LOCUS       sequence-241053-       11722 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express 3xNLS-Klf4 TALE-GCN4 engineered to bind a site in the human 
            KLF4 gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11722)
  AUTHORS   Zhao C, Zhang Y, Zhao Y, Ying Y, Ai R, Zhang J, Wang Y
  TITLE     Multiple Chemical Inducible Tal Effectors for Genome Editing and 
            Transcription Activation.
  JOURNAL   ACS Chem Biol. 2018 Mar 16;13(3):609-617. doi: 
            10.1021/acschembio.7b00606. Epub 2018 Jan 8.
  PUBMED    29308880
REFERENCE   2  (bases 1 to 11722)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acschembio.7b00606"; journalName: "ACS Chem Biol"; date: 
            "2018-03-16- 16"; volume: "13"; issue: "3"; pages: "609-617"
COMMENT     Sequence Label: EF1a-3NLS-Klf4 TALE-GCN4
FEATURES             Location/Qualifiers
     source          1..11722
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        129..340
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      1204..1213
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1225..1245
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1249..1269
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1273..1293
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3913..3969
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             3985..4041
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4057..4113
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4129..4185
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4201..4257
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4273..4329
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4345..4401
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4417..4473
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4489..4545
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4561..4617
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     primer_bind     complement(4841..4861)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4846..5175
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5026..5161
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5185..5559
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     misc_feature    5645..6233
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5698..5718)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6116..6127)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6320..6553
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6625..6746
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      6765..6900
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6920..6939)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6921..6939)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6949..6966)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6949..6965)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6958..6980)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7107..7562
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7194..7213)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7404..7425
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7588..7692
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7693..7761,7762..8553)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7911..7930)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8724..9312
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9213..9232
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9466..9483
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9600..9621
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9636..9641,9642..9659,9660..9666)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9674..9690
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9679..9701
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9698..9714
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9698..9714
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9733..9753
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9735..9753
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        9781..10007
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             10008..10188
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    10235..10360
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10853..11086
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11271..11315
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    11582..11699
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 tagcccgggc tcgaccatat gataagcttt gcaaagatgg ataaagtttt aaacagagag
       61 gaatctttgc agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc
      121 ccgtcagtgg gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg
      181 caattgaacc ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta
      241 ctggctccgc ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt
      301 gaacgttctt tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc
      361 ccgcgggcct ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg
      421 gctgcagtac gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag
      481 gccttgcgct taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg
      541 gggccgccgc gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc
      601 tctagccatt taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct
      661 tgtaaatgcg ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga
      721 cggggcccgt gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc
      781 gagaatcgga cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc
      841 gccgtgtatc gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc
      901 ggaaagatgg ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc
      961 gggagagcgg gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt
     1021 cgcttcatgt gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag
     1081 cttttggagt acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca
     1141 cactgagtgg gtggagactg aagttaggcc agcttggcac ttgatgtaat tctcctttct
     1201 agagccacca tgggtggcgc gcctccaaaa aagaagagaa aggtagatcc aaaaaagaag
     1261 agaaaggtag atccaaaaaa gaagagaaag gtaggattaa ttaatggaag tggaagtgga
     1321 agtggaggta ccatgtcgcg gacccggctc ccttccccac ccgcacccag cccagcgttt
     1381 tcggccgact cgttctcaga cctgcttagg cagttcgacc cctcactgtt taacacatcg
     1441 ttgttcgact cccttcctcc gtttggggcg caccatacgg aggcggccac cggggagtgg
     1501 gatgaggtgc agtcgggatt gagagctgcg gatgcaccac ccccaaccat gcgggtggcc
     1561 gtcaccgctg cccgaccgcc gagggcgaag cccgcaccaa ggcggagggc agcgcaaccg
     1621 tccgacgcaa gccccgcagc gcaagtagat ttgagaactt tgggatattc acagcagcag
     1681 caggaaaaga tcaagcccaa agtgaggtcg acagtcgcgc agcatcacga agcgctggtg
     1741 ggtcatgggt ttacacatgc ccacatcgta gccttgtcgc agcaccctgc agcccttggc
     1801 acggtcgccg tcaagtacca ggacatgatt gcggcgttgc cggaagccac acatgaggcg
     1861 atcgtcggtg tggggaaaca gtggagcgga gcccgagcgc ttgaggccct gttgacggtc
     1921 gcgggagagc tgagagggcc tccccttcag ctggacacgg gccagttgct gaagatcgcg
     1981 aagcggggag gagtcacggc ggtcgaggcg gtgcacgcgt ggcgcaatgc gctcacggga
     2041 gcacccctca acctgacccc agagcaggtc gtggccattg cctcgaatgg agggggcaaa
     2101 caggcgttgg aaaccgtaca acgattgctg ccggtgctgt gccaagcgca cggactcacc
     2161 ccagagcagg tcgtggcgat cgcaagccac gacggaggaa agcaagcctt ggaaacagta
     2221 cagaggctgt tgcctgtgct gtgccaagcg cacggcctca ccccagagca ggtcgtggcc
     2281 attgcctcga atggaggggg caaacaggcg ttggaaaccg tacaacgatt gctgccggtg
     2341 ctgtgccaag cgcacggatt aaccccagag caggtcgtgg ccattgcctc gaatggaggg
     2401 ggcaaacagg cgttggaaac cgtacaacga ttgctgccgg tgctgtgcca agcgcacggc
     2461 ttaaccccag agcaggtcgt ggcaatcgcc tccaacattg gcgggaaaca ggcactcgag
     2521 actgtccagc gcctgcttcc cgtgctgtgc caagcgcacg gacttacccc agagcaggtc
     2581 gtggcgatcg caagccacga cggaggaaag caagccttgg aaacagtaca gaggctgttg
     2641 cctgtgctgt gccaagcgca cggccttacc ccagagcagg tcgtggccat tgcctcgaat
     2701 ggagggggca aacaggcgtt ggaaaccgta caacgattgc tgccggtgct gtgccaagcg
     2761 cacggactaa ccccagagca ggtcgtggcc attgcctcga atggaggggg caaacaggcg
     2821 ttggaaaccg tacaacgatt gctgccggtg ctgtgccaag cgcacgggct caccccagag
     2881 caggtcgtgg caatcgcctc caacattggc gggaaacagg cactcgagac tgtccagcgc
     2941 ctgcttcccg tgctgtgcca agcgcacggg ctaaccccag agcaggtcgt ggccattgcc
     3001 tcgaatggag ggggcaaaca ggcgttggaa accgtacaac gattgctgcc ggtgctgtgc
     3061 caagcgcacg gcctaacccc agagcaggtc gtggcaatcg cctccaacat tggcgggaaa
     3121 caggcactcg agactgtcca gcgcctgctt cccgtgctgt gccaagcgca cgggttaacc
     3181 ccagagcagg tcgtggcaat cgcctccaac attggcggga aacaggcact cgagactgtc
     3241 cagcgcctgc ttcccgtgct gtgccaagcg cacggactca cgcctgagca ggtagtggct
     3301 attgcatccc atgacggggg cagacccgca ctggagtcaa tcgtggccca gctttcgagg
     3361 ccggaccccg cgctggccgc actcactaat gatcatcttg tagcgctggc ctgcctcggc
     3421 ggacgacccg ccttggatgc ggtgaagaag gggctcccgc acgcgcctgc attgattaag
     3481 cggaccaaca gaaggattcc cgagaggaca tcacatcgag tggcagatca cgcgcaagtg
     3541 gtccgcgtgc tcggattctt ccagtgtcac tcccaccccg cacaagcgtt cgatgacgcc
     3601 atgactcaat ttggtatgtc gagacacgga ctgctgcagc tctttcgtag agtcggtgtc
     3661 acagaactcg aggcccgctc gggcacactg cctcccgcct cccagcggtg ggacaggatt
     3721 ctccaagcga gcggtatgaa acgcgcgaag ccttcaccta cgtcaactca gacacctgac
     3781 caggcgagcc ttcatgcgtt cgcagactcg ctggagaggg atttggacgc gccctcgccc
     3841 atgcatgaag gggaccaaac tcgcgcgtca gctggatccg ccatcataca cgcggccgca
     3901 acaagcggtc cggaagaact tttgagcaag aattatcatc ttgagaacga agtggctcgt
     3961 cttaagaaag gttctggcag tggagaagaa ctgctttcaa agaattacca cctggaaaat
     4021 gaggtagcta gactgaaaaa ggggagcgga agtggggagg agttgctgag caaaaattat
     4081 catttggaga acgaagtagc acgactaaag aaagggtccg gatcgggtga ggagttactc
     4141 tcgaaaaatt atcatctcga aaacgaagtg gctcggctaa aaaagggcag tggttctgga
     4201 gaagagctat tatctaaaaa ctaccacctc gaaaatgagg tggcacgctt aaaaaaggga
     4261 agtggcagtg gtgaagagct actatccaag aattatcatc ttgagaacga ggtagcgcgt
     4321 ttgaagaagg gttccggctc aggagaggaa ctgctctcga agaactatca tcttgaaaat
     4381 gaggtcgctc gattaaaaaa gggatcgggc agtggtgagg aactactttc aaagaattac
     4441 cacctcgaaa acgaagtagc tcgattaaag aaaggttcag ggtcgggtga agaattactg
     4501 agtaaaaatt atcatctgga aaatgaggta gcgagactaa aaaaggggag tggttctggc
     4561 gaggaattgc tatcgaaaaa ttatcatctt gagaacgaag ttgctaggct caaaaagggc
     4621 tcaggctcag gctgagttta aacatcgcct gcaggggaag tggaagtgat atcggaagtg
     4681 gaagcactag tggaagtttc gaagtcgact tgtttattgc agcttataat ggttacaaat
     4741 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     4801 gtttgtccaa actcatcaat gtatcttatg tcgaccctgt ggaatgtgtg tcagttaggg
     4861 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     4921 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     4981 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
     5041 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     5101 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     5161 ctaggctttt gcaaaaagct taccatggcc aagttgacca gtgccgttcc ggtgctcacc
     5221 gcgcgcgacg tcgccggagc ggtcgagttc tggaccgacc ggctcgggtt ctcccgggac
     5281 ttcgtggagg acgacttcgc cggtgtggtc cgggacgacg tgaccctgtt catcagcgcg
     5341 gtccaggacc aggtggtgcc ggacaacacc ctggcctggg tgtgggtgcg cggcctggac
     5401 gagctgtacg ccgagtggtc ggaggtcgtg tccacgaact tccgggacgc ctccgggccg
     5461 gccatgaccg agatcggcga gcagccgtgg gggcgggagt tcgccctgcg cgacccggcc
     5521 ggcaactgcg tgcacttcgt ggccgaggag caggactgac cgatcgataa aataaaagat
     5581 tttatttagt ctccagaaaa aggggggaat gaaagacccc acctgtaggt ttggcaagct
     5641 cgacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
     5701 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
     5761 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga
     5821 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc
     5881 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct
     5941 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
     6001 gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct
     6061 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc
     6121 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg
     6181 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctggaat
     6241 tcgagctcag tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt
     6301 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt
     6361 tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     6421 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     6481 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     6541 aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag
     6601 aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa
     6661 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     6721 ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc
     6781 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     6841 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     6901 tagggacgta cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt
     6961 tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca
     7021 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
     7081 gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg
     7141 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     7201 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     7261 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     7321 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     7381 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     7441 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     7501 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat
     7561 ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     7621 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     7681 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     7741 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     7801 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     7861 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     7921 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     7981 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     8041 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     8101 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     8161 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     8221 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     8281 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     8341 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     8401 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     8461 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     8521 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     8581 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     8641 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     8701 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     8761 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     8821 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     8881 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     8941 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     9001 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     9061 cagcccagct tggagcgaac gacctacacc aaactgagat acctacagcg tgagctatga
     9121 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     9181 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     9241 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     9301 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     9361 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     9421 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     9481 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
     9541 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
     9601 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt
     9661 atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat
     9721 tacgccaagc gcgcaattaa ccctcactaa agggaacaaa agctggagct gcaagcttaa
     9781 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
     9841 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc
     9901 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc
     9961 cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt
    10021 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
    10081 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
    10141 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
    10201 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
    10261 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
    10321 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
    10381 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
    10441 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
    10501 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
    10561 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
    10621 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
    10681 gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca
    10741 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
    10801 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
    10861 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
    10921 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
    10981 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
    11041 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
    11101 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
    11161 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
    11221 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
    11281 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
    11341 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
    11401 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
    11461 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg
    11521 gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcggttaa
    11581 cttttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa
    11641 tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaatttta
    11701 tcgatcacga gactagcctc ga
//
