LOCUS       sequence-240967-        7421 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Targeting expression to neuronal and glial cell types in mice, 
            non-human primates, and human retina.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7421)
  AUTHORS   Juttner J, Szabo A, Gross-Scherf B, Morikawa RK, Rompani SB, Hantz 
            P, Szikra T, Esposti F, Cowan CS, Bharioke A, Patino-Alvarez CP, 
            Keles O, Kusnyerik A, Azoulay T, Hartl D, Krebs AR, Schubeler D, 
            Hajdu RI, Lukats A, Nemeth J, Nagy ZZ, Wu KC, Wu RH, Xiang L, Fang 
            XL, Jin ZB, Goldblum D, Hasler PW, Scholl HPN, Krol J, Roska B
  TITLE     Targeting neuronal and glial cell types with synthetic promoter AAVs
            in mice, non-human primates and humans.
  JOURNAL   Nat Neurosci. 2019 Jul 8. pii: 10.1038/s41593-019-0431-2. doi: 
            10.1038/s41593-019-0431-2.
  PUBMED    31285614
REFERENCE   2  (bases 1 to 7421)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2019 Jul 8. pii: 10.1038/s41593-019-0431-2. doi: 
            10.1038/s41593-019-0431-2."
COMMENT     Sequence Label: 51_pAAV-ProC12-CatCh-GFP-WPRE
FEATURES             Location/Qualifiers
     source          1..7421
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     promoter        1662..1858
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1709..1844
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     regulatory      1865..1874
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1871..2797
                     /codon_start=1
                     /product="N-terminal fragment of channelrhodopsin-2 from 
                     the alga Chlamydomonas reinhardtii (Nagel et al., 2003)"
                     /label=ChR2
                     /note="blue light-activated cation channel"
                     /translation="MDYGGALSAVGRELLFVTNPVVVNGSVLVPEDQCYCAGWIESRGT
                     NGAQTASNVLQWLAAGFSILLLMFYAYQTWKSTCGWEEIYVCAIEMVKVILEFFFEFKN
                     PSMLYLATGHRVQWLRYAEWLLTCPVICIHLSNLTGLSNDYSRRTMGLLVSDIGTIVWG
                     ATSAMATGYVKVIFFCLGLCYGANTFFHAAKAYIEGYHTVPKGRCRQVVTGMAWLFFVS
                     WGMFPILFILGPEGFGVLSVYGSTVGHTIIDLMSKNCWGLLGHYLRVLIHEHILIHGDI
                     RKTTKLNIGGTEIEVETLVEDEAEAGAV"
     CDS             join(2801..2803,2804..2806,2807..3520)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2801..2803,2804..2806,2807..3517)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2846..2867)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3107..3126)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3454..3475
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    3548..4136
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3601..3621)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4019..4030)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    4168..4644
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(4367..4386)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   4684..4824
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   4684..4813
                     /label=AAV2 ITR
     rep_origin      4899..5354
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4986..5005)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5196..5217
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5371..5390)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5490..5512
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5550..5568)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5636..5740
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5741..5809,5810..6601)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5959..5978)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6772..7360
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7261..7280
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgttcaagc ctcctaccgc ctttgttatg caaacatatc
      181 aaacgccctc ctttgttatg caaaagggct ggaacggggc ctttgttatg caaatcgccc
      241 tccccgatcc ctttgttatg caaatttgac gaattcccac ctttgttatg caaacaaatc
      301 tcctaccctc ctttgttatg caaagtgaga ggggctgcac ctttgttatg caaaatgcgg
      361 cccctgagac ctttgttatg caaaaaccat gtacgcttgc ctttgttatg caaaccgcct
      421 gttgcttggc ctttgttatg caaagccacg cgattggcgc ctttgttatg caaagctcgg
      481 ttatgtacac ctttgttatg caaagctact ttaaacttgc ctttgttatg caaatcacga
      541 cctgaccgtc ctttgttatg caaaaacggt tgaaatagtc ctttgttatg caaaatgata
      601 ttgaatagtc ctttgttatg caaaaattta gatgccgacc ctttgttatg caaaggaatg
      661 ggcgtgctgc ctttgttatg caaattttcg ctgcgacagc ctttgttatg caaacatgct
      721 cgccactcac ctttgttatg caaaggtcta acaatgaccc ctttgttatg caaactacgt
      781 ggaatagatc ctttgttatg caaaccccga gtttttgaac ctttgttatg caaaatcagt
      841 aacttcattc ctttgttatg caaaatgtga cttaacctcc ctttgttatg caaatacacg
      901 ctagtcgcat ggacgtggat gctagctaaa acccagcagg ctcggttgtt gtagttctta
      961 cataatgact atcacctcgg caagggagtt ttaagcggca gcgggagaat tgccgcatga
     1021 acacaaactt tctctgtcga tggttcgcgg aactcttcgg cgattgtccg cccctttcaa
     1081 ccacctaaat agatgtgctt tttgggttga ggtgctgttc caaagaactg gcacgcatcg
     1141 aagcacgtca aaatcggctc gtagaaattc gcacgtattc cccgtgcctg gtgggtcgtc
     1201 gagattctgt gaaggagcac tgggaacgaa tgcgtgggtc accatacccg tagaatgctt
     1261 gtaacgccac tagcggcttg gtcatctttc cctaaaggct gcgtggtccc gaatgacgta
     1321 acgtcgatgg ccggttagcc caaaaatatg cccgtattta ctaaacctgt cgtgtctctc
     1381 ttcctgttgt ctctcgctag gtccatccct aagtcttatc gaaggtgcta ttttactctg
     1441 ctcttggaac acacccatct ccacgcacgt gataggtaca gcaagcgtta caggccatcg
     1501 tttcgtacga attttcgaaa agtaatcctc agaaatcgaa tattaccttt agttggaacg
     1561 cgcttccaaa gcgccgggcc ctgaagtgct atgggatggg cttctttcgc gtgtcctttt
     1621 ggccgatgtc tgactgacag attagctcga gatctgcgat ctgcatctca attagtcagc
     1681 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     1741 ttctccgccc catcgctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
     1801 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaagg
     1861 atccgccacc atggattatg gaggcgccct gagtgccgtt gggcgcgagc tgctatttgt
     1921 aacgaaccca gtagtcgtca atggctctgt acttgtgcct gaggaccagt gttactgcgc
     1981 gggctggatt gagtcgcgtg gcacaaacgg tgcccaaacg gcgtcgaacg tgctgcaatg
     2041 gcttgctgct ggcttctcca tcctactgct tatgttttac gcctaccaaa catggaagtc
     2101 aacctgcggc tgggaggaga tctatgtgtg cgctatcgag atggtcaagg tgattctcga
     2161 gttcttcttc gagtttaaga acccgtccat gctgtatcta gccacaggcc accgcgtcca
     2221 gtggttgcgt tacgccgagt ggcttctcac ctgcccggtc atttgcattc acctgtcaaa
     2281 cctgacgggc ttgtccaacg actacagcag gcgcaccatg ggtctgcttg tgtctgatat
     2341 tggcacaatt gtgtggggcg ccacttccgc catggccacc ggatacgtca aggtcatctt
     2401 cttctgcctg ggtctgtgtt atggtgctaa cacgttcttt cacgctgcca aggcctacat
     2461 cgagggttac cacaccgtgc cgaagggccg gtgtcgccag gtggtgactg gcatggcttg
     2521 gctcttcttc gtatcatggg gtatgttccc catcctgttc atcctcggcc ccgagggctt
     2581 cggcgtcctg agcgtgtacg gctccaccgt cggccacacc atcattgacc tgatgtcgaa
     2641 gaactgctgg ggtctgctcg gccactacct gcgcgtgctg atccacgagc atatcctcat
     2701 ccacggcgac attcgcaaga ccaccaaatt gaacattggt ggcactgaga ttgaggtcga
     2761 gacgctggtg gaggacgagg ccgaggctgg cgcggtaccc atggtgagca agggcgagga
     2821 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa
     2881 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt
     2941 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta
     3001 cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc
     3061 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta
     3121 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa
     3181 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa
     3241 cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa
     3301 gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac
     3361 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc
     3421 cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
     3481 cgccgggatc actctcggca tggacgagct gtacaagtga taagaattcg atatcaagct
     3541 tatcgataat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     3601 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
     3661 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
     3721 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
     3781 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
     3841 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
     3901 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg
     3961 gctgctcgcc tatgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
     4021 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
     4081 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcatcg
     4141 ataccgagcg ctgctcgaga gatctacggg tggcatccct gtgacccctc cccagtgcct
     4201 ctcctggccc tggaagttgc cactccagtg cccaccagcc ttgtcctaat aaaattaagt
     4261 tgcatcattt tgtctgacta ggtgtccttc tataatatta tggggtggag gggggtggta
     4321 tggagcaagg ggcaagttgg gaagacaacc tgtagggcct gcggggtcta ttgggaacca
     4381 agctggagtg cagtggcaca atcttggctc actgcaatct ccgcctcctg ggttcaagcg
     4441 attctcctgc ctcagcctcc cgagttgttg ggattccagg catgcatgac caggctcagc
     4501 taatttttgt ttttttggta gagacggggt ttcaccatat tggccaggct ggtctccaac
     4561 tcctaatctc aggtgatcta cccaccttgg cctcccaaat tgctgggatt acaggcgtga
     4621 accactgctc ccttccctgt ccttctgatt ttgtaggtaa ccacgtgcgg accgagcggc
     4681 cgcaggaacc cctagtgatg gagttggcca ctccctctct gcgcgctcgc tcgctcactg
     4741 aggccgggcg accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg
     4801 agcgagcgcg cagctgcctg caggggcgcc tgatgcggta ttttctcctt acgcatctgt
     4861 gcggtatttc acaccgcata cgtcaaagca accatagtac gcgccctgta gcggcgcatt
     4921 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     4981 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     5041 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     5101 caaaaaactt gatttgggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     5161 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     5221 aacactcaac cctatctcgg gctattcttt tgatttataa gggattttgc cgatttcggc
     5281 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt
     5341 aacgtttaca attttatggt gcactctcag tacaatctgc tctgatgccg catagttaag
     5401 ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc
     5461 atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc
     5521 gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa
     5581 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
     5641 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     5701 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     5761 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     5821 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     5881 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     5941 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     6001 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     6061 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     6121 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     6181 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     6241 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     6301 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     6361 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     6421 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     6481 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     6541 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     6601 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     6661 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     6721 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     6781 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     6841 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     6901 gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     6961 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     7021 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     7081 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     7141 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     7201 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     7261 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     7321 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     7381 tttacggttc ctggcctttt gctggccttt tgctcacatg t
//
