LOCUS       sequence-240983-        4705 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GBPart - RedF Luciferase CDS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4705)
  AUTHORS   Sarrion-Perdigones A, Chang L, Gonzalez Y, Gallego-Flores T, Young 
            DW, Venken KJT
  TITLE     Examining multiple cellular pathways at once using multiplex 
            hextuple luciferase assaying.
  JOURNAL   Nat Commun. 2019 Dec 13;10(1):5710. doi: 10.1038/s41467-019-13651-y.
  PUBMED    31836712
REFERENCE   2  (bases 1 to 4705)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-12-13"; volume: "10(1):5710. doi"; pages: " 
            10.1038/s41467-019-13651-y"
COMMENT     Sequence Label: pRedF
FEATURES             Location/Qualifiers
     source          1..4705
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(1417..1435)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1418..1435)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(1453..1469)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1453..1469)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1466..1488)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1477..1493
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1501..1507,1508..1525,1526..1531))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1546..1567
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1684..1701)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1855..2443)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1935..1954)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2614..3405,3406..3474))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3237..3256
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3475..3579)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3657..4112)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3794..3815)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4006..4025
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4238..4260
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4252..4269
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4253..4269
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4276..4295
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4276..4294
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      4325..4334
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      4329..4338
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accctgcgcg agctggtcca cagcctgggt atcagcaagc cgaccatcgt tttctcgagc
       61 aagaagggcc tcgacaaggt tatcaccgtg caaaaaaccg tcaccaccat caagaccatc
      121 gttatcctgg attccaaggt tgactaccgc ggttaccagt gcctcgacac cttcatcaag
      181 cgtaacaccc cacccggctt ccaagcctcc tccttcaaaa ccgtggaggt tgatcgcaag
      241 gagcaggtgg ccctgatcat gaactcctcc ggctccaccg gtctgccaaa gggcgtgcag
      301 ctgacccacg agaacaccgt cacccgcttc tcgcatgccc gtgatcccat ctacggcaac
      361 caggtgtccc caggcaccgc cgtcctgacc gtcgtgcccc tccaccacgg cttcggcatg
      421 ttcaccactc tcggctactt gatctgcggc ttccgtgtgg tgatgctgac taagttcgac
      481 gaggagactt tcctcaagac cctgcaagac tacaagtgca cttacgtgat cctggtgccg
      541 accctgttcg ccatcctgaa caagagcgag ctgttgaaca aatacgacct gtcgaacctg
      601 gtggagatcg cttccggcgg cgccccactg tcgaaggaag ttggcgaggc cgttgctcgc
      661 cgcttcaacc tgcccggcgt ccgtcagggt tacggcctga ccgagactac ctcggctatc
      721 atcatcaccc ccgagggcga cgataagccc ggcgcttccg gcaaggttgt tccactcttc
      781 aaagccaagg tgatcgacct ggacaccaag aagtccctgg gtcccaaccg tcgcggagaa
      841 gtgtgcgtca agggccccat gctgatgaag ggctacgtca acaacccaga ggccaccaag
      901 gagttgatcg acgaggaggg ctggctgcac actggtgaca tcggttacta cgacgaagag
      961 aagcacttct tcatcgttga ccgcctgaag tccttgatca agtacaaggg ctaccaggtt
     1021 ccccccgccg agctcgagag cgtcctgctg cagcacccct ccattttcga tgccggagtg
     1081 gccggtgtcc ccgatcccgt ggctggcgag ctgccaggtg ccgtcgtcgt gctggagtcc
     1141 ggcaagaaca tgaccgagaa ggaagtgatg gattacgtgg cctcgcaagt gtccaacgcc
     1201 aagcgcctgc gcggcggcgt gcgcttcgtg gatgaggtgc ccaagggcct caccggaaag
     1261 atcgacggtc gcgctatccg cgagatcctc aagaagcccg tggccaagat gtgagcttcg
     1321 agaccactca tcgcccaatc actagtgaat tcgcggccgc ctgcaggtcg accatatggg
     1381 agagctccca acgcgttgga tgcatagctt gagtattcta tagtgtcacc taaatagctt
     1441 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     1501 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     1561 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     1621 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     1681 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     1741 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     1801 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     1861 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     1921 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     1981 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     2041 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     2101 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     2161 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     2221 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     2281 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     2341 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     2401 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     2461 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     2521 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     2581 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     2641 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     2701 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgcgaccc
     2761 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     2821 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     2881 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     2941 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     3001 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     3061 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     3121 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     3181 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     3241 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     3301 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     3361 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     3421 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     3481 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     3541 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     3601 acctgatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggaaat
     3661 tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt
     3721 taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg
     3781 gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt
     3841 caaagggcga aaaaccgtct atcagggcgc tggcccacta cgtgaaccat caccctaatc
     3901 aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg
     3961 atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa
     4021 aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc
     4081 cgccgcgctt aatgcgccgc tacagggcgc gtccattcgc cattcaggct gcgcaactgt
     4141 tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
     4201 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
     4261 acggccagtg aattgtaata cgactcacta tagggcgaat tgggcccgac gtcgcatgct
     4321 cccggccgcc atggcggccg cgggaattcg attgggcgat gagtggtctc gaatggagaa
     4381 catggagaac gacgagaaca ttgtggtggg acccaagccg ttctacccga tcgaggaggg
     4441 ttccgctggc acccagctgc gcaagtacat ggagcgctac gctaagctgg gtgctatcgc
     4501 cttcaccaac gccgtgaccg gtgtggatta ctcctacgcc gagtacctgg agaagtcctg
     4561 ctgcctcgga aaggccctgc agaactacgg tctggtcgtg gatggccgca ttgccctctg
     4621 ctcggagaac tgcgaggagt tcttcattcc cgtgatcgct ggtctgttca tcggagtcgg
     4681 cgttgcccca accaacgaga tctac
//
