LOCUS       sequence-240993-        9524 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This plasmid encodes the complete CRISPR/dCas9 machinery for 
            repressing transposition of the following bacterial Insertion 
            Sequences: IS1, IS3 and IS5..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9524)
  AUTHORS   Nyerges A, Balint B, Cseklye J, Nagy I, Pal C, Feher T
  TITLE     CRISPR-interference-based modulation of mobile genetic elements in 
            bacteria.
  JOURNAL   Synth Biol (Oxf). 2019;4(1):ysz008. doi: 10.1093/synbio/ysz008. Epub
            2019 Mar 15.
  PUBMED    31008359
REFERENCE   2  (bases 1 to 9524)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2019"; volume: "4(1):ysz008. doi"; pages: " 
            10.1093/synbio/ysz008. Epub 2019 Mar 15"
COMMENT     Sequence Label: pCRISP_IS
FEATURES             Location/Qualifiers
     source          1..9524
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(291..317,318..320,321..2807,2808..2810,2811..4397)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     misc_feature    4418..4549
                     /label=crRNA leader
                     /note="crRNA leader sequence for the Streptococcus pyogenes
                     CRISPR/Cas system"
     repeat_region   4550..4585
                     /label=DR
                     /note="direct repeat for the Streptococcus pyogenes 
                     CRISPR/Cas system"
     repeat_region   4616..4651
                     /label=DR
                     /note="direct repeat for the Streptococcus pyogenes 
                     CRISPR/Cas system"
     repeat_region   4682..4717
                     /label=DR
                     /note="direct repeat for the Streptococcus pyogenes 
                     CRISPR/Cas system"
     repeat_region   4748..4783
                     /label=DR
                     /note="direct repeat for the Streptococcus pyogenes 
                     CRISPR/Cas system"
     repeat_region   4814..4849
                     /label=DR
                     /note="direct repeat for the Streptococcus pyogenes 
                     CRISPR/Cas system"
     primer_bind     complement(5278..5297)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     CDS             complement(7150..7809)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     7724..7743
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(7810..7912)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(8304..8321)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8438..8983)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(8518..8537)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        join(9095..9100,9101..9117,9118..9123)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     misc_RNA        complement(9441..9519)
                     /label=tracrRNA
                     /note="trans-activating CRISPR RNA for the Streptococcus 
                     pyogenes CRISPR/Cas9 system"
ORIGIN
        1 gattatttta taacttttat aacaaataat caaggagaaa ttcaaagaaa tttatcagcc
       61 ataaaacaat acttaatact atagaatgat aacaaaataa actacttttt aaaagaattt
      121 tgtgttataa tctatttatt attaagtatt gggtaatatt ttttgaagag atattttgaa
      181 aaagaaaaat taaagcatat taaactaatt tcggaggtca ttaaaactat tattgaaatc
      241 atcaaactca ttatggattt aatttaaact ttttatttta ggaggcaaaa atggataaga
      301 aatactcaat aggcttagct atcggcacaa atagcgtcgg atgggcggtg atcactgatg
      361 aatataaggt tccgtctaaa aagttcaagg ttctgggaaa tacagaccgc cacagtatca
      421 aaaaaaatct tataggggct cttttatttg acagtggaga gacagcggaa gcgactcgtc
      481 tcaaacggac agctcgtaga aggtatacac gtcggaagaa tcgtatttgt tatctacagg
      541 agattttttc aaatgagatg gcgaaagtag atgatagttt ctttcatcga cttgaagagt
      601 cttttttggt ggaagaagac aagaagcatg aacgtcatcc tatttttgga aatatagtag
      661 atgaagttgc ttatcatgag aaatatccaa ctatctatca tctgcgaaaa aaattggtag
      721 attctactga taaagcggat ttgcgcttaa tctatttggc cttagcgcat atgattaagt
      781 ttcgtggtca ttttttgatt gagggagatt taaatcctga taatagtgat gtggacaaac
      841 tatttatcca gttggtacaa acctacaatc aattatttga agaaaaccct attaacgcaa
      901 gtggagtaga tgctaaagcg attctttctg cacgattgag taaatcaaga cgattagaaa
      961 atctcattgc tcagctcccc ggtgagaaga aaaatggctt atttgggaat ctcattgctt
     1021 tgtcattggg tttgacccct aattttaaat caaattttga tttggcagaa gatgctaaat
     1081 tacagctttc aaaagatact tacgatgatg atttagataa tttattggcg caaattggag
     1141 atcaatatgc tgatttgttt ttggcagcta agaatttatc agatgctatt ttactttcag
     1201 atatcctaag agtaaatact gaaataacta aggctcccct atcagcttca atgattaaac
     1261 gctacgatga acatcatcaa gacttgactc ttttaaaagc tttagttcga caacaacttc
     1321 cagaaaagta taaagaaatc ttttttgatc aatcaaaaaa cggatatgca ggttatattg
     1381 atgggggagc tagccaagaa gaattttata aatttatcaa accaatttta gaaaaaatgg
     1441 atggtactga ggaattattg gtgaaactaa atcgtgaaga tttgctgcgc aagcaacgga
     1501 cctttgacaa cggctctatt ccccatcaaa ttcacttggg tgagctgcat gctattttga
     1561 gaagacaaga agacttttat ccatttttaa aagacaatcg tgagaagatt gaaaaaatct
     1621 tgacttttcg aattccttat tatgttggtc cattggcgcg tggcaatagt cgttttgcat
     1681 ggatgactcg gaagtctgaa gaaacaatta ccccatggaa ttttgaagaa gttgtcgata
     1741 aaggtgcttc agctcaatca tttattgaac gcatgacaaa ctttgataaa aatcttccaa
     1801 atgaaaaagt actaccaaaa catagtttgc tttatgagta ttttacggtt tataacgaat
     1861 tgacaaaggt caaatatgtt actgaaggaa tgcgaaaacc agcatttctt tcaggtgaac
     1921 agaagaaagc cattgttgat ttactcttca aaacaaatcg aaaagtaacc gttaagcaat
     1981 taaaagaaga ttatttcaaa aaaatagaat gttttgatag tgttgaaatt tcaggagttg
     2041 aagatagatt taatgcttca ttaggtacct accatgattt gctaaaaatt attaaagata
     2101 aagatttttt ggataatgaa gaaaatgaag atatcttaga ggatattgtt ttaacattga
     2161 ccttatttga agatagggag atgattgagg aaagacttaa aacatatgct cacctctttg
     2221 atgataaggt gatgaaacag cttaaacgtc gccgttatac tggttgggga cgtttgtctc
     2281 gaaaattgat taatggtatt agggataagc aatctggcaa aacaatatta gattttttga
     2341 aatcagatgg ttttgccaat cgcaatttta tgcagctgat ccatgatgat agtttgacat
     2401 ttaaagaaga cattcaaaaa gcacaagtgt ctggacaagg cgatagttta catgaacata
     2461 ttgcaaattt agctggtagc cctgctatta aaaaaggtat tttacagact gtaaaagttg
     2521 ttgatgaatt ggtcaaagta atggggcggc ataagccaga aaatatcgtt attgaaatgg
     2581 cacgtgaaaa tcagacaact caaaagggcc agaaaaattc gcgagagcgt atgaaacgaa
     2641 tcgaagaagg tatcaaagaa ttaggaagtc agattcttaa agagcatcct gttgaaaata
     2701 ctcaattgca aaatgaaaag ctctatctct attatctcca aaatggaaga gacatgtatg
     2761 tggaccaaga attagatatt aatcgtttaa gtgattatga tgtcgatgcc attgttccac
     2821 aaagtttcct taaagacgat tcaatagaca ataaggtctt aacgcgttct gataaaaatc
     2881 gtggtaaatc ggataacgtt ccaagtgaag aagtagtcaa aaagatgaaa aactattgga
     2941 gacaacttct aaacgccaag ttaatcactc aacgtaagtt tgataattta acgaaagctg
     3001 aacgtggagg tttgagtgaa cttgataaag ctggttttat caaacgccaa ttggttgaaa
     3061 ctcgccaaat cactaagcat gtggcacaaa ttttggatag tcgcatgaat actaaatacg
     3121 atgaaaatga taaacttatt cgagaggtta aagtgattac cttaaaatct aaattagttt
     3181 ctgacttccg aaaagatttc caattctata aagtacgtga gattaacaat taccatcatg
     3241 cccatgatgc gtatctaaat gccgtcgttg gaactgcttt gattaagaaa tatccaaaac
     3301 ttgaatcgga gtttgtctat ggtgattata aagtttatga tgttcgtaaa atgattgcta
     3361 agtctgagca agaaataggc aaagcaaccg caaaatattt cttttactct aatatcatga
     3421 acttcttcaa aacagaaatt acacttgcaa atggagagat tcgcaaacgc cctctaatcg
     3481 aaactaatgg ggaaactgga gaaattgtct gggataaagg gcgagatttt gccacagtgc
     3541 gcaaagtatt gtccatgccc caagtcaata ttgtcaagaa aacagaagta cagacaggcg
     3601 gattctccaa ggagtcaatt ttaccaaaaa gaaattcgga caagcttatt gctcgtaaaa
     3661 aagactggga tccaaaaaaa tatggtggtt ttgatagtcc aacggtagct tattcagtcc
     3721 tagtggttgc taaggtggaa aaagggaaat cgaagaagtt aaaatccgtt aaagagttac
     3781 tagggatcac aattatggaa agaagttcct ttgaaaaaaa tccgattgac tttttagaag
     3841 ctaaaggata taaggaagtt aaaaaagact taatcattaa actacctaaa tatagtcttt
     3901 ttgagttaga aaacggtcgt aaacggatgc tggctagtgc cggagaatta caaaaaggaa
     3961 atgagctggc tctgccaagc aaatatgtga attttttata tttagctagt cattatgaaa
     4021 agttgaaggg tagtccagaa gataacgaac aaaaacaatt gtttgtggag cagcataagc
     4081 attatttaga tgagattatt gagcaaatca gtgaattttc taagcgtgtt attttagcag
     4141 atgccaattt agataaagtt cttagtgcat ataacaaaca tagagacaaa ccaatacgtg
     4201 aacaagcaga aaatattatt catttattta cgttgacgaa tcttggagct cccgctgctt
     4261 ttaaatattt tgatacaaca attgatcgta aacgatatac gtctacaaaa gaagttttag
     4321 atgccactct tatccatcaa tccatcactg gtctttatga aacacgcatt gatttgagtc
     4381 agctaggagg tgactgaagt atattttaga tgaagattat ttcttaataa ctaaaaatat
     4441 ggtataatac tcttaataaa tgcagtaata caggggcttt tcaagactga agtctagctg
     4501 agacaaatag tgcgattacg aaatttttta gacaaaaata gtctacgagg ttttagagct
     4561 atgctgtttt gaatggtccc aaaacatgct gccaacttac tgatttagtg tatgagtttt
     4621 agagctatgc tgttttgaat ggtcccaaaa cggctccaga tgacaaacat gatctcatat
     4681 cgttttagag ctatgctgtt ttgaatggtc ccaaaacacg cggctaagtg agtaaactct
     4741 cagtcaggtt ttagagctat gctgttttga atggtcccaa aactgagacc tgtctcggaa
     4801 gctcatagga ctcgttttag agctatgctg ttttgaatgg tcccaaaact tcagcacact
     4861 gagacttgtt gagttccatg ttttagagct atgctgtttt gaatggactc cattcaacat
     4921 tgccgatgat aacttgagaa agagggttaa taccagcagt cggatacctt cctattcttt
     4981 ctgttaaagc gttttcatgt tataataggc aaaagaagag tagtgtgatc gtccattccg
     5041 acagcatcgc cagtcactat ggcgtgctgc tagcgctata tgcgttgatg caatttctat
     5101 gcgcacccgt tctcggagca ctgtccgacc gctttggccg ccgcccagtc ctgctcgctt
     5161 cgctacttgg agccactatc gactacgcga tcatggcgac cacacccgtc ctgtggatcc
     5221 tctacgccgg acgcatcgtg gccggcatca ccggcgccac aggtgcggtt gctggcgcct
     5281 atatcgccga catcaccgat ggggaagatc gggctcgcca cttcgggctc atgagcgctt
     5341 gtttcggcgt gggtatggtg gcaggccccg tggccggggg actgttgggc gccatctcct
     5401 tgcatgcacc attccttgcg gcggcggtgc tcaacggcct caacctacta ctgggctgct
     5461 tcctaatgca ggagtcgcat aagggagagc gtcgaccgat gcccttgaga gccttcaacc
     5521 cagtcagctc cttccggtgg gcgcggggca tgactatcgt cgccgcactt atgactgtct
     5581 tctttatcat gcaactcgta ggacaggtgc cggcagcgct ctgggtcatt ttcggcgagg
     5641 accgctttcg ctggagcgcg acgatgatcg gcctgtcgct tgcggtattc ggaatcttgc
     5701 acgccctcgc tcaagccttc gtcactggtc ccgccaccaa acgtttcggc gagaagcagg
     5761 ccattatcgc cggcatggcg gccgacgcgc tgggctacgt cttgctggcg ttcgcgacgc
     5821 gaggctggat ggccttcccc attatgattc ttctcgcttc cggcggcatc gggatgcccg
     5881 cgttgcaggc catgctgtcc aggcaggtag atgacgacca tcagggacag cttcaaggat
     5941 cgctcgcggc tcttaccagc ctaacttcga tcattggacc gctgatcgtc acggcgattt
     6001 atgccgcctc ggcgagcaca tggaacgggt tggcatggat tgtaggcgcc gccctatacc
     6061 ttgtctgcct ccccgcgttg cgtcgcggtg catggagccg ggccacctcg acctgaatgg
     6121 aagccggcgg cacctcgcta acggattcac cactccaaga attggagcca atcaattctt
     6181 gcggagaact gtgaatgcgc aaaccaaccc ttggcagaac atatccatcg cgtccgccat
     6241 ctccagcagc cgcacgcggc gcatctcggg cagcgttggg tcctggccac gggtgcgcat
     6301 gatcgtgctc ctgtcgttga ggacccggct aggctggcgg ggttgcctta ctggttagca
     6361 gaatgaatca ccgatacgcg agcgaacgtg aagcgactgc tgctgcaaaa cgtctgcgac
     6421 ctgagcaaca acatgaatgg tcttcggttt ccgtgtttcg taaagtctgg aaacgcggaa
     6481 gtcccctacg tgctgctgaa gttgcccgca acagagagtg gaaccaaccg gtgataccac
     6541 gatactatga ctgagagtca acgccatgag cggcctcatt tcttattctg agttacaaca
     6601 gtccgcaccg ctgccggtag ctccttccgg tgggcgcggg gcatgactat cgtcgccgca
     6661 cttatgactg tcttctttat catgcaactc gtaggacagg tgccggcagc gcccaacagt
     6721 cccccggcca cggggcctgc caccataccc acgccgaaac aagcgccctg caccattatg
     6781 ttccggatct gcatcgcagg atgctgctgg ctaccctgtg gaacacctac atctgtatta
     6841 acgaagcgct aaccgttttt atcaggctct gggaggcaga ataaatgatc atatcgtcaa
     6901 ttattacctc cacggggaga gcctgagcaa actggcctca ggcatttgag aagcacacgg
     6961 tcacactgct tccggtagtc aataaaccgg taaaccagca atagacataa gcggctattt
     7021 aacgaccctg ccctgaaccg acgaccgggt cgaatttgct ttcgaatttc tgccattcat
     7081 ccgcttatta tcacttattc aggcgtagca accaggcgtt taagggcacc aataactgcc
     7141 ttaaaaaaat tacgccccgc cctgccactc atcgcagtac tgttgtaatt cattaagcat
     7201 tctgccgaca tggaagccat cacaaacggc atgatgaacc tgaatcgcca gcggcatcag
     7261 caccttgtcg ccttgcgtat aatatttgcc catggtgaaa acgggggcga agaagttgtc
     7321 catattggcc acgtttaaat caaaactggt gaaactcacc cagggattgg ctgagacgaa
     7381 aaacatattc tcaataaacc ctttagggaa ataggccagg ttttcaccgt aacacgccac
     7441 atcttgcgaa tatatgtgta gaaactgccg gaaatcgtcg tggtattcac tccagagcga
     7501 tgaaaacgtt tcagtttgct catggaaaac ggtgtaacaa gggtgaacac tatcccatat
     7561 caccagctca ccgtctttca ttgccatacg gaattccgga tgagcattca tcaggcgggc
     7621 aagaatgtga ataaaggccg gataaaactt gtgcttattt ttctttacgg tctttaaaaa
     7681 ggccgtaata tccagctgaa cggtctggtt ataggtacat tgagcaactg actgaaatgc
     7741 ctcaaaatgt tctttacgat gccattggga tatatcaacg gtggtatatc cagtgatttt
     7801 tttctccatt ttagcttcct tagctcctga aaatctcgat aactcaaaaa atacgcccgg
     7861 tagtgatctt atttcattat ggtgaaagtt ggaacctctt acgtgccgat caacgtctca
     7921 ttttcgccaa aagttggccc ggggcttccc ggtatcaaca gggacaccag gatttattta
     7981 ttctgcgaag tgatcttccg tcacaggtat ttattcggcg caaagtgcgt cgggtgatgc
     8041 tgccaactta ctgatttagt gtatgatggt gtttttgagg tgctccagtg gcttctgttt
     8101 ctatcagctg tccctcctgt tcagctactg acggggtggt gcgtaacggc aaaagcaccg
     8161 ccggacatca gcgctagcgg agtgtatact ggcttactat gttggcactg atgagggtgt
     8221 cagtgaagtg cttcatgtgg caggagaaaa aaggctgcac cggtgcgtca gcagaatatg
     8281 tgatacagga tatattccgc ttcctcgctc actgactcgc tacgctcggt cgttcgactg
     8341 cggcgagcgg aaatggctta cgaacggggc ggagatttcc tggaagatgc caggaagata
     8401 cttaacaggg aagtgagagg gccgcggcaa agccgttttt ccataggctc cgcccccctg
     8461 acaagcatca cgaaatctga cgctcaaatc agtggtggcg aaacccgaca ggactataaa
     8521 gataccaggc gtttccccct ggcggctccc tcgtgcgctc tcctgttcct gcctttcggt
     8581 ttaccggtgt cattccgctg ttatggccgc gtttgtctca ttccacgcct gacactcagt
     8641 tccgggtagg cagttcgctc caagctggac tgtatgcacg aaccccccgt tcagtccgac
     8701 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggaaagaca tgcaaaagca
     8761 ccactggcag cagccactgg taattgattt agaggagtta gtcttgaagt catgcgccgg
     8821 ttaaggctaa actgaaagga caagttttgg tgactgcgct cctccaagcc agttacctcg
     8881 gttcaaagag ttggtagctc agagaacctt cgaaaaaccg ccctgcaagg cggttttttc
     8941 gttttcagag caagagatta cgcgcagacc aaaacgatct caagaagatc atcttattaa
     9001 tcagataaaa tatttctaga tttcagtgca atttatctct tcaaatgtag cacctgaagt
     9061 cagccccata cgatataagt tgtaattcta atgtttgaca gcttatcatc gataagcttt
     9121 aatgcggtag tttatcacag ttaaattgct aacgcagtca ggcaccgtgt atgaaatcta
     9181 acaatgcgct catcgtcatc ctcggcaccg tcaccctgga tgctgtaggc ataggcttgg
     9241 ttatgccggt actgccgggc ctcttgcggg attacgaaat catcctgtgg agcttagtag
     9301 gtttagcaag atggcagcgc ctaaatgtag aatgataaaa ggattaagag attaatttcc
     9361 ctaaaaatga taaaacaagc gttttgaaag cgcttgtttt tttggtttgc agtcagagta
     9421 gaatagaagt atcaaaaaaa gcaccgactc ggtgccactt tttcaagttg ataacggact
     9481 agccttattt taacttgcta tgctgttttg aatggttcca acaa
//
