LOCUS       sequence-241123-        6631 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Targeting expression to neuronal and glial cell types in mice, 
            non-human primates, and human retina.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6631)
  AUTHORS   Juttner J, Szabo A, Gross-Scherf B, Morikawa RK, Rompani SB, Hantz 
            P, Szikra T, Esposti F, Cowan CS, Bharioke A, Patino-Alvarez CP, 
            Keles O, Kusnyerik A, Azoulay T, Hartl D, Krebs AR, Schubeler D, 
            Hajdu RI, Lukats A, Nemeth J, Nagy ZZ, Wu KC, Wu RH, Xiang L, Fang 
            XL, Jin ZB, Goldblum D, Hasler PW, Scholl HPN, Krol J, Roska B
  TITLE     Targeting neuronal and glial cell types with synthetic promoter AAVs
            in mice, non-human primates and humans.
  JOURNAL   Nat Neurosci. 2019 Jul 8. pii: 10.1038/s41593-019-0431-2. doi: 
            10.1038/s41593-019-0431-2.
  PUBMED    31285614
REFERENCE   2  (bases 1 to 6631)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2019 Jul 8. pii: 10.1038/s41593-019-0431-2. doi: 
            10.1038/s41593-019-0431-2."
COMMENT     Sequence Label: 167_pAAV-ProC34-CatCh-GFP-WPRE
FEATURES             Location/Qualifiers
     source          1..6631
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     promoter        872..1068
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      919..1054
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     regulatory      1075..1084
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1081..2007
                     /codon_start=1
                     /product="N-terminal fragment of channelrhodopsin-2 from 
                     the alga Chlamydomonas reinhardtii (Nagel et al., 2003)"
                     /label=ChR2
                     /note="blue light-activated cation channel"
                     /translation="MDYGGALSAVGRELLFVTNPVVVNGSVLVPEDQCYCAGWIESRGT
                     NGAQTASNVLQWLAAGFSILLLMFYAYQTWKSTCGWEEIYVCAIEMVKVILEFFFEFKN
                     PSMLYLATGHRVQWLRYAEWLLTCPVICIHLSNLTGLSNDYSRRTMGLLVSDIGTIVWG
                     ATSAMATGYVKVIFFCLGLCYGANTFFHAAKAYIEGYHTVPKGRCRQVVTGMAWLFFVS
                     WGMFPILFILGPEGFGVLSVYGSTVGHTIIDLMSKNCWGLLGHYLRVLIHEHILIHGDI
                     RKTTKLNIGGTEIEVETLVEDEAEAGAV"
     CDS             join(2011..2013,2014..2016,2017..2730)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2011..2013,2014..2016,2017..2727)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2056..2077)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2317..2336)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2664..2685
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    2758..3346
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2811..2831)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3229..3240)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3378..3854
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(3577..3596)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   3894..4034
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   3894..4023
                     /label=AAV2 ITR
     rep_origin      4109..4564
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4196..4215)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4406..4427
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4581..4600)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4700..4722
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4760..4778)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4846..4950
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4951..5019,5020..5811)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5169..5188)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5982..6570
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6471..6490
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgttatcat gccatggttt gatttgcata aatactagcc
      181 gactccaaaa tcaattaatt tgtacctcaa tacagcatgc ataaattcat tggttccgat
      241 tccagaatgt acatgtaaaa ttcgatagaa ttgtgacagg tgtgaaggtt cagtagctcc
      301 atgatttgca tacccatcag ttcccgacaa aatcaattaa tttatggtca gtacttacat
      361 gcataaattc atacaacaag cacaaagaat gtacatgtaa aattgatctt ttatcagtta
      421 ggtgtgaaaa cacgtccata ccgtgatttg catacacggc tcgtcctacc aaaatcaatt
      481 aatttagcat aaaatagaac atgcataaat tcatttgtac tacacacaaa atgtacatgt
      541 aaaattacct tgaccctaaa taggtgtgaa gtgagagtga tcttttgatt tgcatatagt
      601 cccgtcgtac gcaaaatcaa ttaattttgt cgagttgtat atatgcataa attcattacg
      661 gagacgaatc caatgtacat gtaaaattat aaaagatatc tcaaggtgtg aatgggctgc
      721 actccaatga tttgcatatg gtagcgtgct taacaaaatc aattaatttt tcagactgat
      781 tttgatgcat aaattcatga acgcccgttc acgaatgtac atgtaaaatt actttattca
      841 cgtcgaggtg tgaagctcga gatctgcgat ctgcatctca attagtcagc aaccatagtc
      901 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
      961 catcgctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     1021 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaagg atccgccacc
     1081 atggattatg gaggcgccct gagtgccgtt gggcgcgagc tgctatttgt aacgaaccca
     1141 gtagtcgtca atggctctgt acttgtgcct gaggaccagt gttactgcgc gggctggatt
     1201 gagtcgcgtg gcacaaacgg tgcccaaacg gcgtcgaacg tgctgcaatg gcttgctgct
     1261 ggcttctcca tcctactgct tatgttttac gcctaccaaa catggaagtc aacctgcggc
     1321 tgggaggaga tctatgtgtg cgctatcgag atggtcaagg tgattctcga gttcttcttc
     1381 gagtttaaga acccgtccat gctgtatcta gccacaggcc accgcgtcca gtggttgcgt
     1441 tacgccgagt ggcttctcac ctgcccggtc atttgcattc acctgtcaaa cctgacgggc
     1501 ttgtccaacg actacagcag gcgcaccatg ggtctgcttg tgtctgatat tggcacaatt
     1561 gtgtggggcg ccacttccgc catggccacc ggatacgtca aggtcatctt cttctgcctg
     1621 ggtctgtgtt atggtgctaa cacgttcttt cacgctgcca aggcctacat cgagggttac
     1681 cacaccgtgc cgaagggccg gtgtcgccag gtggtgactg gcatggcttg gctcttcttc
     1741 gtatcatggg gtatgttccc catcctgttc atcctcggcc ccgagggctt cggcgtcctg
     1801 agcgtgtacg gctccaccgt cggccacacc atcattgacc tgatgtcgaa gaactgctgg
     1861 ggtctgctcg gccactacct gcgcgtgctg atccacgagc atatcctcat ccacggcgac
     1921 attcgcaaga ccaccaaatt gaacattggt ggcactgaga ttgaggtcga gacgctggtg
     1981 gaggacgagg ccgaggctgg cgcggtaccc atggtgagca agggcgagga gctgttcacc
     2041 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg
     2101 tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc
     2161 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag
     2221 tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc
     2281 gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc
     2341 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac
     2401 ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac
     2461 gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac
     2521 aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc
     2581 gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa
     2641 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc
     2701 actctcggca tggacgagct gtacaagtga taagaattcg atatcaagct tatcgataat
     2761 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     2821 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     2881 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     2941 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     3001 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     3061 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     3121 ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc
     3181 tatgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     3241 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
     3301 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcatcg ataccgagcg
     3361 ctgctcgaga gatctacggg tggcatccct gtgacccctc cccagtgcct ctcctggccc
     3421 tggaagttgc cactccagtg cccaccagcc ttgtcctaat aaaattaagt tgcatcattt
     3481 tgtctgacta ggtgtccttc tataatatta tggggtggag gggggtggta tggagcaagg
     3541 ggcaagttgg gaagacaacc tgtagggcct gcggggtcta ttgggaacca agctggagtg
     3601 cagtggcaca atcttggctc actgcaatct ccgcctcctg ggttcaagcg attctcctgc
     3661 ctcagcctcc cgagttgttg ggattccagg catgcatgac caggctcagc taatttttgt
     3721 ttttttggta gagacggggt ttcaccatat tggccaggct ggtctccaac tcctaatctc
     3781 aggtgatcta cccaccttgg cctcccaaat tgctgggatt acaggcgtga accactgctc
     3841 ccttccctgt ccttctgatt ttgtaggtaa ccacgtgcgg accgagcggc cgcaggaacc
     3901 cctagtgatg gagttggcca ctccctctct gcgcgctcgc tcgctcactg aggccgggcg
     3961 accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg
     4021 cagctgcctg caggggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc
     4081 acaccgcata cgtcaaagca accatagtac gcgccctgta gcggcgcatt aagcgcggcg
     4141 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     4201 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     4261 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     4321 gatttgggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     4381 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     4441 cctatctcgg gctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     4501 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgtttaca
     4561 attttatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga
     4621 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
     4681 agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg
     4741 aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
     4801 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     4861 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     4921 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     4981 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     5041 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     5101 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     5161 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     5221 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     5281 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     5341 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     5401 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     5461 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     5521 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     5581 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     5641 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     5701 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     5761 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     5821 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     5881 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     5941 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     6001 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     6061 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     6121 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     6181 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     6241 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     6301 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     6361 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     6421 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     6481 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     6541 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     6601 ctggcctttt gctggccttt tgctcacatg t
//
