LOCUS       sequence-241006-       13076 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of BAI3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13076)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 13076)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-BAI3
FEATURES             Location/Qualifiers
     source          1..13076
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2223..3401
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2454..3392
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3349..3369
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    3421..3445
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(8013..8037)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             8090..8131
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    8152..8719
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    8169..8721
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(8313..8330)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     8540..8559
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(8733..8735,8736..8738,8739..9452)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(8733..8735,8736..8738,8739..9449)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8778..8799)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(9039..9058)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     9386..9407
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    9466..10054
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9519..9539)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9937..9948)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             10129..10362
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(10481..10499)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10567..10671
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10672..10740,10741..11532)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10890..10909)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11703..12291
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12192..12211
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12445..12462
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12702..12723
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12738..12743,12744..12761,12762..12768)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12776..12792
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12781..12803
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12800..12816
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12800..12816
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12866..12885
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccct gcagatatca
     3421 acaagtttgt acaaaaaagt tggcatgaag gctgttcgta acctgctgat ttatatattt
     3481 tccacctatc tcctggttat gtttggattt aatgctgccc aagacttctg gtgttcaact
     3541 ttggtgaagg gagtcattta tggatcgtat tctgtaagtg aaatgtttcc taaaaacttt
     3601 acaaactgca cttggacgct ggaaaatcca gatccaacca aatatagcat ttacctgaaa
     3661 ttttccaaaa aggaccttag ctgctctaac ttttcactcc tggcttatca gtttgatcat
     3721 ttttcccatg aaaaaataaa ggatctttta agaaagaatc attctataat gcaactctgc
     3781 aattccaaga atgctttcgt ttttctacag tatgataaaa attttattca aatacgtcga
     3841 gtatttccaa ctaatttccc aggattacag aaaaaagggg aagaagatca gaaatctttt
     3901 tttgagtttt tggtattgaa caaggtcagc ccaagccagt ttggttgcca tgtattatgt
     3961 acttggttgg agagctgctt aaaatcagaa aatgggagaa cagaatcatg tgggatcatg
     4021 tatacaaaat gcacctgccc tcagcatttg ggagagtggg ggatcgacga ccagtcgctg
     4081 attttgttaa ataacgtggt gttacccctg aatgagcaga cagagggctg cctgacccag
     4141 gagctgcaaa ccacccaagt ctgcaatctt accagggagg ccaagcgacc acccaaagaa
     4201 gaatttggaa tgatgggaga tcatacaatt aaaagtcagc gacctcgatc tgttcatgaa
     4261 aaaagggtcc ctcaggaaca agctgatgct gctaaattta tggcacaaac tggtgaatct
     4321 ggtgtggaag agtggtccca gtggagcaca tgttcggtta cttgtggtca agggtcgcag
     4381 gtgcgaacca gaacttgtgt atcaccttac gggacacact gcagcggccc attaagagaa
     4441 tcaagggttt gcaataacac tgccctctgt ccagtacacg gagtatggga ggaatggtca
     4501 ccatggagtt tatgttcatt tacatgtggt cgaggccaaa gaacaagaac aaggtcatgc
     4561 acacctcctc agtatggagg aaggccgtgt gaaggacctg aaacacatca taagccttgt
     4621 aatattgctc tttgcccagt tgatggacag tggcaagagt ggagttcgtg gagccagtgc
     4681 tcagtaacgt gctcgaatgg gactcagcag agaagccggc agtgcactgc agctgcccat
     4741 ggaggctccg aatgcagagg gccatgggca gaaagcagag agtgctataa ccctgaatgt
     4801 acagccaatg gtcaatggaa tcagtggggt cattggagtg gttgttccaa gtcctgtgat
     4861 ggcggctggg aaaggcgaat aaggacctgt cagggtgcag tgataacagg gcagcaatgt
     4921 gaaggaacgg gcgaagaagt gagaagatgc agtgagcagc gatgccctgc accttatgaa
     4981 atatgccctg aggattatct gatgtcgatg gtgtggaaaa gaactccagc aggcgacttg
     5041 gcattcaatc aatgtcccct gaatgccaca ggcaccacta gcagacgctg ctctctcagt
     5101 cttcatggag tggccttctg ggaacagccg agctttgcaa gatgcatatc aaatgagtac
     5161 agacacttgc agcattcaat taaagagcac cttgctaagg ggcagcgaat gctggcaggt
     5221 gatggaatgt cccaggtgac caagacactg ttggatttaa ctcagagaaa aaatttctat
     5281 gcaggcgatc ttctgatgtc tgtggagatc ctgagaaatg tgacagacac atttaaaagg
     5341 gcaagttaca tccctgcatc tgatggtgtc cagaacttct ttcaaatagt tagcaacctt
     5401 ctagatgaag aaaacaagga aaaatgggaa gatgcacaac agatttatcc agggtcaata
     5461 gagttaatgc aggtgattga agattttata cacattgttg gaatggggat gatggacttt
     5521 cagaattcat acttaatgac tggaaatgta gtggctagta ttcagaagct tcctgcagcc
     5581 tctgttctaa cagacatcaa ctttccaatg aaaggacgga agggaatggt tgactgggca
     5641 agaaactcag aagatagggt agtaattcca aaaagcattt tcactccggt gtcatcaaaa
     5701 gaattagatg aatcatctgt atttgttctt ggcgcagtcc tatacaaaaa cttagatcta
     5761 attttgccca ctttgagaaa ttatactgtc attaattcca aaatcatcgt ggtcacaata
     5821 aggcctgaac ccaaaacaac cgattcgttt ctggagatag aactagctca tttggctaat
     5881 ggtactttga atccctattg tgtattgtgg gatgactcca aaacgaacga gtctttggga
     5941 acgtggtcca cccagggatg taaaactgtg cttaccgatg catcccatac gaaatgctta
     6001 tgtgatcgtc tctctacctt cgccattttg gctcagcaac ctagagaaat aatcatggaa
     6061 tcctctggca caccttcagt taccctaata gtaggcagtg gtctttcttg cttggccttg
     6121 attaccctag cagttgtcta tgcagcatta tggaggtaca tacgctctga gagatccata
     6181 atactaatta acttctgcct gtctatcatc tcatccaata tcctcatact ggttggacag
     6241 actcagacac ataataagag tatctgcaca accaccactg catttttgca ctttttcttc
     6301 ctggcttcat tctgttgggt tttgactgag gcgtggcaat catatatggc tgtaactgga
     6361 aaaattagga cacggcttat aagaaaacgc tttttgtgcc ttggatgggg tttaccagca
     6421 ttagtagtgg ccacatcagt aggcttcacc agaacaaaag gatatggcac tgatcactac
     6481 tgctggctct ctcttgaagg aggactactc tatgcttttg tgggacctgc agccgctgtt
     6541 gtcctggtca acatggtgat tggcattttg gtatttaata aacttgtttc cagagatgga
     6601 atcctagata aaaagctcaa acacagagcc ggtcagatga gtgagcctca tagcggtttg
     6661 acgctcaaat gtgccaagtg tggagtagtt tcaacaacag ctttgtcagc caccaccgcc
     6721 agtaacgcca tggcgtctct ttggagctcc tgtgtggtgt tgccccttct ggctttgacg
     6781 tggatgtctg cggttctggc catgacagat aaacgctcca tattgtttca aatacttttt
     6841 gctgtgtttg attcattgca aggctttgtt atagtcatgg tccactgcat tcttcggaga
     6901 gaggttcagg atgcatttag atgccgattg agaaactgtc aggatcccat caatgcagat
     6961 tcttcgagtt cgtttcctaa tgggcatgct caaatcatga cagactttga aaaggatgta
     7021 gacattgcct gtcgatcagt tcttcataag gatattggtc cttgccgagc agccacaata
     7081 acaggaacac tttctaggat ttctctaaat gatgatgaag aagaaaaggg aacaaaccct
     7141 gaagggctaa gctattcaac attgcctgga aatgtcattt ccaaagtcat catccagcaa
     7201 cccacaggtt tgcacatgcc catgagtatg aatgagctta gcaatccatg tttgaaaaaa
     7261 gaaaatagtg aattgcggag aactgtgtac ttatgtacgg atgataattt gagaggggct
     7321 gacatggaca tagtccatcc tcaagaaaga atgatggaaa gtgactatat tgtgatgccc
     7381 agaagttctg taaataacca gccttcaatg aaagaagaaa gcaaaatgaa tattggcatg
     7441 gaaaccttgc cacatgaaag gctattgcac tacaaagtaa accctgaatt caatatgaat
     7501 ccccctgtaa tggaccagtt caatatgaac ttagagcaac atctcgcacc ccaggaacat
     7561 atgcagaatt tgccctttga acctcgcaca gctgtgaaga atttcatggc ctctgagttg
     7621 gatgataatg caggactatc aagaagtgaa actggatcaa cgatatcaat gagttcttta
     7681 gagagaagaa aatcacgata ttcagacctt gactttgaga aggtcatgca tacaaggaag
     7741 aggcatatgg aactatttca agaactaaat cagaaatttc aaactttgga cagatttcgg
     7801 gatataccaa atacaagcag tatggaaaac cccgcaccaa acaagaatcc atgggacact
     7861 ttcaaaaacc ccagtgaata cccgcattac accacaatca atgtcttaga cacagaggca
     7921 aaggatgctt tggaactgag gccagcagag tgggagaagt gtctgaattt gcctctggat
     7981 gtgcaagagg gtgactttca aacagaagtt taacccaact ttcttgtaca aagtggttga
     8041 tatccagcac agtggcggcc gctcgagtct agagggcccg cggttcgaag gtaagcctat
     8101 ccctaaccct ctcctcggtc tcgattctac gcgtaccggt tagtaatgag atccctcccc
     8161 cccccctaac gttactggcc gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat
     8221 gttattttcc accatattgc cgtcttttgg caatgtgagg gcccggaaac ctggccctgt
     8281 cttcttgacg agcattccta ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt
     8341 gaatgtcgtg aaggaagcag ttcctctgga agcttcttga agacaaacaa cgtctgtagc
     8401 gaccctttgc aggcagcgga accccccacc tggcgacagg tgcctctgcg gccaaaagcc
     8461 acgtgtataa gatacacctg caaaggcggc acaaccccag tgccacgttg tgagttggat
     8521 agttgtggaa agagtcaaat ggctctcctc aagcgtattc aacaaggggc tgaaggatgc
     8581 ccagaaggta ccccattgta tgggatctga tctggggcct cggtgcacat gctttacatg
     8641 tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac cacggggacg tggttttcct
     8701 ttgaaaaaca cgatgataat atggccacac atatggtgag caagggcgag gagctgttca
     8761 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg
     8821 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca
     8881 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc
     8941 agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc
     9001 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc
     9061 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg
     9121 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca
     9181 acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc
     9241 acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg
     9301 gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca
     9361 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga
     9421 tcactctcgg catggacgag ctgtacaagt aaatcgatag atcctaatca acctctggat
     9481 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     9541 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     9601 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
     9661 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
     9721 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     9781 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     9841 tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc
     9901 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
     9961 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
    10021 acgagtcgga tctccctttg ggccgcctcc ccgcctgaga tcctttaaga ccaatgactt
    10081 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa
    10141 ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc
    10201 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
    10261 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
    10321 gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc
    10381 ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag aggcccgggt
    10441 taattaagga aagggctaga tcattcttga agacgaaagg gcctcgtgat acgcctattt
    10501 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga
    10561 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
    10621 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
    10681 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
    10741 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
    10801 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
    10861 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac
    10921 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
    10981 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
    11041 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
    11101 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
    11161 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
    11221 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
    11281 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
    11341 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
    11401 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
    11461 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
    11521 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
    11581 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
    11641 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
    11701 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
    11761 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
    11821 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
    11881 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
    11941 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
    12001 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
    12061 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
    12121 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
    12181 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
    12241 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
    12301 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
    12361 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
    12421 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
    12481 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagcaa gctcatggct
    12541 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
    12601 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccc gtggcacgac
    12661 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact
    12721 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg
    12781 agcggataac aatttcacac aggaaacagc tatgacatga ttacgaattt cacaaataaa
    12841 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
    12901 gtctggatca actggataac tcaagctaac caaaatcatc ccaaacttcc caccccatac
    12961 cctattacca ctgccaatta cctgtggttt catttactct aaacctgtga ttcctctgaa
    13021 ttattttcat tttaaagaaa ttgtatttgt taaatatgta ctacaaactt agtagt
//
