LOCUS       sequence-241025-        2385 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GBPart - Operator/DNA Response element - 5 copies of the E-box 
            motif.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2385)
  AUTHORS   Sarrion-Perdigones A, Chang L, Gonzalez Y, Gallego-Flores T, Young 
            DW, Venken KJT
  TITLE     Examining multiple cellular pathways at once using multiplex 
            hextuple luciferase assaying.
  JOURNAL   Nat Commun. 2019 Dec 13;10(1):5710. doi: 10.1038/s41467-019-13651-y.
  PUBMED    31836712
REFERENCE   2  (bases 1 to 2385)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-12-13"; volume: "10(1):5710. doi"; pages: " 
            10.1038/s41467-019-13651-y"
COMMENT     Sequence Label: p5xE-Box
FEATURES             Location/Qualifiers
     source          1..2385
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     14..31
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     15..31
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     38..57
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        38..56
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(182..200)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(183..200)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(216..232)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(216..232)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      complement(347..935)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(427..446)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        1050..1152
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             1153..1812
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1219..1238)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1925..2380
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2012..2031)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2222..2243
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ttgtagaaac gcatgtaaaa cgacggccag tgaattgtaa tacgactcac tatagggtga
       61 attccgctta ggaccttaga tattgatctc aaggtctcgg ggacacgtga gcacgtgagc
      121 acgtgtccac gtgcacacgt gctcccgaga ccactcatcg cccaatcact agtgaattcg
      181 ttctatagtg tcacctaaat accatagagc cgttggtcat agctgtttcc tgtccaggca
      241 tcatgacacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
      301 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
      361 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
      421 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
      481 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
      541 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
      601 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
      661 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
      721 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
      781 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
      841 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
      901 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
      961 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     1021 aaaggatctt cacctagatc cttttgtgat gatcggcacg taagaggttc caactttcac
     1081 cataatgaaa taagatcact accgggcgta ttttttgagt tatcgagatt ttcaggagct
     1141 aaggaagcta aaatggagaa aaaaatcact ggatatacca ccgttgatat atcccaatgg
     1201 catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta taaccagacc
     1261 gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca caagttttat
     1321 ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt ccgtatggca
     1381 atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac cgttttccat
     1441 gagcaaactg aaacgttttc atcgctctgg agtgaatacc acgacgattt ccggcagttt
     1501 ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa acctggccta tttccctaaa
     1561 gggtttattg agaatatgtt tttcgtgtca gccaatccct gggtgagttt caccagtttt
     1621 gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcaccat gggcaaatat
     1681 tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca tgccgtttgt
     1741 gatggcttcc atgtcggcag aatgcttaat gaattacaac agtactgcga tgagtggcag
     1801 ggcggggcgt aattttttta aggcagttat tggtgccctt aaacgcctgg ttgctacgcc
     1861 tgaataagtg ataataagcg gatgaatggc agaaattcga tgataagctg tcaaacatga
     1921 tgttacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     1981 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     2041 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     2101 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     2161 gggccagcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     2221 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     2281 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     2341 tttaacgcga attttaacaa aatattaacg cttacaattt taaca
//
