LOCUS       sequence-241098-       11767 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of XRN1__PRKCE fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11767)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 11767)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-XRN1__PRKCE
FEATURES             Location/Qualifiers
     source          1..11767
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2223..3401
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2454..3392
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3349..3369
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    3421..3445
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6649..6673)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6827..6847)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             6901..6942
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    6963..7530
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    6980..7532
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7124..7141)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7351..7370
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7544..8143
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7544..7563)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8040..8060
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    8157..8745
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8210..8230)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8628..8639)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8820..9053
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(9172..9190)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9258..9362
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9363..9431,9432..10223)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9581..9600)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10394..10982
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10883..10902
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11136..11153
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11393..11414
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11429..11434,11435..11452,11453..11459)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11467..11483
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11472..11494
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11491..11507
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11491..11507
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11557..11576
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccct gcagatatca
     3421 acaagtttgt acaaaaaagt tggcatggga gtccccaagt tttacagatg gatctcagag
     3481 cggtatccct gtctcagcga agtggtgaaa gagcatcaga ttcctgaatt tgacaacttg
     3541 tacctggata tgaatggaat tatacatcag tgctcccatc ctaatgatga tgatgttcac
     3601 tttagaattt cagatgataa aatctttact gatatttttc actacctgga ggtgttgttt
     3661 cgcattatta aacccaggaa agtgttcttt atggctgtag atggtgtggc tcctcgagca
     3721 aaaatgaacc agcagcgtgg gaggcgtttt aggtcagcaa aggaggcaga agacaaaatt
     3781 aaaaaggcaa tagagaaggg agaaactctt cctacagagg ccagatttga ttccaactgt
     3841 atcacaccag gaactgaatt tatggccagg ttacatgaac atctgaagta ttttgtaaat
     3901 atgaaaattt ccacagacaa gtcatggcaa ggagttacca tctacttctc aggccatgag
     3961 actcctggag aaggagagca taaaatcatg gaatttatca gatccgagaa agcaaagcca
     4021 gatcatgatc caaacaccag acactgtctt tatggtttag atgctgactt gattatgctt
     4081 ggattaacaa gtcatgaggc acatttttct ctcttaagag aagaagttcg atttggtggc
     4141 aaaaaaacac aacgggtatg tgctccagaa gaaactacat ttcaccttct acacttgtct
     4201 ttaatgagag agtatattga ctatgagttt tcagtattaa aagaaaagat cacatttaaa
     4261 tatgatattg aaaggataat agatgattgg attttgatgg ggtttcttgt tggtaatgat
     4321 tttatccctc atctacctca tttacatatt aatcatgatg cactgcctct tctttatgga
     4381 acatatgtta ccatcctgcc agaacttggg ggttatatta atgaaagtgg gcacctcaac
     4441 ttacctcgat ttgagaaata ccttgtgaaa ctatcagatt ttgatcggga gcacttcagt
     4501 gaagtttttg tggacctaaa atggtttgaa agcaaagttg gtaacaagta cctcaatgaa
     4561 gcagcaggtg tcgcagcaga agaagccagg aactacaagg aaaagaaaaa gttaaagggc
     4621 caggaaaatt ctctgtgttg gactgcttta gacaaaaatg aaggcgaaat gataacttct
     4681 aaggataatt tagaagatga gactgaagat gatgacctat ttgaaactga gtttagacaa
     4741 tataaaagaa catattacat gacgaagatg ggggttgacg tagtatctga tgactttctg
     4801 gctgatcaag ctgcatgtta tgttcaggca atacagtgga ttttgcacta ttactatcat
     4861 ggagttcagt cctggagctg gtattatcct tatcattatg cacctttcct gtctgatata
     4921 cacaacatca gtacactcaa aatccatttt gaactaggaa aaccttttaa gccatttgaa
     4981 cagcttcttg ctgtacttcc agcagccagc aaaaatttac ttcctgcatg ctaccagcat
     5041 ttgatgacca atgaagactc accaattata gaatattacc cacctgattt taaaactgac
     5101 ctaaatggga aacaacagga atgggaagct gtggtgttaa tcccttttat tgatgagaag
     5161 cgattattgg aagccatgga gacatgtaac cactccctca aaaaggaaga gaggaaaaga
     5221 aaccaacata gtgagtgcct aatgtgctgg tatgatagag acacagagtt tatctatcct
     5281 tctccatggc cagaaaagtt ccctgccata gaacgatgtt gtacaaggta taaaataata
     5341 tccttagatg cttggcgtgt agacataaac aaaaacaaaa taaccagaat tgaccagaaa
     5401 gcattatatt tctgtggatt tcctactctg aaacacatca gacacaaatt ttttttgaag
     5461 aaaagtggtg ttcaagtatt ccagcaaagc agtcgtggag aaaacatgat gttggaaatc
     5521 ttagtggatg cagaatcaga tgaacttacc gtagaaaatg tagcttcatc agtgcttgga
     5581 aaatctgtct ttgttaattg gcctcacctt gaggaagcta gagtcgtggc tgtatcagat
     5641 ggagaaacta agttttactt ggaagaacct ccaggaacac agaagcttta ttcaggaaga
     5701 actgccccac catctaaagt ggttcatctt ggagataaag aacaatctaa ctgggcaaaa
     5761 gaagtacaag gaatttcaga acactacctg agaagaaaag gaataataat aaatgaaaca
     5821 tctgcagttg tgtatgctca gttactcaca ggtcgtaaat atcaaataaa tcaaaatggt
     5881 gaagttcgtc tagagaaaca gtggtcaaaa caagttgttc cttttgttta tcaaactatt
     5941 gtcaaggaca tccgagcttt cgactcccgt ttctccaata tcaaaacatt ggatgatttg
     6001 tttcctctga gaagtatggt ctttatgctg ggaactccct attatggctg cactggagaa
     6061 gttcaggatt caggtgatgt gattacagaa ggtaggattc gtgtgatttt cagcattcca
     6121 tgtgaaccca atcttgatgc tttaatacag aaccagcata aatattctat aaagtacaac
     6181 ccaggatatg tgttggccag tcgccttgga gtgagtggat accttgtttc aaggtttaca
     6241 ggaagtattt ttattggaag aggatctagg agaaaccctc atggagacca taaagcaaat
     6301 gtgggtttaa atctcaaatt caacaagaaa aatgaggagg tacctggata tactaagaaa
     6361 gttggaagtg aatggatgta ttcatctgca gcagaacaac ttctggcaga gtacttagag
     6421 agagctccag aactatttag ttatatagcc aaaaatagcc aagaggatgt gttctatgaa
     6481 gatgacattt ggcctggaga aaatgagaat ggtgctgaaa aagttcaaga aattattact
     6541 tggctaaaag gacatcctgt cagtacttta tctcgttctt cttgtgattt acaaattctg
     6601 gatgcagcta ttgttgagaa aattgaggaa gaagtcgaaa agtgcaagtc ccagctttct
     6661 tgtacaaagt ggttcaaaac caaaagagac gtcaataatt ttgaccaaga ctttacccgg
     6721 gaagagccgg tactcaccct tgtggacgaa gcaattgtaa agcagatcaa ccaggaggaa
     6781 ttcaaaggtt tctcctactt tggtgaagac ctgatgccct gacacccaac ttttctatac
     6841 aaagtggttg atatccagca cagtggcggc cgctcgagtc tagagggccc gcggttcgaa
     6901 ggtaagccta tccctaaccc tctcctcggt ctcgattcta cgcgtaccgg ttagtaatga
     6961 gatccctccc ccccccctaa cgttactggc cgaagccgct tggaataagg ccggtgtgcg
     7021 tttgtctata tgttattttc caccatattg ccgtcttttg gcaatgtgag ggcccggaaa
     7081 cctggccctg tcttcttgac gagcattcct aggggtcttt cccctctcgc caaaggaatg
     7141 caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg aagacaaaca
     7201 acgtctgtag cgaccctttg caggcagcgg aaccccccac ctggcgacag gtgcctctgc
     7261 ggccaaaagc cacgtgtata agatacacct gcaaaggcgg cacaacccca gtgccacgtt
     7321 gtgagttgga tagttgtgga aagagtcaaa tggctctcct caagcgtatt caacaagggg
     7381 ctgaaggatg cccagaaggt accccattgt atgggatctg atctggggcc tcggtgcaca
     7441 tgctttacat gtgtttagtc gaggttaaaa aaacgtctag gccccccgaa ccacggggac
     7501 gtggttttcc tttgaaaaac acgatgataa tatggccaca catatgaccg agtacaagcc
     7561 cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc
     7621 gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt
     7681 caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt
     7741 cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc
     7801 ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca
     7861 gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc
     7921 caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc
     7981 cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg
     8041 caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga
     8101 aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc taaatcgata gatcctaatc
     8161 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
     8221 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
     8281 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
     8341 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
     8401 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg
     8461 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
     8521 gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct
     8581 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
     8641 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc
     8701 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctgag atcctttaag
     8761 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact
     8821 ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta ctgggtctct
     8881 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     8941 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     9001 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag
     9061 ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga
     9121 gaggcccggg ttaattaagg aaagggctag atcattcttg aagacgaaag ggcctcgtga
     9181 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca
     9241 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     9301 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     9361 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     9421 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     9481 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     9541 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     9601 cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     9661 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     9721 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     9781 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     9841 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     9901 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     9961 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
    10021 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
    10081 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
    10141 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
    10201 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
    10261 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
    10321 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
    10381 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
    10441 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
    10501 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
    10561 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
    10621 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
    10681 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
    10741 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
    10801 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
    10861 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
    10921 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
    10981 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
    11041 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
    11101 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
    11161 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagca
    11221 agctcatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
    11281 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc
    11341 cgtggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
    11401 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
    11461 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgacatg attacgaatt
    11521 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
    11581 tatcttatca tgtctggatc aactggataa ctcaagctaa ccaaaatcat cccaaacttc
    11641 ccaccccata ccctattacc actgccaatt acctgtggtt tcatttactc taaacctgtg
    11701 attcctctga attattttca ttttaaagaa attgtatttg ttaaatatgt actacaaact
    11761 tagtagt
//
