LOCUS       sequence-240915-        4281 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone of Drosophila Moesin cDNA encoding the D 
            isoform..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4281)
  AUTHORS   Vilmos P, Kristo I, Szikora S, Jankovics F, Lukacsovich T, Kari B, 
            Erdelyi M
  TITLE     The actin-binding ERM protein Moesin directly regulates spindle 
            assembly and function during mitosis.
  JOURNAL   Cell Biol Int. 2016 Jun;40(6):696-707. doi: 10.1002/cbin.10607. Epub
            2016 Apr 6.
  PUBMED    27006187
REFERENCE   2  (bases 1 to 4281)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1002/cbin.10607"; journalName: "Cell Biol Int"; date: "2016-06";
            volume: "40"; issue: "6"; pages: "696-707"
COMMENT     Sequence Label: Moe-pDONR221
FEATURES             Location/Qualifiers
     source          1..4281
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     19..41
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     33..50
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     34..50
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    66..165
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1903..2002)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2019..2038)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2020..2038)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2043..2059)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2043..2059)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2161..2180)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2172..2981
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2239..2258)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3128..3716
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3617..3636
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3870..3887
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4046..4073)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4165..4251)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 cgctagcatg gatgttttcc cagtcacgac gttgtaaaac gacggccagt cttaagctcg
       61 ggccccaaat aatgatttta ttttgactga tagtgacctg ttcgttgcaa caaattgatg
      121 agcaatgctt ttttataatg ccaactttgt acaaaaaagc aggcttcacc atgtctccaa
      181 aagcgctaaa tgtgcgcgtc acgacaatgg acgcggaact ggagttcgcc attcagtcga
      241 cgacgacggg caagcaattg tttgaccagg tggtgaagac gatcggcctg cgagaggttt
      301 ggttctttgg actccagtac accgactcca agggcgactc cacatggatc aagctgtaca
      361 aaaaggtgat gaaccaggac gtgaagaagg agaatccctt gcagtttagg ttccgtgcca
      421 aattctatcc cgaggatgtg gccgaggagc tgatccagga cattacactg cgtctgttct
      481 acctgcaggt gaagaatgcc atactgaccg acgagatcta ttgtccgcca gagacatccg
      541 tgctgctcgc ctcgtacgcc gtccaggcgc gtcatggtga ccacaataag accacccaca
      601 cagccggctt tctggccaac gatcgcctgc tgccgcagcg cgtcatcgac cagcacaaga
      661 tgtccaagga cgagtgggag cagtcgatta tgacctggtg gcaggagcat cgcagcatgc
      721 tgcgcgagga tgccatgatg gagtatctga agatcgccca agacctggag atgtacggcg
      781 ttaactactt tgagatccgc aacaagaagg gcacggatct ttggctgggc gtagacgcac
      841 tgggtctgaa catttacgag caggacgata ggttgacgcc gaaaattggt ttcccatggt
      901 ccgagattcg caacatttcg ttctcggaga agaagttcat catcaagccg atcgacaaga
      961 aggctccgga ctttatgttc tttgcgccac gtgtccgcat caacaagcgc attctggccc
     1021 tctgcatggg caaccacgag ctgtacatgc gtcgccgcaa gccggacacc atcgatgtgc
     1081 agcagatgaa ggcgcaggcg cgcgaggaga agaatgccaa acagcaggaa cgtgagaagc
     1141 tgcagctggc gctggccgca cgcgaacgcg ctgaaaagaa gcagcaggag tacgaggatc
     1201 ggctaaagca gatgcaggag gatatggagc gttcgcagcg cgatctgctt gaggcgcagg
     1261 acatgatccg ccggctggag gagcagctga agcagctgca ggccgccaag gatgagctgg
     1321 agctgcgcca gaaggagctg caggcgatgc tgcagcgcct cgaggaggcc aagaatatgg
     1381 aggccgtcga gaagctcaag ctcgaggagg agatcatggc caagcagatg gaggtgcagc
     1441 gcattcagga cgaggtcaac gccaaggatg aggagacaaa gcgtctgcag gacgaagtgg
     1501 aagacgcccg acgcaagcag gctgaagccg ctgccgctct gctggccgcg tcgacaacgc
     1561 cgcagcatca ccacgtggcc gaggatgaga acgagaacga ggaggagctg acgaacggcg
     1621 atgccggtgg cgatgtgtcg cgcgacctgg acaccgacga gcatatcaag gaccccatcg
     1681 aggacagacg cacgctggcc gagcgcaacg aacgcttgca cgatcagctc aaggctctga
     1741 aacaagattt ggcgcagtct cgcgacgaga cgaaagagac ggcaaacgat aagattcatc
     1801 gcgagaacgt tcgccaggga cgtgacaagt acaagacgct ccgcgagatt cgtaagggca
     1861 acacaaagcg tcgcgtcgat cagtttgaga acatgtccag cgacccagct ttcttgtaca
     1921 aagttggcat tataagaaag cattgcttat caatttgttg caacgaacag gtcactatca
     1981 gtcaaaataa aatcattatt tgccatccag ctgatatccc ctatagtgag tcgtattaca
     2041 tggtcatagc tgtttcctgg cagctctggc ccgtgtctca aaatctctga tgttacattg
     2101 cacaagataa aaatatatca tcatgaacaa taaaactgtc tgcttacata aacagtaata
     2161 caaggggtgt tatgagccat attcaacggg aaacgtcgag gccgcgatta aattccaaca
     2221 tggatgctga tttatatggg tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga
     2281 caatctatcg cttgtatggg aagcccgatg cgccagagtt gtttctgaaa catggcaaag
     2341 gtagcgttgc caatgatgtt acagatgaga tggtcagact aaactggctg acggaattta
     2401 tgcctcttcc gaccatcaag cattttatcc gtactcctga tgatgcatgg ttactcacca
     2461 ctgcgatccc cggaaaaaca gcattccagg tattagaaga atatcctgat tcaggtgaaa
     2521 atattgttga tgcgctggca gtgttcctgc gccggttgca ttcgattcct gtttgtaatt
     2581 gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc gcaatcacga atgaataacg
     2641 gtttggttga tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct
     2701 ggaaagaaat gcataaactt ttgccattct caccggattc agtcgtcact catggtgatt
     2761 tctcacttga taaccttatt tttgacgagg ggaaattaat aggttgtatt gatgttggac
     2821 gagtcggaat cgcagaccga taccaggatc ttgccatcct atggaactgc ctcggtgagt
     2881 tttctccttc attacagaaa cggctttttc aaaaatatgg tattgataat cctgatatga
     2941 ataaattgca gtttcatttg atgctcgatg agtttttcta atcagaattg gttaattggt
     3001 tgtaacactg gcagagcatt acgctgactt gacgggacgg cgcaagctca tgaccaaaat
     3061 cccttaacgt gagttacgcg tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     3121 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     3181 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     3241 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     3301 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     3361 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     3421 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     3481 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     3541 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     3601 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     3661 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     3721 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     3781 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     3841 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     3901 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     3961 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatacg cgtaccgcta
     4021 gccaggaaga gtttgtagaa acgcaaaaag gccatccgtc aggatggcct tctgcttagt
     4081 ttgatgcctg gcagtttatg gcgggcgtcc tgcccgccac cctccgggcc gttgcttcac
     4141 aacgttcaaa tccgctcccg gcggatttgt cctactcagg agagcgttca ccgacaaaca
     4201 acagataaaa cgaaaggccc agtcttccga ctgagccttt cgttttattt gatgcctggc
     4261 agttccctac tctcgcgtta a
//
