LOCUS       sequence-241014-        9589 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of ZIKV protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9589)
  TITLE     Rehwinkel Lab plasmids
REFERENCE   2  (bases 1 to 9589)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLenti6.3/NS3
FEATURES             Location/Qualifiers
     source          1..9589
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    510..528
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    531..549
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    590..614
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2508..2532)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2585..2626
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2650..3238
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2703..2723)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3121..3132)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        3253..3569
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3420..3555
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        3617..3664
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             3683..4081
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             4168..4401
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4473..4594
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4510..4529)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4564..4583
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4634..4769
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4789..4808)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4790..4808)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4818..4835)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4818..4834)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4827..4849)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4976..5431
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5063..5082)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5273..5294
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5457..5561
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5562..5630,5631..6422)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5780..5799)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6593..7181
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7082..7101
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7335..7352
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7469..7490
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7505..7510,7511..7528,7529..7535)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7543..7559
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7548..7570
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7567..7583
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7567..7583
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7602..7622
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7604..7622
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7650..7876
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7877..8057
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8104..8229
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8722..8955
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9140..9184
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9451..9568
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 gcttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc tccctatcag
      541 tgatagagat cgtcgactag tccagtgtgg tggaattctg cagatatcaa caagtttgta
      601 caaaaaagca ggctccgaat tcgcccttag gaggacagct atgagtggtg ctctatggga
      661 tgtgcctgct cccaaggaag taaaaaaggg ggagaccaca gatggagtgt acagagtaat
      721 gactcgtaga ctgctaggtt caacacaagt tggagtggga gttatgcaag agggggtctt
      781 tcacactatg tggcacgtca caaaaggatc cgcgctgaga agcggtgaag ggagacttga
      841 tccatactgg ggagatgtca agcaggatct ggtgtcatac tgtggtccat ggaagctaga
      901 tgccgcctgg gacgggcaca gcgaggtgca gctcttggcc gtgccccccg gagagagagc
      961 gaggaacatc cagactctgc ccggaatatt taagacaaag gatggggaca ttggagcggt
     1021 tgcgctggat tacccagcag gaacttcagg atctccaatc ctagacaagt gtgggagagt
     1081 gataggactt tatggcaatg gggtcgtgat caaaaatggg agttatgtta gtgccatcac
     1141 ccaagggagg agggaggaag agactcctgt tgagtgcttc gagccttcga tgctgaagaa
     1201 gaagcagcta actgtcttag acttgcatcc tggagctggg aaaaccagga gagttcttcc
     1261 tgaaatagtc cgtgaagcca taaaaacaag actccgtact gtgatcttag ctccaaccag
     1321 ggttgtcgct gctgaaatgg aggaagccct tagagggctt ccagtgcgtt atatgacaac
     1381 agcagtcaat gtcacccact ctggaacaga aatcgtcgac ttaatgtgcc atgccacctt
     1441 cacttcacgt ctactacagc caatcagagt ccccaactat aatctgtata ttatggatga
     1501 ggcccacttc acagatccct caagtatagc agcaagagga tacatttcaa caagggttga
     1561 gatgggcgag gcggctgcca tcttcatgac cgccacgcca ccaggaaccc gtgacgcatt
     1621 tccggactcc aactcaccaa ttatggacac cgaagtggaa gtcccagaga gagcctggag
     1681 ctcaggcttt gattgggtga cggatcattc tggaaaaaca gtttggtttg ttccaagcgt
     1741 gaggaacggc aatgagatcg cagcttgtct gacaaaggct ggaaaacggg tcatacagct
     1801 cagcagaaag acttttgaga cagagttcca gaaaacaaaa catcaagagt gggactttgt
     1861 cgtgacaact gacatttcag agatgggcgc caactttaaa gctgaccgtg tcatagattc
     1921 caggagatgc ctaaagccgg tcatacttga tggcgagaga gtcattttgg ctggacccat
     1981 gcctgtcaca catgccagcg ctgcccagag gagggggcgc ataggcagga atcccaacaa
     2041 acctggagat gagtatctgt atggaggtgg gtgcgcagag actgacgaag accatgcaca
     2101 ctggcttgaa gcaagaatgc tccttgacaa tatttacctc caagatggcc tcatagcctc
     2161 gctctatcga cctgaggccg acaaagtagc agccattgag ggagagttca agcttaggac
     2221 ggagcaaagg aagacctttg tggaactcat gaaaagagga gatcttcctg tttggctggc
     2281 ctatcaggtt gcatctgccg gaataaccta cacagataga agatggtgct ttgatggcac
     2341 gaccaacaac accataatgg aagacagtgt gccggcagag gtgtggacca gacacggaga
     2401 gaaaagagtg ctcaaaccga ggtggatgga cgccagagtt tgttcagatc atgcggccct
     2461 gaagtcattc aaggagtttg ccgctgggaa aagaaagggc gaattcgacc cagctttctt
     2521 gtacaaagtg gttgatatcc agcacagtgg cggccgctcg agtctagagg gcccgcggtt
     2581 cgaaggtaag cctatcccta accctctcct cggtctcgat tctacgcgta ccggttagta
     2641 atgatcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     2701 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     2761 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     2821 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     2881 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     2941 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     3001 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca
     3061 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     3121 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
     3181 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct
     3241 ggcgatggta ccggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     3301 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
     3361 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
     3421 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     3481 ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag
     3541 gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg
     3601 gatctgatca gcacgtgttg acaattaatc atcggcatag tatatcggca tagtataata
     3661 cgacaaggtg aggaactaaa ccatggccaa gcctttgtct caagaagaat ccaccctcat
     3721 tgaaagagca acggctacaa tcaacagcat ccccatctct gaagactaca gcgtcgccag
     3781 cgcagctctc tctagcgacg gccgcatctt cactggtgtc aatgtatatc attttactgg
     3841 gggaccttgt gcagaactcg tggtgctggg cactgctgct gctgcggcag ctggcaacct
     3901 gacttgtatc gtcgcgatcg gaaatgagaa caggggcatc ttgagcccct gcggacggtg
     3961 ccgacaggtg cttctcgatc tgcatcctgg gatcaaagcc atagtgaagg acagtgatgg
     4021 acagccgacg gcagttggga ttcgtgaatt gctgccctct ggttatgtgt gggagggcta
     4081 agcacaattc gagctcggta cctttaagac caatgactta caaggcagct gtagatctta
     4141 gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag
     4201 atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct
     4261 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     4321 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     4381 cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag tatttataac
     4441 ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt
     4501 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     4561 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc
     4621 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     4681 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
     4741 gaagtagtga ggaggctttt ttggaggcct agggacgtac ccaattcgcc ctatagtgag
     4801 tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
     4861 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
     4921 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg
     4981 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     5041 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     5101 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     5161 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     5221 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     5281 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     5341 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     5401 aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg
     5461 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     5521 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     5581 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     5641 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     5701 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     5761 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     5821 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     5881 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     5941 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     6001 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     6061 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     6121 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     6181 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     6241 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     6301 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     6361 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     6421 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     6481 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     6541 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     6601 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     6661 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     6721 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     6781 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     6841 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     6901 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     6961 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     7021 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     7081 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     7141 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     7201 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     7261 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     7321 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
     7381 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
     7441 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
     7501 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
     7561 ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa
     7621 gggaacaaaa gctggagctg caagcttaat gtagtcttat gcaatactct tgtagtcttg
     7681 caacatggta acgatgagtt agcaacatgc cttacaagga gagaaaaagc accgtgcatg
     7741 ccgattggtg gaagtaaggt ggtacgatcg tgccttatta ggaaggcaac agacgggtct
     7801 gacatggatt ggacgaacca ctgaattgcc gcattgcaga gatattgtat ttaagtgcct
     7861 agctcgatac ataaacgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     7921 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     7981 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     8041 gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag
     8101 gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg
     8161 cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc
     8221 gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg
     8281 ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa
     8341 cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga
     8401 cagctacaac catcccttca gacaggatca gaagaactta gatcattata taatacagta
     8461 gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac
     8521 aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc cgctgatctt
     8581 cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata aatataaagt
     8641 agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag
     8701 agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag
     8761 cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat
     8821 agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact
     8881 cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa
     8941 ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt
     9001 gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc acacgacctg
     9061 gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct taattgaaga
     9121 atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata aatgggcaag
     9181 tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat tcataatgat
     9241 agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag tgaatagagt
     9301 taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga ggggacccga
     9361 caggcccgaa ggaatagaag aagaaggtgg agagagagac agagacagat ccattcgatt
     9421 agtgaacgga tctcgacggt atcggttaac ttttaaaaga aaagggggga ttggggggta
     9481 cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca
     9541 aaaacaaatt acaaaaattc aaaattttat cgataagctt gggagttcc
//
