LOCUS       sequence-24108-e        1113 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1113)
  TITLE     T4 Lysozyme Plasmids - Tray 2
REFERENCE   2  (bases 1 to 1113)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: T4 Lysozyme S44-[AAAA] WT*
FEATURES             Location/Qualifiers
     source          1..1113
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    734..750
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(758..764,765..780,781..786))
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    829..845
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(853..859,860..875,876..881))
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     gap             1016..1030
                     /estimated_length=15
ORIGIN
        1 ctangaaaag cccgctcatt aggcgggctc agatctgctc atgtttgaca gcttatcatc
       61 gataagcttc ggcaataaat tcttgatatg ttttcatatg tttcctttaa atattttaat
      121 tagtaattgt ctattcaagt aattgtgaat atactatcac aattccaaga gaaagtaaac
      181 agctttatag atttttatac gcgtcccaag tgccagttct aaacgttgta atgactcgtt
      241 ttgcgcgatt aggtgtttga ttataccatc tacttttagc taagttaact gctgcttcat
      301 cccagcgttt ttgttgaagc atacgtaaag agttagtaaa tcctgccaca ccggtttctc
      361 ccatttggaa aaccatatta atcaatgcag cgcgacgaac cgcatcaaga gaatcataaa
      421 ccggttttaa tttagcattt ctcagaattc cgcgaacagc agcatcaaca tcctgattaa
      481 agagtttttc agcctcatct tttgtaatta caccattagt attacgccca atagctttat
      541 ctaattctgc agctgcagca gatttagcag cattaagtga tggacttttt gtaagcaaat
      601 gaccgatgcc aatagtgtaa tagccttctg tgtctttata gattttaagt ctaagacctt
      661 catctatacg taacatttca aatatattca taatacctcc taagcatcga tggatcctgt
      721 ttcctgtgtg aaattgttat ccgctcacaa ttccacacat tatacgagcc gatgattaat
      781 tgtcaacagg gggatgggga gtaagctgat cctgtttcct gtgtgaaatt gttatccgct
      841 cacaattcca cacattatac gagccgatga ttaattgtca acagggggat ggggagtaag
      901 ctcatcgatg gatcgatccg gtttcccggn gngaaanngn nanccgcncn canntccccn
      961 cnttanncna nccggaancn nnannngnna anccgggggg gncnnangnn ngnncnnnnn
     1021 nnnnnnnnnn gnnnngnnnn nnnggcccnn ntnccnnnnn ggnaacnngn nnggcnngnn
     1081 nccnnnnnnn gggggnnnan cnntnnnggn nng
//
