LOCUS       sequence-241075-        5826 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5826)
  AUTHORS   Wrana JL, Attisano L, Carcamo J, Zentella A, Doody J, Laiho M, Wang 
            XF, Massague J
  TITLE     TGF beta signals through a heteromeric protein kinase receptor 
            complex.
  JOURNAL   Cell. 1992 Dec 11. 71(6):1003-14.
  PUBMED    1333888
REFERENCE   2  (bases 1 to 5826)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 1992 
            Dec 11. 71(6):1003-14."
COMMENT     Sequence Label: pARE-lux
FEATURES             Location/Qualifiers
     source          1..5826
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     133..152
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(371..475)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      502..957
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(589..608)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     799..820
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    1147..1268
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             1597..3249
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     complement(1663..1681)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     intron          3490..3555
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             3685..3705
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    4130..4264
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4406..4423)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4577..5165)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4657..4676)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5336..5826,1..301,302..370))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
       61 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
      121 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
      181 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
      241 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
      301 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
      361 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
      421 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
      481 ttccccgaaa agtgccacct gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
      541 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
      601 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
      661 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
      721 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
      781 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
      841 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
      901 agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttgcc
      961 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
     1021 tacgccagcc caagctacca tgataagtaa gtaatattaa ggtacgtgga ggttttactt
     1081 gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt
     1141 gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     1201 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     1261 gtatcttatg gtactgtaac tgagctaaca taacccggga ggtaccgagc tcttacgcgt
     1321 gctagctcga gcagctgaag cttgcatgcc tgcaggtcga ctctagtatc tgctgcccta
     1381 aaatgtgtat tccatggaaa tgtctgccct tctctcccta gtattgctgc cctaaaatgt
     1441 gtattccatg gaaatgtctg cccttctctc cctagtatct gctgccctaa aatgtgtatt
     1501 ccatggaaat gtctgccctt ctctccctag agggtatata atggatcccc gggtaccgag
     1561 ctcgaattag cttggcattc cggtactgtt ggtaaaatgg aagacgccaa aaacataaag
     1621 aaaggcccgg cgccattcta tcctctagag gatggaaccg ctggagagca actgcataag
     1681 gctatgaaga gatacgccct ggttcctgga acaattgctt ttacagatgc acatatcgag
     1741 gtgaacatca cgtacgcgga atacttcgaa atgtccgttc ggttggcaga agctatgaaa
     1801 cgatatgggc tgaatacaaa tcacagaatc gtcgtatgca gtgaaaactc tcttcaattc
     1861 tttatgccgg tgttgggcgc gttatttatc ggagttgcag ttgcgcccgc gaacgacatt
     1921 tataatgaac gtgaattgct caacagtatg aacatttcgc agcctaccgt agtgtttgtt
     1981 tccaaaaagg ggttgcaaaa aattttgaac gtgcaaaaaa aattaccaat aatccagaaa
     2041 attattatca tggattctaa aacggattac cagggatttc agtcgatgta cacgttcgtc
     2101 acatctcatc tacctcccgg ttttaatgaa tacgattttg taccagagtc ctttgatcgt
     2161 gacaaaacaa ttgcactgat aatgaattcc tctggatcta ctgggttacc taagggtgtg
     2221 gcccttccgc atagaactgc ctgcgtcaga ttctcgcatg ccagagatcc tatttttggc
     2281 aatcaaatca ttccggatac tgcgatttta agtgttgttc cattccatca cggttttgga
     2341 atgtttacta cactcggata tttgatatgt ggatttcgag tcgtcttaat gtatagattt
     2401 gaagaagagc tgtttttacg atcccttcag gattacaaaa ttcaaagtgc gttgctagta
     2461 ccaaccctat tttcattctt cgccaaaagc actctgattg acaaatacga tttatctaat
     2521 ttacacgaaa ttgcttctgg gggcgcacct ctttcgaaag aagtcgggga agcggttgca
     2581 aaacgcttcc atcttccagg gatacgacaa ggatatgggc tcactgagac tacatcagct
     2641 attctgatta cacccgaggg ggatgataaa ccgggcgcgg tcggtaaagt tgttccattt
     2701 tttgaagcga aggttgtgga tctggatacc gggaaaacgc tgggcgttaa tcagagaggc
     2761 gaattatgtg tcagaggacc tatgattatg tccggttatg taaacaatcc ggaagcgacc
     2821 aacgccttga ttgacaagga tggatggcta cattctggag acatagctta ctgggacgaa
     2881 gacgaacact tcttcatagt tgaccgcttg aagtctttaa ttaaatacaa aggatatcag
     2941 gtggcccccg ctgaattgga atcgatattg ttacaacacc ccaacatctt cgacgcgggc
     3001 gtggcaggtc ttcccgacga tgacgccggt gaacttcccg ccgccgttgt tgttttggag
     3061 cacggaaaga cgatgacgga aaaagagatc gtggattacg tcgccagtca agtaacaacc
     3121 gcgaaaaagt tgcgcggagg agttgtgttt gtggacgaag taccgaaagg tcttaccgga
     3181 aaactcgacg caagaaaaat cagagagatc ctcataaagg ccaagaaggg cggaaagtcc
     3241 aaattgtaaa atgtaactgt attcagcgat gacgaaattc ttagctattg taatactgcg
     3301 atgagtggca gggcggggcg taattttttt aaggcagtta ttggtgccct taaacgcctg
     3361 gttgctacgc ctgaataagt gataataagc ggatgaatgg cagaaattcg ccggatcttt
     3421 gtgaaggaac cttacttctg tggtgtgaca taattggaca aactacctac agagatttaa
     3481 agctctaagg taaatataaa atttttaagt gtataatgtg ttaaactact gattctaatt
     3541 gtttgtgtat tttagattcc aacctatgga actgatgaat gggagcagtg gtggaatgcc
     3601 tttaatgagg aaaacctgtt ttgctcagaa gaaatgccat ctagtgatga tgaggctact
     3661 gctgactctc aacattctac tcctccaaaa aagaagagaa aggtagaaga ccccaaggac
     3721 tttccttcag aattgctaag ttttttgagt catgctgtgt ttagtaatag aactcttgct
     3781 tgctttgcta tttacaccac aaaggaaaaa gctgcactgc tatacaagaa aattatggaa
     3841 aaatattctg taacctttat aagtaggcat aacagttata atcataacat actgtttttt
     3901 cttactccac acaggcatag agtgtctgct attaataact atgctcaaaa attgtgtacc
     3961 tttagctttt taatttgtaa aggggttaat aaggaatatt tgatgtatag tgccttgact
     4021 agagatcata atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc
     4081 acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat
     4141 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     4201 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     4261 gatccgtcga ccgatgccct tgagagcctt caacccagtc agctccttcc ggtgggcgcg
     4321 gggcatgact atcgtcgccg cacttatgac tgtcttcttt atcatgcaac tcgtaggaca
     4381 ggtgccggca gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     4441 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     4501 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     4561 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     4621 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     4681 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     4741 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     4801 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     4861 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     4921 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     4981 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     5041 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     5101 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     5161 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     5221 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     5281 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     5341 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     5401 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     5461 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     5521 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     5581 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     5641 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     5701 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     5761 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     5821 ttatgg
//
