LOCUS       sequence-241009-       11606 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of BMPR2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11606)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 11606)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-BMPR2
FEATURES             Location/Qualifiers
     source          1..11606
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2223..3401
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2454..3392
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3349..3369
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    3421..3445
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6566..6590)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        6598..6616
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6641..6660)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6642..6660)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(6666..6686)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             6740..6781
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    6802..7369
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    6819..7371
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6963..6980)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7190..7209
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7383..7982
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7383..7402)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7879..7899
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7996..8584
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8049..8069)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8467..8478)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8659..8892
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(9011..9029)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9097..9201
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9202..9270,9271..10062)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9420..9439)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10233..10821
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10722..10741
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10975..10992
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11232..11253
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11268..11273,11274..11291,11292..11298)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11306..11322
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11311..11333
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11330..11346
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11330..11346
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11396..11415
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccct gcagatatca
     3421 acaagtttgt acaaaaaagc aggcaccatg acttcctcgc tgcagcggcc ctggcgggtg
     3481 ccctggctac catggaccat cctgctggtc agcactgcgg ctgcttcgca gaatcaagaa
     3541 cggctatgtg cgtttaaaga tccgtatcag caagaccttg ggataggtga gagtagaatc
     3601 tctcatgaaa atgggacaat attatgctcg aaaggtagca cctgctatgg cctttgggag
     3661 aaatcaaaag gggacataaa tcttgtaaaa caaggatgtt ggtctcacat tggagatccc
     3721 caagagtgtc actatgaaga atgtgtagta actaccactc ctccctcaat tcagaatgga
     3781 acataccgtt tctgctgttg tagcacagat ttatgtaatg tcaactttac tgagaatttt
     3841 ccacctcctg acacaacacc actcagtcca cctcattcat ttaaccgaga tgagacaata
     3901 atcattgctt tggcatcagt ctctgtatta gctgttttga tagttgcctt atgctttgga
     3961 tacagaatgt tgacaggaga ccgtaaacaa ggtcttcaca gtatgaacat gatggaggca
     4021 gcagcatccg aaccctctct tgatctagat aatctgaaac tgttggagct gattggccga
     4081 ggtcgatatg gagcagtata taaaggctcc ttggatgagc gtccagttgc tgtaaaagtg
     4141 ttttcctttg caaaccgtca gaattttatc aacgaaaaga acatttacag agtgcctttg
     4201 atggaacatg acaacattgc ccgctttata gttggagatg agagagtcac tgcagatgga
     4261 cgcatggaat atttgcttgt gatggagtac tatcccaatg gatctttatg caagtattta
     4321 agtctccaca caagtgactg ggtaagctct tgccgtcttg ctcattctgt tactagagga
     4381 ctggcttatc ttcacacaga attaccacga ggagatcatt ataaacctgc aatttcccat
     4441 cgagatttaa acagcagaaa tgtcctagtg aaaaatgatg gaacctgtgt tattagtgac
     4501 tttggactgt ccatgaggct gactggaaat agactggtgc gcccagggga ggaagataat
     4561 gcagccataa gcgaggttgg cactatcaga tatatggcac cagaagtgct agaaggagct
     4621 gtgaacttga gggactgtga atcagctttg aaacaagtag acatgtatgc tcttggacta
     4681 atctattggg agatatttat gagatgtaca gacctcttcc caggggaatc cgtaccagag
     4741 taccagatgg cttttcagac agaggttgga aaccatccca cttttgagga tatgcaggtt
     4801 ctcgtgtcta gggaaaaaca gagacccaag ttcccagaag cctggaaaga aaatagcctg
     4861 gcagtgaggt cactcaagga gacaatcgaa gactgttggg accaggatgc agaggctcgg
     4921 cttactgcac agtgtgctga ggaaaggatg gctgaactta tgatgatttg ggaaagaaac
     4981 aaatctgtga gcccaacagt caatccaatg tctactgcta tgcagaatga acgcaacctg
     5041 tcacataata ggcgtgtgcc aaaaattggt ccttatccag attattcttc ctcctcatac
     5101 attgaagact ctatccatca tactgacagc atcgtgaaga atatttcctc tgagcattct
     5161 atgtccagca cacctttgac tataggggaa aaaaaccgaa attcaattaa ctatgaacga
     5221 cagcaagcac aagctcgaat ccccagccct gaaacaagtg tcaccagcct ctccaccaac
     5281 acaacaacca caaacaccac aggactcacg ccaagtactg gcatgactac tatatctgag
     5341 atgccatacc cagatgaaac aaatctgcat accacaaatg ttgcacagtc aattgggcca
     5401 acccctgtct gcttacagct gacagaagaa gacttggaaa ccaacaagct agacccaaaa
     5461 gaagttgata agaacctcaa ggaaagctct gatgagaatc tcatggagca ctctcttaaa
     5521 cagttcagtg gcccagaccc actgagcagt actagttcta gcttgcttta cccactcata
     5581 aaacttgcag tagaagcaac tggacagcag gacttcacac agactgcaaa tggccaagca
     5641 tgtttgattc ctgatgttct gcctactcag atctatcctc tccccaagca gcagaacctt
     5701 cccaagagac ctactagttt gcctttgaac accaaaaatt caacaaaaga gccccggcta
     5761 aaatttggca gcaagcacaa atcaaacttg aaacaagtcg aaactggagt tgccaagatg
     5821 aatacaatca atgcagcaga acctcatgtg gtgacagtca ccatgaatgg tgtggcaggt
     5881 agaaaccaca gtgttaactc ccatgctgcc acaacccaat atgccaatgg gacagtacta
     5941 tctggccaaa caaccaacat agtgacacat agggcccaag aaatgttgca gaatcagttt
     6001 attggtgagg acacccggct gaatattaat tccagtcctg atgagcatga gcctttactg
     6061 agacgagagc aacaagctgg ccatgatgaa ggtgttctgg atcgtcttgt ggacaggagg
     6121 gaacggccac tagaaggtgg ccgaactaat tccaataaca acaacagcaa tccatgttca
     6181 gaacaagatg ttcttgcaca gggtgttcca agcacagcag cagatcctgg gccatcaaag
     6241 cccagaagag cacagaggcc taattctctg gatctttcag ccacaaatgt cctggatggc
     6301 agcagtatac agataggtga gtcaacacaa gatggcaaat caggatcagg tgaaaagatc
     6361 aagaaacgtg tgaaaactcc ctattctctt aagcggtggc gcccctccac ctgggtcatc
     6421 tccactgaat cgctggactg tgaagtcaac aataatggca gtaacagggc agttcattcc
     6481 aaatccagca ctgctgttta ccttgcagaa ggaggcactg ctacaaccat ggtgtctaaa
     6541 gatataggaa tgaactgtct gtagaaccca gctttcttgt acaaagtggt gatatccaat
     6601 taaccctcac taaagggatt agtatttagt agtaatagag ccctatagtg agtcgtatta
     6661 ccaccccact tttctataca aagtggttga tatccagcac agtggcggcc gctcgagtct
     6721 agagggcccg cggttcgaag gtaagcctat ccctaaccct ctcctcggtc tcgattctac
     6781 gcgtaccggt tagtaatgag atccctcccc cccccctaac gttactggcc gaagccgctt
     6841 ggaataaggc cggtgtgcgt ttgtctatat gttattttcc accatattgc cgtcttttgg
     6901 caatgtgagg gcccggaaac ctggccctgt cttcttgacg agcattccta ggggtctttc
     6961 ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga
     7021 agcttcttga agacaaacaa cgtctgtagc gaccctttgc aggcagcgga accccccacc
     7081 tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa gatacacctg caaaggcggc
     7141 acaaccccag tgccacgttg tgagttggat agttgtggaa agagtcaaat ggctctcctc
     7201 aagcgtattc aacaaggggc tgaaggatgc ccagaaggta ccccattgta tgggatctga
     7261 tctggggcct cggtgcacat gctttacatg tgtttagtcg aggttaaaaa aacgtctagg
     7321 ccccccgaac cacggggacg tggttttcct ttgaaaaaca cgatgataat atggccacac
     7381 atatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg
     7441 tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg
     7501 accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg
     7561 acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg
     7621 agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg
     7681 gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg
     7741 agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg
     7801 gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc
     7861 tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg
     7921 ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct
     7981 aaatcgatag atcctaatca acctctggat tacaaaattt gtgaaagatt gactggtatt
     8041 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     8101 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
     8161 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
     8221 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
     8281 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     8341 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc
     8401 tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
     8461 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
     8521 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
     8581 ccgcctgaga tcctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt
     8641 taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt
     8701 ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     8761 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     8821 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     8881 aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga
     8941 aatgaatatc agagagtgag aggcccgggt taattaagga aagggctaga tcattcttga
     9001 agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
     9061 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     9121 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     9181 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     9241 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     9301 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     9361 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     9421 ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata
     9481 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     9541 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     9601 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     9661 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     9721 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     9781 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     9841 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     9901 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     9961 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
    10021 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
    10081 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
    10141 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
    10201 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
    10261 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
    10321 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
    10381 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
    10441 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
    10501 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
    10561 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
    10621 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
    10681 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
    10741 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
    10801 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
    10861 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
    10921 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
    10981 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
    11041 ccgattcatt aatgcagcaa gctcatggct gactaatttt ttttatttat gcagaggccg
    11101 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
    11161 gcttttgcaa aaagctcccc gtggcacgac aggtttcccg actggaaagc gggcagtgag
    11221 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg
    11281 cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc
    11341 tatgacatga ttacgaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
    11401 ttgtccaaac tcatcaatgt atcttatcat gtctggatca actggataac tcaagctaac
    11461 caaaatcatc ccaaacttcc caccccatac cctattacca ctgccaatta cctgtggttt
    11521 catttactct aaacctgtga ttcctctgaa ttattttcat tttaaagaaa ttgtatttgt
    11581 taaatatgta ctacaaactt agtagt
//
