LOCUS       sequence-241001-        7395 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Chromatin Assembly Factor 1, Excitation: 590, 
            Emission: 649.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7395)
  TITLE     Davidson Red fluorescent proteins
REFERENCE   2  (bases 1 to 7395)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mPlum-CAF1-C-10
FEATURES             Location/Qualifiers
     source          1..7395
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        92..395
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        396..599
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     550..570
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             644..1321
                     /codon_start=1
                     /product="far-red monomeric derivative of DsRed fluorescent
                     protein (Wang et al., 2004)"
                     /label=mPlum
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKEHMEGSVNGHEFEIEGEGEGRPYEGTQTA
                     RLKVTKGGPLPFAWDILSPQIMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGEFIYKVKVRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEMK
                     MRLRLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRH
                     STGA"
     primer_bind     1045..1064
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     1239..1262
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     protein_bind    3140..3151
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     polyA_signal    4214..4335
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4251..4270)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4305..4324
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(4342..4797)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4479..4500)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4691..4710
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4824..4928
                     /gene="bla"
                     /label=AmpR promoter
     promoter        4930..5287
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4930..5121
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4953..4973)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5138..5273
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5322..6116
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             5322..6116
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5376..5395)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5986..6005
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(6304..6323)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    6348..6395
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6724..7312
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7213..7232
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttctgtggat aaccgtatta ccgccatgca ttagttatta atagtaatca attacggggt
       61 cattagttca tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc
      121 ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag
      181 taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc
      241 acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg
      301 gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc
      361 agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca
      421 atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca
      481 atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg
      541 ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctg
      601 gtttagtgaa ccgtcagatc cgctagcgct accggtggct accatggtga gcaagggcga
      661 ggaggtcatc aaggagttca tgcgcttcaa ggagcacatg gagggctccg tgaacggcca
      721 cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccag
      781 gctgaaggtg accaagggtg gccccctgcc cttcgcctgg gacatcctgt cccctcagat
      841 catgtacggc tccaaggcct acgtgaagca ccccgccgac atccccgact acttgaagct
      901 gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt
      961 gaccgtgacc caggactcct ccctgcagga cggcgagttc atctacaagg tgaaggtgcg
     1021 cggcaccaac ttcccctccg acggccccgt aatgcagaag aagaccatgg gctgggaggc
     1081 ctcctccgag cggatgtacc ccgaggacgg cgccctgaag ggcgagatga agatgaggct
     1141 gaggctgaag gacggcggcc actacgacgc cgaggtcaag accacctaca tggccaagaa
     1201 gcccgtgcag ctgcccggcg cctacaagac cgacatcaag ctggacatca cctcccacaa
     1261 cgaggactac accatcgtgg aacagtacga gcgcgccgag ggccgccact ccaccggcgc
     1321 ctccggactc agatctggca gcggtggagg cttggaggag ccggaggccg cgacgcggac
     1381 agccgcggcc gtggattgca aagacagacc cgggttccca gtgaagaggt taatacaagc
     1441 ccgcttgccc ttcaaacgcc tgaaccttgt tcccaaggag aaagttgagg aagacacatc
     1501 accaaaagca gctgtggaaa gcaaagtccc ggatctacaa ttgtctttgg gcacttttga
     1561 aagccagtgt cacacaggct ctcacgtagg cttgagtaca aagttggtcg gtggccaggg
     1621 ccccattgat agcttcttga gggcaacaat caagccagta ccgagtgtgg tcattatcga
     1681 cctgacggag aactgcagtg acattccaga cagccctgag ggccacagtg aactaagtcc
     1741 tgacactgct ggtgtggtca ccacagtgga aggagctgcc aagcagcagg agcactctgc
     1801 agcagagctg tgtctcctag agaccccttc agacatcact tgtcacatgg aagaggagcc
     1861 tgggagtcca ggagacccaa agaggacggg tgactgtcag gctggttcac tacagagctg
     1921 tccggagctg accccaggtt ctaggacatg ccccaccaag gagttatcaa gctggagcaa
     1981 ggcaggggat ctcctattta tagagaaagt gcctgtggtg gtgctagagg acatcctggc
     2041 cactaagccc tccattgcct ctcttcccat gatgtccctg gataggagtg tcacttcaga
     2101 gagtgaaata ctggagtcct gccccgagga tgactccata ctgagccact cctccaccaa
     2161 ctcctcttct ccaaccagct ctcctgaggg accatccaca cccccagaac atcggggtgg
     2221 gagaagctcc ccctccaccc cagcctgcag agttgccaag aactttgtca aaggctccac
     2281 agagaagggc aggagcaaac tgcacagaga tagagaacag cagagggaag agaaggaaaa
     2341 gctgagggaa gagatacgac gagccaagga ggaggcacgg aagaagaagg aggaggagaa
     2401 ggagctgaag gaaaaggagc ggagggaaaa gcgggagaaa gatgagaagg agaaggcgga
     2461 gaagcagcgg ctaaaggagg aaaaacgcaa ggagcggcag gaagctctgg aggctaaact
     2521 ggaggagaag aggaagaagg aggaagagaa gcggttacgg gaggaagaga agcggttacg
     2581 ggaagaagag aagcgcatta aggcagaaaa ggcagagatc actcgattct tccagaaacc
     2641 gaagactccg caggctccca agaccctggc cggttcctgt ggtaagtttg ccccctttga
     2701 aatcaaagaa cacatggtgc tggctccacg gtgccgagct gccttggatc aagacctgtg
     2761 cgatcagctg gaccagctcc tgcagcagca gagtgtggca agcaccttcc tcagtgacct
     2821 aaaaagccgg ctgcccttga gatcggggcc cacccgggtc tgtggccatg acacagacat
     2881 catgaacagg gatgtggtca ttgtggagag cagcaaggtg gacggtgtgt ctgaaaggaa
     2941 gaagtttggc cgcatgaagc ttctgcagtt ctcagagaac caccggccag catactgggg
     3001 gacgtggaac aagaagacag ccatcatccg cccccggaac ccctgggccc aggacaaaga
     3061 ccttcttgac tatgaggtag acagtgacga tgagtgggaa gaggaggaac ctggggagtc
     3121 tttgtcccac agtgaggggg atgaggatga cgatgtgggg gaagatgagg atgaggacga
     3181 tggcttcttt gtgccccatg ggtacttatc tgaagatgaa ggcgtgactg aggagtgtgc
     3241 agaccctgag aaccacaaag tacaccagaa gctgaaggcc aaggagtggg atgagctcct
     3301 agccaagggc aagcgctttc gtgtgctgca gcctgtgcac gtgggatgtg tatgggcatc
     3361 agaggcagcc aactgcacga gttccgactt gaagctgctg cagcagttca ctgcatgcct
     3421 gctggatgtg gcatcccctg atgagccaga acccggggcc tccaggaggg aaaagaggga
     3481 ccagcatatc ctggcccagt tgcttccgct gctacatggc aatgtgaatg ggagcaaggt
     3541 gatcatccat gaattccagg agcaatgccg caggggcctg ctgacccttc caagtcccac
     3601 cccacacctg cagatgccca acttggagga tgctgttgct gtgccatcca aggccaggct
     3661 gaagcgcctt atctctgaga actctgccta tgagaagcgg cccaacttcc gtatgtgctg
     3721 gtatgtgcac ccagaggtgc tcaagagctt tggccaggag tgcctgccag tgccctgcca
     3781 gtggacctac atcaccacca tgccctcagc acccagggaa gacagtggca gtgcttctac
     3841 tgaggggcct ggccagagca cacctatgct actcaagcgg aagccagcgg ccaccatgtg
     3901 catcacgcag tttatgaaga agcggcgcta tgatggacag gttgggtctg gggacatgga
     3961 tggcttccag gcagacacag aagaggatga agaggatgac acagactgta tgatcataga
     4021 tgtgccagat gttgggagtg acgtgtcaga agctcccata cctgctccca cactttgcaa
     4081 gtgaggatcc accggatcta gataactgat cataatcagc cataccacat ttgtagaggt
     4141 tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc
     4201 aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     4261 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     4321 catcaatgta tcttaacgcg taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa
     4381 atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata
     4441 aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac
     4501 tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc
     4561 cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa
     4621 atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg
     4681 cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg
     4741 tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtcag
     4801 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     4861 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     4921 ggaagagtcc tgaggcggaa agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa
     4981 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     5041 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     5101 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     5161 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     5221 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
     5281 ttgcaaagat cgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat
     5341 tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac
     5401 agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc
     5461 tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaagacgag gcagcgcggc
     5521 tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag
     5581 cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc
     5641 ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg
     5701 atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc
     5761 ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc
     5821 cagccgaact gttcgccagg ctcaaggcga gcatgcccga cggcgaggat ctcgtcgtga
     5881 cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca
     5941 tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg
     6001 atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg
     6061 ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg
     6121 gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga
     6181 ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg
     6241 gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccta gggggaggct
     6301 aactgaaaca cggaaggaga caataccgga aggaacccgc gctatgacgg caataaaaag
     6361 acagaataaa acgcacggtg ttgggtcgtt tgttcataaa cgcggggttc ggtcccaggg
     6421 ctggcactct gtcgataccc caccgagacc ccattggggc caatacgccc gcgtttcttc
     6481 cttttcccca ccccaccccc caagttcggg tgaaggccca gggctcgcag ccaacgtcgg
     6541 ggcggcaggc cctgccatag cctcaggtta ctcatatata ctttagattg atttaaaact
     6601 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     6661 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     6721 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     6781 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     6841 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     6901 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     6961 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     7021 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     7081 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     7141 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     7201 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     7261 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     7321 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     7381 tgcgttatcc cctga
//
