LOCUS       sequence-241086-        6461 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Targeting expression to neuronal and glial cell types in mice, 
            non-human primates, and human retina.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6461)
  AUTHORS   Juttner J, Szabo A, Gross-Scherf B, Morikawa RK, Rompani SB, Hantz 
            P, Szikra T, Esposti F, Cowan CS, Bharioke A, Patino-Alvarez CP, 
            Keles O, Kusnyerik A, Azoulay T, Hartl D, Krebs AR, Schubeler D, 
            Hajdu RI, Lukats A, Nemeth J, Nagy ZZ, Wu KC, Wu RH, Xiang L, Fang 
            XL, Jin ZB, Goldblum D, Hasler PW, Scholl HPN, Krol J, Roska B
  TITLE     Targeting neuronal and glial cell types with synthetic promoter AAVs
            in mice, non-human primates and humans.
  JOURNAL   Nat Neurosci. 2019 Jul 8. pii: 10.1038/s41593-019-0431-2. doi: 
            10.1038/s41593-019-0431-2.
  PUBMED    31285614
REFERENCE   2  (bases 1 to 6461)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2019 Jul 8. pii: 10.1038/s41593-019-0431-2. doi: 
            10.1038/s41593-019-0431-2."
COMMENT     Sequence Label: 119_pAAV-ProC25-CatCh-GFP-WPRE
FEATURES             Location/Qualifiers
     source          1..6461
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     promoter        702..898
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      749..884
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     regulatory      905..914
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             911..1837
                     /codon_start=1
                     /product="N-terminal fragment of channelrhodopsin-2 from 
                     the alga Chlamydomonas reinhardtii (Nagel et al., 2003)"
                     /label=ChR2
                     /note="blue light-activated cation channel"
                     /translation="MDYGGALSAVGRELLFVTNPVVVNGSVLVPEDQCYCAGWIESRGT
                     NGAQTASNVLQWLAAGFSILLLMFYAYQTWKSTCGWEEIYVCAIEMVKVILEFFFEFKN
                     PSMLYLATGHRVQWLRYAEWLLTCPVICIHLSNLTGLSNDYSRRTMGLLVSDIGTIVWG
                     ATSAMATGYVKVIFFCLGLCYGANTFFHAAKAYIEGYHTVPKGRCRQVVTGMAWLFFVS
                     WGMFPILFILGPEGFGVLSVYGSTVGHTIIDLMSKNCWGLLGHYLRVLIHEHILIHGDI
                     RKTTKLNIGGTEIEVETLVEDEAEAGAV"
     CDS             join(1841..1843,1844..1846,1847..2560)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1841..1843,1844..1846,1847..2557)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1886..1907)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2147..2166)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2494..2515
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    2588..3176
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2641..2661)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3059..3070)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3208..3684
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(3407..3426)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   3724..3864
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   3724..3853
                     /label=AAV2 ITR
     rep_origin      3939..4394
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4026..4045)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4236..4257
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4411..4430)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4530..4552
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4590..4608)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4676..4780
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4781..4849,4850..5641)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4999..5018)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5812..6400
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6301..6320
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtcagtta accttagtag gctatattta aatgttcaat
      181 aatatgggct atatttaagc taggagtcca cctggctata tttaaagcgt ggtattatct
      241 ggctatattt aagcggggtg cactgtgggc tatatttaat aggcggtcga cgatggctat
      301 atttaacgca cgctgggtac cggctatatt taagctcaag tgttgagggg ctatatttaa
      361 agtcgaaagt tcagtggcta tatttaagtg cctgtccctt ctggctatat ttaagctcgt
      421 tacttaagag gctatattta atacctaatt catagaggct atatttaagc gaaagtaaaa
      481 gatggctata tttaaatttc gattctgatg ggctatattt aataaaggaa acagtcgggc
      541 tatatttaat cgccacggat ttccggctat atttaatgac atccagcatt tggctatatt
      601 taagggaggt gtacttccgg ctatatttaa gaaataatat aacgtggcta tatttaattc
      661 caacataccc taggctatat ttaagctcga gatctgcgat ctgcatctca attagtcagc
      721 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
      781 ttctccgccc catcgctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
      841 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaagg
      901 atccgccacc atggattatg gaggcgccct gagtgccgtt gggcgcgagc tgctatttgt
      961 aacgaaccca gtagtcgtca atggctctgt acttgtgcct gaggaccagt gttactgcgc
     1021 gggctggatt gagtcgcgtg gcacaaacgg tgcccaaacg gcgtcgaacg tgctgcaatg
     1081 gcttgctgct ggcttctcca tcctactgct tatgttttac gcctaccaaa catggaagtc
     1141 aacctgcggc tgggaggaga tctatgtgtg cgctatcgag atggtcaagg tgattctcga
     1201 gttcttcttc gagtttaaga acccgtccat gctgtatcta gccacaggcc accgcgtcca
     1261 gtggttgcgt tacgccgagt ggcttctcac ctgcccggtc atttgcattc acctgtcaaa
     1321 cctgacgggc ttgtccaacg actacagcag gcgcaccatg ggtctgcttg tgtctgatat
     1381 tggcacaatt gtgtggggcg ccacttccgc catggccacc ggatacgtca aggtcatctt
     1441 cttctgcctg ggtctgtgtt atggtgctaa cacgttcttt cacgctgcca aggcctacat
     1501 cgagggttac cacaccgtgc cgaagggccg gtgtcgccag gtggtgactg gcatggcttg
     1561 gctcttcttc gtatcatggg gtatgttccc catcctgttc atcctcggcc ccgagggctt
     1621 cggcgtcctg agcgtgtacg gctccaccgt cggccacacc atcattgacc tgatgtcgaa
     1681 gaactgctgg ggtctgctcg gccactacct gcgcgtgctg atccacgagc atatcctcat
     1741 ccacggcgac attcgcaaga ccaccaaatt gaacattggt ggcactgaga ttgaggtcga
     1801 gacgctggtg gaggacgagg ccgaggctgg cgcggtaccc atggtgagca agggcgagga
     1861 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa
     1921 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt
     1981 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta
     2041 cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc
     2101 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta
     2161 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa
     2221 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa
     2281 cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa
     2341 gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac
     2401 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc
     2461 cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
     2521 cgccgggatc actctcggca tggacgagct gtacaagtga taagaattcg atatcaagct
     2581 tatcgataat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     2641 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
     2701 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
     2761 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
     2821 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
     2881 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
     2941 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg
     3001 gctgctcgcc tatgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
     3061 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
     3121 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcatcg
     3181 ataccgagcg ctgctcgaga gatctacggg tggcatccct gtgacccctc cccagtgcct
     3241 ctcctggccc tggaagttgc cactccagtg cccaccagcc ttgtcctaat aaaattaagt
     3301 tgcatcattt tgtctgacta ggtgtccttc tataatatta tggggtggag gggggtggta
     3361 tggagcaagg ggcaagttgg gaagacaacc tgtagggcct gcggggtcta ttgggaacca
     3421 agctggagtg cagtggcaca atcttggctc actgcaatct ccgcctcctg ggttcaagcg
     3481 attctcctgc ctcagcctcc cgagttgttg ggattccagg catgcatgac caggctcagc
     3541 taatttttgt ttttttggta gagacggggt ttcaccatat tggccaggct ggtctccaac
     3601 tcctaatctc aggtgatcta cccaccttgg cctcccaaat tgctgggatt acaggcgtga
     3661 accactgctc ccttccctgt ccttctgatt ttgtaggtaa ccacgtgcgg accgagcggc
     3721 cgcaggaacc cctagtgatg gagttggcca ctccctctct gcgcgctcgc tcgctcactg
     3781 aggccgggcg accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg
     3841 agcgagcgcg cagctgcctg caggggcgcc tgatgcggta ttttctcctt acgcatctgt
     3901 gcggtatttc acaccgcata cgtcaaagca accatagtac gcgccctgta gcggcgcatt
     3961 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     4021 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     4081 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     4141 caaaaaactt gatttgggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     4201 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     4261 aacactcaac cctatctcgg gctattcttt tgatttataa gggattttgc cgatttcggc
     4321 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt
     4381 aacgtttaca attttatggt gcactctcag tacaatctgc tctgatgccg catagttaag
     4441 ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc
     4501 atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc
     4561 gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa
     4621 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
     4681 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     4741 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     4801 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     4861 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     4921 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     4981 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     5041 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     5101 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     5161 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     5221 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     5281 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     5341 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     5401 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     5461 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     5521 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     5581 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     5641 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     5701 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     5761 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     5821 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     5881 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     5941 gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     6001 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     6061 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     6121 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     6181 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     6241 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     6301 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     6361 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     6421 tttacggttc ctggcctttt gctggccttt tgctcacatg t
//
