LOCUS       sequence-241008-        9250 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of ZIKV protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9250)
  TITLE     Rehwinkel Lab plasmids
REFERENCE   2  (bases 1 to 9250)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLenti6.3/Envelope
FEATURES             Location/Qualifiers
     source          1..9250
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    510..528
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    531..549
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    590..614
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1540..1551
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(2169..2193)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2246..2287
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2311..2899
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2364..2384)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2782..2793)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        2914..3230
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3081..3216
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        3278..3325
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             3344..3742
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             3829..4062
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4134..4255
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4171..4190)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4225..4244
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4295..4430
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4450..4469)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4451..4469)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4479..4496)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4479..4495)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4488..4510)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4637..5092
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4724..4743)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4934..4955
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5118..5222
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5223..5291,5292..6083)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5441..5460)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6254..6842
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6743..6762
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6996..7013
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7130..7151
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7166..7171,7172..7189,7190..7196)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7204..7220
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7209..7231
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7228..7244
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7228..7244
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7263..7283
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7265..7283
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7311..7537
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7538..7718
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7765..7890
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8383..8616
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8801..8845
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9112..9229
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 gcttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc tccctatcag
      541 tgatagagat cgtcgactag tccagtgtgg tggaattctg cagatatcaa caagtttgta
      601 caaaaaagca ggctccgaat tcgcccttag gaggacagct atgatcaggt gcataggagt
      661 cagcaatagg gactttgtgg aaggtatgtc aggtgggact tgggttgatg ttgtcttgga
      721 acatggaggt tgtgtcaccg taatggcaca ggacaaaccg actgtcgaca tagagctggt
      781 tacaacaaca gtcagcaaca tggcggaggt aagatcctac tgctatgagg catcaatatc
      841 agacatggct tcggacagcc gctgcccaac acaaggtgaa gcctaccttg acaagcaatc
      901 agacactcaa tatgtctgca aaagaacgtt agtggacaga ggctggggaa atggatgtgg
      961 actttttggc aaagggagcc tggtgacatg cgctaagttt gcatgctcca agaaaatgac
     1021 cgggaagagc atccagccag agaatctgga gtaccggata atgctgtcag ttcatggctc
     1081 ccagcacagt gggatgatcg ttaatgacac aggacatgaa actgatgaga atagagcgaa
     1141 ggttgagata acgcccaatt caccaagagc cgaagccacc ctggggggtt ttggaagcct
     1201 aggacttgat tgtgaaccga ggacaggcct tgacttttca gatttgtatt acttgactat
     1261 gaataacaag cactggttgg tccacaagga gtggttccac gacattccat taccttggca
     1321 cgctggggca gacaccggaa ctccacactg gaacaacaaa gaagcactgg tagagttcaa
     1381 ggacgcacat gccaaaaggc aaactgtcgt ggttctaggg agtcaagaag gagcagttca
     1441 cacggccctt gctggagctc tggaggctga gatggatggt gcaaagggaa ggctgtcctc
     1501 tggccacttg aaatgtcgcc tgaaaatgga taaacttaga ttgaagggcg tgtcatactc
     1561 cttgtgtacc gcagcgttca cattcaccaa gatcccggct gaaacactgc acgggacagt
     1621 cacagtggag gtacagtacg cagggacaga tggaccttgc aaggttccag ctcagatggc
     1681 ggtggacatg caaactctga ccccagttgg gaggttgata accgctaacc ccgtaatcac
     1741 tgaaagcact gagaactcta agatgatgct ggaacttgat ccaccatttg gggactctta
     1801 cattgtcata ggagtcgggg agaagaagat cacccaccac tggcacagga gtggcagcac
     1861 cattggaaaa gcatttgaag ccactgtgag aggtgccaag agaatggcag tcttgggaga
     1921 cacagcctgg gactttggat cagttggagg cgctctcaac tcattgggca agggcatcca
     1981 tcaaattttt ggagcagctt tcaaatcatt gtttggagga atgtcctggt tctcacaaat
     2041 cctcattgga acgttgctga tgtggttggg tctgaacaca aagaatggat ctatttccct
     2101 tatgtgcttg gccttagggg gagtgttgat cttcttatcc acagccgtct ctgctaaggg
     2161 cgaattcgac ccagctttct tgtacaaagt ggttgatatc cagcacagtg gcggccgctc
     2221 gagtctagag ggcccgcggt tcgaaggtaa gcctatccct aaccctctcc tcggtctcga
     2281 ttctacgcgt accggttagt aatgatcgac aatcaacctc tggattacaa aatttgtgaa
     2341 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     2401 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     2461 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     2521 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     2581 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     2641 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     2701 gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat tctgcgcggg
     2761 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     2821 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
     2881 ctttgggccg cctccccgcc tggcgatggt accggtgtgg aaagtcccca ggctccccag
     2941 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
     3001 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
     3061 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     3121 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc
     3181 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg
     3241 gagcttgtat atccattttc ggatctgatc agcacgtgtt gacaattaat catcggcata
     3301 gtatatcggc atagtataat acgacaaggt gaggaactaa accatggcca agcctttgtc
     3361 tcaagaagaa tccaccctca ttgaaagagc aacggctaca atcaacagca tccccatctc
     3421 tgaagactac agcgtcgcca gcgcagctct ctctagcgac ggccgcatct tcactggtgt
     3481 caatgtatat cattttactg ggggaccttg tgcagaactc gtggtgctgg gcactgctgc
     3541 tgctgcggca gctggcaacc tgacttgtat cgtcgcgatc ggaaatgaga acaggggcat
     3601 cttgagcccc tgcggacggt gccgacaggt gcttctcgat ctgcatcctg ggatcaaagc
     3661 catagtgaag gacagtgatg gacagccgac ggcagttggg attcgtgaat tgctgccctc
     3721 tggttatgtg tgggagggct aagcacaatt cgagctcggt acctttaaga ccaatgactt
     3781 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa
     3841 ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc
     3901 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     3961 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     4021 gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc
     4081 ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt
     4141 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     4201 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     4261 tctggctcta gctatcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     4321 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     4381 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc tagggacgta
     4441 cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt
     4501 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
     4561 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
     4621 ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     4681 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     4741 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     4801 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     4861 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     4921 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     4981 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     5041 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca
     5101 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     5161 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     5221 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     5281 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     5341 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     5401 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     5461 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     5521 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     5581 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     5641 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     5701 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     5761 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     5821 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     5881 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     5941 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     6001 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     6061 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     6121 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     6181 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     6241 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     6301 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     6361 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     6421 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     6481 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     6541 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     6601 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     6661 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     6721 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     6781 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     6841 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     6901 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     6961 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     7021 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     7081 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
     7141 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
     7201 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc
     7261 gcgcaattaa ccctcactaa agggaacaaa agctggagct gcaagcttaa tgtagtctta
     7321 tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg ccttacaagg
     7381 agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc gtgccttatt
     7441 aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc cgcattgcag
     7501 agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt tagaccagat
     7561 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     7621 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     7681 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg
     7741 aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc
     7801 acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc
     7861 tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga
     7921 tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta
     7981 tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa
     8041 ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt
     8101 agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa
     8161 gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca
     8221 cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     8281 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     8341 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     8401 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     8461 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     8521 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     8581 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     8641 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     8701 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     8761 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     8821 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     8881 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     8941 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     9001 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     9061 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcggttaa cttttaaaag
     9121 aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga
     9181 catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaatttta tcgataagct
     9241 tgggagttcc
//
