LOCUS       sequence-24092-e        1080 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1080)
  TITLE     T4 Lysozyme Plasmids - Tray 2
REFERENCE   2  (bases 1 to 1080)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: T4 Lysozyme N40L K43A S44-[A] WT*
FEATURES             Location/Qualifiers
     source          1..1080
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    726..742
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(750..756,757..772,773..778))
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    821..837
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(845..851,852..867,868..873))
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
ORIGIN
        1 ctangaaaag cccgctcatt aggcgggctc agatctgctc atgttttgac agcttatcat
       61 cgataagctt cggcaataaa ttcttgatat gttttcatat gtttccttta aatattttaa
      121 ttagtaattg tctattcaag taattgtgaa tatactatca caattccaag agaaagtaaa
      181 cagctttata gatttttata cgcgtcccaa gtgccagttc taaacgttgt aatgactcgt
      241 tttgcgcgat taggtgtttg attataccat ctacttttag ctaagttaac tgctgcttca
      301 tcccagcgtt tttgttgaag catacgtaaa gagttagtaa atcctgccac accggtttct
      361 cccatttgga aaaccatatt aatcaatgca gcgcgacgaa ccgcatcaag agaatcataa
      421 accggtttta atttagcatt tctcagaatt ccgcgaacag cagcatcaac atcctgatta
      481 aagagttttt cagcctcatc ttttgtaatt acaccattag tattacgccc aatagcttta
      541 tctaattctg cagatgcagc agcaagaagt gatggacttt ttgtaagcaa atgaccgatg
      601 ccaatagtgt aatagccttc tgtgtcttta tagattttaa gtctaagacc ttcatctata
      661 cgtaacattt caaatatatt cataatacct cctaagcatc gatggatcct gtttcctgtg
      721 tgaaattgtt atccgctcac aattccacac attatacgag ccgatgatta attgtcaaca
      781 gggggatggg gagtaagctg atcctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
      841 cacacattat acgagccgat gattaattgt caacaggggg gatggggagt aagctcatcg
      901 atggatcgac cggggntttc ccnggggggn naaannggtn anccgcncnc aantnccncn
      961 cattatacga gccggaagca naaagnggta aagcccgggg gggcctaaag gagnggagct
     1021 ancttacntt aattggcgtt gcncncncng cccncnttnc cngncgggna aaccngncng
//
