LOCUS       sequence-240994-        3347 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BglFusion compatible IPTG inducible integration vector for 
            Streptococcus pneumoniae.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3347)
  AUTHORS   Keller LE, Rueff AS, Kurushima J, Veening JW
  TITLE     Three New Integration Vectors and Fluorescent Proteins for Use in 
            the Opportunistic Human Pathogen Streptococcus pneumoniae.
  JOURNAL   Genes (Basel). 2019 May 22;10(5). pii: genes10050394. doi: 
            10.3390/genes10050394.
  PUBMED    31121970
REFERENCE   2  (bases 1 to 3347)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes 
            (Basel)."; date: "2019-05-22"; volume: "10(5). pii: genes10050394. 
            doi"; pages: " 10.3390/genes10050394"
COMMENT     Sequence Label: pPEPX-Plac
FEATURES             Location/Qualifiers
     source          1..3347
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      203..274
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      290..317
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     protein_bind    414..433
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     rep_origin      1120..1708
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1609..1628
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gcatgcggca aagcactcaa aagtttacct tatgggtgct tttttcgtgc ttttttgaaa
       61 agacaaaaaa aagaaccttg ccaagcaaga ttcttttgat agggtggtct ccgatcgtgt
      121 actgcagaaa actagtcgca aaaaaccccg cttcggcggg gttttttcgc tctagacggt
      181 gatcaacacg ctagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt
      241 tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc
      301 gggtgggcct ttctgcgttt atagaattca ctagtaattg tgaggcggat aacaattctc
      361 actcattcta cagtttattc ttgacattgc actgtccccc tggtataata actaattgtg
      421 agcgctcaca attaagatct aaataaataa ggaggaaaat taatgccgga tccctccagt
      481 aactcgagaa aaaaaaaccg cgcccctgac agggcgcggt ttttttttaa gtggacacct
      541 acgtatggta actgtacagg tcgctgaaac ttctatcgtc aagaagaatc accaaatccc
      601 tcgtatcatc aaccaaaaga ttgctcaaaa gttaattgaa aagatttcta tgactgatat
      661 tgcccatcag ctttccatct caacttcaac tgttattcgt aagctcaatg attttcactt
      721 taaacatgat ttttcgcgtc ttcctgagat tatgtcttgg gatgagtatg cttttacaaa
      781 gggaaagatg agcttcattg cgcaagattt taacaatctc aatatcatca ctgttcttga
      841 gggaagaaca caagctatca ttcgaaatca ctttcttcgc tacgatagag ttgtccgatg
      901 tcgcgtcaaa attattacta tggatatgtt tagcccttac tatgacttgg ctaaacagct
      961 tcgctttcaa atttctaggc tcaggctgaa acagtctccc agactgtttc actcccgaat
     1021 gctaaaatcg ttcttgatcg ctttcacatt ggacgtcctg accatggagt caagtgtaag
     1081 gcactggtac caccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     1141 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     1201 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     1261 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     1321 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     1381 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     1441 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     1501 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     1561 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     1621 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     1681 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     1741 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccgcg gccgcagtta
     1801 aagaacttgg actgcgctct aaaaccaacg tggtcagtct ggacaagatt attattgata
     1861 aaaaatcctc actgataacc ggtttcccag agtttcggat ggtttggaag gtggtccgcc
     1921 agcgtgtggt ggatgatctg gtatccgttc tggatacgga ctatctggat atggaactaa
     1981 tcccaaatct cactcgccaa atcgctgagc agtctatcta ttcttatata gaaaatggcc
     2041 tcaaactcct tattgattat gctcagaagg aaatcaccat tgaccactca agcgaccgag
     2101 acaagattct catggacatt ggcaaagacc cttatgtcgt ttcgattaaa tcaaaagtct
     2161 atctccaaga cggacgccaa tttcagttta ccgaaagtcg ccataagtta gaaaaattta
     2221 gatttgtaga ttttgcaaaa cgcaagaaat aaaagactga gacaccagat catggctaga
     2281 aagcttctag caaaaaactg gacggagtcc cgtccagaaa agtaagcact tgttattgca
     2341 ataaaattag cctaattgag agaagtttct atagaatttt tcatatactt aacgagtgct
     2401 ttcacctttg aatatagtcc ttcccactta tcatcacact ctccccgata gccttttcta
     2461 gctatatcca gtaaagttac atgctcttta ggtaaaagag gtatagccca ttcagcagcg
     2521 acatctttcg aggtaatttc accagtagtc actgtttgcc acattcgagc tagggttaaa
     2581 attacattac gctcatcacc ttttatcccc tcaattagtt ctggcaaaga atccttaatt
     2641 gctcttcgaa tatctgtcaa aggtacggag acaagtatac ttgaagaatc aggaccaaat
     2701 agagaaatac tattctttct tgcttgtgct aaaacaatag ccaaatcagg atcatagctt
     2761 ggttcctgaa tttgtccatt ctcaaattca cccctgagcc actcaccgta tataaattct
     2821 ctttttggag gatattgcca agggacaact tcactcctat ttataaccgt aacttcaagt
     2881 ggtctaacag aatccgtatt tccaatcctt cctgatatag tcattagtct ttctgttagt
     2941 ttttttcgag ttaattgagg taaactatga ttcacgacga ctagaacatc tacatcgctg
     3001 ttaatgcgta aaccaccatt tactgctgaa ccaaatagat atactccaac tattgaactt
     3061 ccaaataaat cttttacgat ttttaatgtt tgaatcgctt gatttggtat ttttccgtta
     3121 atcaaattgc tcatgatttc acctcgttga ttatgttcat ataaagttta tattgatact
     3181 caatttactt accctagatt ggacatatac ttaaattact gttcaataaa gctgaccgtt
     3241 agcgtttaag tacatccttt cacaatttgt ctacagaaca gtactggcct acctagtata
     3301 gcattttgtg aagttttttt ctagtccaag ctcacaaaaa tccaaag
//
