LOCUS       sequence-24100-e        1142 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1142)
  TITLE     T4 Lysozyme Plasmids - Tray 2
REFERENCE   2  (bases 1 to 1142)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: T4 Lysozyme A42S WT*
FEATURES             Location/Qualifiers
     source          1..1142
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    722..738
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(746..752,753..768,769..774))
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    817..833
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(841..847,848..863,864..869))
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
ORIGIN
        1 ctangaaaag cccgctcatt aggcgggctc agatctgctc atgtttgaca gcttatcatc
       61 gataagcttc ggcaataaat tcttgatatg ttttcatatg tttcctttaa atattttaat
      121 tagtaattgt ctattcaagt aattgtgaat atactatcac aattccaaga gaaagtaaac
      181 agctttatag atttttatac gcgtcccaag tgccagttct aaacgttgta atgactcgtt
      241 ttgcgcgatt aggtgtttga ttataccatc tacttttagc taagttaact gctgcttcat
      301 cccagcgttt ttgttgaagc atacgtaaag agttagtaaa tcctgccaca ccggtttctc
      361 ccatttggaa aaccatatta atcaatgcag cgcgacgaac cgcatcaaga gaatcataaa
      421 ccggttttaa tttagcattt ctcagaattc cgcgaacagc agcatcaaca tcctgattaa
      481 agagtttttc agcctcatct tttgtaatta caccattagt attacgccca atagctttat
      541 ctaattcaga tttagaagca ttaagtgatg gactttttgt aagcaaatga ccgatgccaa
      601 tagtgtaata gccttctgtg tctttataga ttttaagtct aagaccttca tctatacgta
      661 acatttcaaa tatattcata atacctccta agcatcgatg gatcctgttt cctgtgtgaa
      721 attgttatcc gctcacaatt ccacacatta tacgagccga tgattaattg tcaacagggg
      781 gatggggagt aagctgatcc tgtttcctgt gtgaaattgt tatccgctca caattccaca
      841 cattatacga gccgatgatt aattgtcaac agggggatgg ggagtaagct catcgatgga
      901 tcgatccggt ttcctggggg gaaatggtta tcccgcctcc cnaattcccc cncatttana
      961 cngagccggg angcanaaag nngtaaagcc cngggggggc cnnaangggg gancnnaacn
     1021 ttacnttnaa ttggggntgg cgcncncngg cccggctttn ccagncnggg aaaccnngnc
     1081 gggccnggga nnccatccga aagggggcna aaaccnttnc cngggnangg nnngnaannn
     1141 nc
//
