LOCUS       sequence-241097-        6401 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Targeting expression to neuronal and glial cell types in mice, 
            non-human primates, and human retina.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6401)
  AUTHORS   Juttner J, Szabo A, Gross-Scherf B, Morikawa RK, Rompani SB, Hantz 
            P, Szikra T, Esposti F, Cowan CS, Bharioke A, Patino-Alvarez CP, 
            Keles O, Kusnyerik A, Azoulay T, Hartl D, Krebs AR, Schubeler D, 
            Hajdu RI, Lukats A, Nemeth J, Nagy ZZ, Wu KC, Wu RH, Xiang L, Fang 
            XL, Jin ZB, Goldblum D, Hasler PW, Scholl HPN, Krol J, Roska B
  TITLE     Targeting neuronal and glial cell types with synthetic promoter AAVs
            in mice, non-human primates and humans.
  JOURNAL   Nat Neurosci. 2019 Jul 8. pii: 10.1038/s41593-019-0431-2. doi: 
            10.1038/s41593-019-0431-2.
  PUBMED    31285614
REFERENCE   2  (bases 1 to 6401)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2019 Jul 8. pii: 10.1038/s41593-019-0431-2. doi: 
            10.1038/s41593-019-0431-2."
COMMENT     Sequence Label: 124_pAAV-ProC3-CatCh-GFP-WPRE
FEATURES             Location/Qualifiers
     source          1..6401
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     promoter        642..838
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      689..824
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     regulatory      845..854
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             851..1777
                     /codon_start=1
                     /product="N-terminal fragment of channelrhodopsin-2 from 
                     the alga Chlamydomonas reinhardtii (Nagel et al., 2003)"
                     /label=ChR2
                     /note="blue light-activated cation channel"
                     /translation="MDYGGALSAVGRELLFVTNPVVVNGSVLVPEDQCYCAGWIESRGT
                     NGAQTASNVLQWLAAGFSILLLMFYAYQTWKSTCGWEEIYVCAIEMVKVILEFFFEFKN
                     PSMLYLATGHRVQWLRYAEWLLTCPVICIHLSNLTGLSNDYSRRTMGLLVSDIGTIVWG
                     ATSAMATGYVKVIFFCLGLCYGANTFFHAAKAYIEGYHTVPKGRCRQVVTGMAWLFFVS
                     WGMFPILFILGPEGFGVLSVYGSTVGHTIIDLMSKNCWGLLGHYLRVLIHEHILIHGDI
                     RKTTKLNIGGTEIEVETLVEDEAEAGAV"
     CDS             join(1781..1783,1784..1786,1787..2500)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1781..1783,1784..1786,1787..2497)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1826..1847)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2087..2106)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2434..2455
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    2528..3116
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2581..2601)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2999..3010)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3148..3624
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(3347..3366)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   3664..3804
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   3664..3793
                     /label=AAV2 ITR
     rep_origin      3879..4334
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3966..3985)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4176..4197
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4351..4370)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4470..4492
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4530..4548)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4616..4720
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4721..4789,4790..5581)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4939..4958)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5752..6340
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6241..6260
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtaaagct ggaactgggg ccggaaacag ctgagggtgc
      181 ccagccggaa actcgaaatc aacgtaggcc ggaaactatt cgatgaattc cgccggaaac
      241 taggtccgga ggactgccgg aaacacctgt aatcaagccg ccggaaacct gttgtggccg
      301 tatgccggaa acgtcttaat tggacgtgcc ggaaactctt ttaatgagtt cgccggaaac
      361 cagaccagcc gagctgccgg aaaccggtta tatagaacgg ccggaaacgg tccacaggaa
      421 aaagccggaa acacccaaac ggttagcgcc ggaaacgact ggggaggacg tgccggaaac
      481 gtactatctg aagatgccgg aaacacttga aagctccaag ccggaaacgg gcccgtgcgg
      541 atagccggaa actgacggta cacggccgcc ggaaacacta cttgtatggt agccggaaac
      601 ttgggcgtgg ctggggccgg aaacgctcga gatctgcgat ctgcatctca attagtcagc
      661 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
      721 ttctccgccc catcgctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
      781 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaagg
      841 atccgccacc atggattatg gaggcgccct gagtgccgtt gggcgcgagc tgctatttgt
      901 aacgaaccca gtagtcgtca atggctctgt acttgtgcct gaggaccagt gttactgcgc
      961 gggctggatt gagtcgcgtg gcacaaacgg tgcccaaacg gcgtcgaacg tgctgcaatg
     1021 gcttgctgct ggcttctcca tcctactgct tatgttttac gcctaccaaa catggaagtc
     1081 aacctgcggc tgggaggaga tctatgtgtg cgctatcgag atggtcaagg tgattctcga
     1141 gttcttcttc gagtttaaga acccgtccat gctgtatcta gccacaggcc accgcgtcca
     1201 gtggttgcgt tacgccgagt ggcttctcac ctgcccggtc atttgcattc acctgtcaaa
     1261 cctgacgggc ttgtccaacg actacagcag gcgcaccatg ggtctgcttg tgtctgatat
     1321 tggcacaatt gtgtggggcg ccacttccgc catggccacc ggatacgtca aggtcatctt
     1381 cttctgcctg ggtctgtgtt atggtgctaa cacgttcttt cacgctgcca aggcctacat
     1441 cgagggttac cacaccgtgc cgaagggccg gtgtcgccag gtggtgactg gcatggcttg
     1501 gctcttcttc gtatcatggg gtatgttccc catcctgttc atcctcggcc ccgagggctt
     1561 cggcgtcctg agcgtgtacg gctccaccgt cggccacacc atcattgacc tgatgtcgaa
     1621 gaactgctgg ggtctgctcg gccactacct gcgcgtgctg atccacgagc atatcctcat
     1681 ccacggcgac attcgcaaga ccaccaaatt gaacattggt ggcactgaga ttgaggtcga
     1741 gacgctggtg gaggacgagg ccgaggctgg cgcggtaccc atggtgagca agggcgagga
     1801 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa
     1861 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt
     1921 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta
     1981 cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc
     2041 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta
     2101 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa
     2161 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa
     2221 cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa
     2281 gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac
     2341 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc
     2401 cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
     2461 cgccgggatc actctcggca tggacgagct gtacaagtga taagaattcg atatcaagct
     2521 tatcgataat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     2581 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
     2641 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
     2701 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
     2761 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
     2821 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
     2881 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg
     2941 gctgctcgcc tatgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
     3001 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
     3061 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcatcg
     3121 ataccgagcg ctgctcgaga gatctacggg tggcatccct gtgacccctc cccagtgcct
     3181 ctcctggccc tggaagttgc cactccagtg cccaccagcc ttgtcctaat aaaattaagt
     3241 tgcatcattt tgtctgacta ggtgtccttc tataatatta tggggtggag gggggtggta
     3301 tggagcaagg ggcaagttgg gaagacaacc tgtagggcct gcggggtcta ttgggaacca
     3361 agctggagtg cagtggcaca atcttggctc actgcaatct ccgcctcctg ggttcaagcg
     3421 attctcctgc ctcagcctcc cgagttgttg ggattccagg catgcatgac caggctcagc
     3481 taatttttgt ttttttggta gagacggggt ttcaccatat tggccaggct ggtctccaac
     3541 tcctaatctc aggtgatcta cccaccttgg cctcccaaat tgctgggatt acaggcgtga
     3601 accactgctc ccttccctgt ccttctgatt ttgtaggtaa ccacgtgcgg accgagcggc
     3661 cgcaggaacc cctagtgatg gagttggcca ctccctctct gcgcgctcgc tcgctcactg
     3721 aggccgggcg accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg
     3781 agcgagcgcg cagctgcctg caggggcgcc tgatgcggta ttttctcctt acgcatctgt
     3841 gcggtatttc acaccgcata cgtcaaagca accatagtac gcgccctgta gcggcgcatt
     3901 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     3961 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     4021 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     4081 caaaaaactt gatttgggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     4141 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     4201 aacactcaac cctatctcgg gctattcttt tgatttataa gggattttgc cgatttcggc
     4261 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt
     4321 aacgtttaca attttatggt gcactctcag tacaatctgc tctgatgccg catagttaag
     4381 ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc
     4441 atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc
     4501 gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa
     4561 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
     4621 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     4681 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     4741 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     4801 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     4861 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     4921 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     4981 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     5041 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     5101 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     5161 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     5221 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     5281 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     5341 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     5401 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     5461 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     5521 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     5581 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     5641 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     5701 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     5761 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     5821 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     5881 gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     5941 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     6001 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     6061 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     6121 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     6181 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     6241 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     6301 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     6361 tttacggttc ctggcctttt gctggccttt tgctcacatg t
//
