LOCUS       sequence-241117-        6098 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Targeting expression to neuronal and glial cell types in mice, 
            non-human primates, and human retina.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6098)
  AUTHORS   Juttner J, Szabo A, Gross-Scherf B, Morikawa RK, Rompani SB, Hantz 
            P, Szikra T, Esposti F, Cowan CS, Bharioke A, Patino-Alvarez CP, 
            Keles O, Kusnyerik A, Azoulay T, Hartl D, Krebs AR, Schubeler D, 
            Hajdu RI, Lukats A, Nemeth J, Nagy ZZ, Wu KC, Wu RH, Xiang L, Fang 
            XL, Jin ZB, Goldblum D, Hasler PW, Scholl HPN, Krol J, Roska B
  TITLE     Targeting neuronal and glial cell types with synthetic promoter AAVs
            in mice, non-human primates and humans.
  JOURNAL   Nat Neurosci. 2019 Jul 8. pii: 10.1038/s41593-019-0431-2. doi: 
            10.1038/s41593-019-0431-2.
  PUBMED    31285614
REFERENCE   2  (bases 1 to 6098)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2019 Jul 8. pii: 10.1038/s41593-019-0431-2. doi: 
            10.1038/s41593-019-0431-2."
COMMENT     Sequence Label: 161_pAAV-ProD5-CatCh-GFP-WPRE
FEATURES             Location/Qualifiers
     source          1..6098
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     promoter        457..488
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     regulatory      542..551
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             548..1474
                     /codon_start=1
                     /product="N-terminal fragment of channelrhodopsin-2 from 
                     the alga Chlamydomonas reinhardtii (Nagel et al., 2003)"
                     /label=ChR2
                     /note="blue light-activated cation channel"
                     /translation="MDYGGALSAVGRELLFVTNPVVVNGSVLVPEDQCYCAGWIESRGT
                     NGAQTASNVLQWLAAGFSILLLMFYAYQTWKSTCGWEEIYVCAIEMVKVILEFFFEFKN
                     PSMLYLATGHRVQWLRYAEWLLTCPVICIHLSNLTGLSNDYSRRTMGLLVSDIGTIVWG
                     ATSAMATGYVKVIFFCLGLCYGANTFFHAAKAYIEGYHTVPKGRCRQVVTGMAWLFFVS
                     WGMFPILFILGPEGFGVLSVYGSTVGHTIIDLMSKNCWGLLGHYLRVLIHEHILIHGDI
                     RKTTKLNIGGTEIEVETLVEDEAEAGAV"
     CDS             join(1478..1480,1481..1483,1484..2197)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1478..1480,1481..1483,1484..2194)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1523..1544)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1784..1803)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     misc_feature    2225..2813
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2278..2298)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2696..2707)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    2845..3321
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(3044..3063)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   3361..3501
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   3361..3490
                     /label=AAV2 ITR
     rep_origin      3576..4031
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3663..3682)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3873..3894
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4048..4067)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4167..4189
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4227..4245)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4313..4417
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4418..4486,4487..5278)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4636..4655)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5449..6037
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5938..5957
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtatcctg gggtttaaac aggactaggt ccactgggag
      181 aagaggaagt caaacaggac tagtcatcga taggctagcc agcttaatta atcagatttc
      241 caggaagagg caccagagcc ctctccctat tgctcaaagc gcaggtggaa taatcttggc
      301 cgtgtgaccg gaattcaaag cagttttggg aactgagccc caacctgtaa gccctgatgt
      361 tatttcaaga aagatggtgt cagaattaaa ttggattaga gaggaggata tggattagag
      421 cagagcagca tcctcacatg gtcctgctgg agttagtaga gggtatataa tggaagctcg
      481 acttccagct atcacatcca ctgtgttgtt gtgaactgga atccactata ggccaggatc
      541 cgccaccatg gattatggag gcgccctgag tgccgttggg cgcgagctgc tatttgtaac
      601 gaacccagta gtcgtcaatg gctctgtact tgtgcctgag gaccagtgtt actgcgcggg
      661 ctggattgag tcgcgtggca caaacggtgc ccaaacggcg tcgaacgtgc tgcaatggct
      721 tgctgctggc ttctccatcc tactgcttat gttttacgcc taccaaacat ggaagtcaac
      781 ctgcggctgg gaggagatct atgtgtgcgc tatcgagatg gtcaaggtga ttctcgagtt
      841 cttcttcgag tttaagaacc cgtccatgct gtatctagcc acaggccacc gcgtccagtg
      901 gttgcgttac gccgagtggc ttctcacctg cccggtcatt tgcattcacc tgtcaaacct
      961 gacgggcttg tccaacgact acagcaggcg caccatgggt ctgcttgtgt ctgatattgg
     1021 cacaattgtg tggggcgcca cttccgccat ggccaccgga tacgtcaagg tcatcttctt
     1081 ctgcctgggt ctgtgttatg gtgctaacac gttctttcac gctgccaagg cctacatcga
     1141 gggttaccac accgtgccga agggccggtg tcgccaggtg gtgactggca tggcttggct
     1201 cttcttcgta tcatggggta tgttccccat cctgttcatc ctcggccccg agggcttcgg
     1261 cgtcctgagc gtgtacggct ccaccgtcgg ccacaccatc attgacctga tgtcgaagaa
     1321 ctgctggggt ctgctcggcc actacctgcg cgtgctgatc cacgagcata tcctcatcca
     1381 cggcgacatt cgcaagacca ccaaattgaa cattggtggc actgagattg aggtcgagac
     1441 gctggtggag gacgaggccg aggctggcgc ggtacccatg gtgagcaagg gcgaggagct
     1501 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
     1561 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
     1621 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg
     1681 cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc
     1741 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
     1801 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
     1861 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag
     1921 ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat
     1981 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
     2041 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct
     2101 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
     2161 cgggatcact ctcggcatgg acgagctgta caagtgataa gaattcgata tcaagcttat
     2221 cgataatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
     2281 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
     2341 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga
     2401 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc
     2461 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct
     2521 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
     2581 gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct
     2641 gctcgcctat gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc
     2701 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg
     2761 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcatcgata
     2821 ccgagcgctg ctcgagagat ctacgggtgg catccctgtg acccctcccc agtgcctctc
     2881 ctggccctgg aagttgccac tccagtgccc accagccttg tcctaataaa attaagttgc
     2941 atcattttgt ctgactaggt gtccttctat aatattatgg ggtggagggg ggtggtatgg
     3001 agcaaggggc aagttgggaa gacaacctgt agggcctgcg gggtctattg ggaaccaagc
     3061 tggagtgcag tggcacaatc ttggctcact gcaatctccg cctcctgggt tcaagcgatt
     3121 ctcctgcctc agcctcccga gttgttggga ttccaggcat gcatgaccag gctcagctaa
     3181 tttttgtttt tttggtagag acggggtttc accatattgg ccaggctggt ctccaactcc
     3241 taatctcagg tgatctaccc accttggcct cccaaattgc tgggattaca ggcgtgaacc
     3301 actgctccct tccctgtcct tctgattttg taggtaacca cgtgcggacc gagcggccgc
     3361 aggaacccct agtgatggag ttggccactc cctctctgcg cgctcgctcg ctcactgagg
     3421 ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc
     3481 gagcgcgcag ctgcctgcag gggcgcctga tgcggtattt tctccttacg catctgtgcg
     3541 gtatttcaca ccgcatacgt caaagcaacc atagtacgcg ccctgtagcg gcgcattaag
     3601 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     3661 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     3721 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     3781 aaaacttgat ttgggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     3841 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     3901 actcaaccct atctcgggct attcttttga tttataaggg attttgccga tttcggccta
     3961 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac
     4021 gtttacaatt ttatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
     4081 gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc
     4141 cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc
     4201 atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt
     4261 catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac
     4321 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     4381 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     4441 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     4501 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     4561 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     4621 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
     4681 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     4741 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     4801 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     4861 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     4921 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     4981 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     5041 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     5101 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     5161 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     5221 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     5281 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     5341 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     5401 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     5461 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     5521 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     5581 gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     5641 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     5701 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     5761 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     5821 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     5881 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     5941 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     6001 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     6061 acggttcctg gccttttgct ggccttttgc tcacatgt
//
