LOCUS       sequence-241023-        2804 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GoldenBraid2.0 Universal Part Domesticator #3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2804)
  AUTHORS   Sarrion-Perdigones A, Chang L, Gonzalez Y, Gallego-Flores T, Young 
            DW, Venken KJT
  TITLE     Examining multiple cellular pathways at once using multiplex 
            hextuple luciferase assaying.
  JOURNAL   Nat Commun. 2019 Dec 13;10(1):5710. doi: 10.1038/s41467-019-13651-y.
  PUBMED    31836712
REFERENCE   2  (bases 1 to 2804)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-12-13"; volume: "10(1):5710. doi"; pages: " 
            10.1038/s41467-019-13651-y"
COMMENT     Sequence Label: pUPD3
FEATURES             Location/Qualifiers
     source          1..2804
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     14..31
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     15..31
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     38..57
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        38..56
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        110..157
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     primer_bind     complement(532..551)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        complement(601..619)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(602..619)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(635..651)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(635..651)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      complement(766..1354)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(846..865)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        1469..1571
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             1572..2231
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1638..1657)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      2344..2799
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2431..2450)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2641..2662
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ttgtagaaac gcatgtaaaa cgacggccag tgaattgtaa tacgactcac tatagggtga
       61 attccgctta ggaccttaga tattgatctc aaggtctcgt gagacgcctg ttgacaatta
      121 atcatcggca tagtatatcg gcatagtata atacgacaag gtgaggaact aaacccagga
      181 ggcagagatc atgactatga ttacgccaag ctatctgggc gatactatcg aatactctag
      241 cttgcatgcg tgcaggtcga ctctagaaga tccccgggta ccgagctcta attcgccgta
      301 cagcgagtct tactacaatt ctctggcggt ggtcttgcaa cgtcgtgact gggaaaaccc
      361 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag
      421 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatgacg
      481 cctgatgcgg tattttctcc ttacgttact gtgcggtatt tccactctca gtacaatctg
      541 ctctgatgcc gcatgtgcgt ctcacgagac cactcatcgc ccaatcacta gtgaattcgt
      601 tctatagtgt cacctaaata ccatagagcc gttggtcata gctgtttcct gtccaggcat
      661 catgacacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
      721 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
      781 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
      841 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
      901 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
      961 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     1021 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     1081 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     1141 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     1201 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     1261 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     1321 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     1381 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     1441 aaggatcttc acctagatcc ttttgtgatg atcggcacgt aagaggttcc aactttcacc
     1501 ataatgaaat aagatcacta ccgggcgtat tttttgagtt atcgagattt tcaggagcta
     1561 aggaagctaa aatggagaaa aaaatcactg gatataccac cgttgatata tcccaatggc
     1621 atcgtaaaga acattttgag gcatttcagt cagttgctca atgtacctat aaccagaccg
     1681 ttcagctgga tattacggcc tttttaaaga ccgtaaagaa aaataagcac aagttttatc
     1741 cggcctttat tcacattctt gcccgcctga tgaatgctca tccggaattc cgtatggcaa
     1801 tgaaagacgg tgagctggtg atatgggata gtgttcaccc ttgttacacc gttttccatg
     1861 agcaaactga aacgttttca tcgctctgga gtgaatacca cgacgatttc cggcagtttc
     1921 tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag
     1981 ggtttattga gaatatgttt ttcgtgtcag ccaatccctg ggtgagtttc accagttttg
     2041 atttaaacgt ggccaatatg gacaacttct tcgcccccgt tttcaccatg ggcaaatatt
     2101 atacgcaagg cgacaaggtg ctgatgccgc tggcgattca ggttcatcat gccgtttgtg
     2161 atggcttcca tgtcggcaga atgcttaatg aattacaaca gtactgcgat gagtggcagg
     2221 gcggggcgta atttttttaa ggcagttatt ggtgccctta aacgcctggt tgctacgcct
     2281 gaataagtga taataagcgg atgaatggca gaaattcgat gataagctgt caaacatgat
     2341 gttacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     2401 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     2461 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     2521 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     2581 ggccagcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     2641 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     2701 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     2761 ttaacgcgaa ttttaacaaa atattaacgc ttacaatttt aaca
//
