LOCUS       sequence-241041-        9392 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Dox-inducible GFP (N-terminal tag) SCAF4 expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9392)
  AUTHORS   Gregersen LH, Mitter R, Ugalde AP, Nojima T, Proudfoot NJ, Agami R, 
            Stewart A, Svejstrup JQ
  TITLE     SCAF4 and SCAF8, mRNA Anti-Terminator Proteins.
  JOURNAL   Cell. 2019 May 10. pii: S0092-8674(19)30457-X. doi: 
            10.1016/j.cell.2019.04.038.
  PUBMED    31104839
REFERENCE   2  (bases 1 to 9392)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2019 
            May 10. pii: S0092-8674(19)30457-X. doi: 
            10.1016/j.cell.2019.04.038."
COMMENT     Sequence Label: pFRT/TO/GFP-SCAF4 (CRISPR_resistant)
FEATURES             Location/Qualifiers
     source          1..9392
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     164..185
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    234..258
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3709..3733)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3786..3827
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(3865..3882)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3871..4095
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(4228..4247)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    4379..4426
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             4434..5456
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             4434..5456
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    5586..5707
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5623..5642)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5677..5696
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5756..5772)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5756..5772)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5769..5791)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5780..5796
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5804..5810,5811..5828,5829..5834))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5849..5870
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5987..6004)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6158..6746)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6238..6257)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6917..7708,7709..7777))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7540..7559
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7778..7882)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7957..7976)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        8148..8527
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8528..8731
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8682..8702
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    8733..8751
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8754..8772
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     8782..8806
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(8903..8905,8906..8908,8909..9392,1..227)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(8903..8905,8906..8908,8909..9392,1..227)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8948..8969)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(9209..9228)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc
       61 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca
      121 cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
      181 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagaag cttacaagtt
      241 tgtacaaaaa agcaggcttc accatggacg ccgtcaacgc cttcaaccag gagctctttt
      301 cgcttatgga tatgaaacct cccatctcta gagccaagat gattctcatc actaaagctg
      361 ctattaaagc tattaagctt tataagcatg tagttcaaat agtagaaaag ttcatcaaaa
      421 agtgtaaacc agaatacaag gttccgggat tatatgtaat tgactcaatt gtgcgacagt
      481 ctcgtcatca gtttggaact gataaagatg tttttgggcc aagattctct aaaaacataa
      541 ctgccacatt ccaatattta tatctttgtc catctgaaga taagagtaaa atagttcgtg
      601 tgctgaacct ttggcaaaaa aatggagtgt tcaaaattga aattattcaa cctcttttgg
      661 acatggcagc gggaaccagt aatgcagccc cagtagcaga aaatgttacc aataatgaag
      721 gctcacctcc acctccagta aaagtttctt ctgaacctcc cacacaagcc actccaaact
      781 ccgtgcctgc agttcctcaa ttacccagct ctgatgcttt tgctgctgtg gctcaactct
      841 tccaaactac acagggccaa cagcttcagc agatccttca gacttttcaa cagcctccaa
      901 aaccacagtc tcctgccctt gacaatgctg tgatggctca ggttcaggct atcacagctc
      961 agttaaagac aactcctaca caaccatctg aacaaaaagc tgctttcccc ccacctgaac
     1021 agaaaactgc atttgacaag aagttgcttg atagatttga ctatgatgat gagccagaag
     1081 ctgtggaaga atcaaagaaa gaggatacca ctgccgtcac cacgacagca cctgctgccg
     1141 cagtaccccc tgcacccacc gccaccgtgc ctgctgctgc tgcacccgct gctgcctctc
     1201 ctcctcctcc acaggcacca tttggctttc ctggagatgg catgcagcag cctgcttata
     1261 cgcaacacca gaatatggat caattccaac ctcgtatgat gggaatacaa caggatccaa
     1321 tgcaccatca ggttccactt cctcctaatg gacaaatgcc aggatttgga cttcttccta
     1381 cacctccatt tcctcccatg gctcagcctg tgattcctcc aactccacca gtgcagcagc
     1441 ctttccaagc ttcttttcag gcacaaaatg aaccacttac acagaagccg catcagcagg
     1501 aaatggaagt agaacaacct tgtattcaag aggttaagcg acatatgtct gataacagaa
     1561 agtcaagatc taggtcagca tccaggtcac caaaaaggag gcgatctaga tctggttcta
     1621 gatctcgaag gtctcggcat cgacgttctc gatctcggtc cagggataga cgccgacatt
     1681 ctccccgatc tcgatctcaa gaaagacggg atcgagaaaa agagagagaa cgtcgacaaa
     1741 aaggcctccc tcaagtgaaa ccggaaactg caagtgtttg cagtactacc ctctgggtgg
     1801 ggcagctgga caaaagaact actcagcagg atgttgccag tctcttggaa gagtttggtc
     1861 caattgaatc aattaatatg attcctccca ggggttgtgc ctatattgtt atggttcata
     1921 ggcaagatgc ctatcgtgcc ctgcagaaac tgagccgagg aaactataaa gtgaaccaga
     1981 aatccataaa gattgcctgg gccttaaaca aaggaataaa ggcagattat aagcagtatt
     2041 gggatgtaga acttggtgtt acttatattc catgggacaa agtcaagcct gaggaactgg
     2101 agagtttttg tgaaggagga atgttggaca gtgacacact taacccagat tggaaaggaa
     2161 ttcctaagaa gcctgaaaat gaagttgctc aaaatggagg tgctgaaacc tcacacacag
     2221 aaccagtatc acccatacct aaaccattac ctgtgcctgt ccctcctatt cctgttcctg
     2281 cacctataac agtgccacct ccacaggtcc caccacatca accgggtcca cctgtagttg
     2341 gtgctctcca gccgcctgct ttcacgcctc ctctgggaat accgcctcca ggctttggtc
     2401 ctggtgttcc tcctccccct cctcctccac catttttgcg cccaggattc aacccaatgc
     2461 atttaccacc aggttttctg cctcctggac ccccacctcc tataactcca ccagtatcca
     2521 ttcctcctcc tcacactcca ccaataagca tcccaaactc tactatcgct ggtataaatg
     2581 aagacactac aaaagactta tctattggaa atcccattcc aacagtggtg tctggggcta
     2641 gaggaaacgc cgagtctggt gacagcgtga aaatgtatgg ctctgccgtg ccacctgctg
     2701 cacccacgaa tctgcccacc cctcctgtaa cccagcctgt ttcacttctt ggcactcaag
     2761 gagttgcccc tggtcctgta attggacttc aggcaccatc tactggtctt cttggcgccc
     2821 ggcccggtct catcccactc cagcgccctc caggaatgcc cccacctcac ttacagcggt
     2881 tccctttgat gccgccccgt cccatgccac cgcacatgat gcacagaggc ccaccgccag
     2941 gaccaggggg ctttgcgatg cctccacctc atggaatgaa aggtcccttc ccaccgcatg
     3001 gcccctttgt taggcctggt ggaatgccag ggctcggggg cccagggcca ggcccagggg
     3061 gtcctgaaga cagagacgga aggcaacagc cgccgcagca gccacagcag cagccacagc
     3121 cgcaggcgcc ccagcaacca cagcagcagc agcagcagca gccaccacca tcacaacagc
     3181 ctccaccaac acagcagcag ccacagcagt ttagaaatga taacaggcag cagttcaatt
     3241 caggtagaga ccaagaaagg tttggaagaa gatcttttgg aaatagggtg gaaaatgacc
     3301 gggaacggta tgggaaccgt aatgatgata gagataatag taaccgtgac aggagagagt
     3361 ggggaaggag gagccctgac cgggacaggc acagagactt ggaagagaga aatagacgct
     3421 ctagtgggca tcgagacaga gagagagatt ctagagatag agagtctcgt agagagaagg
     3481 aagaagcccg aggaaaggaa aagcctgagg tgacagacag ggcaggtggt aacaaaaccg
     3541 ttgaacctcc cattagccaa gtgggaaatg tagacactgc ttcagaactt gagaaggggg
     3601 tgtctgaggc tgcagtccta aagccttctg aagagttacc tgctgaggct acctcatccg
     3661 ttgaacccga aaaggattct ggctcagcag cagaggctcc tcgttaaaac ccagctttct
     3721 tgtacaaagt ggttgatatc cagcacagtg gcggccgctc aagtctagag ggcccgcggt
     3781 tcgaaggtaa gcctatccct aaccctctcc tcggtctcga ttctacgctc gagtctagag
     3841 ggcccgttta aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg
     3901 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct
     3961 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg
     4021 gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg
     4081 cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc
     4141 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     4201 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     4261 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     4321 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtacctaga
     4381 agttcctatt ccgaagttcc tattctctag aaagtatagg aacttccttg gccaaaaagc
     4441 ctgaactcac cgcgacgtct gtcgagaagt ttctgatcga aaagttcgac agcgtctccg
     4501 acctgatgca gctctcggag ggcgaagaat ctcgtgcttt cagcttcgat gtaggagggc
     4561 gtggatatgt cctgcgggta aatagctgcg ccgatggttt ctacaaagat cgttatgttt
     4621 atcggcactt tgcatcggcc gcgctcccga ttccggaagt gcttgacatt ggggaattca
     4681 gcgagagcct gacctattgc atctcccgcc gtgcacaggg tgtcacgttg caagacctgc
     4741 ctgaaaccga actgcccgct gttctgcagc cggtcgcgga ggccatggat gcgatcgctg
     4801 cggccgatct tagccagacg agcgggttcg gcccattcgg accgcaagga atcggtcaat
     4861 acactacatg gcgtgatttc atatgcgcga ttgctgatcc ccatgtgtat cactggcaaa
     4921 ctgtgatgga cgacaccgtc agtgcgtccg tcgcgcaggc tctcgatgag ctgatgcttt
     4981 gggccgagga ctgccccgaa gtccggcacc tcgtgcacgc ggatttcggc tccaacaatg
     5041 tcctgacgga caatggccgc ataacagcgg tcattgactg gagcgaggcg atgttcgggg
     5101 attcccaata cgaggtcgcc aacatcttct tctggaggcc gtggttggct tgtatggagc
     5161 agcagacgcg ctacttcgag cggaggcatc cggagcttgc aggatcgccg cggctccggg
     5221 cgtatatgct ccgcattggt cttgaccaac tctatcagag cttggttgac ggcaatttcg
     5281 atgatgcagc ttgggcgcag ggtcgatgcg acgcaatcgt ccgatccgga gccgggactg
     5341 tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg gaccgatggc tgtgtagaag
     5401 tactcgccga tagtggaaac cgacgcccca gcactcgtcc gagggcaaag gaatagcacg
     5461 tactacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt
     5521 tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc
     5581 accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     5641 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     5701 tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat
     5761 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     5821 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
     5881 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     5941 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     6001 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     6061 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     6121 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     6181 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     6241 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     6301 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     6361 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     6421 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     6481 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     6541 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     6601 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     6661 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     6721 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     6781 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     6841 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     6901 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     6961 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     7021 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     7081 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     7141 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     7201 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     7261 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     7321 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     7381 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     7441 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     7501 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     7561 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     7621 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     7681 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     7741 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     7801 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     7861 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgacggat
     7921 cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg atgccgcata
     7981 gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa
     8041 aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc tgcttagggt
     8101 taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac attgattatt
     8161 gactagttat taatagtaat caattacggg gtcattagtt catagcccat atatggagtt
     8221 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc
     8281 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg
     8341 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
     8401 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
     8461 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
     8521 taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg
     8581 gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca
     8641 acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg
     8701 tgtacggtgg gaggtctata taagcagagc tctccctatc agtgatagag atctccctat
     8761 cagtgataga gatcgtcgac gagctcgttt agtgaaccgt cagatcgcct ggagacgcca
     8821 tccacgctgt tttgacctcc atagaagaca ccgggaccga tccagcctcc ggactctagc
     8881 gtttaaactt aagcttggta ccatggtgag caagggcgag gagctgttca ccggggtggt
     8941 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga
     9001 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa
     9061 gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag
     9121 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
     9181 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt
     9241 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
     9301 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat
     9361 catggccgac aagcagaaga acggcatcaa gg
//
