LOCUS       sequence-241137-        6408 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Targeting expression to neuronal and glial cell types in mice, 
            non-human primates, and human retina.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6408)
  AUTHORS   Juttner J, Szabo A, Gross-Scherf B, Morikawa RK, Rompani SB, Hantz 
            P, Szikra T, Esposti F, Cowan CS, Bharioke A, Patino-Alvarez CP, 
            Keles O, Kusnyerik A, Azoulay T, Hartl D, Krebs AR, Schubeler D, 
            Hajdu RI, Lukats A, Nemeth J, Nagy ZZ, Wu KC, Wu RH, Xiang L, Fang 
            XL, Jin ZB, Goldblum D, Hasler PW, Scholl HPN, Krol J, Roska B
  TITLE     Targeting neuronal and glial cell types with synthetic promoter AAVs
            in mice, non-human primates and humans.
  JOURNAL   Nat Neurosci. 2019 Jul 8. pii: 10.1038/s41593-019-0431-2. doi: 
            10.1038/s41593-019-0431-2.
  PUBMED    31285614
REFERENCE   2  (bases 1 to 6408)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2019 Jul 8. pii: 10.1038/s41593-019-0431-2. doi: 
            10.1038/s41593-019-0431-2."
COMMENT     Sequence Label: 180_pAAV-ProD11-CatCh-GFP-WPRE
FEATURES             Location/Qualifiers
     source          1..6408
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     promoter        767..798
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     regulatory      852..861
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             858..1784
                     /codon_start=1
                     /product="N-terminal fragment of channelrhodopsin-2 from 
                     the alga Chlamydomonas reinhardtii (Nagel et al., 2003)"
                     /label=ChR2
                     /note="blue light-activated cation channel"
                     /translation="MDYGGALSAVGRELLFVTNPVVVNGSVLVPEDQCYCAGWIESRGT
                     NGAQTASNVLQWLAAGFSILLLMFYAYQTWKSTCGWEEIYVCAIEMVKVILEFFFEFKN
                     PSMLYLATGHRVQWLRYAEWLLTCPVICIHLSNLTGLSNDYSRRTMGLLVSDIGTIVWG
                     ATSAMATGYVKVIFFCLGLCYGANTFFHAAKAYIEGYHTVPKGRCRQVVTGMAWLFFVS
                     WGMFPILFILGPEGFGVLSVYGSTVGHTIIDLMSKNCWGLLGHYLRVLIHEHILIHGDI
                     RKTTKLNIGGTEIEVETLVEDEAEAGAV"
     CDS             join(1788..1790,1791..1793,1794..2507)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1788..1790,1791..1793,1794..2504)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1833..1854)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2094..2113)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     misc_feature    2535..3123
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2588..2608)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3006..3017)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3155..3631
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(3354..3373)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   3671..3811
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   3671..3800
                     /label=AAV2 ITR
     rep_origin      3886..4341
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3973..3992)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4183..4204
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4358..4377)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4477..4499
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4537..4555)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4623..4727
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4728..4796,4797..5588)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4946..4965)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5759..6347
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6248..6267
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtatcctg gggtttaaac aggactaggt ccactgggag
      181 aagaggaagt caaacaggac tagtcatcga taggctagcc agcttaatta atcagatgca
      241 ggagtttgag ggatgcggat gaataggttg agggttgagg accactgcgc ctctgatggt
      301 tgtctagagg cagacaatac gcagtctgga gctggagcag gtgcgtgcag tgtgcattgg
      361 ggcttctgcc agtgatccaa gataggaaga catagtgagt gcttagaaga gcaccagcat
      421 gtgaagggct gagaagtggt cggcgaggaa aggccctcaa aggagcctca gagggagtgg
      481 acacagagag gcttaaagtt aagagcatcg agaagacagc tcttcgtgga aggggtgtgg
      541 cctccagtta ggttgttggg gaattgtaga gataaatgaa agtatccgtt agacttgcaa
      601 aggcgatgac aaaaataaac ctggttaatg ctgtcagtgg tttgaaagtg agcaagaaga
      661 ttgcaccaga ttaaagagca agtgagcagt ctgttaaatg tacatgtctg ttggtaggag
      721 catggccacc cttgaagcaa tcctcacatg gtcctgctgg agttagtaga gggtatataa
      781 tggaagctcg acttccagct atcacatcca ctgtgttgtt gtgaactgga atccactata
      841 ggccaggatc cgccaccatg gattatggag gcgccctgag tgccgttggg cgcgagctgc
      901 tatttgtaac gaacccagta gtcgtcaatg gctctgtact tgtgcctgag gaccagtgtt
      961 actgcgcggg ctggattgag tcgcgtggca caaacggtgc ccaaacggcg tcgaacgtgc
     1021 tgcaatggct tgctgctggc ttctccatcc tactgcttat gttttacgcc taccaaacat
     1081 ggaagtcaac ctgcggctgg gaggagatct atgtgtgcgc tatcgagatg gtcaaggtga
     1141 ttctcgagtt cttcttcgag tttaagaacc cgtccatgct gtatctagcc acaggccacc
     1201 gcgtccagtg gttgcgttac gccgagtggc ttctcacctg cccggtcatt tgcattcacc
     1261 tgtcaaacct gacgggcttg tccaacgact acagcaggcg caccatgggt ctgcttgtgt
     1321 ctgatattgg cacaattgtg tggggcgcca cttccgccat ggccaccgga tacgtcaagg
     1381 tcatcttctt ctgcctgggt ctgtgttatg gtgctaacac gttctttcac gctgccaagg
     1441 cctacatcga gggttaccac accgtgccga agggccggtg tcgccaggtg gtgactggca
     1501 tggcttggct cttcttcgta tcatggggta tgttccccat cctgttcatc ctcggccccg
     1561 agggcttcgg cgtcctgagc gtgtacggct ccaccgtcgg ccacaccatc attgacctga
     1621 tgtcgaagaa ctgctggggt ctgctcggcc actacctgcg cgtgctgatc cacgagcata
     1681 tcctcatcca cggcgacatt cgcaagacca ccaaattgaa cattggtggc actgagattg
     1741 aggtcgagac gctggtggag gacgaggccg aggctggcgc ggtacccatg gtgagcaagg
     1801 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg
     1861 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
     1921 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc
     1981 tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct
     2041 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg
     2101 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
     2161 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca
     2221 actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga
     2281 acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc
     2341 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc
     2401 agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg
     2461 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtgataa gaattcgata
     2521 tcaagcttat cgataatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     2581 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     2641 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
     2701 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
     2761 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
     2821 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
     2881 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct
     2941 ttccttggct gctcgcctat gttgccacct ggattctgcg cgggacgtcc ttctgctacg
     3001 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
     3061 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
     3121 cgcatcgata ccgagcgctg ctcgagagat ctacgggtgg catccctgtg acccctcccc
     3181 agtgcctctc ctggccctgg aagttgccac tccagtgccc accagccttg tcctaataaa
     3241 attaagttgc atcattttgt ctgactaggt gtccttctat aatattatgg ggtggagggg
     3301 ggtggtatgg agcaaggggc aagttgggaa gacaacctgt agggcctgcg gggtctattg
     3361 ggaaccaagc tggagtgcag tggcacaatc ttggctcact gcaatctccg cctcctgggt
     3421 tcaagcgatt ctcctgcctc agcctcccga gttgttggga ttccaggcat gcatgaccag
     3481 gctcagctaa tttttgtttt tttggtagag acggggtttc accatattgg ccaggctggt
     3541 ctccaactcc taatctcagg tgatctaccc accttggcct cccaaattgc tgggattaca
     3601 ggcgtgaacc actgctccct tccctgtcct tctgattttg taggtaacca cgtgcggacc
     3661 gagcggccgc aggaacccct agtgatggag ttggccactc cctctctgcg cgctcgctcg
     3721 ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca
     3781 gtgagcgagc gagcgcgcag ctgcctgcag gggcgcctga tgcggtattt tctccttacg
     3841 catctgtgcg gtatttcaca ccgcatacgt caaagcaacc atagtacgcg ccctgtagcg
     3901 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     3961 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     4021 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     4081 tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag tgggccatcg ccctgataga
     4141 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     4201 ctggaacaac actcaaccct atctcgggct attcttttga tttataaggg attttgccga
     4261 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
     4321 aaatattaac gtttacaatt ttatggtgca ctctcagtac aatctgctct gatgccgcat
     4381 agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
     4441 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
     4501 tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat
     4561 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
     4621 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     4681 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     4741 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     4801 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     4861 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     4921 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     4981 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     5041 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     5101 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     5161 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     5221 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     5281 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     5341 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     5401 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     5461 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     5521 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     5581 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     5641 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     5701 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     5761 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     5821 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     5881 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca
     5941 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     6001 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     6061 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     6121 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     6181 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     6241 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     6301 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     6361 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgt
//
