LOCUS       sequence-241017-       12958 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of CDK12.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12958)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 12958)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-CDK12
FEATURES             Location/Qualifiers
     source          1..12958
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2223..3401
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2454..3392
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3349..3369
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    3421..3445
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(7895..7919)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             7972..8013
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    8034..8601
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    8051..8603
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(8195..8212)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     8422..8441
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(8615..8617,8618..8620,8621..9334)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(8615..8617,8618..8620,8621..9331)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8660..8681)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8921..8940)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     9268..9289
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    9348..9936
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9401..9421)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9819..9830)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             10011..10244
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(10363..10381)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10449..10553
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10554..10622,10623..11414)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10772..10791)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11585..12173
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12074..12093
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12327..12344
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12584..12605
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12620..12625,12626..12643,12644..12650)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12658..12674
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12663..12685
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12682..12698
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12682..12698
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12748..12767
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccct gcagatatca
     3421 acaagtttgt acaaaaaagc aggcaccatg cccaattcag agagacatgg gggcaagaag
     3481 gacgggagtg gaggagcttc tggaactttg cagccgtcat cgggaggcgg cagctctaac
     3541 agcagagagc gtcaccgctt ggtatcgaag cacaagcggc ataagtccaa acactccaaa
     3601 gacatggggt tggtgacccc cgaagcagca tccctgggca cagttatcaa acctttggtg
     3661 gagtatgatg atatcagctc tgattccgac accttctccg atgacatggc cttcaaacta
     3721 gaccaaaggg agaacgacga acgtcgtgga tcagatcgga gcgaccgcct gcacaaacat
     3781 cgtcaccacc agcacaggcg ttcccgggac ttactaaaag ctaaacagac cgaaaaagaa
     3841 aaaagccaag aagtctccag caagtcggga tcgatgaagg accggatatc gggaagttca
     3901 aagcgttcga atgaggagac tgatgactat gggaaggcgc aggtagccaa aagcagcagc
     3961 aaggaatcca ggtcatccaa gctccacaag gagaagacca ggaaagaacg ggagctgaag
     4021 tctgggcaca aagaccggag taaaagtcat cgaaaaaggg aaacacccaa aagttacaaa
     4081 acagtggaca gcccaaaacg gagatccagg agcccccaca ggaagtggtc tgacagctcc
     4141 aaacaagatg atagcccctc gggagcttct tatggccaag attatgacct tagtccctca
     4201 cgatctcata cctcgagcaa ttatgactcc tacaagaaaa gtcctggaag tacctcgaga
     4261 aggcagtcgg tcagtccccc ttacaaggag ccttcggcct accagtccag cacccggtca
     4321 ccgagcccct acagtaggcg acagagatct gtcagtccct atagcaggag acggtcgtcc
     4381 agctacgaaa gaagtggctc ttacagcggg cgatcgccca gtccctatgg tcgaaggcgg
     4441 tccagcagcc ctttcctgag caagcggtct ctgagtcgga gtccactccc cagtaggaaa
     4501 tccatgaagt ccagaagtag aagtcctgca tattcaagac attcatcttc tcatagtaaa
     4561 aagaagagat ccagttcacg cagtcgtcat tccagtatct cacctgtcag gcttccactt
     4621 aattccagtc tgggagctga actcagtagg aaaaagaagg aaagagcagc tgctgctgct
     4681 gcagcaaaga tggatggaaa ggagtccaag ggttcacctg tatttttgcc tagaaaagag
     4741 aacagttcag tagaggctaa ggattcaggt ttggagtcta aaaagttacc cagaagtgta
     4801 aaattggaaa aatctgcccc agatactgaa ctggtgaatg taacacatct aaacacagag
     4861 gtaaaaaatt cttcagatac agggaaagta aagttggatg agaactccga gaagcatctt
     4921 gttaaagatt tgaaagcaca gggaacaaga gactctaaac ccatagcact gaaagaggag
     4981 attgttactc caaaggagac agaaacatca gaaaaggaga cccctccacc tcttcccaca
     5041 attgcttctc ccccaccccc tctaccaact actacccctc cacctcagac accccctttg
     5101 ccacctttgc ctccaatacc agctcttcca cagcaaccac ctctgcctcc ttctcagcca
     5161 gcatttagtc aggttcctgc ttccagtact tcaactttgc ccccttctac tcactcaaag
     5221 acatctgctg tgtcctctca ggcaaattct cagccccctg tacaggtttc tgtgaagact
     5281 caagtatctg taacagctgc tattccacac ctgaaaactt caacgttgcc tcctttgccc
     5341 ctcccaccct tattacctgg agatgatgac atggatagtc caaaagaaac tcttccttca
     5401 aaacctgtga agaaagagaa ggaacagagg acacgtcact tactcacaga ccttcctctc
     5461 cctccagagc tccctggtgg agatctgtct cccccagact ctccagaacc aaaggcaatc
     5521 acaccacctc agcaaccata taaaaagaga ccaaaaattt gttgtcctcg ttatggagaa
     5581 agaagacaaa cagaaagcga ctgggggaaa cgctgtgtgg acaagtttga cattattggg
     5641 attattggag aaggaaccta tggccaagta tataaagcca aggacaaaga cacaggagaa
     5701 ctagtggctc tgaagaaggt gagactagac aatgagaaag agggcttccc aatcacagcc
     5761 attcgtgaaa tcaaaatcct tcgtcagtta atccaccgaa gtgttgttaa catgaaggaa
     5821 attgtcacag ataaacaaga tgcactggat ttcaagaagg acaaaggtgc cttttacctt
     5881 gtatttgagt atatggacca tgacttaatg ggactgctag aatctggttt ggtgcacttt
     5941 tctgaggacc atatcaagtc gttcatgaaa cagctaatgg aaggattgga atactgtcac
     6001 aaaaagaatt tcctgcatcg ggatattaag tgttctaaca ttttgctgaa taacagtggg
     6061 caaatcaaac tagcagattt tggacttgct cggctctata actctgaaga gagtcgccct
     6121 tacacaaaca aagtcattac tttgtggtac cgacctccag aactactgct aggagaggaa
     6181 cgttacacac cagccataga tgtttggagc tgtggatgta ttcttgggga actattcaca
     6241 aagaagccta tttttcaagc caatctggaa ctggctcagc tagaactgat cagccgactt
     6301 tgtggtagcc cttgtccagc tgtgtggcct gatgttatca aactgcccta cttcaacacc
     6361 atgaaaccga agaagcaata tcgaaggcgt ctacgagaag aattctcttt cattccttct
     6421 gcagcacttg atttattgga ccacatgctg acactagatc ctagtaagcg gtgcacagct
     6481 gaacagaccc tacagagcga cttccttaaa gatgtcgaac tcagcaaaat ggctcctcca
     6541 gacctccccc actggcagga ttgccatgag ttgtggagta agaaacggcg acgtcagcga
     6601 caaagtggtg ttgtagtcga agagccacct ccatccaaaa cttctcgaaa agaaactacc
     6661 tcagggacaa gtactgagcc tgtgaagaac agcagcccag caccacctca gcctgctcct
     6721 ggcaaggtgg agtctggggc tggggatgca ataggccttg ctgacatcac acaacagctg
     6781 aatcaaagtg aattggcagt gttattaaac ctgctgcaga gccaaaccga cctgagcatc
     6841 cctcaaatgg cacagctgct taacatccac tccaacccag agatgcagca gcagctggaa
     6901 gccctgaacc aatccatcag tgccctgacg gaagctactt cccagcagca ggactcagag
     6961 accatggccc cagaggagtc tttgaaggaa gcaccctctg ccccagtgat cctgccttca
     7021 gcagaacaga cgacccttga agcttcaagc acaccagctg acatgcagaa tatattggca
     7081 gttctcttga gtcagctgat gaaaacccaa gagccagcag gcagtctgga ggaaaacaac
     7141 agtgacaaga acagtgggcc acaggggccc cgaagaactc ccacaatgcc acaggaggag
     7201 gcagcagaga agaggccccc tgagcccccc ggacctccac cgccgccacc tccaccccct
     7261 ctggttgaag gcgatctttc cagcgccccc caggagttga acccagccgt gacagccgcc
     7321 ttgctgcaac ttttatccca gcctgaagca gagcctcctg gccacctgcc acatgagcac
     7381 caggccttga gaccaatgga gtactccacc cgaccccgtc caaacaggac ttatggaaac
     7441 actgatgggc ctgaaacagg gttcagtgcc attgacactg atgaacgaaa ctctggtcca
     7501 gccttgacag aatccttggt ccagaccctg gtgaagaaca ggaccttctc aggctctctg
     7561 agccaccttg gggagtccag cagttaccag ggcacagggt cagtgcagtt tccaggggac
     7621 caggacctcc gttttgccag ggtcccctta gcgttacacc cggtggtcgg gcaaccattc
     7681 ctgaaggctg agggaagcag caattctgtg gtacatgcag agaccaaatt gcaaaactat
     7741 ggggagctgg ggccaggaac cactggggcc agcagctcag gagcaggcct tcactggggg
     7801 ggcccaactc agtcttctgc ttatggaaaa ctctatcggg ggcctacaag agtcccacca
     7861 agagggggaa gagggagagg agttccttac tagaacccag ctttcttgta caaagtggtt
     7921 gatatccagc acagtggcgg ccgctcgagt ctagagggcc cgcggttcga aggtaagcct
     7981 atccctaacc ctctcctcgg tctcgattct acgcgtaccg gttagtaatg agatccctcc
     8041 ccccccccta acgttactgg ccgaagccgc ttggaataag gccggtgtgc gtttgtctat
     8101 atgttatttt ccaccatatt gccgtctttt ggcaatgtga gggcccggaa acctggccct
     8161 gtcttcttga cgagcattcc taggggtctt tcccctctcg ccaaaggaat gcaaggtctg
     8221 ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt gaagacaaac aacgtctgta
     8281 gcgacccttt gcaggcagcg gaacccccca cctggcgaca ggtgcctctg cggccaaaag
     8341 ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg
     8401 atagttgtgg aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg gctgaaggat
     8461 gcccagaagg taccccattg tatgggatct gatctggggc ctcggtgcac atgctttaca
     8521 tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga accacgggga cgtggttttc
     8581 ctttgaaaaa cacgatgata atatggccac acatatggtg agcaagggcg aggagctgtt
     8641 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
     8701 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg
     8761 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt
     8821 gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
     8881 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac
     8941 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
     9001 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca
     9061 caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg
     9121 ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat
     9181 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag
     9241 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg
     9301 gatcactctc ggcatggacg agctgtacaa gtaaatcgat agatcctaat caacctctgg
     9361 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
     9421 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
     9481 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca
     9541 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg
     9601 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg
     9661 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
     9721 attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca
     9781 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc
     9841 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc
     9901 agacgagtcg gatctccctt tgggccgcct ccccgcctga gatcctttaa gaccaatgac
     9961 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct
    10021 aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga
    10081 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
    10141 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
    10201 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca
    10261 tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggcccgg
    10321 gttaattaag gaaagggcta gatcattctt gaagacgaaa gggcctcgtg atacgcctat
    10381 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
    10441 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
    10501 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
    10561 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
    10621 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
    10681 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
    10741 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg
    10801 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
    10861 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
    10921 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
    10981 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
    11041 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
    11101 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
    11161 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
    11221 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
    11281 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
    11341 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
    11401 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
    11461 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
    11521 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
    11581 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
    11641 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
    11701 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
    11761 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
    11821 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
    11881 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
    11941 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
    12001 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
    12061 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
    12121 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
    12181 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
    12241 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
    12301 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
    12361 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc aagctcatgg
    12421 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
    12481 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc ccgtggcacg
    12541 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
    12601 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
    12661 tgagcggata acaatttcac acaggaaaca gctatgacat gattacgaat ttcacaaata
    12721 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
    12781 atgtctggat caactggata actcaagcta accaaaatca tcccaaactt cccaccccat
    12841 accctattac cactgccaat tacctgtggt ttcatttact ctaaacctgt gattcctctg
    12901 aattattttc attttaaaga aattgtattt gttaaatatg tactacaaac ttagtagt
//
