LOCUS       sequence-241035-       12808 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Talin/Focal Adhesions, C1 Cloning Vector, 
            Excitation:434, Emission:474.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12808)
  TITLE     Michael Davidson Unpublished
REFERENCE   2  (bases 1 to 12808)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mTurquoise-Talin-C-18
FEATURES             Location/Qualifiers
     source          1..12808
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        169..472
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        473..676
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     627..647
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      715..724
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(721..723,724..726,727..1437)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2010)"
                     /label=mTurquoise
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(766..787)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1027..1046)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1374..1395
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     regulatory      1486..1495
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    9704..9825
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9741..9760)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9795..9814
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(9832..10287)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9969..9990)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     10181..10200
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        10314..10418
                     /gene="bla"
                     /label=AmpR promoter
     promoter        10420..10777
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        10420..10611
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(10443..10463)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      10628..10763
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             10812..11606
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             10812..11606
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(10866..10885)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     11476..11495
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(11794..11813)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    11838..11885
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      12214..12802
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12703..12722
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
       61 cctgcgttat cccctgattc tgtggataac cgtattaccg ccatgcatta gttattaata
      121 gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact
      181 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat
      241 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta
      301 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc
      361 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg
      421 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg
      481 gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct
      541 ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa
      601 atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt
      661 ctatataagc agagctggtt tagtgaaccg tcagatccgc tagcgctacc ggtcgccacc
      721 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
      781 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
      841 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
      901 ctcgtgacca ccctgtcctg gggcgtgcag tgcttcgccc gctaccccga ccacatgaag
      961 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
     1021 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
     1081 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
     1141 aagctggagt acaactacat cagcgacaac gtctatatca ccgccgacaa gcagaagaac
     1201 ggcatcaagg ccaacttcaa gatccgccac aacatcgagg acggcggcgt gcagctcgcc
     1261 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
     1321 tacctgagca cccagtccaa gctgagcaaa gaccccaacg agaagcgcga tcacatggtc
     1381 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtcc
     1441 ggactcagat ctcgagctca agctagcttc gaattcgcag aagctgccac catggttgcg
     1501 ctttcgctga agattagcat tgggaatgtg gtgaagacga tgcaatttga gccatctacc
     1561 atggtgtacg acgcctgccg catgattcgt gagcggatcc cagaggccct ggctggccct
     1621 cccaacgact ttgggctctt tctgtcagat gatgacccca aaaaaggcat ctggctagag
     1681 gctgggaaag ctttggacta ctacatgctc cgaaatgggg acaccatgga gtacagaaaa
     1741 aagcagagac ccctgaagat acggatgtta gacggaacgg tgaagactat catggtggat
     1801 gactccaaga ccgtcacaga catgctcatg accatttgtg cccgaattgg tatcaccaac
     1861 catgatgagt attcactggt tcgagagctg atggaagaaa agaaagatga ggggacaggg
     1921 accctgagaa aagacaagac cctactgcga gatgaaaaga agatggagaa gctaaagcag
     1981 aagttgcaca cagacgatga gttgaactgg ctggaccatg ggcggacact gagggaacag
     2041 ggagtggaag agcatgagac gttgctgctg cggagaaagt tcttctactc agaccagaat
     2101 gtggattccc gagaccctgt acagctgaac cttctctatg tgcaggcacg agatgacatc
     2161 ctgaatggct cccatcctgt ctcctttgac aaggcctgtg aatttgcagg cttccagtgc
     2221 cagatccagt ttggacctca caatgaacag aagcacaagg ctggcttcct tgacctgaag
     2281 gacttcctgc ccaaggagta tgtgaagcag aagggagagc gtaagatctt tcaggcacac
     2341 aagaattgtg ggcagatgag tgaaattgag gccaaggtac gctacgtgaa gctggcccgt
     2401 tccctcaaga cttacggtgt ctccttcttc ctagtcaagg aaaagatgaa ggggaagaat
     2461 aaactggtgc ccaggctgtt gggcatcact aaggagtgtg tgatgcgtgt ggatgagaag
     2521 accaaggagg tgattcagga atggagcctc accaacatca aacgctgggc tgcctctccc
     2581 aagagcttta ctctggactt tggagactat caggatggct actactcagt acagacgaca
     2641 gaaggcgagc agatcgcaca gctcattgct ggctacatag atatcatcct taagaagaaa
     2701 aaaagcaagg accattttgg gctggaggga gatgaagagt ctactatgtt ggaggactca
     2761 gtgtctccca aaaagtcaac agtccttcag cagcagtaca accgagtggg gaaagtggag
     2821 cacggctctg tggctctgcc agccatcatg cgctctggag cctctggtcc tgagaatttc
     2881 caggtgggga gcatgccacc tgcccagcag cagatcacca gtggccagat gcaccgagga
     2941 cacatgccac ctctgacttc agcccagcag gcgctcactg gaaccattaa ctccagcatg
     3001 caggctgtgc aggctgccca ggccactctg gatgactttg agactctacc ccctcttggc
     3061 caggatgctg cctccaaagc ttggcgtaag aacaagatgg atgaatcaaa gcatgagatc
     3121 cactcccagg tagatgccat cacagctggc actgcctctg tggtaaacct gacagcagga
     3181 gatcctgcag agacagacta tacagcagtg ggctgtgcag tcaccaccat ctcttccaac
     3241 ctgacggaga tgtcccgtgg ggtgaagcta ttggctgccc tgctggagga tgaaggcggc
     3301 aatggccggc cccttctgca agcagcaaag ggccttgcag gggctgtgtc ggaactgctt
     3361 cgcagtgctc agcccgccag cgctgagcct cgtcagaacc tgctgcaggc agccgggaac
     3421 gtgggccagg ccagtgggga gctgttgcag caaattgggg aaagtgacac tgacccccac
     3481 ttccaggatg ttctaatgca gctagcaaac gcagtggcaa gtgctgcagc cgccctggtc
     3541 ctcaaggcca agagtgtggc ccagcgcaca gaggattccg ggcttcagac ccaagtgatt
     3601 gctgcagcta cgcaatgtgc tctgtccact tcccagctgg tggcctgtac caaggtggta
     3661 gcacctacaa tcagctcacc tgtgtgccaa gaacagctag tcgaggctgg gcgactggtg
     3721 gccaaagctg tggagggctg tgtgtctgcc tcgcaagcag ctacagaaga tggacagctg
     3781 cttcgagggg taggagcagc agccacggct gtcacccagg ccctcaatga gctgctgcag
     3841 cacgtgaagg cccacgccac cggagctggg cctgccggtc ggtatgacca agccactgat
     3901 accatcctca ccgtcactga aaacatcttc agctccatgg gtgatgctgg ggagatggtg
     3961 cgccaggccc gcatcctggc ccaagccacc tcagacctgg tcaatgctat caaggccgat
     4021 gctgaggggg agagtgatct ggagaactct agaaagctcc tgagtgctgc caagatcctc
     4081 gctgatgcca ccgccaagat ggtggaggcg gccaagggag cagccgccca ccctgacagt
     4141 gaggaacagc agcagcgact gcgtgaagca gctgaggggc ttcgcatggc caccaatgca
     4201 gctgcgcaga acgccatcaa gaagaagttg gtgcagcgcc tggagcatgc agccaagcaa
     4261 gctgcagcct ctgcaacaca gaccattgct gcagcccaac atgcagcctc tgcccccaag
     4321 gcctctgcgg gcccccagcc cctactggtg cagagctgta aggccgtggc agagcagatt
     4381 ccgttgctgg tgcagggtgt ccgaggaagt caagctcagc ctgacagccc tagtgctcag
     4441 ctcgccctca ttgctgccag ccagagcttc ctgcagccag gtgggaagat ggtggcagcg
     4501 gcaaaagcct cagtgccaac aattcaggac caggcttcag cgatgcagct gagtcagtgc
     4561 gcaaagaacc taggcactgc cctggccgag ctgcgcactg ctgctcagaa ggctcaggag
     4621 gcatgtggac ctttggagat ggattctgca ctgagtgttg tacaaaatct agagaaagac
     4681 ctacaggaaa taaaagcagc agctcgagat ggcaagctta agcccttacc tggggaaaca
     4741 atggagaagt gtacccaaga tcttggcaac agcaccaaag cggtgagctc tgccatcgcc
     4801 aagctgctgg gggagattgc ccagggcaac gagaactatg caggtattgc agctcgggat
     4861 gtagctggtg gactaaggtc actagcccag gctgcgcggg gtgtggctgc gctgacatca
     4921 gatcctgcag tgcaggccat tgtgcttgac acagccagcg acgttctgga caaggccagc
     4981 agcctcattg aagaggcaaa aaaagcatct ggacacccag gggacccaga aagccagcaa
     5041 aggcttgctc aggtagctaa agcagtgacc caagccctga accgctgtgt cagctgcctg
     5101 cctggccaaa gagacgtgga taatgcccta cgagccgttg gagatgccag caagcgactc
     5161 ctgagtgact tgcttcctcc aagcacgggg acatttcaag aagcacagag ccgattgaat
     5221 gaagctgcag ctgggttaaa tcaggccgcc acagaactgg tgcaagcctc ccgaggaact
     5281 cctcaggacc tggctcgggc ctccggccga tttggacagg acttcagcac cttcctggaa
     5341 gctggtgtgg agatggccgg acaggccccg agccaggagg accgagccca ggtggtgtcc
     5401 aacctgaagg gcatatccat gtcttcaagc aaacttctcc ttgccgctaa ggccttgtcc
     5461 acagaccctg cttctcccaa cctcaagagt cagctggctg cggctgcccg ggcagtgacg
     5521 gacagcatca accagctcat caccatgtgc acccagcagg cacctggcca gaaggagtgt
     5581 gacaatgcac ttcggcagct agagacagtc cgagaactcc tggagaatcc agtccagccc
     5641 atcaacgaca tgtcctactt cggttgcttg gacagtgtca tggagaactc caaggtccta
     5701 ggtgaggcca tgactggcat ctcccaaaat gccaagaatg gaaatctgcc ggagtttgga
     5761 gatgccattg ccacagcctc caaggctctc tgtggcttca cggaggcagc cgcacaggca
     5821 gcatatctag ttggtgtctc tgaccccaac agccaagctg gacagcaagg actggtggaa
     5881 cccacacagt ttgcccgtgc aaaccaggca attcagatgg cctgtcagag tctgggggag
     5941 cctggctgta cccaggccca ggtgttatct gcagccacta ttgtagccaa acacacatct
     6001 gcattgtgta acagctgtcg cctggcttcc gctaggactg ccaatcccac tgccaagcgc
     6061 cagtttgtac agtcagccaa ggaggtggcc aacagtacag ccaatcttgt caagaccatc
     6121 aaggcactag atggggactt cacagaagag aaccgtgccc agtgccgagc agccacagcc
     6181 cctctgctgg aagctgtgga taacctgagt gcctttgcct ccaaccctga gttctccagc
     6241 gtccctgccc agatcagccc tgagggcaga gcagccatgg agcccattgt aatctctgct
     6301 aagacaatgt tggagagtgc tggagggctc atccagacag ctcgggcgtt agcagtcaat
     6361 ccccgagacc ccccacgttg gtctgtgcta gctggccact ctcggactgt ctcagattcc
     6421 atcaagaaac ttattacaag catgagggac aaagccccag ggcagctgga gtgtgagaca
     6481 gccattgcgg ctctgaacag ctgcttgcgg gacctagatc aggcttcgct tgccgctgtc
     6541 agccagcagc ttgctccccg tgaaggaatc tctcaagagg ctttgcacac ccagatgctc
     6601 actgcagtgc aagaaatttc tcatctcatt gagccgctgg ccagcgctgc tcgggctgaa
     6661 gcctcccagc tgggacacaa ggtgtcccaa atggcccagt actttgaacc actcaccctg
     6721 gctgcagtgg gtgctgcgtc taagaccctg agccacccgc agcagatggc acttctggac
     6781 cagactaaaa cgttggcaga gtctgccttg cagttgctat atactgccaa ggaggctggt
     6841 ggtaacccca agcaagcagc acacacccag gaagccctgg aggaggctgt gcagatgatg
     6901 acagaggccg tagaagacct gacgacgacc ctcaacgaag cagccagtgc tgcaggagtc
     6961 gttggcggca tggtggactc tatcactcag gccatcaacc agctagatga aggacctatg
     7021 ggtgacccag aaggctcatt cgtagattac cagacaacca tggtgaggac agccaaggcc
     7081 attgctgtca ctgttcagga gatggtaacc aagtcaaaca ccagccctga agagctaggc
     7141 cctcttgcca accagctgac cagtgactat ggccgactgg cctcacaagc caagcctgca
     7201 gctgtggctg ctgaaaatga agagataggc gctcacatca aacaccgagt acaggagctg
     7261 ggccatggct gctctgctct ggtcaccaag gcaggtgcct tgcagtgtag ccccagtgat
     7321 gtctacacca agaaggagct catagagtgt gcccgcagag tgtcagagaa ggtctcccat
     7381 gttctggctg cactccaggc tgggaatcgt ggtacccagg cctgcattac agcagccagt
     7441 gctgtgtctg gtatcattgc tgacctcgac accaccatca tgttcgctac tgctggcaca
     7501 cttaaccgtg agggtgctga aacttttgct gaccaccggg agggtatctt aaagacagca
     7561 aaggttcttg tggaggacac caaggtccta gtgcagaatg cagctgggag ccaggagaag
     7621 ttggcacaag ccgcccagtc ctccgtggcc accattaccc gcctcgctga tgtggtcaag
     7681 ctcggtgcag ccagcctagg agccgaagac cctgaaactc aggtggtgct gatcaatgca
     7741 gtaaaggacg tagccaaggc cctgggtgac ctcatcagcg ctacgaaggc tgcagcgggc
     7801 aaagttgggg atgaccctgc agtgtggcag ctcaagaact ctgccaaggt gatggtgacc
     7861 aatgtgacat cattgctcaa gacagtgaag gctgtggaag atgaggccac caaaggcaca
     7921 cgggccctag aggcaaccac agagcacata cgtcaggaac tggcggtctt ctgttcccca
     7981 gagccacctg ccaagacctc tacccctgaa gacttcatcc gaatgaccaa gggtattact
     8041 atggcaacag ccaaagccgt tgctgctggc aattcctgtc gacaggaaga tgtcattgcc
     8101 acagccaatc tgagccgacg ggctattgcg gacatgcttc gggcttgcaa ggaagcagct
     8161 ttccacccag aagtggcgcc tgatgtacgg ctccgagccc tgcactatgg ccgggagtgt
     8221 gccaatggtt acctggaact gctggaccac gtgctgctga cccttcagaa gccaaaccca
     8281 gacctgaagc agcagctaac tggacactca aagcgggttg ctggctctgt gactgagctc
     8341 atccaggctg ctgaagccat gaaaggaaca gagtgggtgg acccagagga ccctactgtc
     8401 attgctgaga atgagctctt gggagccgca gccgccatcg aggctgcagc caagaagctg
     8461 gagcagctga agcccagggc caaacccaag gaggcggatg agtccttgaa ctttgaggaa
     8521 caaatcctag aagctgccaa gtccatcgct gcagccacca gtgcactggt aaaggctgcg
     8581 tcagcagccc agagggagct ggtggctcaa ggaaaggtgg gcgccattcc agccaatgca
     8641 ctggatgatg ggcagtggtc gcagggcctc atttccgctg cccgtatggt ggctgcggcc
     8701 accaacaatc tgtgtgaagc agctaacgca gctgtccagg gccatgctag ccaggagaaa
     8761 ctcatttcct cagccaagca agtagctgcc tccacagctc agctcctggt agcttgcaag
     8821 gtcaaagcag atcaggactc tgaggcaatg aaacggctcc aggctgctgg caatgcagtg
     8881 aagagggcct cagataacct ggtaaaggcg gcccagaaag ctgcagcctt cgaagaccag
     8941 gagaatgaga cggtggtggt gaaggagaaa atggttgggg gcattgccca gattatcgca
     9001 gcacaggaag agatgcttcg gaaggaacga gagctggaag aggctcggaa aaagctcgcc
     9061 cagatccggc agcagcagta caagttcttg ccttcagagc ttcgagacga gcactaaaaa
     9121 agccctgtgt atttaatgca gacccagccc agagactgtg cctgccacta ccaaagcctt
     9181 ctgggctgct caggacccaa gctgcccaac cccagcactc ccccaaagtg cctgccaaac
     9241 cctgggcctg gccctgccca gtcccactgc aagccgtgtc ctctccctga ctcccaagtg
     9301 cctttgcacc ctagggcccc taagtgcctg cccccctcca gagtattaac gctccaagag
     9361 tattattaat gctgccgtac ctcagtttga acctgccagg gccccagctg ctccagcctg
     9421 ccagcagctt ccagccagtc cccacggcca cgtcagctca actcatccct ttttgatact
     9481 atatccccta cccagctacc tatggggctt gagggttgta aacccaaaca ggtcagactc
     9541 ccggaattct gcagtcgacg gtaccgcggg cccgggatcc accggatcta gataactgat
     9601 cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct
     9661 ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc
     9721 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     9781 actgcattct agttgtggtt tgtccaaact catcaatgta tcttaacgcg taaattgtaa
     9841 gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc
     9901 aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga
     9961 gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag
    10021 ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt
    10081 ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta
    10141 gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag
    10201 cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg
    10261 cgcttaatgc gccgctacag ggcgcgtcag gtggcacttt tcggggaaat gtgcgcggaa
    10321 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
    10381 cctgataaat gcttcaataa tattgaaaaa ggaagagtcc tgaggcggaa agaaccagct
    10441 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
    10501 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
    10561 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
    10621 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
    10681 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
    10741 gtgaggaggc ttttttggag gcctaggctt ttgcaaagat cgatcaagag acaggatgag
    10801 gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg
    10861 agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt
    10921 tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc
    10981 tgaatgaact gcaagacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt
    11041 gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag
    11101 tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg
    11161 ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag
    11221 cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg
    11281 atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcga
    11341 gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca
    11401 tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc
    11461 gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg
    11521 ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct
    11581 atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc
    11641 gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg
    11701 cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct
    11761 ggagttcttc gcccacccta gggggaggct aactgaaaca cggaaggaga caataccgga
    11821 aggaacccgc gctatgacgg caataaaaag acagaataaa acgcacggtg ttgggtcgtt
    11881 tgttcataaa cgcggggttc ggtcccaggg ctggcactct gtcgataccc caccgagacc
    11941 ccattggggc caatacgccc gcgtttcttc cttttcccca ccccaccccc caagttcggg
    12001 tgaaggccca gggctcgcag ccaacgtcgg ggcggcaggc cctgccatag cctcaggtta
    12061 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
    12121 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
    12181 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
    12241 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
    12301 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
    12361 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
    12421 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
    12481 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
    12541 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
    12601 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
    12661 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
    12721 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
    12781 aggggggcgg agcctatgga aaaacgcc
//
