LOCUS       sequence-241044-       12808 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 515, Emission: 528.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12808)
  TITLE     Davidson Yellow fluorescent proteins
REFERENCE   2  (bases 1 to 12808)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mVenus-Talin-18
FEATURES             Location/Qualifiers
     source          1..12808
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        199..502
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        503..706
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     657..677
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      745..754
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(751..753,754..756,757..1467)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(796..817)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1057..1076)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1404..1425
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     regulatory      1516..1525
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    9734..9855
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9771..9790)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9825..9844
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(9862..10317)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9999..10020)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     10211..10230
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        10344..10448
                     /gene="bla"
                     /label=AmpR promoter
     promoter        10450..10807
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        10450..10641
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(10473..10493)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      10658..10793
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             10842..11636
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             10842..11636
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(10896..10915)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     11506..11525
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(11824..11843)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    11868..11915
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      join(12244..12808,1..24)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12733..12752
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
       61 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
      121 cgtattaccg ccatgcatta gttattaata gtaatcaatt acggggtcat tagttcatag
      181 cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
      241 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
      301 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
      361 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
      421 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
      481 attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata
      541 gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt
      601 ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca
      661 aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctggtt tagtgaaccg
      721 tcagatccgc tagcgctacc ggtcgccacc atggtgagca agggcgagga gctgttcacc
      781 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg
      841 tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagct gatctgcacc
      901 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgggcta cggcctgcag
      961 tgcttcgccc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc
     1021 gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc
     1081 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac
     1141 ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac
     1201 gtctatatca ccgccgacaa gcagaagaac ggcatcaagg ccaacttcaa gatccgccac
     1261 aacatcgagg acggcggcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc
     1321 gacggccccg tgctgctgcc cgacaaccac tacctgagct accagtccaa actgagcaaa
     1381 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc
     1441 actctcggca tggacgagct gtacaagtcc ggactcagat ctcgagctca agctagcttc
     1501 gaattcgcag aagctgccac catggttgcg ctttcgctga agattagcat tgggaatgtg
     1561 gtgaagacga tgcaatttga gccatctacc atggtgtacg acgcctgccg catgattcgt
     1621 gagcggatcc cagaggccct ggctggccct cccaacgact ttgggctctt tctgtcagat
     1681 gatgacccca aaaaaggcat ctggctagag gctgggaaag ctttggacta ctacatgctc
     1741 cgaaatgggg acaccatgga gtacagaaaa aagcagagac ccctgaagat acggatgtta
     1801 gacggaacgg tgaagactat catggtggat gactccaaga ccgtcacaga catgctcatg
     1861 accatttgtg cccgaattgg tatcaccaac catgatgagt attcactggt tcgagagctg
     1921 atggaagaaa agaaagatga ggggacaggg accctgagaa aagacaagac cctactgcga
     1981 gatgaaaaga agatggagaa gctaaagcag aagttgcaca cagacgatga gttgaactgg
     2041 ctggaccatg ggcggacact gagggaacag ggagtggaag agcatgagac gttgctgctg
     2101 cggagaaagt tcttctactc agaccagaat gtggattccc gagaccctgt acagctgaac
     2161 cttctctatg tgcaggcacg agatgacatc ctgaatggct cccatcctgt ctcctttgac
     2221 aaggcctgtg aatttgcagg cttccagtgc cagatccagt ttggacctca caatgaacag
     2281 aagcacaagg ctggcttcct tgacctgaag gacttcctgc ccaaggagta tgtgaagcag
     2341 aagggagagc gtaagatctt tcaggcacac aagaattgtg ggcagatgag tgaaattgag
     2401 gccaaggtac gctacgtgaa gctggcccgt tccctcaaga cttacggtgt ctccttcttc
     2461 ctagtcaagg aaaagatgaa ggggaagaat aaactggtgc ccaggctgtt gggcatcact
     2521 aaggagtgtg tgatgcgtgt ggatgagaag accaaggagg tgattcagga atggagcctc
     2581 accaacatca aacgctgggc tgcctctccc aagagcttta ctctggactt tggagactat
     2641 caggatggct actactcagt acagacgaca gaaggcgagc agatcgcaca gctcattgct
     2701 ggctacatag atatcatcct taagaagaaa aaaagcaagg accattttgg gctggaggga
     2761 gatgaagagt ctactatgtt ggaggactca gtgtctccca aaaagtcaac agtccttcag
     2821 cagcagtaca accgagtggg gaaagtggag cacggctctg tggctctgcc agccatcatg
     2881 cgctctggag cctctggtcc tgagaatttc caggtgggga gcatgccacc tgcccagcag
     2941 cagatcacca gtggccagat gcaccgagga cacatgccac ctctgacttc agcccagcag
     3001 gcgctcactg gaaccattaa ctccagcatg caggctgtgc aggctgccca ggccactctg
     3061 gatgactttg agactctacc ccctcttggc caggatgctg cctccaaagc ttggcgtaag
     3121 aacaagatgg atgaatcaaa gcatgagatc cactcccagg tagatgccat cacagctggc
     3181 actgcctctg tggtaaacct gacagcagga gatcctgcag agacagacta tacagcagtg
     3241 ggctgtgcag tcaccaccat ctcttccaac ctgacggaga tgtcccgtgg ggtgaagcta
     3301 ttggctgccc tgctggagga tgaaggcggc aatggccggc cccttctgca agcagcaaag
     3361 ggccttgcag gggctgtgtc ggaactgctt cgcagtgctc agcccgccag cgctgagcct
     3421 cgtcagaacc tgctgcaggc agccgggaac gtgggccagg ccagtgggga gctgttgcag
     3481 caaattgggg aaagtgacac tgacccccac ttccaggatg ttctaatgca gctagcaaac
     3541 gcagtggcaa gtgctgcagc cgccctggtc ctcaaggcca agagtgtggc ccagcgcaca
     3601 gaggattccg ggcttcagac ccaagtgatt gctgcagcta cgcaatgtgc tctgtccact
     3661 tcccagctgg tggcctgtac caaggtggta gcacctacaa tcagctcacc tgtgtgccaa
     3721 gaacagctag tcgaggctgg gcgactggtg gccaaagctg tggagggctg tgtgtctgcc
     3781 tcgcaagcag ctacagaaga tggacagctg cttcgagggg taggagcagc agccacggct
     3841 gtcacccagg ccctcaatga gctgctgcag cacgtgaagg cccacgccac cggagctggg
     3901 cctgccggtc ggtatgacca agccactgat accatcctca ccgtcactga aaacatcttc
     3961 agctccatgg gtgatgctgg ggagatggtg cgccaggccc gcatcctggc ccaagccacc
     4021 tcagacctgg tcaatgctat caaggccgat gctgaggggg agagtgatct ggagaactct
     4081 agaaagctcc tgagtgctgc caagatcctc gctgatgcca ccgccaagat ggtggaggcg
     4141 gccaagggag cagccgccca ccctgacagt gaggaacagc agcagcgact gcgtgaagca
     4201 gctgaggggc ttcgcatggc caccaatgca gctgcgcaga acgccatcaa gaagaagttg
     4261 gtgcagcgcc tggagcatgc agccaagcaa gctgcagcct ctgcaacaca gaccattgct
     4321 gcagcccaac atgcagcctc tgcccccaag gcctctgcgg gcccccagcc cctactggtg
     4381 cagagctgta aggccgtggc agagcagatt ccgttgctgg tgcagggtgt ccgaggaagt
     4441 caagctcagc ctgacagccc tagtgctcag ctcgccctca ttgctgccag ccagagcttc
     4501 ctgcagccag gtgggaagat ggtggcagcg gcaaaagcct cagtgccaac aattcaggac
     4561 caggcttcag cgatgcagct gagtcagtgc gcaaagaacc taggcactgc cctggccgag
     4621 ctgcgcactg ctgctcagaa ggctcaggag gcatgtggac ctttggagat ggattctgca
     4681 ctgagtgttg tacaaaatct agagaaagac ctacaggaaa taaaagcagc agctcgagat
     4741 ggcaagctta agcccttacc tggggaaaca atggagaagt gtacccaaga tcttggcaac
     4801 agcaccaaag cggtgagctc tgccatcgcc aagctgctgg gggagattgc ccagggcaac
     4861 gagaactatg caggtattgc agctcgggat gtagctggtg gactaaggtc actagcccag
     4921 gctgcgcggg gtgtggctgc gctgacatca gatcctgcag tgcaggccat tgtgcttgac
     4981 acagccagcg acgttctgga caaggccagc agcctcattg aagaggcaaa aaaagcatct
     5041 ggacacccag gggacccaga aagccagcaa aggcttgctc aggtagctaa agcagtgacc
     5101 caagccctga accgctgtgt cagctgcctg cctggccaaa gagacgtgga taatgcccta
     5161 cgagccgttg gagatgccag caagcgactc ctgagtgact tgcttcctcc aagcacgggg
     5221 acatttcaag aagcacagag ccgattgaat gaagctgcag ctgggttaaa tcaggccgcc
     5281 acagaactgg tgcaagcctc ccgaggaact cctcaggacc tggctcgggc ctccggccga
     5341 tttggacagg acttcagcac cttcctggaa gctggtgtgg agatggccgg acaggccccg
     5401 agccaggagg accgagccca ggtggtgtcc aacctgaagg gcatatccat gtcttcaagc
     5461 aaacttctcc ttgccgctaa ggccttgtcc acagaccctg cttctcccaa cctcaagagt
     5521 cagctggctg cggctgcccg ggcagtgacg gacagcatca accagctcat caccatgtgc
     5581 acccagcagg cacctggcca gaaggagtgt gacaatgcac ttcggcagct agagacagtc
     5641 cgagaactcc tggagaatcc agtccagccc atcaacgaca tgtcctactt cggttgcttg
     5701 gacagtgtca tggagaactc caaggtccta ggtgaggcca tgactggcat ctcccaaaat
     5761 gccaagaatg gaaatctgcc ggagtttgga gatgccattg ccacagcctc caaggctctc
     5821 tgtggcttca cggaggcagc cgcacaggca gcatatctag ttggtgtctc tgaccccaac
     5881 agccaagctg gacagcaagg actggtggaa cccacacagt ttgcccgtgc aaaccaggca
     5941 attcagatgg cctgtcagag tctgggggag cctggctgta cccaggccca ggtgttatct
     6001 gcagccacta ttgtagccaa acacacatct gcattgtgta acagctgtcg cctggcttcc
     6061 gctaggactg ccaatcccac tgccaagcgc cagtttgtac agtcagccaa ggaggtggcc
     6121 aacagtacag ccaatcttgt caagaccatc aaggcactag atggggactt cacagaagag
     6181 aaccgtgccc agtgccgagc agccacagcc cctctgctgg aagctgtgga taacctgagt
     6241 gcctttgcct ccaaccctga gttctccagc gtccctgccc agatcagccc tgagggcaga
     6301 gcagccatgg agcccattgt aatctctgct aagacaatgt tggagagtgc tggagggctc
     6361 atccagacag ctcgggcgtt agcagtcaat ccccgagacc ccccacgttg gtctgtgcta
     6421 gctggccact ctcggactgt ctcagattcc atcaagaaac ttattacaag catgagggac
     6481 aaagccccag ggcagctgga gtgtgagaca gccattgcgg ctctgaacag ctgcttgcgg
     6541 gacctagatc aggcttcgct tgccgctgtc agccagcagc ttgctccccg tgaaggaatc
     6601 tctcaagagg ctttgcacac ccagatgctc actgcagtgc aagaaatttc tcatctcatt
     6661 gagccgctgg ccagcgctgc tcgggctgaa gcctcccagc tgggacacaa ggtgtcccaa
     6721 atggcccagt actttgaacc actcaccctg gctgcagtgg gtgctgcgtc taagaccctg
     6781 agccacccgc agcagatggc acttctggac cagactaaaa cgttggcaga gtctgccttg
     6841 cagttgctat atactgccaa ggaggctggt ggtaacccca agcaagcagc acacacccag
     6901 gaagccctgg aggaggctgt gcagatgatg acagaggccg tagaagacct gacgacgacc
     6961 ctcaacgaag cagccagtgc tgcaggagtc gttggcggca tggtggactc tatcactcag
     7021 gccatcaacc agctagatga aggacctatg ggtgacccag aaggctcatt cgtagattac
     7081 cagacaacca tggtgaggac agccaaggcc attgctgtca ctgttcagga gatggtaacc
     7141 aagtcaaaca ccagccctga agagctaggc cctcttgcca accagctgac cagtgactat
     7201 ggccgactgg cctcacaagc caagcctgca gctgtggctg ctgaaaatga agagataggc
     7261 gctcacatca aacaccgagt acaggagctg ggccatggct gctctgctct ggtcaccaag
     7321 gcaggtgcct tgcagtgtag ccccagtgat gtctacacca agaaggagct catagagtgt
     7381 gcccgcagag tgtcagagaa ggtctcccat gttctggctg cactccaggc tgggaatcgt
     7441 ggtacccagg cctgcattac agcagccagt gctgtgtctg gtatcattgc tgacctcgac
     7501 accaccatca tgttcgctac tgctggcaca cttaaccgtg agggtgctga aacttttgct
     7561 gaccaccggg agggtatctt aaagacagca aaggttcttg tggaggacac caaggtccta
     7621 gtgcagaatg cagctgggag ccaggagaag ttggcacaag ccgcccagtc ctccgtggcc
     7681 accattaccc gcctcgctga tgtggtcaag ctcggtgcag ccagcctagg agccgaagac
     7741 cctgaaactc aggtggtgct gatcaatgca gtaaaggacg tagccaaggc cctgggtgac
     7801 ctcatcagcg ctacgaaggc tgcagcgggc aaagttgggg atgaccctgc agtgtggcag
     7861 ctcaagaact ctgccaaggt gatggtgacc aatgtgacat cattgctcaa gacagtgaag
     7921 gctgtggaag atgaggccac caaaggcaca cgggccctag aggcaaccac agagcacata
     7981 cgtcaggaac tggcggtctt ctgttcccca gagccacctg ccaagacctc tacccctgaa
     8041 gacttcatcc gaatgaccaa gggtattact atggcaacag ccaaagccgt tgctgctggc
     8101 aattcctgtc gacaggaaga tgtcattgcc acagccaatc tgagccgacg ggctattgcg
     8161 gacatgcttc gggcttgcaa ggaagcagct ttccacccag aagtggcgcc tgatgtacgg
     8221 ctccgagccc tgcactatgg ccgggagtgt gccaatggtt acctggaact gctggaccac
     8281 gtgctgctga cccttcagaa gccaaaccca gacctgaagc agcagctaac tggacactca
     8341 aagcgggttg ctggctctgt gactgagctc atccaggctg ctgaagccat gaaaggaaca
     8401 gagtgggtgg acccagagga ccctactgtc attgctgaga atgagctctt gggagccgca
     8461 gccgccatcg aggctgcagc caagaagctg gagcagctga agcccagggc caaacccaag
     8521 gaggcggatg agtccttgaa ctttgaggaa caaatcctag aagctgccaa gtccatcgct
     8581 gcagccacca gtgcactggt aaaggctgcg tcagcagccc agagggagct ggtggctcaa
     8641 ggaaaggtgg gcgccattcc agccaatgca ctggatgatg ggcagtggtc gcagggcctc
     8701 atttccgctg cccgtatggt ggctgcggcc accaacaatc tgtgtgaagc agctaacgca
     8761 gctgtccagg gccatgctag ccaggagaaa ctcatttcct cagccaagca agtagctgcc
     8821 tccacagctc agctcctggt agcttgcaag gtcaaagcag atcaggactc tgaggcaatg
     8881 aaacggctcc aggctgctgg caatgcagtg aagagggcct cagataacct ggtaaaggcg
     8941 gcccagaaag ctgcagcctt cgaagaccag gagaatgaga cggtggtggt gaaggagaaa
     9001 atggttgggg gcattgccca gattatcgca gcacaggaag agatgcttcg gaaggaacga
     9061 gagctggaag aggctcggaa aaagctcgcc cagatccggc agcagcagta caagttcttg
     9121 ccttcagagc ttcgagacga gcactaaaaa agccctgtgt atttaatgca gacccagccc
     9181 agagactgtg cctgccacta ccaaagcctt ctgggctgct caggacccaa gctgcccaac
     9241 cccagcactc ccccaaagtg cctgccaaac cctgggcctg gccctgccca gtcccactgc
     9301 aagccgtgtc ctctccctga ctcccaagtg cctttgcacc ctagggcccc taagtgcctg
     9361 cccccctcca gagtattaac gctccaagag tattattaat gctgccgtac ctcagtttga
     9421 acctgccagg gccccagctg ctccagcctg ccagcagctt ccagccagtc cccacggcca
     9481 cgtcagctca actcatccct ttttgatact atatccccta cccagctacc tatggggctt
     9541 gagggttgta aacccaaaca ggtcagactc ccggaattct gcagtcgacg gtaccgcggg
     9601 cccgggatcc accggatcta gataactgat cataatcagc cataccacat ttgtagaggt
     9661 tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc
     9721 aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     9781 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     9841 catcaatgta tcttaacgcg taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa
     9901 atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata
     9961 aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac
    10021 tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc
    10081 cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa
    10141 atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg
    10201 cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg
    10261 tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtcag
    10321 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
    10381 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
    10441 ggaagagtcc tgaggcggaa agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa
    10501 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
    10561 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
    10621 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
    10681 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
    10741 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
    10801 ttgcaaagat cgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat
    10861 tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac
    10921 agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc
    10981 tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaagacgag gcagcgcggc
    11041 tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag
    11101 cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc
    11161 ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg
    11221 atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc
    11281 ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc
    11341 cagccgaact gttcgccagg ctcaaggcga gcatgcccga cggcgaggat ctcgtcgtga
    11401 cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca
    11461 tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg
    11521 atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg
    11581 ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg
    11641 gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga
    11701 ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg
    11761 gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccta gggggaggct
    11821 aactgaaaca cggaaggaga caataccgga aggaacccgc gctatgacgg caataaaaag
    11881 acagaataaa acgcacggtg ttgggtcgtt tgttcataaa cgcggggttc ggtcccaggg
    11941 ctggcactct gtcgataccc caccgagacc ccattggggc caatacgccc gcgtttcttc
    12001 cttttcccca ccccaccccc caagttcggg tgaaggccca gggctcgcag ccaacgtcgg
    12061 ggcggcaggc cctgccatag cctcaggtta ctcatatata ctttagattg atttaaaact
    12121 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
    12181 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
    12241 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
    12301 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
    12361 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
    12421 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
    12481 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
    12541 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
    12601 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
    12661 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
    12721 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
    12781 acttgagcgt cgatttttgt gatgctcg
//
