LOCUS       sequence-241042-       12662 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of MET.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12662)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 12662)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-MET
FEATURES             Location/Qualifiers
     source          1..12662
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2223..3401
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2454..3392
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3349..3369
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    3421..3445
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(7622..7646)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        7654..7672
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7697..7716)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7698..7716)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(7722..7742)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             7796..7837
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    7858..8425
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    7875..8427
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(8019..8036)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     8246..8265
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             8439..9038
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8439..8458)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8935..8955
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9052..9640
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9105..9125)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9523..9534)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9715..9948
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(10067..10085)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10153..10257
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10258..10326,10327..11118)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10476..10495)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11289..11877
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11778..11797
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12031..12048
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12288..12309
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12324..12329,12330..12347,12348..12354)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12362..12378
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12367..12389
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12386..12402
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12386..12402
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12452..12471
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccct gcagatatca
     3421 acaagtttgt acaaaaaagc aggcaccatg aaggcccccg ctgtgcttgc acctggcatc
     3481 ctcgtgctcc tgtttacctt ggtgcagagg agcaatgggg agtgtaaaga ggcactagca
     3541 aagtccgaga tgaatgtgaa tatgaagtat cagcttccca acttcaccgc ggaaacaccc
     3601 atccagaatg tcattctaca tgagcatcac attttccttg gtgccactaa ctacatttat
     3661 gttttaaatg aggaagacct tcagaaggtt gctgagtaca agactgggcc tgtgctggaa
     3721 cacccagatt gtttcccatg tcaggactgc agcagcaaag ccaatttatc aggaggtgtt
     3781 tggaaagata acatcaacat ggctctagtt gtcgacacct actatgatga tcaactcatt
     3841 agctgtggca gcgtcaacag agggacctgc cagcgacatg tctttcccca caatcatact
     3901 gctgacatac agtcggaggt tcactgcata ttctccccac agatagaaga gcccagccag
     3961 tgtcctgact gtgtggtgag cgccctggga gccaaagtcc tttcatctgt aaaggaccgg
     4021 ttcatcaact tctttgtagg caataccata aattcttctt atttcccaga tcatccattg
     4081 cattcgatat cagtgagaag gctaaaggaa acgaaagatg gttttatgtt tttgacggac
     4141 cagtcctaca ttgatgtttt acctgagttc agagattctt accccattaa gtatgtccat
     4201 gcctttgaaa gcaacaattt tatttacttc ttgacggtcc aaagggaaac tctagatgct
     4261 cagacttttc acacaagaat aatcaggttc tgttccataa actctggatt gcattcctac
     4321 atggaaatgc ctctggagtg tattctcaca gaaaagagaa aaaagagatc cacaaagaag
     4381 gaagtgttta atatacttca ggctgcgtat gtcagcaagc ctggggccca gcttgctaga
     4441 caaataggag ccagcctgaa tgatgacatt cttttcgggg tgttcgcaca aagcaagcca
     4501 gattcggccg aaccaatgga tcgatctgcc atgtgtgcat tccctatcaa atatgtcaac
     4561 gacttcttca acaagatcgt caacaaaaac aatgtgagat gtctccagca tttttacgga
     4621 cccaatcatg agcactgctt taataggaca cttctgagaa attcatcagg ctgtgaagcg
     4681 cgccgtgatg aatatcgaac agagtttacc acagctttgc agcgcgttga cttattcatg
     4741 ggtcaattca gcgaagtcct cttaacatct atatccacct tcattaaagg agacctcacc
     4801 atagctaatc ttgggacatc agagggtcgc ttcatgcagg ttgtggtttc tcgatcagga
     4861 ccatcaaccc ctcatgtgaa ttttctcctg gactcccatc cagtgtctcc agaagtgatt
     4921 gtggagcata cattaaacca aaatggctac acactggtta tcactgggaa gaagatcacg
     4981 aagatcccat tgaatggctt gggctgcaga catttccagt cctgcagtca atgcctctct
     5041 gccccaccct ttgttcagtg tggctggtgc cacgacaaat gtgtgcgatc ggaggaatgc
     5101 ctgagcggga catggactca acagatctgt ctgcctgcaa tctacaaggt tttcccaaat
     5161 agtgcacccc ttgaaggagg gacaaggctg accatatgtg gctgggactt tggatttcgg
     5221 aggaataata aatttgattt aaagaaaact agagttctcc ttggaaatga gagctgcacc
     5281 ttgactttaa gtgagagcac gatgaataca ttgaaatgca cagttggtcc tgccatgaat
     5341 aagcatttca atatgtccat aattatttca aatggccacg ggacaacaca atacagtaca
     5401 ttctcctatg tggatcctgt aataacaagt atttcgccga aatacggtcc tatggctggt
     5461 ggcactttac ttactttaac tggaaattac ctaaacagtg ggaattctag acacatttca
     5521 attggtggaa aaacatgtac tttaaaaagt gtgtcaaaca gtattcttga atgttatacc
     5581 ccagcccaaa ccatttcaac tgagtttgct gttaaattga aaattgactt agccaaccga
     5641 gagacaagca tcttcagtta ccgtgaagat cccattgtct atgaaattca tccaaccaaa
     5701 tcttttatta gtggtgggag cacaataaca ggtgttggga aaaacctgaa ttcagttagt
     5761 gtcccgagaa tggtcataaa tgtgcatgaa gcaggaagga actttacagt ggcatgtcaa
     5821 catcgctcta attcagagat aatctgttgt accactcctt ccctgcaaca gctgaatctg
     5881 caactccccc tgaaaaccaa agcctttttc atgttagatg ggatcctttc caaatacttt
     5941 gatctcattt atgtacataa tcctgtgttt aagccttttg aaaagccagt gatgatctca
     6001 atgggcaatg aaaatgtact ggaaattaag ggaaatgata ttgaccctga agcagttaaa
     6061 ggtgaagtgt taaaagttgg aaataagagc tgtgagaata tacacttaca ttctgaagcc
     6121 gttttatgca cggtccccaa tgacctgctg aaattgaaca gcgagctaaa tatagagtgg
     6181 aagcaagcaa tttcttcaac cgtccttgga aaagtaatag ttcaaccaga tcagaatttc
     6241 acaggattga ttgctggtgt tgtctcaata tcaacagcac tgttattact acttgggttt
     6301 ttcctgtggc tgaaaaagag aaagcaaatt aaagatctgg gcagtgaatt agttcgctac
     6361 gatgcaagag tacacactcc tcatttggat aggcttgtaa gtgcccgaag tgtaagccca
     6421 actacagaaa tggtttcaaa tgaatctgta gactaccgag ctacttttcc agaagatcag
     6481 tttcctaatt catctcagaa cggttcatgc cgacaagtgc agtatcctct gacagacatg
     6541 tcccccatcc taactagtgg ggactctgat atatccagtc cattactgca aaatactgtc
     6601 cacattgacc tcagtgctct aaatccagag ctggtccagg cagtgcagca tgtagtgatt
     6661 gggcccagta gcctgattgt gcatttcaat gaagtcatag gaagagggca ttttggttgt
     6721 gtatatcatg ggactttgtt ggacaatgat ggcaagaaaa ttcactgtgc tgtgaaatcc
     6781 ttgaacagaa tcactgacat aggagaagtt tcccaatttc tgaccgaggg aatcatcatg
     6841 aaagatttta gtcatcccaa tgtcctctcg ctcctgggaa tctgcctgcg aagtgaaggg
     6901 tctccgctgg tggtcctacc atacatgaaa catggagatc ttcgaaattt cattcgaaat
     6961 gagactcata atccaactgt aaaagatctt attggctttg gtcttcaagt agccaaaggc
     7021 atgaaatatc ttgcaagcaa aaagtttgtc cacagagact tggctgcaag aaactgtatg
     7081 ctggatgaaa aattcacagt caaggttgct gattttggtc ttgccagaga catgtatgat
     7141 aaagaatact atagtgtaca caacaaaaca ggtgcaaagc tgccagtgaa gtggatggct
     7201 ttggaaagtc tgcaaactca aaagtttacc accaagtcag atgtgtggtc ctttggcgtg
     7261 ctcctctggg agctgatgac aagaggagcc ccaccttatc ctgacgtaaa cacctttgat
     7321 ataactgttt acttgttgca agggagaaga ctcctacaac ccgaatactg cccagacccc
     7381 ttatatgaag taatgctaaa atgctggcac cctaaagccg aaatgcgccc atccttttct
     7441 gaactggtgt cccggatatc agcgatcttc tctactttca ttggggagca ctatgtccat
     7501 gtgaacgcta cttatgtgaa cgtaaaatgt gtcgctccgt atccttctct gttgtcatca
     7561 gaagataacg ctgatgatga ggtggacaca cgaccagcct ccttctggga gacatcatag
     7621 aacccagctt tcttgtacaa agtggtgata tccaattaac cctcactaaa ggatttatgt
     7681 agttgagagt gataaaccct atagtgagtc gtattaccac ccaacttttc tatacaaagt
     7741 ggttgatatc cagcacagtg gcggccgctc gagtctagag ggcccgcggt tcgaaggtaa
     7801 gcctatccct aaccctctcc tcggtctcga ttctacgcgt accggttagt aatgagatcc
     7861 ctcccccccc cctaacgtta ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt
     7921 ctatatgtta ttttccacca tattgccgtc ttttggcaat gtgagggccc ggaaacctgg
     7981 ccctgtcttc ttgacgagca ttcctagggg tctttcccct ctcgccaaag gaatgcaagg
     8041 tctgttgaat gtcgtgaagg aagcagttcc tctggaagct tcttgaagac aaacaacgtc
     8101 tgtagcgacc ctttgcaggc agcggaaccc cccacctggc gacaggtgcc tctgcggcca
     8161 aaagccacgt gtataagata cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag
     8221 ttggatagtt gtggaaagag tcaaatggct ctcctcaagc gtattcaaca aggggctgaa
     8281 ggatgcccag aaggtacccc attgtatggg atctgatctg gggcctcggt gcacatgctt
     8341 tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt
     8401 tttcctttga aaaacacgat gataatatgg ccacacatat gaccgagtac aagcccacgg
     8461 tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc gccgcgttcg
     8521 ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag cgggtcaccg
     8581 agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg
     8641 acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt
     8701 tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac
     8761 agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg
     8821 tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag
     8881 tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc
     8941 tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac
     9001 cgcgcacctg gtgcatgacc cgcaagcccg gtgcctaaat cgatagatcc taatcaacct
     9061 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     9121 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     9181 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     9241 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     9301 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     9361 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     9421 gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt
     9481 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     9541 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     9601 cctcagacga gtcggatctc cctttgggcc gcctccccgc ctgagatcct ttaagaccaa
     9661 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag
     9721 ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt
     9781 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     9841 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     9901 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat
     9961 gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag agtgagaggc
    10021 ccgggttaat taaggaaagg gctagatcat tcttgaagac gaaagggcct cgtgatacgc
    10081 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt
    10141 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
    10201 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
    10261 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
    10321 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
    10381 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
    10441 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
    10501 gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
    10561 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
    10621 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
    10681 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
    10741 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
    10801 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
    10861 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
    10921 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
    10981 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
    11041 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
    11101 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
    11161 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
    11221 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
    11281 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
    11341 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
    11401 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
    11461 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
    11521 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
    11581 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
    11641 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
    11701 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
    11761 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
    11821 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
    11881 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
    11941 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
    12001 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
    12061 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagcaagctc
    12121 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
    12181 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctccccgtgg
    12241 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
    12301 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
    12361 attgtgagcg gataacaatt tcacacagga aacagctatg acatgattac gaatttcaca
    12421 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
    12481 tatcatgtct ggatcaactg gataactcaa gctaaccaaa atcatcccaa acttcccacc
    12541 ccatacccta ttaccactgc caattacctg tggtttcatt tactctaaac ctgtgattcc
    12601 tctgaattat tttcatttta aagaaattgt atttgttaaa tatgtactac aaacttagta
    12661 gt
//
