LOCUS       sequence-241077-       11749 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express 3xNLS-Sox2 TALE-GCN4 engineered to bind a site in the human 
            SOX2 gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11749)
  AUTHORS   Zhao C, Zhang Y, Zhao Y, Ying Y, Ai R, Zhang J, Wang Y
  TITLE     Multiple Chemical Inducible Tal Effectors for Genome Editing and 
            Transcription Activation.
  JOURNAL   ACS Chem Biol. 2018 Mar 16;13(3):609-617. doi: 
            10.1021/acschembio.7b00606. Epub 2018 Jan 8.
  PUBMED    29308880
REFERENCE   2  (bases 1 to 11749)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acschembio.7b00606"; journalName: "ACS Chem Biol"; date: 
            "2018-03-16- 16"; volume: "13"; issue: "3"; pages: "609-617"
COMMENT     Sequence Label: EF1a-3NLS-Sox2TALE-GCN4
FEATURES             Location/Qualifiers
     source          1..11749
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        147..358
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      1222..1231
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1243..1263
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1267..1287
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1291..1311
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3956..4012
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4028..4084
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4100..4156
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4172..4228
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4244..4300
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4316..4372
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4388..4444
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4460..4516
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4532..4588
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4604..4660
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     primer_bind     complement(4884..4904)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5069..5204
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5228..5602
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     misc_feature    5688..6276
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5741..5761)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6159..6170)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6417..6597
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    6669..6790
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      6809..6944
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6964..6983)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6965..6983)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6993..7010)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6993..7009)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7002..7024)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7151..7606
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7238..7257)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7448..7469
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7632..7736
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7737..7805,7806..8597)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7955..7974)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8768..9356
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9257..9276
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9510..9527
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9644..9665
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9680..9685,9686..9703,9704..9710)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9718..9734
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9723..9745
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9742..9758
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9742..9758
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9777..9797
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9779..9797
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        9825..10051
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             10052..10232
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    10279..10404
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10897..11130
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11315..11359
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    11626..11743
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 tcacgagact agcctcgata gcccgggctc gaccatatga taagctttgc aaagatggat
       61 aaagttttaa acagagagga atctttgcag ctaatggacc ttctaggtct tgaaaggagt
      121 gggaattggc tccggtgccc gtcagtgggc agagcgcaca tcgcccacag tccccgagaa
      181 gttgggggga ggggtcggca attgaaccgg tgcctagaga aggtggcgcg gggtaaactg
      241 ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag ggtgggggag aaccgtatat
      301 aagtgcagta gtcgccgtga acgttctttt tcgcaacggg tttgccgcca gaacacaggt
      361 aagtgccgtg tgtggttccc gcgggcctgg cctctttacg ggttatggcc cttgcgtgcc
      421 ttgaattact tccacctggc tgcagtacgt gattcttgat cccgagcttc gggttggaag
      481 tgggtgggag agttcgaggc cttgcgctta aggagcccct tcgcctcgtg cttgagtyga
      541 ggcctggcct gggcgctggg gccgccgcgt gcgaatctgg tggcaccttc gcgcctgtct
      601 cgctgctttc gataagtctc tagccattta aaatttttga tgacctgctg cgacgctttt
      661 tttctggcaa gatagtcttg taaatgcggg ccaagatctg cacactggta tttcggtttt
      721 tggggccgcg ggcggcgacg gggcccgtgc gtcccagcgc acatgttcgg cgaggcgggg
      781 cctgcgagcg cggccaccga gaatcggacg gggggagtct caagctggcc ggcctgccct
      841 ggtgccyggc ctcgcgccgc cgtgtatcgc cccgccctgg gcggcaaggc tggcccggtc
      901 ggcaccagtt gcgtgagcgg aaagatggcc gcttcccggc cctgctgcag ggagctcaaa
      961 atggaggacg cggcgctcgg gagagcgggc gggtgagtca cccacacaaa ggaaaagggc
     1021 ctttccgtcc tcagccgtcg cttcatgtga ctccacggag taccgggcgc cgtccaggca
     1081 cctcgattag ttctcgagct tttggagtac gtcgtcttta ggttgggggg aggggtttta
     1141 tgcgatggag tttccccaca ctgagtgggt ggagactgaa gttaggccag cttggcactt
     1201 gatgtaattc tcctttctag agccaccatg ggtggcgcgc ctccaaaaaa gaagagaaag
     1261 gtagatccaa aaaagaagag aaaggtagat ccaaaaaaga agagaaaggt agrwyyaaaa
     1321 aagaagagaa aggtaggatt aattaatgga agtggaagtg gaagtggagg taccatgtcg
     1381 cggacccggc tcccttcccc acccgcaccc agcccagcgt tttcggccga ctcgttctca
     1441 gacctgctta ggcagttcga cccctcactg tttaacacat cgttgttcga ctcccttcct
     1501 ccgtttgggg cgcaccatac ggaggcggcc accggggagt gggatgaggt gcagtcggga
     1561 ttgagagctg cggatgcacc acccccaacc atgcgggtgg ccgtcaccgc tgcccgaccg
     1621 ccgagggcga agcccgcacc aaggcggagg gcagcgcaac cgtccgacgc aagccccgca
     1681 gcgcaagtag atttgagaac tttgggatat tcacagcagc agcaggaaaa gatcaagccc
     1741 aaagtgaggt cgacagtcgc gcagcatcac gaagcgctgg tgggtcatgg gtttacacat
     1801 gcccacatcg tagccttgtc gcagcaccct gcagcccttg gcacggtcgc cgtcaagtac
     1861 caggacatga ttgcggcgtt gccggaagcc acacatgagg cgatcgtcgg tgtggggaaa
     1921 cagtggagcg gagcccgagc gcttgaggcc ctgttgacgg tcgcgggaga gctgagaggg
     1981 cctccccttc agckggacmc gggccagttg ctgaagmtcg cgaagcgggg wgkrktcams
     2041 gcnggtcgag ggggggcacg cgcggsgsmr wgsscysacg ggggcacccc tcaacchgvc
     2101 cccagagcag gtcgtggcca ttgcctcgaa tggagggggc aaacaggcgt tggaaaccgt
     2161 acaacgattg ctgccggtgc tgtgccaagc gcacggactc accccagagc aggtcgtggc
     2221 cattgcctcg aatggagggg gcaaacaggc gttggaaacc gtacaacgat tgctgccggt
     2281 gctgtgccaa gcgcacggcc tcaccccaga gcaggtcgtg gcaatcgcct ccaacattgg
     2341 cgggaaacag gcactcgaga ctgtccagcg cctgcttccc gtgctgtgcc aagcgcacgg
     2401 attaacccca gagcaggtcg tggccattgc ctcgaatgga gggggcaaac aggcgttgga
     2461 aaccgtacaa cgattgctgc cggtgctgtg ccaagcgcac ggcttaaccc cagagcaggt
     2521 cgtggccatt gcctcgaatg gagggggcaa acaggcgttg gaaaccgtac aacgattgct
     2581 gccggtgctg tgccaagcgc acggacttac cccagagcag gtcgtggcga tcgcaagcca
     2641 cgacggagga aagcaagcct tggaaacagt acagaggctg ttgcctgtgc tgtgccaagc
     2701 gcacggcctt accccagagc aggtcgtggc gatcgcaagc cacgacggag gaaagcaagc
     2761 cttggaaaca gtacagaggc tgttgcctgt gctgtgccaa gcgcacggac taaccccaga
     2821 gcaggtcgtg gcgatcgcaa gccacgacgg aggaaagcaa gccttggaaa cagtacagag
     2881 gctgttgcct gtgctgtgcc aagcgcacgg gctcacccca gagcaggtcg tggccattgc
     2941 ctcgaatgga gggggcaaac aggcgttgga aaccgtacaa cgattgctgc cggtgctgtg
     3001 ccaagcgcac gggctaaccc cagagcaggt cgtggcaatc gcgagcaata acggcggaaa
     3061 acaggctttg gaaacggtgc agaggctcct tccagtgctg tgccaagcgc acggcctaac
     3121 cccagagcag gtcgtggcaa tcgcctccaa cattggcggg aaacaggcac tcgagactgt
     3181 ccagcgcctg cttcccgtgc tgtgccaagc gcacgggtta accccagagc aggtcgtggc
     3241 gatcgcaagc cacgacggag gaaagcaagc cttggaaaca gtacagaggc tgttgcctgt
     3301 gctgtgccaa gcgcacggac tcacgcctga gcaggtagtg gctattgcat ccaatatcgg
     3361 gggcagaccc gcactggagt caatcgtggc ccagctttcg aggccggacc ccgcgctggc
     3421 cgcactcact aatgatcatc ttgtagcgct ggcctgcctc ggcggacgac ccgccttgga
     3481 tgcggtgaag aaggggctcc cgcacgcgcc tgcattgatt aagcggacca acagaaggat
     3541 tcccgagagg acatcacatc gagtggcaga tcacgcgcaa gtggtccgcg tgctcggatt
     3601 cttccagtgt cactcccacc ccgcacaagc gttcgatgac gccatgactc aatttggtat
     3661 gtcgagacac ggactgctgc agctctttcg tagagtcggt gtcacagaac tcgaggcccg
     3721 ctcgggcaca ctgcctcccg cctcccagcg gtgggacagg attctccaag cgagcggtat
     3781 gaaacgcgcg aagccttcac ctacgtcaac tcagacacct gaccaggcga gccttcatgc
     3841 gttcgcagac tcgctggaga gggatttgga cgcgccctcg cccatgcatg aaggggacca
     3901 aactcgcgcg tcagctggat ccgccatcat acacgcggcc gcaacaagcg gtccggaaga
     3961 acttttgagc aagaattatc atcttgagaa cgaagtggct cgtcttaaga aaggttctgg
     4021 cagtggagaa gaactgcttt caaagaatta ccacctggaa aatgaggtag ctagactgaa
     4081 aaaggggagc ggaagtgggg aggagttgct tagcaaaaat tatcatttgg agaacgaagt
     4141 agcacgacta aagaaagggt ccggatcggg tgaggagtta ctctcgaaaa attatcatct
     4201 cgaaaacgaa gtggctcggc taaaaaaggg cagtggttct ggagaagagc tattatctaa
     4261 aaactaccac ctcgaaaatg aggtggcacg cttaaaaaag ggaagtggca gtggtgaaga
     4321 gctactatcc aagaattatc atcttgagaa cgaggtagcg cgtttgaaga agggttccgg
     4381 ctcaggagag gaactgctct cgaagaacta tcatcttgaa aatgaggtcg ctcgattaaa
     4441 aaagggatcg ggcagtggtg aggaactact ttcaaagaat taccacctcg aaaacgaagt
     4501 agctcgatta aagaaaggtt cagggtcggg tgaagaatta ctgagtaaaa attatcatct
     4561 ggaaaatgag gtagcgagac taaaaaaggg gagtggttct ggcgaggaat tgctatcgaa
     4621 aaattatcat cttgagaacg aagttgctag gctcaaaaag ggctcaggct caggctgagt
     4681 ttaaacatcg cctgcagggg aagtggaagt gatatcggaa gtggaagcac tagtggaagt
     4741 ttcgaagtcg acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     4801 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     4861 aatgtatctt atgtcgaccc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg
     4921 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg
     4981 aaagtcccca ggctccccag caggcagamc wawctcatca atgtatctta tcatgtctgg
     5041 ctctagcttc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     5101 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
     5161 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggga cktgcaaaaa
     5221 gcttaccatg gccaagttga ccagtgccgt tccggtgctc accgcgcgcg acgtcgccgg
     5281 agcggtcgag ttctggaccg accggctcgg gttctcccgg gacttcgtgg aggacgactt
     5341 cgccggtgtg gtccgggacg acgtgaccct gttcatcagc gcggtccagg accaggtggt
     5401 gccggacaac accctggcct gggtgtgggt gcgcggcctg gacgagctgt acgccgagtg
     5461 gtcggaggtc gtgtccacga acttccggga cgcctccggg ccggccatga ccgagatcgg
     5521 cgagcagccg tgggggcggg agttcgccct gcgcgacccg gccggcaact gcgtgcactt
     5581 cgtggccgag gagcaggact gaccgatcga taaaataaaa gattttattt agtctccaga
     5641 aaaagggggg aatgaaagac cccacctgta ggtttggcaa gctcgacaat caacctctgg
     5701 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
     5761 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
     5821 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca
     5881 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg
     5941 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg
     6001 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
     6061 attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca
     6121 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc
     6181 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc
     6241 agacgagtcg gatctccctt tgggccgcct ccccgcctgg aattcgagct cagtaccttt
     6301 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg
     6361 actggaaggg gcattgcaga gatattgtat ttaagtgcct agctcgatac ataaacgggt
     6421 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     6481 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     6541 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta
     6601 gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga
     6661 gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     6721 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     6781 atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca gttccgccca
     6841 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
     6901 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggga cgtacccaat
     6961 tcgccctata gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac
     7021 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc
     7081 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat
     7141 ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     7201 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     7261 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     7321 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     7381 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     7441 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     7501 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     7561 caaaaattta acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc
     7621 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     7681 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     7741 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     7801 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     7861 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     7921 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     7981 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     8041 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     8101 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     8161 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     8221 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     8281 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     8341 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     8401 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     8461 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     8521 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     8581 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     8641 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     8701 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     8761 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     8821 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     8881 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     8941 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     9001 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     9061 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     9121 gaacgaccta caccaaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     9181 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     9241 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     9301 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     9361 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     9421 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     9481 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     9541 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     9601 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
     9661 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
     9721 tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc aagcgcgcaa
     9781 ttaaccctca ctaaagggaa caaaagctgg agctgcaagc ttaatgtagt cttatgcaat
     9841 actcttgtag tcttgcaaca tggtaacgat gagttagcaa catgccttac aaggagagaa
     9901 aaagcaccgt gcatgccgat tggtggaagt aaggtggtac gatcgtgcct tattaggaag
     9961 gcaacagacg ggtctgacat ggattggacg aaccactgaa ttgccgcatt gcagagatat
    10021 tgtatttaag tgcctagctc gatacataaa cgggtctctc tggttagacc agatctgagc
    10081 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
    10141 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
    10201 acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca
    10261 gtatttataa cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca
    10321 agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag
    10381 gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa
    10441 aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca
    10501 agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt
    10561 agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca
    10621 ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc
    10681 aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac cgcacagcaa
    10741 gcggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt
    10801 atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag
    10861 aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt
    10921 gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca
    10981 attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca
    11041 gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt
    11101 ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat
    11161 ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg
    11221 gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca
    11281 ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt
    11341 agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa
    11401 attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc
    11461 tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc acctcccaac
    11521 cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga gagacagaga
    11581 cagatccatt cgattagtga acggatctcg acggtatcgg ttaactttta aaagaaaagg
    11641 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
    11701 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttatcgat
//
