LOCUS       sequence-24107-e        1107 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1107)
  TITLE     T4 Lysozyme Plasmids - Tray 2
REFERENCE   2  (bases 1 to 1107)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: T4 Lysozyme S44-[AAA] L46A WT*
FEATURES             Location/Qualifiers
     source          1..1107
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    732..748
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(756..762,763..778,779..784))
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    827..843
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(851..857,858..873,874..879))
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
ORIGIN
        1 ctangaaaaa gcccgctcat taggcgggct cagatctgct catgtttgac agcttatcat
       61 cgataagctt cggcaataaa ttcttgatat gttttcatat gtttccttta aatattttaa
      121 ttagtaattg tctattcaag taattgtgaa tatactatca caattccaag agaaagtaaa
      181 cagctttata gatttttata cgcgtcccaa gtgccagttc taaacgttgt aatgactcgt
      241 tttgcgcgat taggtgtttg attataccat ctacttttag ctaagttaac tgctgcttca
      301 tcccagcgtt tttgttgaag catacgtaaa gagttagtaa atcctgccac accggtttct
      361 cccatttgga aaaccatatt aatcaatgca gcgcgacgaa ccgcatcaag agaatcataa
      421 accggtttta atttagcatt tctcagaatt ccgcgaacag cagcatcaac atcctgatta
      481 aagagttttt cagcctcatc ttttgtaatt acaccattag tattacgccc aatagcttta
      541 tctgcttctg cagctgcaga tttagcagca ttaagtgatg gactttttgt aagcaaatga
      601 ccgatgccaa tagtgtaata gccttctgtg tctttataga ttttaagtct aagaccttca
      661 tctatacgta acatttcaaa tatattcata atacctccta agcatcgatg gatcctgttt
      721 cctgtgtgaa attgttatcc gctcacaatt ccacacatta tacgagccga tgattaattg
      781 tcaacagggg gatggggagt aagctgatcc tgtttcctgt gtgaaattgt tatccgctca
      841 caattccaca cattatacga gccgatgatt aattgtcaac agggggatgg ggagtaagct
      901 catcgatgga tcgaccgggn ttnccggggg gaaatggtta tccgccccca attccncaca
      961 ttanacgagc cggaagcnta aagtgtaaag cctggggggc ctaangagng agctaactta
     1021 cnntaantgg gttgcgctca cngnccgctt tccagncggg aaaccggncg ngccaggann
     1081 ccnncnaang gggnaaaacn ttnnngg
//
