LOCUS       sequence-240998-       10304 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of ARAF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10304)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 10304)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-ARAF
FEATURES             Location/Qualifiers
     source          1..10304
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2223..3401
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2454..3392
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3349..3369
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    3421..3445
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5264..5288)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        5296..5314
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5339..5358)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5340..5358)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(5364..5384)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             5438..5479
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    5500..6067
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    5517..6069
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5661..5678)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5888..5907
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6081..6680
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6081..6100)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6577..6597
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6694..7282
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6747..6767)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7165..7176)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7357..7590
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7709..7727)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7795..7899
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7900..7968,7969..8760)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8118..8137)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8931..9519
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9420..9439
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9673..9690
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9930..9951
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9966..9971,9972..9989,9990..9996)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10004..10020
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10009..10031
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10028..10044
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10028..10044
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10094..10113
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccct gcagatatca
     3421 acaagtttgt acaaaaaagt tggcatggag ccaccacggg gcccccctgc caatggggcc
     3481 gagccatccc gggcagtggg caccgtcaaa gtatacctgc ccaacaagca acgcacggtg
     3541 gtgactgtcc gggatggcat gagtgtctac gactctctag acaaggccct gaaggtgcgg
     3601 ggtctaaatc aggactgctg tgtggtctac cgactcatca agggacgaaa gacggtcact
     3661 gcctgggaca cagccattgc tcccctggat ggcgaggagc tcattgtcga ggtccttgaa
     3721 gatgtcccgc tgaccatgca caattttgta cggaagacct tcttcagcct ggcgttctgt
     3781 gacttctgcc ttaagtttct gttccatggc ttccgttgcc aaacctgtgg ctacaagttc
     3841 caccagcatt gttcctccaa ggtccccaca gtctgtgttg acatgagtac caaccgccaa
     3901 cagttctacc acagtgtcca ggatttgtcc ggaggctcca gacagcatga ggctccctcg
     3961 aaccgccccc tgaatgagtt gctaaccccc cagggtccca gcccccgcac ccagcactgt
     4021 gacccggagc acttcccctt ccctgcccca gccaatgccc ccctacagcg catccgctcc
     4081 acgtccactc ccaacgtcca tatggtcagc accacggccc ccatggactc caacctcatc
     4141 cagctcactg gccagagttt cagcactgat gctgccggta gtagaggagg tagtgatgga
     4201 accccccggg ggagccccag cccagccagc gtgtcctcgg ggaggaagtc cccacattcc
     4261 aagtcaccag cagagcagcg cgagcggaag tccttggccg atgacaagaa gaaagtgaag
     4321 aacctggggt accgggactc aggctattac tgggaggtac cacccagtga ggtgcagctg
     4381 ctgaagagga tcgggacggg ctcgtttggc accgtgtttc gagggcggtg gcatggcgat
     4441 gtggccgtga aggtgctcaa ggtgtcccag cccacagctg agcaggccca ggctttcaag
     4501 aatgagatgc aggtgctcag gaagacgcga catgtcaaca tcttgctgtt tatgggcttc
     4561 atgacccggc cgggatttgc catcatcaca cagtggtgtg agggctccag cctctaccat
     4621 cacctgcatg tggccgacac acgcttcgac atggtccagc tcatcgacgt ggcccggcag
     4681 actgcccagg gcatggacta cctccatgcc aagaacatca tccaccgaga tctcaagtct
     4741 aacaacatct tcctacatga ggggctcacg gtgaagatcg gtgactttgg cttggccaca
     4801 gtgaagactc gatggagcgg ggcccagccc ttggagcagc cctcaggatc tgtgctgtgg
     4861 atggcagctg aggtgatccg tatgcaggac ccgaacccct acagcttcca gtcagacgtc
     4921 tatgcctacg gggttgtgct ctacgagctt atgactggct cactgcctta cagccacatt
     4981 ggctgccgtg accagattat ctttatggtg ggccgtggct atctgtcccc ggacctcagc
     5041 aaaatctcca gcaactgccc caaggccatg cggcgcctgc tgtctgactg cctcaagttc
     5101 cagcgggagg agcggcccct cttcccccag atcctggcca caattgagct gctgcaacgg
     5161 tcactcccca agattgagcg gagtgcctcg gaaccctcct tgcaccgcac ccaggccgat
     5221 gagttgcctg cctgcctact cagcgcagcc cgccttgtgc cttacccaac tttcttgtac
     5281 aaagtggtga tatccaatta accctcacta aaggaaatta gttgaaagta tgagaaagcc
     5341 ctatagtgag tcgtattacc acccaacttt tctatacaaa gtggttgata tccagcacag
     5401 tggcggccgc tcgagtctag agggcccgcg gttcgaaggt aagcctatcc ctaaccctct
     5461 cctcggtctc gattctacgc gtaccggtta gtaatgagat ccctcccccc cccctaacgt
     5521 tactggccga agccgcttgg aataaggccg gtgtgcgttt gtctatatgt tattttccac
     5581 catattgccg tcttttggca atgtgagggc ccggaaacct ggccctgtct tcttgacgag
     5641 cattcctagg ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa
     5701 ggaagcagtt cctctggaag cttcttgaag acaaacaacg tctgtagcga ccctttgcag
     5761 gcagcggaac cccccacctg gcgacaggtg cctctgcggc caaaagccac gtgtataaga
     5821 tacacctgca aaggcggcac aaccccagtg ccacgttgtg agttggatag ttgtggaaag
     5881 agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg aaggatgccc agaaggtacc
     5941 ccattgtatg ggatctgatc tggggcctcg gtgcacatgc tttacatgtg tttagtcgag
     6001 gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg gttttccttt gaaaaacacg
     6061 atgataatat ggccacacat atgaccgagt acaagcccac ggtgcgcctc gccacccgcg
     6121 acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc
     6181 gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc
     6241 tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg
     6301 cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc
     6361 gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg
     6421 cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc
     6481 accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg
     6541 ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc
     6601 tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga
     6661 cccgcaagcc cggtgcctaa atcgatagat cctaatcaac ctctggatta caaaatttgt
     6721 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     6781 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     6841 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     6901 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     6961 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     7021 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     7081 tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc
     7141 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     7201 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     7261 tccctttggg ccgcctcccc gcctgagatc ctttaagacc aatgacttac aaggcagctg
     7321 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac
     7381 gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct
     7441 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     7501 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     7561 ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt
     7621 atttataact tgcaaagaaa tgaatatcag agagtgagag gcccgggtta attaaggaaa
     7681 gggctagatc attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat
     7741 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
     7801 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     7861 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     7921 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     7981 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     8041 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     8101 agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag
     8161 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     8221 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     8281 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     8341 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     8401 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     8461 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     8521 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     8581 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     8641 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     8701 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     8761 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     8821 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     8881 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     8941 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     9001 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     9061 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     9121 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     9181 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     9241 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     9301 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     9361 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     9421 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     9481 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     9541 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     9601 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     9661 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
     9721 cctctccccg cgcgttggcc gattcattaa tgcagcaagc tcatggctga ctaatttttt
     9781 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag
     9841 gcttttttgg aggcctaggc ttttgcaaaa agctccccgt ggcacgacag gtttcccgac
     9901 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
     9961 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
    10021 tttcacacag gaaacagcta tgacatgatt acgaatttca caaataaagc atttttttca
    10081 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatcaac
    10141 tggataactc aagctaacca aaatcatccc aaacttccca ccccataccc tattaccact
    10201 gccaattacc tgtggtttca tttactctaa acctgtgatt cctctgaatt attttcattt
    10261 taaagaaatt gtatttgtta aatatgtact acaaacttag tagt
//
