaaca gcagcatcaa catcctgatt
      481 aaagagtttt tcagcctcat cttttgtaat tacaccatta gtattacgcc caatagcttt
      541 atctaattca gatttagcag ctgcaagaag tgatggactt tttgtaagca aatgaccgat
      601 gccaatagtg taatagcctt ctgtgtcttt atagatttta agtctaagac cttcatctat
      661 acgtaacatt tcaaatatat tcataatacc tcctaagcat cgatggatcc tgtttcctgt
      721 gtgaaattgt tatccgctca caattccaca cattatacga gccgatgatt aattgtcaac
      781 agggggatgg ggagtaagct gatcctgttt cctgtgtgaa aattgttatc cgctcacaat
      841 tccacacatt atacgagccg atgattaatt gtcaacaggg gggatgggga gtaagctcat
      901 cgatggatcg atccgggttt cccgnggnga aaatggtnat ccgcccncaa ttccccacat
      961 tatacgagcc ggaagccnta aagtgtaang cctggggngc ctaanggagg agctanctta
     1021 cattaattgc gttgcgctca ctgnccgctt tccnggtcgg gnaaacnggt cgngccagga
     1081 caccatcgaa ng
//
