LOCUS       sequence-240984-       20781 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive expression of human fat4 protein fused to citrine 
            fluorescent protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 20781)
  AUTHORS   Loza O, Heemskerk I, Gordon-Bar N, Amir-Zilberstein L, Jung Y, 
            Sprinzak D
  TITLE     A synthetic planar cell polarity system reveals localized feedback 
            on Fat4-Ds1 complexes.
  JOURNAL   Elife. 2017 Aug 18;6. pii: 24820. doi: 10.7554/eLife.24820.
  PUBMED    28826487
REFERENCE   2  (bases 1 to 20781)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2017-08-18"; volume: "6. pii: 24820. doi"; pages: " 
            10.7554/eLife.24820"
COMMENT     Sequence Label: pEv-Fat4-citrine
FEATURES             Location/Qualifiers
     source          1..20781
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     629..648
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      774..783
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(15744..15746,15747..15749,15750..16460)
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(15789..15810)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(16050..16069)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     16397..16418
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             16470..16499
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             16515..16532
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(16555..16572)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    16561..16785
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      16831..17259
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(16918..16937)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     17128..17149
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(17268..17288)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        17273..17602
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      17453..17588
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     17515..17534
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        17650..17697
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             17716..18090
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    18220..18341
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(18257..18276)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     18311..18330
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(18390..18406)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(18390..18406)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(18403..18425)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    18414..18430
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(18438..18444,18445..18462,18463..18468))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     primer_bind     complement(18621..18638)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(18792..19380)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(18872..18891)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(19551..20342,20343..20411))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     20174..20193
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(20412..20516)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(20591..20610)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctg
      661 gctagccacc gcggcccagc cggccaggcg cgccgtacga agcttggtac cgagctcgga
      721 tccactccag tgtggtggaa ttctgcagat atccagcaca gtggcggccg ctcgccacca
      781 tggacttagc accagacagg gctactggcc gcccgtggct cccgttgcac actctatcag
      841 tatctcagct ccttcgagtg ttttggctac tgtcattgct tccggggcag gcctgggtcc
      901 acggggccga gccgcgccag gtgttccaag tgctggaaga gcaacctcca ggcactctgg
      961 taggcaccat ccagacgcgc cccggcttca cctacaggct cagcgaaagc cacgccctgt
     1021 ttgccataaa cagtagcacc ggagccctgt acaccacctc caccatcgac cgcgagagcc
     1081 tgcccagcga cgtgatcaac ctggtggtcc tttccagcgc gcccacctac cccaccgaag
     1141 tgcgagtgct ggtgcgggac ctcaatgaca acgcccccgt ttttccggac ccctctatcg
     1201 tggtcacttt caaggaagac agtagcagcg gacgccaagt catcttagac accgccaccg
     1261 actcggacat cggctcaaac ggtgtggacc accgctccta ccgcatcatc cgcggcaatg
     1321 aggcggggcg cttccgtctg gacatcaccc tgaacccgag cggcgaggga gcgttcctgc
     1381 atctggtgtc caagggcgga ctggaccgtg aggtcactcc gcagtaccag ctcctggttg
     1441 aggtggagga caagggtgag cctaagcggc ggggctacct tcaggtaaac gtgactgtgc
     1501 aagacattaa tgacaacccc ccggtttttg gcagttctca ctaccaggcg ggggtgcctg
     1561 aggacgcggt tgtgggttcc agcgtcctcc aggtggcggc ggcggacgcg gacgagggca
     1621 ccaacgcgga catccgctat cgcctgcagg acgaggggac ccccttccaa atggaccctg
     1681 agacgggact tatcacggtg cgggagcccc tggacttcga agctcggcgc caatactcgc
     1741 ttacggtgca ggcgatggac agaggcgtgc cttccctcac tgggcgcgcc gaggcgctga
     1801 ttcagctgct ggacgtgaat gacaatgacc cggtagtgaa gttccgctac ttcccggcca
     1861 cctcgcgcta cgcctcggta gatgagaatg ctcaagtggg cactgtggtg gctctgctca
     1921 ccgtgacgga cgcagattct cccgcggcca acgggaacat ctccgtgcaa attctcgggg
     1981 gcaatgagca gcgccacttt gaagtgcaaa gcagcaaagt gccgaacctg agcctaatca
     2041 aggtggccag cgccttggac cgcgagcgca tcccttccta caacctcaca gtttccgtct
     2101 ctgataacta cggggcgccc cctggcgcag cagtcctggc gcgctcttct gtggcaagcc
     2161 tggtgatttt tgttaatgac atcaatgacc atcctcctgt cttttcacag caagtgtaca
     2221 gagtgaacct gagcgaggag gcgcctccgg gaagctatgt gagtgggata tctgccactg
     2281 atggcgactc tggtctcaat gctaatctgc gttacagcat tgtctctggc aatggactgg
     2341 gatggttcca tatcagtgaa catagcggcc tcgtgaccac tgggtcctct gggggcctgg
     2401 accgtgaact tgcttcccag attgttctga atataagtgc ccgggaccag ggagttcacc
     2461 ccaaggtgtc ctatgcccag cttgtagtaa ctctcctaga tgtgaatgat gaaaagccag
     2521 tatttagcca gccagaaggg tatgatgtgt ctgtggttga gaatgcccca acagggacag
     2581 aactgttgat gctcagggca actgacgggg acctgggtga caacggaaca gtgcgcttct
     2641 ccttacaaga ggcagagact gaccggaggt ccttccgtct ggatcctgtg tctgggaggt
     2701 tgagtactat ttcctccttg gacagagaag agcaagcctt ctactccctg ttggttctgg
     2761 ccacagatct gggctcccct ccccagtcat caatggctcg cataaatgtg agtcttctgg
     2821 atataaatga taacagccct gtcttctacc cggtccaata ctttgctcac attaaggaga
     2881 atgagcctgg aggtagctac atcaccactg tgtctgccac tgacccagac ttgggtacca
     2941 atggtactgt caaatatagc atatctgctg gggacaggtc tcggtttcag gtcaatgctc
     3001 agagtggggt tatttctaca agaatggccc tagacagaga agaaaaaaca gcttatcagt
     3061 tgcaaatagt agctactgat ggtggcaatt tacaatctcc caaccaggca atagtaacca
     3121 tcactgtatt ggacactcaa gacaacccac ctgtattcag tcaggttgcc tacagctttg
     3181 tggtttttga gaacgtggtg ctgggatatc atgtgggtag tgtgtctgca tccaccatgg
     3241 atctcaattc caacatcagt tatctcatta ctactgggga tcagaaaggt atgtttgcta
     3301 tcaaccaggt cactgggcag cttaccacag caaatgtgat tgatagagaa gagcaatcct
     3361 tttatcagct gaaggtagtg gccagtgggg gcacagtgac tggagacact atggttaaca
     3421 taacagttaa ggatttgaat gacaactctc cccatttcct tcaggcaata gagagtgtaa
     3481 atgtggtgga gaattggcag gcaggtcaca gcattttcca ggccaaagct gtggaccctg
     3541 atgaaggtgt caatggcatg gtactctata gtctgaagca aaaccccaag aacctgtttg
     3601 ctatcaatga aaagaatggc actattagtc tgcttgggcc cctggatgtt catgctggct
     3661 cctaccaaat agagatcttg gcatctgaca tgggtgtccc acagctctcc tctagtgtca
     3721 tcctaacagt ttatgtccat gatgtaaatg acaattcacc agtgtttgac caactctctt
     3781 atgaagtcac cctttctgag tcagaacctg tgaattctcg attctttaaa gtacaagctt
     3841 ctgataagga ttcaggagca aatggtgaaa ttgcatacac cattgctgaa ggaaatacag
     3901 gggatgcttt tggcatattc ccagatggtc aattgtatat aaaaagtgaa ctggaccgtg
     3961 aacttcaaga cagatatgtt ttaatggttg ttgcttctga cagagcagtg gaacccctta
     4021 gtgctactgt gaatgttact gtaattttag aagatgtaaa tgataacaga cctcttttta
     4081 acagtaccaa ttacacattt tacttcgaag aagagcagag ggctgggtcg tttgtgggca
     4141 aagtaagtgc tgtagataaa gactttgggc caaatggaga agtaaggtat tcttttgaaa
     4201 tggtgcagcc agattttgag ttgcatgcca tcagtgggga aattacaaat actcatcagt
     4261 ttgacaggga gtctcttatg aggcggagag ggactgctgt gtttagcttt acagtcatag
     4321 caacagatca ggggatccct cagcctctca aggatcaggc cactgtacat gtttacatga
     4381 aggatataaa tgataatgct cccaaatttt taaaagactt ttaccaagct acaatatcag
     4441 aatcagcagc caatctgaca caagtgttaa gagtatctgc ctcagatgtt gatgaaggta
     4501 ataatggact tattcactat tctataataa aaggaaatga agaaagacag tttgctatag
     4561 acagtacctc tggtcaggta acactaattg gcaaattaga ctatgaagca acacctgcct
     4621 attcccttgt aattcaagca gtggattcag ggacaatccc cctcaattca acgtgtactt
     4681 taaatattga tattttagat gaaaatgaca ataccccttc tttccctaaa tcaacactct
     4741 ttgttgatgt tttggaaaac atgagaattg gtgaactcgt gtcctctgtt actgcaactg
     4801 attccgattc aggtgacaat gctgatttat attacagtat tactgggact aacaaccacg
     4861 gaacttttag cattagccca aacactggga gtatttttct tgccaaaaaa ctggactttg
     4921 aaacacagtc tttgtataaa ttaaatataa cagcaaaaga ccaaggaaga cctcctcgtt
     4981 catctacaat gtcagtggtt attcacgtga gggactttaa tgacaatcct cctagctttc
     5041 ctcctggaga tattttcaag tctattgttg agaacattcc cattggtaca tctgtcattt
     5101 cagtgactgc acatgaccct gatgcagaca ttaatggtca actatcctac acaatcattc
     5161 aacagatgcc aagaggcaac cactttacca tagatgaagt caaagggact atatatacta
     5221 atgctgaaat agatcgggaa tttgctaatc tctttgagtt gactgtaaaa gccaatgatc
     5281 aagctgtgcc aatagaaact agacggtatg ctttgaagaa cgtgaccatt ttggttacag
     5341 acctcaatga caatgtccca atgtttatat cacaaaacgc ccttgctgca gacccatcag
     5401 ctgtgattgg ttccgttctg acaacaatta tggctgctga cccagatgaa ggtgctaatg
     5461 gagaaataga gtatgagatc atcaatgggg acacagacac cttcattgtt gatcgttata
     5521 gtggagacct gagagtggct tcagcgttgg tgccttcaca gttgatctac aatctcatag
     5581 tttcagcaac agaccttggg cctgaaagga ggaaatcgac cactgaattg accatcattc
     5641 ttcagggcct tgatggacct gtttttactc aacccaaata tataactatt ttgaaggaag
     5701 gagaacccat tggcacaaac gtgatatcaa tagaagcagc tagccccaga ggatctgagg
     5761 ccccagtgga gtattatatt gtttcagttc gttgtgaaga aaaaactgtt ggacgcctct
     5821 ttactattgg acgacatact ggtataattc agaccgcagc cattctggac cgggagcaag
     5881 gagcatgtct ttacctggtg gatgtttatg ccatagaaaa atcaactgct tttcccagaa
     5941 cacagagagc agaggtagaa ataacacttc aggatatcaa tgacaatcca ccagtatttc
     6001 caacggacat gctggatctc acggtagagg agaacattgg agatggctct aagattatgc
     6061 agctgacagc catggatgct gatgagggtg caaatgctct cgtcacatac actatcatta
     6121 gtggagctga tgatagtttt cgcatcgacc cagaatccgg agatctgata gcaaccaggc
     6181 ggttggacag ggaacgccgc tccaaatatt cactgctagt tcgtgctgat gatggtcttc
     6241 agtcctcgga tatgagaatt aatatcactg tcagtgatgt gaatgaccat acacccaaat
     6301 tttccagacc cgtgtactct tttgacattc ctgaggacac aatccctggt tctttggtag
     6361 cagccatttt agccacggat gatgactctg gtgtgaatgg agaaattaca tatattgtga
     6421 atgaagatga tgaagatggc atctttttcc tgaatcctat tactggggtc tttaatttga
     6481 ctcgattatt agattatgaa gtacagcaat attatatcct cactgttcga gcagaagacg
     6541 gtgggggaca atttactacc atcagagttt atttcaatat tctagatgta aatgataatc
     6601 cacctatttt cagcttgaat tcatacagca catctttaat ggagaatcta cctgtgggat
     6661 ctactgttct tgtgtttaat gttactgatg cagatgatgg catcaactct caattgactt
     6721 atagcattgc ttcaggtgat agccttgggc agtttactgt tgacaagaat ggtgtactca
     6781 aagtcctaaa agctttggat cgggaaagtc agtccttcta caacttggtt gttcaagtgc
     6841 atgacctgcc acagattcca gcctccagat tcacaagcac tgctcaagtc tccattattt
     6901 tgttggatgt aaatgataac ccaccgacat ttctttcccc taaattgaca tacattccag
     6961 aaaatacacc tattgatact gttgttttca aagctcaagc aactgaccca gatagtggcc
     7021 caaacagcta tattgagtac actctgctga accctttggg aaacaagttc agtattggga
     7081 ccattgatgg tgaagtgagg ctcactggag aactggacag agaagaagtt tctaattata
     7141 ctctaacagt ggtggctaca gacaaaggtc aaccatctct ctcttcatct acagaggttg
     7201 tagttatggt acttgacatc aatgataaca accccatctt tgcacaagct ttgtataaag
     7261 tggagattaa tgaaaacaca cttactggaa cagatataat acaagtgttc gcagcagatg
     7321 gagatgaagg cacaaatgga caggttcgct atggcattgt taatggtaat accaatcagg
     7381 aatttcggat agactctgtc acaggtgcca tcactgtcgc taaacctttg gatagagaaa
     7441 agacccctac ctaccattta actgttcagg caacagatcg aggcagcaca cccagaactg
     7501 atacctccac ggtcagcatt gttctactgg atattaatga ctttgttcct gtatttgagc
     7561 tatctccata ttctgtaaat gtccctgaga atttagggac actacccaga acaattcttc
     7621 aggtggtggc aagagatgat gatcgaggat ctaacagcaa actctcatat gttctgtttg
     7681 gtggtaatga agacaatgct tttactctct cagccagtgg agaacttgga gtaacacaga
     7741 gtctggatcg ggaaacaaaa gagcgctttg tcttaatgat tacagctaca gattcaggat
     7801 cccctgcctt gactggaact ggaacaatca acgtcatagt agatgatgtc aatgacaatg
     7861 tccccacatt tgccagtaaa gcgtatttca caacaattcc tgaggatgca ccaactggaa
     7921 cagatgtttt attggtaaat gcctcagatg ctgatgcttc aaagaatgca gttataagtt
     7981 ataggatcat cggtggaaac tctcagttca cgatcaaccc atcgacagga caaatcatca
     8041 ccagcgcatt gttagatagg gaaacaaaag ataattatac tttggtagtg gtctgcagtg
     8101 atgcgggatc cccagagcct ctttccagtt ccaccagtgt gcttgtcact gtgactgatg
     8161 tcaatgacaa tccaccaaga tttcagcatc acccatatgt cactcacatc ccatctccta
     8221 ctcttccagg ttcctttgtc tttgcggtta cagtcacaga tgctgatatt ggaccaaatt
     8281 ctgaactgca ttattctctt tcgggtagaa attctgaaaa atttcacatt gacccactga
     8341 ggggagccat tatggccgcc ggaccactaa acggagcttc agaagtgaca ttttctgtgc
     8401 atgtaaaaga tggtggctca tttccaaaga cagattctac aacagtgact gttagattcg
     8461 tgaataaggc cgatttccct aaagtcagag ccaaagaaca aacgttcatg tttcctgaaa
     8521 accaaccagt cagctctctt gtcaccacca tcacaggatc ctctttaaga ggagaaccta
     8581 tgtcatatta tatcgcaagt gggaatcttg gcaatacttt ccagattgat cagttaacag
     8641 ggcaggtgtc tattagtcaa cctctggatt ttgaaaagat acaaaaatat gttgtatgga
     8701 tagaggccag agacggtggt ttccctcctt tctcctctta cgagaaactt gatataacag
     8761 tattagatgt caatgataat gccccaattt ttaaggaaga cccatttata tctgaaatat
     8821 tggaaaacct ttcccctcga aaaatactta ctgtttcggc aatggacaag gacagtggac
     8881 ccaatggaca gttagattat gaaattgtta atggcaacat ggaaaatagt ttcagtatca
     8941 atcatgctac tggtgaaatt agaagcgtta gacctttgga cagggaaaaa gtatctcatt
     9001 atgtcctaac cataaaatca tcagacaaag ggtccccgtc tcagagtact tcagtaaaag
     9061 tcatgattaa cattttagat gaaaatgata atgcccctag gttttctcag atatttagtg
     9121 cccatgttcc tgaaaattcc cccttaggat acacagttac ccgtgtcaca acttctgatg
     9181 aagacattgg gatcaatgca attagtagat attctataat ggatgcaagt cttccattta
     9241 caattaatcc cagcacaggg gatattgtca taagcagacc tttaaatagg gaagatacag
     9301 accgttacag aattcgagtt tccgcacatg attctgggtg gactgtaagt acagatgtca
     9361 caatatttgt gacagacatc aatgacaatg ctccaagatt tagcagaact tcctattatt
     9421 tagattgccc tgaacttact gagattggct ccaaagtaac tcaggtattt gcaacagatc
     9481 ctgatgaggg atcaaatgga caagtgtttt atttcataaa atcccaatca gaatatttca
     9541 ggattaatgc caccactgga gagattttca ataaacagat cttaaaatac caaaatgtca
     9601 ctggcttcag taatgtgaat atcaacaggc atagttttat agtgacatct tcagatcgag
     9661 gtaaaccttc cttaattagt gagacaacag ttaccatcaa tatagtggac agtaatgaca
     9721 atgcacctca atttcttaaa agtaaatatt tcactccagt caccaaaaat gttaaggttg
     9781 gtacgaagtt aatcagagtt acagcaatag atgacaaaga ttttggactg aattcagaag
     9841 tggagtattt catttctaat gataaccatt taggaaaatt taagttggac aatgatacgg
     9901 ggtggatttc agtagcatcc tccctgattt ctgacttgaa ccaaaacttt tttatcacag
     9961 tcactgcaaa ggataaggga aaccctccac tttcttccca agcaactgtt cacataactg
    10021 tcactgagga aaactaccat acacctgaat tctctcaaag ccacatgagt gcaaccatcc
    10081 ctgagagcca tagcattggg tccattgtca gaactgtttc tgcaagagat agagatgcag
    10141 cgatgaatgg cttgattaag tacagcattt cttcaggaaa tgaagaaggc atttttgcaa
    10201 tcaattcttc tacaggtata ttaacactag ccaaagctct tgattatgag ctatgccaga
    10261 aacacgaaat gacgattagt gctatagatg gaggatgggt tgcaagaact ggttactgca
    10321 gtgtgaccgt aaatgtgatt gatgtgaatg ataattctcc agtattcctc tctgatgact
    10381 atttccctac tgttttggaa aatgccccaa gtggaacaac agttatccac ctaaatgcaa
    10441 cagatgctga ctctggaaca aatgctgtga ttgcgtatac tgtacagtca tctgacagtg
    10501 acctctttgt cattgaccct aacacaggag tcataaccac tcaaggcttc ttggattttg
    10561 aaaccaagca gagctaccat cttactgtga aagccttcaa tgtccccgat gaggaaaggt
    10621 gtagctttgc cactgttaat atacaattaa aagggacaaa tgaatatgtg ccccgttttg
    10681 tttccaaact ttactatttt gaaatctcag aagcagctcc taaaggtact attgttggag
    10741 aagtgtttgc tagcgaccgt gatttgggca ctgatgggga ggtacactat ttgatttttg
    10801 gtaatagtcg aaagaagggt ttccagatca ataagaagac tggacagatt tatgtttctg
    10861 gaattcttga tcgagaaaaa gaagaaaggg tgtctttgaa ggtattggcc aagaactttg
    10921 gcagcattag aggtgcagat atagatgagg tcactgtaaa tgtcaccgtg cttgatgcaa
    10981 atgacccacc catttttact ctaaacatct acagtgtgca gatcagtgaa ggggtcccaa
    11041 taggaactca tgtgaccttt gtcagtgcct ttgactcaga ctccatcccc agctggagca
    11101 ggttttctta cttcatcgga tcagggaatg aaaatggtgc cttttctatt aatccgcaga
    11161 caggacagat caccgttact gcagaattag atcgagaaac ccttcccatc tataatctct
    11221 cagttttggc tgttgattca gggaccccct cagctacagg tagtgcctct ttattagtca
    11281 ccctggaaga tataaatgat aacgggccca tgctgactgt cagtgaagga gaagtcatgg
    11341 aaaacaaacg gccagacact ttggtgatga cccttcagtc cactgaccct gatctccctc
    11401 caaatcaagg tccctttact tattacttgc tgagcacagg tcctgccacc agttatttca
    11461 gtctgagcac tgctggagtt ctgagcacaa ccagagagat tgacagagag cagattgcag
    11521 acttctatct gtctgtggtt accaaggatt ctggtgttcc tcaaatgtct tccacaggaa
    11581 ctgtgcatat cacagttata gaccaaaatg acaatccttc acagtctcgg acggtggaga
    11641 tatttgttaa ttattatggt aacttgtttc ccggtgggat tttaggctct gtgaagccac
    11701 aggatccaga tgtgttagac agcttccact gctcccttac ttcaggagtt accagcctct
    11761 tcagtattcc agggggtact tgtgatctga attcccagcc aaggtccaca gatggcacgt
    11821 ttgatctgac tgtccttagc aatgatggag ttcacagcac agtcacgagc aacatccgag
    11881 ttttctttgc tggattttcc aatgccacag tggataacag catcttactt cgtctcggcg
    11941 taccaacagt aaaggacttc ttgaccaacc actatcttca ttttttacgc attgccagct
    12001 cacagctgac aggcttaggg actgctgtgc aactgtacag tgcatatgaa gagaacaata
    12061 gaacgtttct tttggcagct gtgaagcgaa atcataatca gtatgtgaat cccagtggcg
    12121 tagccacctt ctttgaaagc atcaaagaga tccttctccg gcagagtgga gtaaaggtgg
    12181 aatctgtgga tcatgactcc tgtgtgcatg gcccatgtca gaatggaggg agctgtctac
    12241 gaagattggc tgtgagctcc gtattaaaaa gccgtgagag tcttccagtc atcatcgtgg
    12301 caaatgaacc tctgcagcct ttcttatgca agtgtctgcc aggatatgcg ggtagctggt
    12361 gtgaaataga tatagatgaa tgtcttccat caccttgcca caatggtgga acctgtcaca
    12421 atttagtggg aggattttca tgcagctgcc cagatggctt cactggtagg gcgtgtgaga
    12481 gagatatcaa tgagtgcctg cagagtcctt gcaagaatgg tgccatctgc cagaattttc
    12541 caggaagctt caactgtgtt tgcaaaactg gatacacagg gaaaatgtgt gaatcttcag
    12601 tcaattactg tgaatgcaac ccctgcttta atggtggttc ctgccaaagt ggtgtggatt
    12661 cttattattg tcattgtcca tttggtgtct ttggaaaaca ctgcgagttg aacagttatg
    12721 gatttgagga gttatcatac atggaatttc caagcttgga ccccaataac aactatattt
    12781 atgtcaaatt tgccacgatt aaaagtcatg ccttattgct ttacaactat gacaaccaga
    12841 caggcgaccg ggctgagttt ttggcccttg aaattgccga agaaagacta agattctctt
    12901 ataatttagg cagtggtaca tataagctca ccaccatgaa gaaggtgtca gatggacatt
    12961 ttcacactgt gattgccagg agagcaggaa tggcagcctc cttaactgtg gactcctgtt
    13021 ctgagaacca agagccagga tattgtactg tcagtaatgt ggcagtttca gatgactgga
    13081 ctcttgatgt tcagccaaat agagttacag ttggaggtat cagatctcta gaaccaatcc
    13141 ttcagagaag aggacacgtg gaaagccatg attttgttgg gtgtataatg gagtttgcag
    13201 tcaatggaag gcctctggaa cccagccaag ctttggcagc acaaggcatc ctagatcaat
    13261 gccctaggct ggaaggcgct tgtactcgca gcccatgcca acatggtggc acatgtatgg
    13321 attactggtc atggcagcag tgtcattgca aagagggact cactgggaaa tactgtgaaa
    13381 aatctgttac tcctgacact gccttatcat tagaaggcaa agggcgcttg gactaccaca
    13441 tgagtcagaa tgagaagcgg gaatatttgt taaggcaaag cttacgaggt gccatgttgg
    13501 agccttttgg tgtgaacagt ctggaagtaa aatttaggac cagaagcgag aatggcgttt
    13561 taatccatat ccaagaaagc agcaattaca ctactgtgaa gattaagaat ggcaaagtat
    13621 attttacatc cgatgcagga attgctggga aagtggagag aaatattcct gaagtatatg
    13681 ttgcagacgg ccactggcac acttttctaa ttgggaaaaa tggaacagca acagtattgt
    13741 ctgttgacag aatatataac agagatatta tccaccctac tcaggacttc ggtggccttg
    13801 atgtgcttac tatatcactt ggaggaattc cacccaatca agcacatcga gatgcccaaa
    13861 cagcaggttt tgatggctgc attgcttcta tgtggtatgg tggagaaagt cttcctttca
    13921 gcgggaagca tagcttggcc tccatctcaa aaacagatcc ctcagtgaag attggctgcc
    13981 gtggcccgaa catttgtgcc agcaacccct gctggggtga tttgctgtgc attaatcagt
    14041 ggtatgccta caggtgtgtc cctcctgggg actgtgcctc ccacccgtgc cagaatggtg
    14101 gcagctgtga gccaggcctg cactccggct tcacctgtag ctgcccagac tcgcacacgg
    14161 gaaggacctg tgagatggtg gtggcctgtc ttggcgtcct ctgtcctcag gggaaggtgt
    14221 gcaaagctgg aagtcctgcg gggcatgtct gtgttctgag tcagggccct gaagagatct
    14281 ctctgccttt gtgggctgtg cctgccatcg tgggcagctg cgcaaccgtc ttggccctcc
    14341 tggtccttag cctgatcctg tgtaaccagt gcagggggaa gaaggccaaa aatcccaaag
    14401 aggagaagaa accgaaggag aagaagaaaa agggaagtga gaacgttgct tttgatgacc
    14461 ctgacaatat ccctccctat ggggatgaca tgactgtgag gaagcagcct gaagggaacc
    14521 caaaaccaga tatcattgaa agggaaaacc cctaccttat ctatgatgaa actgatattc
    14581 ctcacaactc agaaaccatc cccagcgccc ctttggcatc tccagagcag gagatagagc
    14641 actatgacat tgacaacgcc agcagcatcg ccccttcgga tgcagacatc attcaacact
    14701 acaagcagtt ccgcagccac acaccaaaat tttcaatcca gaggcacagt cccctaggct
    14761 ttgcaaggca atcccccatg cccttaggag caagcagttt gacttaccag ccttcatatg
    14821 gtcaaggttt gagaaccagc tccctaagcc actcagcatg cccaactccc aaccctctgt
    14881 ctcgacacag tccagcccct ttctccaaat cttctacgtt ctatagaaac agcccagcaa
    14941 gggaattgca tcttcctata agggatggta atactttgga aatgcatggt gacacctgcc
    15001 aacctggcat tttcaactat gccacaaggc tgggaaggag aagcaagagt cctcaggcca
    15061 tggcatcaca tggttctaga ccagggagtc gcctaaagca gccgattggg cagattccac
    15121 tggaatcttc tcctccagtc ggactttcta ttgaagaagt ggagaggctc aacacacctc
    15181 gccctagaaa cccaagtatc tgcagtgcag accatgggag gtcttcttca gaggaggact
    15241 gcagaaggcc actgtctaga acaaggaatc cagcggatgg cattccagct ccagaatcct
    15301 cttctgatag tgactcccat gaatctttca cttgctcaga aatggaatat gacagggaga
    15361 agccaatggt atatacttcc aggatgccca aattatctca agtcaatgaa tctgatgcag
    15421 atgatgaaga taattatgga gccagactga agcctcgaag gtaccacggt cgcagggccg
    15481 agggaggacc tgtgggcacc caggcagcag caccaggcac tgctgacaac acactgccca
    15541 tgaagctagg gcagcaagca gggactttca actgggacaa ccttttgaac tggggccctg
    15601 gctttggcca ttatgtagat gtttttaaag atttggcatc tcttccagaa aaagcagcag
    15661 caaatgaaga aggcaaagct gggacaacta aaccagtccc caaagatggg gaagcagaac
    15721 agtatgtggt cgagggctct ggcatggtga gcaagggcga ggagctgttc accggggtgg
    15781 tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg
    15841 agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca
    15901 agctgcccgt gccctggccc accctcgtga ccaccttcgg ctacggcctg atgtgcttcg
    15961 cccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct
    16021 acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg
    16081 tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg
    16141 aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata
    16201 tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg
    16261 aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc
    16321 ccgtgctgct gcccgacaac cactacctga gctaccagtc cgccctgagc aaagacccca
    16381 acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg
    16441 gcatggacga gctgtacaag tgagggcccg aacaaaaact catctcagaa gaggatctga
    16501 atagcgccgt cgaccatcat catcatcatc attgagttta aacccgctga tcagcctcga
    16561 ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc
    16621 tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc
    16681 tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt
    16741 gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa
    16801 gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg
    16861 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
    16921 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
    16981 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
    17041 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
    17101 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
    17161 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
    17221 taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca
    17281 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
    17341 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
    17401 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
    17461 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
    17521 tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt
    17581 tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga
    17641 tcagcacgtg ttgacaatta atcatcggca tagtatatcg gcatagtata atacgacaag
    17701 gtgaggaact aaaccatggc caagttgacc agtgccgttc cggtgctcac cgcgcgcgac
    17761 gtcgccggag cggtcgagtt ctggaccgac cggctcgggt tctcccggga cttcgtggag
    17821 gacgacttcg ccggtgtggt ccgggacgac gtgaccctgt tcatcagcgc ggtccaggac
    17881 caggtggtgc cggacaacac cctggcctgg gtgtgggtgc gcggcctgga cgagctgtac
    17941 gccgagtggt cggaggtcgt gtccacgaac ttccgggacg cctccgggcc ggccatgacc
    18001 gagatcggcg agcagccgtg ggggcgggag ttcgccctgc gcgacccggc cggcaactgc
    18061 gtgcacttcg tggccgagga gcaggactga cacgtgctac gagatttcga ttccaccgcc
    18121 gccttctatg aaaggttggg cttcggaatc gttttccggg accccggctg gatgatcctc
    18181 cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat tgcagcttat
    18241 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
    18301 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg tataccgtcg
    18361 acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
    18421 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
    18481 taatgagtga gctaacttac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
    18541 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
    18601 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
    18661 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
    18721 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
    18781 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
    18841 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
    18901 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
    18961 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
    19021 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
    19081 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
    19141 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
    19201 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
    19261 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
    19321 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
    19381 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
    19441 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
    19501 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
    19561 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
    19621 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
    19681 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
    19741 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
    19801 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
    19861 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
    19921 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
    19981 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
    20041 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
    20101 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
    20161 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
    20221 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
    20281 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
    20341 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
    20401 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
    20461 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
    20521 tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atctcccgat cccctatggt
    20581 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
    20641 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
    20701 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
    20761 tacgggccag atatacgcgt t
//
