LOCUS       sequence-241028-        9156 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express mutant RASA2 in mamalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9156)
  AUTHORS   Arafeh R, Qutob N, Emmanuel R, Keren-Paz A, Madore J, Elkahloun A, 
            Wilmott JS, Gartner JJ, Di Pizio A, Winograd-Katz S, Sindiri S, 
            Rotkopf R, Dutton-Regester K, Johansson P, Pritchard AL, Waddell N, 
            Hill VK, Lin JC, Hevroni Y, Rosenberg SA, Khan J, Ben-Dor S, Niv MY,
            Ulitsky I, Mann GJ, Scolyer RA, Hayward NK, Samuels Y
  TITLE     Recurrent inactivating RASA2 mutations in melanoma.
  JOURNAL   Nat Genet. 2015 Dec;47(12):1408-10. doi: 10.1038/ng.3427. Epub 2015 
            Oct 26.
  PUBMED    26502337
REFERENCE   2  (bases 1 to 9156)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ng.3427"; 
            journalName: "Nat Genet"; date: "2015-12"; volume: "47"; issue: 
            "12"; pages: "1408-10"
COMMENT     Sequence Label: pCDF1-S400F_RASA2-EF1-Puro
FEATURES             Location/Qualifiers
     source          1..9156
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        116..319
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     promoter        1462..1665
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1662..1686
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      1736..1745
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1745..1768
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        4357..4568
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             4581..4849
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             4876..5475
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4876..4895)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5372..5392
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5482..6070
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5535..5555)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5953..5964)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    6407..6528
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6444..6463)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6498..6517
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6568..6703
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6736..6752)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6736..6752)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6749..6771)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6760..6776
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6784..6790,6791..6808,6809..6814))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6829..6850
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6967..6984)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7138..7726)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7218..7237)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7897..8688,8689..8757))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8520..8539
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8758..8862)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8930..8948
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8986..9008)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     9108..9127
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt
       61 atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat
      121 gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag
      181 tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc
      241 aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga
      301 ggtctatata agcagagctt gtgaaacttc gaggagtctc tttgttgagg acttttgagt
      361 tctcccttga ggctcccaca gatacaataa atatttgaga ttgaaccctg tcgagtatct
      421 gtgtaatctt ttttacctgt gaggtctcgg aatccgggcc gagaacttcg cagttggcgc
      481 ccgaacaggg acttgattga gagtgattga ggaagtgaag ctagagcaat agaaagctgt
      541 taagcagaac tcctgctgac ctaaataggg aagcagtagc agacgctgct aacagtgagt
      601 atctctagtg aagcagactc gagctcataa tcaagtcatt gtttaaaggc ccagataaat
      661 tacatctggt gactcttcgc ggaccttcaa gccaggagat tcgccgaggg acagtcaaca
      721 aggtaggaga gattctacag caacatgggg aatggacagg ggcgagattg gaaaatggcc
      781 attaagagat gtagtaatgt tgctgtagga gtagggggga agagtaaaaa atttggagaa
      841 gggaatttca gatgggccat tagaatggct aatgtatcta caggacgaga acctggtgat
      901 ataccagaga ctttagatca actaaggttg gttatttgcg atttacaaga aagaagagaa
      961 aaatttggat ctagcaaaga aattgatatg gcaattcctg cattgaggag aaatggtagg
     1021 caatgtggca tgtctgaaaa agaggaggaa tgatgaagta tctcagactt attttataag
     1081 ggagatactg tgctgagttc ttccctttga ggaaggtatg tcatatccta gacatagtct
     1141 caattttaaa agaagaggta ggataggagg gatggcccct tatgaattat tagcacaaca
     1201 agaatcctta agaatacaag attatttttc tgcaatacca caaaaattgc aagcacagtg
     1261 gatttattat aaagatcaaa aagataagaa atggaaagga ccaatgagag tagaatactg
     1321 gggacaggga tcagtattat taaaggatga agagaaggga tattttctta taatcgatac
     1381 tagtattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
     1441 ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag
     1501 cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt
     1561 tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa
     1621 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctcgttt agtgaaccgt
     1681 cagatcgcct ggagacgcca tccacgctgt tttgacctcc atagaagatt ctagagccac
     1741 catggattac aaggatgacg acgataaggc ggcggcggcg cctgctgctg cggcggcttc
     1801 ttccgaggcg ccagcggcga gtgcgactgc agagcccgag gccggggacc aggacagtcg
     1861 cgaggttcga gtgttgcaga gcctgcgggg caagatctgt gaagcaaaaa atttattgcc
     1921 atatcttgga ccccacaaaa tgagagattg tttctgtacc ataaatttgg accaggaaga
     1981 agtttatcgt acccaagttg tggaaaaatc tttaagccca tttttcagtg aagaatttta
     2041 ctttgagatt ccaagaactt tccagtattt gtctttctat gtttatgata agaatgtttt
     2101 acaaagagat ctccgtatag gaaaagtagc catcaaaaaa gaagacttgt gtaatcacag
     2161 tggcaaagaa acttggtttt cattacagcc tgttgactcc aattcagagg ttcagggtaa
     2221 agttcacctt gaattaaaac tgaatgaact gataacggag aatggaactg tatgccagca
     2281 gcttgttgta cacatcaagg catgccatgg gttgcctctc ataaatggcc aaagctgtga
     2341 cccttatgca acagtttctc tagtgggccc ttctaggaat gaccaaaaga agacaaaagt
     2401 aaagaagaaa acaagcaatc cgcagtttaa tgaaatcttt tattttgagg taaccagatc
     2461 cagtagttac accagaaagt cccagttcca ggtagaagag gaggacattg aaaagctaga
     2521 aatcaggatc gacttgtgga acaatggaaa cctagtccaa gatgttttcc taggtgagat
     2581 taaggttcct gtgaacgtat taagaactga ttcctctcat caagcctggt acttgctaca
     2641 gccaagagac aatggaaaca agtcatccaa aactgatgac ctggggtctc ttcgattaaa
     2701 tatatgttat acagaagact acgtgcttcc ttcagagtac tatggtcctt tgaaaacttt
     2761 gctgctaaaa tcaccagatg ttcaaccaat atctgcctca gctgcttaca ttttgagtga
     2821 aatatgtcga gataaaaatg atgctgtttt gccccttgta cgactgctgc tgcaccatga
     2881 taaacttgtt ccttttgcca ctgctgtggc tgaattagac ttgaaggata cacaagatgc
     2941 aaacacaatt tttagaggaa atttcctggc tacccgatgt ctggatgaga tgatgaaaat
     3001 agtgggaggg cactacctga aagtaacatt aaaacctatt cttgatgaga tatgtgactc
     3061 ctcaaaatcc tgtgaaatcg atcctattaa attgaaagag ggagataatg tagaaaataa
     3121 taaggagaat ctgcgctact atgtagacaa gttattcaat acaattgtaa aatcaagtat
     3181 gagctgcccc actgtaatgt gtgatatctt ttattctcta aggcagatgg ctactcagag
     3241 atttcctaat gaccctcatg ttcagtattc tgcagtgagc agctttgtat ttcttcgttt
     3301 ctttgctgta gccgtagtat cacctcatac ttttcatttg cgacctcatc atccagatgc
     3361 acagacaatt agaacattaa ctctcatctc aaaaactata caaactttgg gaagctgggg
     3421 gagtctgtcc aaaagcaagt caagtttcaa agagacattc atgtgtgaat ttttcaaaat
     3481 gtttcaagaa gaaggatata ttatagcagt taaaaagttc ttggatgaaa tttcatctac
     3541 tgaaactaaa gagtccagtg gtacgagtga gcctgtgcac ctgaaagaag gtgagatgta
     3601 taaaagagct caaggaagaa ctcggattgg aaaaaagaat tttaagaaac gatggttctg
     3661 cttaacaagc agagagctca cctaccacaa acagccaggc aaagatgcaa tctacacaat
     3721 cccagtaaaa aacattcttg ctgtggaaaa actggaagag agctctttca acaagaaaaa
     3781 tatgttccaa gtaatacata cggagaaacc actctatgtc caggcaaata actgtgtaga
     3841 agctaatgaa tggatagacg tactctgcag ggtgagccga tgcaatcaaa acaggctcag
     3901 tttttatcat ccctctgtgt atctgaacgg aaattggctc tgctgtcagg agactggtga
     3961 aaacactctc ggctgcaagc catgtactgc aggtgtccct gcagacatcc aaatagatat
     4021 tgatgaagac agagaaacag aaagaattta ttcccttttt accctcagtt tacttaagct
     4081 gcagaagatg gaagaggctt gtggaactat tgcagtctat caaggaccac agaaagagcc
     4141 tgatgattat tctaactttg taatcgagga ttctgtaaca acctttaaga caattcagca
     4201 aataaaaagc ataattgaga agctggatga acctcatgaa aaatatagga agaaaagatc
     4261 cagtagtgca aaatatggga gcaaggaaaa tccaattgtt gggaaagcat cttaggcggc
     4321 cgcgaaggat ctgcgatcgc tccggtgccc gtcagtgggc agagcgcaca tcgcccacag
     4381 tccccgagaa gttgggggga ggggtcggca attgaacggg tgcctagaga aggtggcgcg
     4441 gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag ggtgggggag
     4501 aaccgtatat aagtgcagta gtcgccgtga acgttctttt tcgcaacggg tttgccgcca
     4561 gaacacagct gaagcttcga ggggctcgca tctctccttc acgcgcccgc cgccctacct
     4621 gaggccgcca tccacgccgg ttgagtcgcg ttctgccgcc tcccgcctgt ggtgcctcct
     4681 gaactgcgtc cgccgtctag gtaagtttaa agctcaggtc gagaccgggc ctttgtccgg
     4741 cgctcccttg gagcctacct agactcagcc ggctctccac gctttgcctg accctgcttg
     4801 ctcaactcta cgtctttgtt tcgttttctg ttctgcgccg ttacagatcc aagctgtgac
     4861 cggcgcctac gctagatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac
     4921 gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac
     4981 accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg
     5041 cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc
     5101 tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg
     5161 gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg
     5221 caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag
     5281 ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg
     5341 gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc
     5401 ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc
     5461 aagcccggtg cctgagtcga caatcaacct ctggattaca aaatttgtga aagattgact
     5521 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     5581 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     5641 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     5701 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     5761 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     5821 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca
     5881 tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     5941 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     6001 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
     6061 gcctccccgc ctggtaccga tgacagagtt agaagatcgc ttcaggaagc tatttggcac
     6121 gacttctaca acgggagaca gcacagtaga ttctgaagat gaacctccta aaaaagaaaa
     6181 aagggtggac tgggatgagt attggaaccc tgaaatcgat agcttccagt gctttgtgaa
     6241 acttcgagga gtctctttgt tgaggacttt tgagttctcc cttgaggctc ccacagatac
     6301 aataaatatt tgagattgaa ccctgtcgag tatctgtgta atctttttta cctgtgaggt
     6361 ctcggaatcc gggccgagaa cttcgcagcg agctcattgt accgcgaact tgtttattgc
     6421 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     6481 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggct
     6541 ctagctatcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     6601 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
     6661 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagactt ttgcagagac
     6721 caaattcgta atcatgtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     6781 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     6841 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     6901 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     6961 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     7021 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     7081 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     7141 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     7201 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     7261 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     7321 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     7381 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     7441 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     7501 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     7561 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     7621 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     7681 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     7741 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     7801 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     7861 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     7921 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     7981 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     8041 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     8101 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     8161 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     8221 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     8281 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     8341 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     8401 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     8461 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     8521 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     8581 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     8641 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     8701 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     8761 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     8821 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     8881 gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat
     8941 cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca
     9001 gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca
     9061 gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca
     9121 gattgtactg agagtgcacc atatgccaag tacgcc
//
